Restriction map for
pFN21AA0619
(Circular) (Six-base) MAPSORT of: pFN21AA0619.seq Check: 7261 from: 1 to: 8869
pFN21AA0619 8869 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6679 278
Size: 53 83 186 936 5143 2468
Fragments arranged by size:
5143 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2257 3353 6142 6212 1937
Size: 320 1096 2789 70 4594
Fragments arranged by size:
4594 2789 1096 320 70
Acc65I G'GTAC_C
Cuts at: 6190 6190
Size: 8869
AclI AA'CG_TT
Cuts at: 1563 6996 7369 1563
Size: 5433 373 3063
Fragments arranged by size:
5433 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 2973 3512 3857 4004 4454 4821 5313
Size: 981 360 539 345 147 450 367 492
Cuts at: 5313 6932 8316 8584 1632
Size: 1619 1384 268 1917
Fragments arranged by size:
1917 1619 1384 981 539 492 450 367
360 345 268 147
AfeI AGC'GCT
Cuts at: 4412 4412
Size: 8869
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8672
AflIII A'CryG_T
Cuts at: 1203 2708 5853 7768 8745 1203
Size: 1505 3145 1915 977 1327
Fragments arranged by size:
3145 1915 1505 1327 977
AgeI A'CCGG_T
Cuts at: 7700 7700
Size: 8869
AhdI GACnn_n'nnGTC
Cuts at: 7598 7598
Size: 8869
AleI CACnn'nnGTG
Cuts at: 1166 5168 1166
Size: 4002 4867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3398 3555 1184
Size: 2214 157 6498
Fragments arranged by size:
6498 2214 157
AlwNI CAG_nnn'CTG
Cuts at: 2405 3113 3728 3787 4775 5695 6612 8184 2405
Size: 708 615 59 988 920 917 1572 3090
Fragments arranged by size:
3090 1572 988 920 917 708 615 59
ApaLI G'TGCA_C
Cuts at: 6926 8082 6926
Size: 1156 7713
ApoI r'AATT_y
Cuts at: 1493 2155 2579 2935 2964 3259 3586 3860 5504
Size: 662 424 356 29 295 327 274 1644
Cuts at: 5504 5673 5732 5998 6178 6404 6556 8665 1493
Size: 169 59 266 180 226 152 2109 1697
Fragments arranged by size:
2109 1697 1644 662 424 356 327 295
274 266 226 180 169 152 59 29
AseI AT'TA_AT
Cuts at: 160 1032 3927 7423 160
Size: 872 2895 3496 1606
Fragments arranged by size:
3496 2895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8869
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 6194 1870
Size: 73 102 4149 4545
Fragments arranged by size:
4545 4149 102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2739 2772 2739
Size: 33 8836
BamHI G'GATC_C
Cuts at: 6199 6566 6199
Size: 367 8502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 6190 7645 618
Size: 325 217 627 252 4151 1455 1842
Fragments arranged by size:
4151 1842 1455 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 4338 4590 6188 1391
Size: 2947 252 1598 4072
Fragments arranged by size:
4072 2947 1598 252
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 8869
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 928
Size: 971 381 7517
Fragments arranged by size:
7517 971 381
BbvCI CC'TCA_GC
Cuts at: 5430 5430
Size: 8869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 3136 3170 4769 4803 7060
Size: 34 375 34 790 34 1599 34 2257
Cuts at: 7060 7094 1903
Size: 34 3678
Fragments arranged by size:
3678 2257 1599 790 375 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2869 6761 7971 2869
Size: 3892 1210 3767
Fragments arranged by size:
3892 3767 1210
BclI T'GATC_A
Cuts at: 1548 3073 3562 5247 1548
Size: 1525 489 1685 5170
Fragments arranged by size:
5170 1685 1525 489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4139 5848 7480
Size: 107 122 71 631 605 2467 1709 1632
Cuts at: 7480 136
Size: 1525
Fragments arranged by size:
2467 1709 1632 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3805 4164 8864 3805
Size: 359 4700 3810
Fragments arranged by size:
4700 3810 359
BlpI GC'TnA_GC
Cuts at: 6286 6286
Size: 8869
Bme1580I G_kGCm'C
Cuts at: 1790 6930 8086 1790
Size: 5140 1156 2573
Fragments arranged by size:
5140 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6600 7558 442
Size: 416 53 83 399 24 5183 958 1753
Fragments arranged by size:
5183 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3668 3851 4025 4574 4769 4868 6051
Size: 875 1554 183 174 549 195 99 1183
Cuts at: 6051 7529 1239
Size: 1478 2579
Fragments arranged by size:
2579 1554 1478 1183 875 549 195 183
174 99
Bpu10I CC'TnA_GC
Cuts at: 5430 5430
Size: 8869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4937 5085 6994 7859 8157 4937
Size: 148 1909 865 298 5649
Fragments arranged by size:
5649 1909 865 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2103 2523 7532 882
Size: 617 543 61 420 5009 2219
Fragments arranged by size:
5009 2219 617 543 420 61
BsaAI yAC'GTr
Cuts at: 493 1204 2709 6660 493
Size: 711 1505 3951 2702
Fragments arranged by size:
3951 2702 1505 711
BsaBI GATnn'nnATC
Cuts at: 2493 8863 2493
Size: 6370 2499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 6676 7058 7693
Size: 53 83 186 936 507 4636 382 635
Cuts at: 7693 275
Size: 1451
Fragments arranged by size:
4636 1451 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2764 7302 7700 7974 8121 8532 2764
Size: 4538 398 274 147 411 3101
Fragments arranged by size:
4538 3101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 2520 2550 3094
Size: 30 165 30 699 30 388 30 544
Cuts at: 3094 3124 1178
Size: 30 6923
Fragments arranged by size:
6923 699 544 388 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3476 5856 5945 1186
Size: 2290 2380 89 4110
Fragments arranged by size:
4110 2380 2290 89
BseYI C'CCAG_C
Cuts at: 5238 6061 8072 8568 5238
Size: 823 2011 496 5539
Fragments arranged by size:
5539 2011 823 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 7690
Fragments arranged by size:
7690 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6319 7080 8108 1540
Size: 275 186 4318 761 1028 2301
Fragments arranged by size:
4318 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4338 4590 6930 7015 8086 4338
Size: 252 2340 85 1071 5121
Fragments arranged by size:
5121 2340 1071 252 85
BsmI GAATG_Cn'
Cuts at: 6380 6473 6380
Size: 93 8776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4338 4590 6188 6930 7015 8086 1391
Size: 399 2548 252 1598 742 85 1071 2174
Fragments arranged by size:
2548 2174 1598 1071 742 399 252 85
BspEI T'CCGG_A
Cuts at: 8532 8532
Size: 8869
BspHI T'CATG_A
Cuts at: 3565 4836 5127 6758 8488 3565
Size: 1271 291 1631 1730 3946
Fragments arranged by size:
3946 1730 1631 1271 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6224 844
Size: 5380 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6756 1394
Size: 429 4933 3507
Fragments arranged by size:
4933 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3382 5477 7364 7538 65
Size: 1183 2134 2095 1887 174 1396
Fragments arranged by size:
2134 2095 1887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 7513 7700 1700
Size: 110 200 5503 187 2869
Fragments arranged by size:
5503 2869 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 2499 96
Size: 2403 6466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8869
BssSI C'ACGA_G
Cuts at: 6929 7941 6929
Size: 1012 7857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8869
BstBI TT'CG_AA
Cuts at: 4549 5308 7673 4549
Size: 759 2365 5745
Fragments arranged by size:
5745 2365 759
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8869
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2131
Size: 8869
BstYI r'GATC_y
Cuts at: 1972 3805 4164 6199 6566 6951 6968 8420 8506
Size: 1833 359 2035 367 385 17 1452 86
Cuts at: 8506 8518 8864 1972
Size: 12 346 1977
Fragments arranged by size:
2035 1977 1833 1452 385 367 359 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3354 3354
Size: 8869
Bsu36I CC'TnA_GG
Cuts at: 2781 2781
Size: 8869
BtgI C'CryG_G
Cuts at: 513 1065 2625 3910 513
Size: 552 1560 1285 5472
Fragments arranged by size:
5472 1560 1285 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2328 487
Size: 683 56 1102 7028
Fragments arranged by size:
7028 1102 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 3139 5419 6385 7179 7199 2586
Size: 553 2280 966 794 20 4256
Fragments arranged by size:
4256 2280 966 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8834
DraI TTT'AAA
Cuts at: 3969 4242 6173 3969
Size: 273 1931 6665
Fragments arranged by size:
6665 1931 273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6138 7876 809
Size: 507 4822 1738 1802
Fragments arranged by size:
4822 1802 1738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6134 6316 7205
Size: 54 1425 50 275 18 4304 182 889
Cuts at: 7205 8834 8
Size: 1629 43
Fragments arranged by size:
4304 1629 1425 889 275 182 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6316 1537
Size: 275 4504 4090
Fragments arranged by size:
4504 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2296 3453 4004 5289 6003 6799 1353
Size: 943 1157 551 1285 714 796 3423
Fragments arranged by size:
3423 1285 1157 943 796 714 551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7456 7830 7976 387
Size: 1064 138 5867 374 146 1280
Fragments arranged by size:
5867 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4336 4588 4336
Size: 252 8617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2613 2973 3512 3668 3851 3857
Size: 393 482 499 360 539 156 183 6
Cuts at: 3857 4004 4025 4454 4574 4769 4821 4868 5313
Size: 147 21 429 120 195 52 47 445
Cuts at: 5313 6051 6932 7529 8316 8584 1239
Size: 738 881 597 787 268 1524
Fragments arranged by size:
1524 881 787 738 597 539 499 482
445 429 393 360 268 195 183 156
147 120 52 47 21 6
EcoRI G'AATT_C
Cuts at: 2935 6178 6556 2935
Size: 3243 378 5248
Fragments arranged by size:
5248 3243 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2325 2618 3386 3638 4240 5489 2325
Size: 293 768 252 602 1249 5705
Fragments arranged by size:
5705 1249 768 602 293 252
FspI TGC'GCA
Cuts at: 7375 7375
Size: 8869
HaeII r_GCGC'y
Cuts at: 1678 2043 2102 3524 4414 8016 1678
Size: 365 59 1422 890 3602 2531
Fragments arranged by size:
3602 2531 1422 890 365 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2759 2791 2822
Size: 32 135 32 530 32 625 32 31
Cuts at: 2822 2854 3266 3298 4997 5029 5116 5148 5201
Size: 32 412 32 1699 32 87 32 53
Cuts at: 5201 5233 5239 5271 7510 7542 7584 7616 1373
Size: 32 6 32 2239 32 42 32 2626
Fragments arranged by size:
2626 2239 1699 625 530 412 135 87
53 42 32 32 32 32 32 32
32 32 32 32 32 32 31 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3604 4456 6143 6213 6459 669
Size: 988 281 1666 852 1687 70 246 3079
Fragments arranged by size:
3079 1687 1666 988 852 281 246 70
HindIII A'AGCT_T
Cuts at: 748 2366 3207 748
Size: 1618 841 6410
Fragments arranged by size:
6410 1618 841
HpaI GTT'AAC
Cuts at: 3604 4456 6459 3604
Size: 852 2003 6014
Fragments arranged by size:
6014 2003 852
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 8869
KpnI G_GTAC'C
Cuts at: 6194 6194
Size: 8869
MfeI C'AATT_G
Cuts at: 4794 4905 6468 4794
Size: 111 1563 7195
Fragments arranged by size:
7195 1563 111
MluI A'CGCG_T
Cuts at: 8745 8745
Size: 8869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 3196 5653 5731 7983 8167 1305
Size: 1432 459 2457 78 2252 184 2007
Fragments arranged by size:
2457 2252 2007 1432 459 184 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7047
Fragments arranged by size:
7047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2507 2643 2711 3537 4511 5168
Size: 648 963 378 136 68 826 974 657
Cuts at: 5168 6827 7186 7345 518
Size: 1659 359 159 2042
Fragments arranged by size:
2042 1659 974 963 826 657 648 378
359 159 136 68
MspA1I CmG'CkG
Cuts at: 1606 3226 3787 5359 6285 6962 8110 8355 1606
Size: 1620 561 1572 926 677 1148 245 2120
Fragments arranged by size:
2120 1620 1572 1148 926 677 561 245
NaeI GCC'GGC
Cuts at: 2012 2012
Size: 8869
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 8869
NcoI C'CATG_G
Cuts at: 513 1065 2625 3910 513
Size: 552 1560 1285 5472
Fragments arranged by size:
5472 1560 1285 552
NdeI CA'TA_TG
Cuts at: 387 5205 5916 387
Size: 4818 711 3340
Fragments arranged by size:
4818 3340 711
NgoMIV G'CCGG_C
Cuts at: 2010 2010
Size: 8869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8869
NotI GC'GGCC_GC
Cuts at: 6316 6316
Size: 8869
NspI r_CATG'y
Cuts at: 1132 1229 2521 2665 2978 5857 6227 7772 1132
Size: 97 1292 144 313 2879 370 1545 2229
Fragments arranged by size:
2879 2229 1545 1292 370 313 144 97
PasI CC'CwG_GG
Cuts at: 1348 2454 1348
Size: 1106 7763
PciI A'CATG_T
Cuts at: 5853 7768 5853
Size: 1915 6954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4507 1108
Size: 3399 5470
PfoI T'CCnGG_A
Cuts at: 4590 4590
Size: 8869
PmeI GTTT'AAAC
Cuts at: 6173 6173
Size: 8869
PmlI CAC'GTG
Cuts at: 2709 2709
Size: 8869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6938 6970 8451 8483 6938
Size: 32 1481 32 7324
Fragments arranged by size:
7324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8869
PsiI TTA'TAA
Cuts at: 4632 6439 4632
Size: 1807 7062
PstI C_TGCA'G
Cuts at: 830 3739 5322 6221 830
Size: 2909 1583 899 3478
Fragments arranged by size:
3478 2909 1583 899
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8869
PvuII CAG'CTG
Cuts at: 3226 3787 5359 3226
Size: 561 1572 6736
Fragments arranged by size:
6736 1572 561
SacI G_AGCT'C
Cuts at: 4338 4590 4338
Size: 252 8617
SalI G'TCGA_C
Cuts at: 1936 6141 6211 1936
Size: 4205 70 4594
Fragments arranged by size:
4594 4205 70
SapI GCTCTTCn'nnn_
Cuts at: 6003 6003
Size: 8869
SbfI CC_TGCA'GG
Cuts at: 6221 6221
Size: 8869
ScaI AGT'ACT
Cuts at: 7117 8829 7117
Size: 1712 7157
SfcI C'TryA_G
Cuts at: 826 1045 2258 2841 3114 3250 3735 5318 6003
Size: 219 1213 583 273 136 485 1583 685
Cuts at: 6003 6217 7352 8033 8224 826
Size: 214 1135 681 191 1471
Fragments arranged by size:
1583 1471 1213 1135 685 681 583 485
273 219 214 191 136
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 8869
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8869
SmaI CCC'GGG
Cuts at: 1872 6196 1872
Size: 4324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4916 5100 6973 7874 8136 820
Size: 197 926 2973 184 1873 901 262 1553
Fragments arranged by size:
2973 1873 1553 926 901 262 197 184
SnaBI TAC'GTA
Cuts at: 493 6660 493
Size: 6167 2702
SpeI A'CTAG_T
Cuts at: 152 4940 152
Size: 4788 4081
SphI G_CATG'C
Cuts at: 1132 6227 1132
Size: 5095 3774
SspI AAT'ATT
Cuts at: 5 52 4811 6793 5
Size: 47 4759 1982 2081
Fragments arranged by size:
4759 2081 1982 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8869
StyI C'CwwG_G
Cuts at: 513 1065 2160 2625 3910 5678 6308 513
Size: 552 1095 465 1285 1768 630 3074
Fragments arranged by size:
3074 1768 1285 1095 630 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2446 6896 6913 7066 7251 7696 325
Size: 1255 866 4450 17 153 185 445 1498
Fragments arranged by size:
4450 1498 1255 866 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 2514 7115
Size: 274 80 33 51 1443 522 15 4601
Cuts at: 7115 8827 96
Size: 1712 138
Fragments arranged by size:
4601 1712 1443 522 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 3395 3427 8829 8861 2931
Size: 32 432 32 5402 32 2939
Fragments arranged by size:
5402 2939 432 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8869
XbaI T'CTAG_A
Cuts at: 3883 6205 3883
Size: 2322 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3397 4207 5593 3397
Size: 810 1386 6673
Fragments arranged by size:
6673 1386 810
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8869
XmaI C'CCGG_G
Cuts at: 1870 6194 1870
Size: 4324 4545
XmnI GAAnn'nnTTC
Cuts at: 2598 6998 2598
Size: 4400 4469
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6677 276
Size: 53 83 186 936 5143 2468
Fragments arranged by size:
5143 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BfrBI BplI BsiWI
BsmBI DraIII EcoNI EcoO109I EcoRV FseI FspAI NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI