Restriction map for
pFN21AA0602
(Circular) (Six-base) MAPSORT of: pFN21AA0602.seq Check: 5276 from: 1 to: 7417
pFN21AA0602 7417 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4282 4282
Size: 7417
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2735 3537 3588 4632
Size: 53 83 186 936 1199 802 51 1044
Cuts at: 4632 5227 278
Size: 595 2468
Fragments arranged by size:
2468 1199 1044 936 802 595 186 83
53 51
AccI GT'mk_AC
Cuts at: 1937 4760 1937
Size: 2823 4594
Acc65I G'GTAC_C
Cuts at: 3804 4738 3804
Size: 934 6483
AclI AA'CG_TT
Cuts at: 1563 5544 5917 1563
Size: 3981 373 3063
Fragments arranged by size:
3981 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2321 2466 3583 5480 6864 7132 1632
Size: 530 159 145 1117 1897 1384 268 1917
Fragments arranged by size:
1917 1897 1384 1117 530 268 159 145
AfeI AGC'GCT
Cuts at: 2363 2775 3377 2363
Size: 412 602 6403
Fragments arranged by size:
6403 602 412
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7220
AflIII A'CryG_T
Cuts at: 1203 6316 7293 1203
Size: 5113 977 1327
Fragments arranged by size:
5113 1327 977
AgeI A'CCGG_T
Cuts at: 6248 6248
Size: 7417
AhdI GACnn_n'nnGTC
Cuts at: 3916 4048 4525 6146 3916
Size: 132 477 1621 5187
Fragments arranged by size:
5187 1621 477 132
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2844 1184
Size: 1660 5757
AlwNI CAG_nnn'CTG
Cuts at: 2461 3850 5160 6732 2461
Size: 1389 1310 1572 3146
Fragments arranged by size:
3146 1572 1389 1310
ApaLI G'TGCA_C
Cuts at: 3565 4381 5474 6630 3565
Size: 816 1093 1156 4352
Fragments arranged by size:
4352 1156 1093 816
ApoI r'AATT_y
Cuts at: 1493 4726 4952 5104 7213 1493
Size: 3233 226 152 2109 1697
Fragments arranged by size:
3233 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2037 2037
Size: 7417
AseI AT'TA_AT
Cuts at: 160 1032 5971 160
Size: 872 4939 1606
Fragments arranged by size:
4939 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
AvaI C'yCGr_G
Cuts at: 1870 1943 2843 2989 3065 3142 3176 4147 4742
Size: 73 900 146 76 77 34 971 595
Cuts at: 4742 1870
Size: 4545
Fragments arranged by size:
4545 971 900 595 146 77 76 73
34
AvrII C'CTAG_G
Cuts at: 3808 3808
Size: 7417
BamHI G'GATC_C
Cuts at: 4747 5114 4747
Size: 367 7050
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3804 4271 4738 6193 618
Size: 325 217 627 2017 467 467 1455 1842
Fragments arranged by size:
2017 1842 1455 627 467 467 325 217
BanII G_rGCy'C
Cuts at: 1391 2216 2411 2996 3045 3788 4154 4736 1391
Size: 825 195 585 49 743 366 582 4072
Fragments arranged by size:
4072 825 743 585 582 366 195 49
BbeI G_GCGC'C
Cuts at: 4275 4275
Size: 7417
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 2556 3077 3434 928
Size: 971 250 407 521 357 4911
Fragments arranged by size:
4911 971 521 407 357 250
BbvCI CC'TCA_GC
Cuts at: 2424 2939 3183 2424
Size: 515 244 6658
Fragments arranged by size:
6658 515 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5608 5642 1903
Size: 34 3671 34 3678
Fragments arranged by size:
3678 3671 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3612 5309 6519 2313
Size: 1299 1697 1210 3211
Fragments arranged by size:
3211 1697 1299 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2034 3300
Size: 107 122 71 631 605 353 9 1266
Cuts at: 3300 4202 6028 136
Size: 902 1826 1525
Fragments arranged by size:
1826 1525 1266 902 631 605 353 122
107 71 9
BglII A'GATC_T
Cuts at: 2508 7412 2508
Size: 4904 2513
BlpI GC'TnA_GC
Cuts at: 2462 4834 2462
Size: 2372 5045
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2121 3066 3569 3958 4385 5478 6634
Size: 277 54 945 503 389 427 1093 1156
Cuts at: 6634 1790
Size: 2573
Fragments arranged by size:
2573 1156 1093 945 503 427 389 277
54
BmgBI CAC'GTC
Cuts at: 1318 2689 3061 4522 1318
Size: 1371 372 1461 4213
Fragments arranged by size:
4213 1461 1371 372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 3791 5148
Size: 416 53 83 399 24 833 1541 1357
Cuts at: 5148 6106 442
Size: 958 1753
Fragments arranged by size:
1753 1541 1357 958 833 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2200 2638 4126 2039
Size: 161 438 1488 5330
Fragments arranged by size:
5330 1488 438 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 2189 3877 3899 4382 6077 1239
Size: 847 103 1688 22 483 1695 2579
Fragments arranged by size:
2579 1695 1688 847 483 103 22
Bpu10I CC'TnA_GC
Cuts at: 2424 2939 3183 4034 2424
Size: 515 244 851 5807
Fragments arranged by size:
5807 851 515 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4361 5542 6407 6705 4361
Size: 1181 865 298 5073
Fragments arranged by size:
5073 1181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3855 6080 882
Size: 617 2356 2225 2219
Fragments arranged by size:
2356 2225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 4669 5208 493
Size: 711 2366 1099 539 2702
Fragments arranged by size:
2702 2366 1099 711 539
BsaBI GATnn'nnATC
Cuts at: 2513 7411 2513
Size: 4898 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2649 2732 3387 3534
Size: 53 83 186 936 1116 83 655 147
Cuts at: 3534 3585 4272 4338 4629 5224 5606 6241 275
Size: 51 687 66 291 595 382 635 1451
Fragments arranged by size:
1451 1116 936 687 655 635 595 382
291 186 147 83 83 66 53 51
BsaWI w'CCGG_w
Cuts at: 4389 5850 6248 6522 6669 7080 4389
Size: 1461 398 274 147 411 4726
Fragments arranged by size:
4726 1461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3559 3589 3713 3743 3797
Size: 30 165 30 2156 30 124 30 54
Cuts at: 3797 3827 1178
Size: 30 4768
Fragments arranged by size:
4768 2156 165 124 54 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2614 2618 2621 2867 4156 4213 1186
Size: 1428 4 3 246 1289 57 4390
Fragments arranged by size:
4390 1428 1289 246 57 4 3
BseYI C'CCAG_C
Cuts at: 2108 2897 3224 3645 4256 4415 6620 7116 2108
Size: 789 327 421 611 159 2205 496 2409
Fragments arranged by size:
2409 2205 789 611 496 421 327 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 2675 3428 4552 4642 1163
Size: 573 413 526 753 1124 90 3938
Fragments arranged by size:
3938 1124 753 573 526 413 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2505 4867 5628 6656 1540
Size: 275 186 504 2362 761 1028 2301
Fragments arranged by size:
2362 2301 1028 761 504 275 186
BsiHKAI G_wGCw'C
Cuts at: 2838 2895 2996 3153 3311 3569 3575 4385 5478
Size: 57 101 157 158 258 6 810 1093
Cuts at: 5478 5563 6634 2838
Size: 85 1071 3621
Fragments arranged by size:
3621 1093 1071 810 258 158 157 101
85 57 6
BsmI GAATG_Cn'
Cuts at: 4928 5021 4928
Size: 93 7324
BsmBI CGTCTCn'nnnn_
Cuts at: 3616 3616
Size: 7417
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2121 2216 2411 2838 2895 2996
Size: 399 277 54 95 195 427 57 101
Cuts at: 2996 3045 3066 3153 3311 3569 3575 3788 3958
Size: 49 21 87 158 258 6 213 170
Cuts at: 3958 4154 4385 4736 5478 5563 6634 1391
Size: 196 231 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 351 277 258
231 213 196 195 170 158 101 95
87 85 57 54 49 21 6
BspEI T'CCGG_A
Cuts at: 7080 7080
Size: 7417
BspHI T'CATG_A
Cuts at: 5306 7036 5306
Size: 1730 5687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4282 4362 4772 844
Size: 3438 80 410 3489
Fragments arranged by size:
3489 3438 410 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3325 3352 4239 5304 1394
Size: 429 1502 27 887 1065 3507
Fragments arranged by size:
3507 1502 1065 887 429 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 5912 6086 65
Size: 1183 4664 174 1396
Fragments arranged by size:
4664 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2567 4557 6061 6248 1700
Size: 110 757 1990 1504 187 2869
Fragments arranged by size:
2869 1990 1504 757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7417
BssHII G'CGCG_C
Cuts at: 1925 2037 2046 1925
Size: 112 9 7296
Fragments arranged by size:
7296 112 9
BssSI C'ACGA_G
Cuts at: 5477 6489 5477
Size: 1012 6405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4190 4319 4591 794
Size: 3396 129 272 3620
Fragments arranged by size:
3620 3396 272 129
BstBI TT'CG_AA
Cuts at: 6221 6221
Size: 7417
BstEII G'GTnAC_C
Cuts at: 1184 4462 1184
Size: 3278 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 3364 4459 2419
Size: 945 1095 5377
Fragments arranged by size:
5377 1095 945
BstYI r'GATC_y
Cuts at: 1972 2508 3417 4747 5114 5499 5516 6968 7054
Size: 536 909 1330 367 385 17 1452 86
Cuts at: 7054 7066 7412 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1330 909 536 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3165 3165
Size: 7417
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2403 2586 3558 3672 3795
Size: 552 939 183 216 183 972 114 123
Cuts at: 3795 3816 4458 513
Size: 21 642 3472
Fragments arranged by size:
3472 972 939 642 552 216 183 183
123 114 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3922 3955 487
Size: 683 56 2696 33 3949
Fragments arranged by size:
3949 2696 683 56 33
BtsI GCAGTG_nn'
Cuts at: 4569 4661 4933 5727 5747 4569
Size: 92 272 794 20 6239
Fragments arranged by size:
6239 794 272 92 20
ClaI AT'CG_AT
Cuts at: 1554 2717 1554
Size: 1163 6254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7382
DraI TTT'AAA
Cuts at: 4721 4721
Size: 7417
DraIII CAC_nnn'GTG
Cuts at: 3796 3796
Size: 7417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2933 4358 6424 809
Size: 507 1617 1425 2066 1802
Fragments arranged by size:
2066 1802 1617 1425 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2400 2452
Size: 54 1425 50 275 18 177 393 52
Cuts at: 2452 2502 2963 3292 3798 4113 4194 4647 4864
Size: 50 461 329 506 315 81 453 217
Cuts at: 4864 5753 7382 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 506 461 453 393 329
315 275 217 177 81 54 52 50
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2502 4864 1537
Size: 275 690 2362 4090
Fragments arranged by size:
4090 2362 690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3995 4041 4325 5347 1353
Size: 959 1683 46 284 1022 3423
Fragments arranged by size:
3423 1683 1022 959 284 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6004 6378 6524 387
Size: 1064 138 4415 374 146 1280
Fragments arranged by size:
4415 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2994 2994
Size: 7417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2162 2189 2321 2466 3583 3877
Size: 393 454 76 27 132 145 1117 294
Cuts at: 3877 3899 4382 5480 6077 6864 7132 1239
Size: 22 483 1098 597 787 268 1524
Fragments arranged by size:
1524 1117 1098 787 597 483 454 393
294 268 145 132 76 27 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3268 3268
Size: 7417
EcoO109I rG'GnC_Cy
Cuts at: 2488 2806 2824 2857 3847 4351 4600 2488
Size: 318 18 33 990 504 249 5305
Fragments arranged by size:
5305 990 504 318 249 33 18
EcoRI G'AATT_C
Cuts at: 4726 5104 4726
Size: 378 7039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4729 4729
Size: 7417
FseI GG_CCGG'CC
Cuts at: 2571 2571
Size: 7417
FspI TGC'GCA
Cuts at: 5923 5923
Size: 7417
HaeII r_GCGC'y
Cuts at: 1678 2365 2777 3027 3379 4275 6564 1678
Size: 687 412 250 352 896 2289 2531
Fragments arranged by size:
2531 2289 896 687 412 352 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 3095 3127 3713
Size: 32 135 32 1010 32 481 32 586
Cuts at: 3713 3745 3902 3934 4034 4066 4511 4543 6058
Size: 32 157 32 100 32 445 32 1515
Cuts at: 6058 6090 6132 6164 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1515 1010 586 481 445 157 135
100 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3499 4761 5007 669
Size: 988 281 1561 1262 246 3079
Fragments arranged by size:
3079 1561 1262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2333 748
Size: 1585 5832
HpaI GTT'AAC
Cuts at: 5007 5007
Size: 7417
KasI G'GCGC_C
Cuts at: 4271 4271
Size: 7417
KpnI G_GTAC'C
Cuts at: 3808 4742 3808
Size: 934 6483
MfeI C'AATT_G
Cuts at: 5016 5016
Size: 7417
MluI A'CGCG_T
Cuts at: 7293 7293
Size: 7417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2823 3555 4072 6531 6715 1305
Size: 1518 732 517 2459 184 2007
Fragments arranged by size:
2459 2007 1518 732 517 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2454 3800 10
Size: 54 1425 343 622 1346 3627
Fragments arranged by size:
3627 1425 1346 622 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2417 4451 4457 5375 5734 5893
Size: 648 894 357 2034 6 918 359 159
Cuts at: 5893 518
Size: 2042
Fragments arranged by size:
2042 2034 918 894 648 359 357 159
6
MspA1I CmG'CkG
Cuts at: 1606 2112 2405 2710 2907 3228 3412 3727 4422
Size: 506 293 305 197 321 184 315 695
Cuts at: 4422 4645 4833 5510 6658 6903 1606
Size: 223 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 695 677 506 321 315 305
293 245 223 197 188 184
NaeI GCC'GGC
Cuts at: 2569 4559 2569
Size: 1990 5427
NarI GG'CG_CC
Cuts at: 4272 4272
Size: 7417
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5926
Fragments arranged by size:
5926 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7417
NgoMIV G'CCGG_C
Cuts at: 2567 4557 2567
Size: 1990 5427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7417
NotI GC'GGCC_GC
Cuts at: 4864 4864
Size: 7417
NspI r_CATG'y
Cuts at: 1132 1229 3763 4594 4775 6320 1132
Size: 97 2534 831 181 1545 2229
Fragments arranged by size:
2534 2229 1545 831 181 97
PasI CC'CwG_GG
Cuts at: 1348 3082 1348
Size: 1734 5683
PciI A'CATG_T
Cuts at: 6316 6316
Size: 7417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3796 3850 1108
Size: 2688 54 4675
Fragments arranged by size:
4675 2688 54
PfoI T'CCnGG_A
Cuts at: 2808 3843 2808
Size: 1035 6382
PmeI GTTT'AAAC
Cuts at: 4721 4721
Size: 7417
PmlI CAC'GTG
Cuts at: 3570 4669 3570
Size: 1099 6318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5486 5518 6999 7031 5486
Size: 32 1481 32 5872
Fragments arranged by size:
5872 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2806 2824 2857 3847 4351 4600 2806
Size: 18 33 990 504 249 5623
Fragments arranged by size:
5623 990 504 249 33 18
PshAI GACnn'nnGTC
Cuts at: 1613 4460 1613
Size: 2847 4570
PsiI TTA'TAA
Cuts at: 4987 4987
Size: 7417
PspXI vC'TCGA_Gb
Cuts at: 2989 2989
Size: 7417
PstI C_TGCA'G
Cuts at: 830 2352 2474 3417 3543 4293 4435 4769 830
Size: 1522 122 943 126 750 142 334 3478
Fragments arranged by size:
3478 1522 943 750 334 142 126 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7417
PvuII CAG'CTG
Cuts at: 2112 3412 3727 4645 2112
Size: 1300 315 918 4884
Fragments arranged by size:
4884 1300 918 315
SacI G_AGCT'C
Cuts at: 2996 2996
Size: 7417
SacII CC_GC'GG
Cuts at: 2406 2406
Size: 7417
SalI G'TCGA_C
Cuts at: 1936 4759 1936
Size: 2823 4594
SbfI CC_TGCA'GG
Cuts at: 4769 4769
Size: 7417
ScaI AGT'ACT
Cuts at: 5665 7377 5665
Size: 1712 5705
SexAI A'CCwGG_T
Cuts at: 3269 3269
Size: 7417
SfcI C'TryA_G
Cuts at: 826 1045 2348 2470 3401 3413 3539 4289 4431
Size: 219 1303 122 931 12 126 750 142
Cuts at: 4431 4765 5900 6581 6772 826
Size: 334 1135 681 191 1471
Fragments arranged by size:
1471 1303 1135 931 750 681 334 219
191 142 126 122 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2025 3300 4202 2025
Size: 1275 902 5240
Fragments arranged by size:
5240 1275 902
SfoI GGC'GCC
Cuts at: 4273 4273
Size: 7417
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7417
SmaI CCC'GGG
Cuts at: 1872 4744 1872
Size: 2872 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2989 4376 5521 6422 6684 820
Size: 197 926 1046 1387 1145 901 262 1553
Fragments arranged by size:
1553 1387 1145 1046 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5208 493
Size: 4715 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7417
SphI G_CATG'C
Cuts at: 1132 4775 1132
Size: 3643 3774
SspI AAT'ATT
Cuts at: 5 52 5341 5
Size: 47 5289 2081
Fragments arranged by size:
5289 2081 47
StuI AGG'CCT
Cuts at: 1517 2979 3188 4205 4245 1517
Size: 1462 209 1017 40 4689
Fragments arranged by size:
4689 1462 1017 209 40
StyI C'CwwG_G
Cuts at: 513 1065 2004 2574 3808 4197 4467 4704 4856
Size: 552 939 570 1234 389 270 237 152
Cuts at: 4856 513
Size: 3074
Fragments arranged by size:
3074 1234 939 570 552 389 270 237
152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4225 5444 5461 5614 5799 6244 325
Size: 1255 2645 1219 17 153 185 445 1498
Fragments arranged by size:
2645 1498 1255 1219 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3933 5663 7375
Size: 274 80 33 51 1443 1956 1730 1712
Cuts at: 7375 96
Size: 138
Fragments arranged by size:
1956 1730 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3559 3591 7377 7409 3559
Size: 32 3786 32 3567
Fragments arranged by size:
3786 3567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2804 2933 3076 3107 3247 1316
Size: 1488 129 143 31 140 5486
Fragments arranged by size:
5486 1488 143 140 129 31
XbaI T'CTAG_A
Cuts at: 4753 4753
Size: 7417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2263 2089
Size: 174 7243
XhoI C'TCGA_G
Cuts at: 1943 2989 1943
Size: 1046 6371
XmaI C'CCGG_G
Cuts at: 1870 4742 1870
Size: 2872 4545
XmnI GAAnn'nnTTC
Cuts at: 2075 4676 5546 2075
Size: 2601 870 3946
Fragments arranged by size:
3946 2601 870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2733 3535 3586 4630
Size: 53 83 186 936 1199 802 51 1044
Cuts at: 4630 5225 276
Size: 595 2468
Fragments arranged by size:
2468 1199 1044 936 802 595 186 83
53 51
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PspOMI PsrI RsrII SanDI SapI
SrfI SwaI