Restriction map for
pFN21AA0603
(Circular) (Six-base) MAPSORT of: pFN21AA0603.seq Check: 9660 from: 1 to: 8602
pFN21AA0603 8602 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6412 278
Size: 53 83 186 936 4876 2468
Fragments arranged by size:
4876 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4171 4774 5945 1937
Size: 2234 603 1171 4594
Fragments arranged by size:
4594 2234 1171 603
Acc65I G'GTAC_C
Cuts at: 5923 5923
Size: 8602
AclI AA'CG_TT
Cuts at: 1563 3878 6729 7102 1563
Size: 2315 2851 373 3063
Fragments arranged by size:
3063 2851 2315 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 3298 3786 4200 5321 5367 5549 6665
Size: 1103 563 488 414 1121 46 182 1116
Cuts at: 6665 8049 8317 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1121 1116 1103 563 488 414
268 182 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8405
AflIII A'CryG_T
Cuts at: 1203 3589 3927 7501 8478 1203
Size: 2386 338 3574 977 1327
Fragments arranged by size:
3574 2386 1327 977 338
AgeI A'CCGG_T
Cuts at: 7433 7433
Size: 8602
AhdI GACnn_n'nnGTC
Cuts at: 2455 5152 7331 2455
Size: 2697 2179 3726
Fragments arranged by size:
3726 2697 2179
AleI CACnn'nnGTG
Cuts at: 1166 2966 5073 1166
Size: 1800 2107 4695
Fragments arranged by size:
4695 2107 1800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4902 5541 1184
Size: 3718 639 4245
Fragments arranged by size:
4245 3718 639
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 2021
Size: 32 8570
AlwNI CAG_nnn'CTG
Cuts at: 2713 3227 4798 6345 7917 2713
Size: 514 1571 1547 1572 3398
Fragments arranged by size:
3398 1572 1571 1547 514
ApaLI G'TGCA_C
Cuts at: 3593 6659 7815 3593
Size: 3066 1156 4380
Fragments arranged by size:
4380 3066 1156
ApoI r'AATT_y
Cuts at: 1493 3145 3967 4162 4785 5102 5911 6137 6289
Size: 1652 822 195 623 317 809 226 152
Cuts at: 6289 8398 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1652 822 809 623 317 226
195 152
AseI AT'TA_AT
Cuts at: 160 1032 7156 160
Size: 872 6124 1606
Fragments arranged by size:
6124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8602
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2087 2210 2241 2308 2453 2783
Size: 73 114 30 123 31 67 145 330
Cuts at: 2783 2880 5927 1870
Size: 97 3047 4545
Fragments arranged by size:
4545 3047 330 145 123 114 97 73
67 31 30
BamHI G'GATC_C
Cuts at: 5932 6299 5932
Size: 367 8235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 2779 2822 5923 7378
Size: 325 217 627 443 549 43 3101 1455
Cuts at: 7378 618
Size: 1842
Fragments arranged by size:
3101 1842 1455 627 549 443 325 217
43
BanII G_rGCy'C
Cuts at: 1391 2058 3988 4022 5921 1391
Size: 667 1930 34 1899 4072
Fragments arranged by size:
4072 1930 1899 667 34
BbeI G_GCGC'C
Cuts at: 2234 2234
Size: 8602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3590 3922 4082 4091 928
Size: 971 1691 332 160 9 5439
Fragments arranged by size:
5439 1691 971 332 160 9
BbvCI CC'TCA_GC
Cuts at: 3865 4010 4369 3865
Size: 145 359 8098
Fragments arranged by size:
8098 359 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2921 2955 6793 6827 1903
Size: 34 984 34 3838 34 3678
Fragments arranged by size:
3838 3678 984 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5018 6494 7704 5018
Size: 1476 1210 5916
Fragments arranged by size:
5916 1476 1210
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 7720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7213 136
Size: 107 122 71 631 605 5541 1525
Fragments arranged by size:
5541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5209 8597 5209
Size: 3388 5214
BlpI GC'TnA_GC
Cuts at: 2076 2261 4890 6019 2076
Size: 185 2629 1129 4659
Fragments arranged by size:
4659 2629 1129 185
Bme1580I G_kGCm'C
Cuts at: 1790 2318 2784 3597 3925 6663 7819 1790
Size: 528 466 813 328 2738 1156 2573
Fragments arranged by size:
2738 2573 1156 813 528 466 328
BmgBI CAC'GTC
Cuts at: 1318 3040 1318
Size: 1722 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3027 5241 6333
Size: 416 53 83 399 24 1610 2214 1092
Cuts at: 6333 7291 442
Size: 958 1753
Fragments arranged by size:
2214 1753 1610 1092 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4631
Size: 8602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 3204 4006 4069 4601 5073 5633 7262
Size: 833 1132 802 63 532 472 560 1629
Cuts at: 7262 1239
Size: 2579
Fragments arranged by size:
2579 1629 1132 833 802 560 532 472
63
Bpu10I CC'TnA_GC
Cuts at: 2895 3865 3987 4010 4369 2895
Size: 970 122 23 359 7128
Fragments arranged by size:
7128 970 359 122 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2644 3715 6727 7592 7890 2644
Size: 1071 3012 865 298 3356
Fragments arranged by size:
3356 3012 1071 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2949 3419 5723 5847 7265 882
Size: 617 1450 470 2304 124 1418 2219
Fragments arranged by size:
2304 2219 1450 1418 617 470 124
BsaAI yAC'GTr
Cuts at: 493 1204 3592 6393 493
Size: 711 2388 2801 2702
Fragments arranged by size:
2801 2702 2388 711
BsaBI GATnn'nnATC
Cuts at: 8596 8596
Size: 8602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2231 2586 6409
Size: 53 83 186 936 534 164 355 3823
Cuts at: 6409 6791 7426 275
Size: 382 635 1451
Fragments arranged by size:
3823 1451 936 635 534 382 355 186
164 83 53
BsaWI w'CCGG_w
Cuts at: 2740 5751 5802 7035 7433 7707 7854 8265 2740
Size: 3011 51 1233 398 274 147 411 3077
Fragments arranged by size:
3077 3011 1233 411 398 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2927 2957 1178
Size: 30 165 30 1524 30 6823
Fragments arranged by size:
6823 1524 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2825 3151 4293 4457 4537 4794 1186
Size: 1639 326 1142 164 80 257 4994
Fragments arranged by size:
4994 1639 1142 326 257 164 80
BseYI C'CCAG_C
Cuts at: 2015 2300 2495 2567 3003 7805 8301 2015
Size: 285 195 72 436 4802 496 2316
Fragments arranged by size:
4802 2316 496 436 285 195 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2681 2971 4054 5051 1163
Size: 573 945 290 1083 997 4714
Fragments arranged by size:
4714 1083 997 945 573 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6052 6813 7841 1540
Size: 275 186 4051 761 1028 2301
Fragments arranged by size:
4051 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2367 3597 4022 6663 6748 7819 2262
Size: 105 1230 425 2641 85 1071 3045
Fragments arranged by size:
3045 2641 1230 1071 425 105 85
BsmI GAATG_Cn'
Cuts at: 2022 6113 6206 2022
Size: 4091 93 4418
Fragments arranged by size:
4418 4091 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 2074
Size: 8602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2262 2318 2367 2784 3597 3925
Size: 399 268 204 56 49 417 813 328
Cuts at: 3925 3988 4022 5921 6663 6748 7819 1391
Size: 63 34 1899 742 85 1071 2174
Fragments arranged by size:
2174 1899 1071 813 742 417 399 328
268 204 85 63 56 49 34
BspEI T'CCGG_A
Cuts at: 2740 5751 5802 8265 2740
Size: 3011 51 2463 3077
Fragments arranged by size:
3077 3011 2463 51
BspHI T'CATG_A
Cuts at: 5194 6491 8221 5194
Size: 1297 1730 5575
Fragments arranged by size:
5575 1730 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2854 4268 5957 844
Size: 2010 1414 1689 3489
Fragments arranged by size:
3489 2010 1689 1414
BsrBI CCG'CTC
Cuts at: 1394 1823 2899 6489 1394
Size: 429 1076 3590 3507
Fragments arranged by size:
3590 3507 1076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4273 4380 7097 7271 65
Size: 1183 3025 107 2717 174 1396
Fragments arranged by size:
3025 2717 1396 1183 174 107
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 2963 7246 7433 1700
Size: 110 423 730 4283 187 2869
Fragments arranged by size:
4283 2869 730 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 4062 96
Size: 3966 4636
BssHII G'CGCG_C
Cuts at: 1925 2265 1925
Size: 340 8262
BssSI C'ACGA_G
Cuts at: 2984 4907 6662 7674 2984
Size: 1923 1755 1012 3912
Fragments arranged by size:
3912 1923 1755 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3385 794
Size: 2591 6011
BstBI TT'CG_AA
Cuts at: 2610 3913 4567 7406 2610
Size: 1303 654 2839 3806
Fragments arranged by size:
3806 2839 1303 654
BstEII G'GTnAC_C
Cuts at: 1184 2632 1184
Size: 1448 7154
BstYI r'GATC_y
Cuts at: 1972 2187 2718 3698 4311 5209 5932 6299 6684
Size: 215 531 980 613 898 723 367 385
Cuts at: 6684 6701 8153 8239 8251 8597 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 980 898 723 613 531 385
367 346 215 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3557 4337 3557
Size: 780 7822
BtgI C'CryG_G
Cuts at: 513 1065 2004 4437 5817 513
Size: 552 939 2433 1380 3298
Fragments arranged by size:
3298 2433 1380 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7863
Fragments arranged by size:
7863 683 56
BtsI GCAGTG_nn'
Cuts at: 2962 3661 4128 4209 4884 6118 6912 6932 2962
Size: 699 467 81 675 1234 794 20 4632
Fragments arranged by size:
4632 1234 794 699 675 467 81 20
ClaI AT'CG_AT
Cuts at: 1554 2670 1554
Size: 1116 7486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8567
DraI TTT'AAA
Cuts at: 3151 5906 3151
Size: 2755 5847
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7609 809
Size: 507 6293 1802
Fragments arranged by size:
6293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2328 2544
Size: 54 1425 50 275 18 351 147 216
Cuts at: 2544 6049 6938 8567 8
Size: 3505 889 1629 43
Fragments arranged by size:
3505 1629 1425 889 351 275 216 147
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6049 1537
Size: 275 4237 4090
Fragments arranged by size:
4237 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3083 3882 4752 5073 6532 1353
Size: 1730 799 870 321 1459 3423
Fragments arranged by size:
3423 1730 1459 870 799 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7189 7563 7709 387
Size: 1064 138 5600 374 146 1280
Fragments arranged by size:
5600 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4020 4020
Size: 8602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2735 3204 3298 3786 4006 4069
Size: 393 440 663 469 94 488 220 63
Cuts at: 4069 4200 4601 5073 5321 5367 5549 5633 6665
Size: 131 401 472 248 46 182 84 1032
Cuts at: 6665 7262 8049 8317 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1032 787 663 597 488 472 469
440 401 393 268 248 220 182 131
94 84 63 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2807 2432
Size: 375 8227
EcoO109I rG'GnC_Cy
Cuts at: 2304 2653 2755 2787 3608 4248 2304
Size: 349 102 32 821 640 6658
Fragments arranged by size:
6658 821 640 349 102 32
EcoRI G'AATT_C
Cuts at: 4162 5911 6289 4162
Size: 1749 378 6475
Fragments arranged by size:
6475 1749 378
EcoRV GAT'ATC
Cuts at: 3157 3157
Size: 8602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3718 5411 3718
Size: 1693 6909
FspI TGC'GCA
Cuts at: 7108 7108
Size: 8602
HaeII r_GCGC'y
Cuts at: 1678 2234 2301 2713 7749 1678
Size: 556 67 412 5036 2531
Fragments arranged by size:
5036 2531 556 412 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 2441 2473 2863
Size: 32 135 32 663 32 174 32 390
Cuts at: 2863 2895 2927 2959 3110 3142 3146 3178 3397
Size: 32 32 32 151 32 4 32 219
Cuts at: 3397 3429 3884 3916 4701 4733 5138 5170 7243
Size: 32 455 32 785 32 405 32 2073
Cuts at: 7243 7275 7317 7349 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2073 785 663 455 405 390 219
174 151 135 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4775 5946 6192 669
Size: 988 281 2837 1171 246 3079
Fragments arranged by size:
3079 2837 1171 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4150 748
Size: 3402 5200
HpaI GTT'AAC
Cuts at: 6192 6192
Size: 8602
KasI G'GCGC_C
Cuts at: 2230 2230
Size: 8602
KpnI G_GTAC'C
Cuts at: 5927 5927
Size: 8602
MfeI C'AATT_G
Cuts at: 5833 6201 5833
Size: 368 8234
MluI A'CGCG_T
Cuts at: 8478 8478
Size: 8602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3813 3921 4457 7716 7900 1305
Size: 2508 108 536 3259 184 2007
Fragments arranged by size:
3259 2508 2007 536 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6780
Fragments arranged by size:
6780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2966 5073 5529 6560 6919 7078 518
Size: 648 1800 2107 456 1031 359 159 2042
Fragments arranged by size:
2107 2042 1800 1031 648 456 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 3565 4453 4972 5179 5819 6018 6695
Size: 413 1546 888 519 207 640 199 677
Cuts at: 6695 7843 8088 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1546 1148 888 677 640 519 413
245 207 199
NaeI GCC'GGC
Cuts at: 2235 2235
Size: 8602
NarI GG'CG_CC
Cuts at: 2231 2231
Size: 8602
NcoI C'CATG_G
Cuts at: 513 1065 2004 4437 513
Size: 552 939 2433 4678
Fragments arranged by size:
4678 2433 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8602
NgoMIV G'CCGG_C
Cuts at: 2233 2233
Size: 8602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8602
NotI GC'GGCC_GC
Cuts at: 6049 6049
Size: 8602
NspI r_CATG'y
Cuts at: 1132 1229 3188 4063 4662 4901 5528 5960 7505
Size: 97 1959 875 599 239 627 432 1545
Cuts at: 7505 1132
Size: 2229
Fragments arranged by size:
2229 1959 1545 875 627 599 432 239
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8602
PciI A'CATG_T
Cuts at: 7501 7501
Size: 8602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3227 5695 1108
Size: 2119 2468 4015
Fragments arranged by size:
4015 2468 2119
PmeI GTTT'AAAC
Cuts at: 5906 5906
Size: 8602
PmlI CAC'GTG
Cuts at: 3592 3592
Size: 8602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6671 6703 8184 8216 6671
Size: 32 1481 32 7057
Fragments arranged by size:
7057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2755 2787 4248 2755
Size: 32 1461 7109
Fragments arranged by size:
7109 1461 32
PshAI GACnn'nnGTC
Cuts at: 1613 4972 1613
Size: 3359 5243
PsiI TTA'TAA
Cuts at: 5399 6172 5399
Size: 773 7829
PstI C_TGCA'G
Cuts at: 830 3336 4265 4709 5163 5954 830
Size: 2506 929 444 454 791 3478
Fragments arranged by size:
3478 2506 929 791 454 444
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8602
PvuII CAG'CTG
Cuts at: 2019 3565 4972 5179 2019
Size: 1546 1407 207 5442
Fragments arranged by size:
5442 1546 1407 207
RsrII CG'GwC_CG
Cuts at: 2738 2738
Size: 8602
SacI G_AGCT'C
Cuts at: 4022 4022
Size: 8602
SacII CC_GC'GG
Cuts at: 5820 5820
Size: 8602
SalI G'TCGA_C
Cuts at: 1936 4773 5944 1936
Size: 2837 1171 4594
Fragments arranged by size:
4594 2837 1171
SanDI GG'GwC_CC
Cuts at: 2787 2787
Size: 8602
SapI GCTCTTCn'nnn_
Cuts at: 3083 5073 3083
Size: 1990 6612
SbfI CC_TGCA'GG
Cuts at: 5954 5954
Size: 8602
ScaI AGT'ACT
Cuts at: 6850 8562 6850
Size: 1712 6890
SfcI C'TryA_G
Cuts at: 826 1045 3332 4261 4705 5159 5552 5950 7085
Size: 219 2287 929 444 454 393 398 1135
Cuts at: 7085 7766 7957 826
Size: 681 191 1471
Fragments arranged by size:
2287 1471 1135 929 681 454 444 398
393 219 191
SfoI GGC'GCC
Cuts at: 2232 2232
Size: 8602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8602
SgrAI Cr'CCGG_yG
Cuts at: 1700 2233 2963 1700
Size: 533 730 7339
Fragments arranged by size:
7339 730 533
SmaI CCC'GGG
Cuts at: 1872 2089 2785 5929 1872
Size: 217 696 3144 4545
Fragments arranged by size:
4545 3144 696 217
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2659 3730 6706 7607 7869 820
Size: 197 926 716 1071 2976 901 262 1553
Fragments arranged by size:
2976 1553 1071 926 901 716 262 197
SnaBI TAC'GTA
Cuts at: 493 6393 493
Size: 5900 2702
SpeI A'CTAG_T
Cuts at: 152 2318 152
Size: 2166 6436
SphI G_CATG'C
Cuts at: 1132 4901 5528 5960 1132
Size: 3769 627 432 3774
Fragments arranged by size:
3774 3769 627 432
SspI AAT'ATT
Cuts at: 5 52 5425 6526 5
Size: 47 5373 1101 2081
Fragments arranged by size:
5373 2081 1101 47
StuI AGG'CCT
Cuts at: 1517 3311 3368 5693 1517
Size: 1794 57 2325 4426
Fragments arranged by size:
4426 2325 1794 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2722 2836 4437 5694 6041 513
Size: 552 939 718 114 1601 1257 347 3074
Fragments arranged by size:
3074 1601 1257 939 718 552 347 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 2931 3046 3954 6629 6646 6799
Size: 1255 1266 85 115 908 2675 17 153
Cuts at: 6799 6984 7429 325
Size: 185 445 1498
Fragments arranged by size:
2675 1498 1266 1255 908 445 185 153
115 85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 3629 4062
Size: 274 80 33 51 1443 642 1010 433
Cuts at: 4062 4951 5136 6848 8560 96
Size: 889 185 1712 1712 138
Fragments arranged by size:
1712 1712 1443 1010 889 642 433 274
185 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8562 8594 8562
Size: 32 8570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8602
XbaI T'CTAG_A
Cuts at: 5938 5938
Size: 8602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3274 3274
Size: 8602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8602
XmaI C'CCGG_G
Cuts at: 1870 2087 2783 5927 1870
Size: 217 696 3144 4545
Fragments arranged by size:
4545 3144 696 217
XmnI GAAnn'nnTTC
Cuts at: 3498 4737 4761 6731 3498
Size: 1239 24 1970 5369
Fragments arranged by size:
5369 1970 1239 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6410 276
Size: 53 83 186 936 4876 2468
Fragments arranged by size:
4876 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I DraIII FseI FspAI NruI NsiI PacI
PfoI PspOMI PspXI PsrI SexAI SfiI SrfI SwaI