Restriction map for
pFN21AA0600
(Circular) (Six-base) MAPSORT of: pFN21AA0600.seq Check: 9300 from: 1 to: 7816
pFN21AA0600 7816 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3913 4970 3913
Size: 1057 6759
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5626 278
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 5159 1937
Size: 1248 1974 4594
Fragments arranged by size:
4594 1974 1248
Acc65I G'GTAC_C
Cuts at: 5137 5137
Size: 7816
AclI AA'CG_TT
Cuts at: 1563 5943 6316 1563
Size: 4380 373 3063
Fragments arranged by size:
4380 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4491 4555 5879 7263 7531 1632
Size: 2859 64 1324 1384 268 1917
Fragments arranged by size:
2859 1917 1384 1324 268 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7619
AflIII A'CryG_T
Cuts at: 1203 3096 3430 6715 7692 1203
Size: 1893 334 3285 977 1327
Fragments arranged by size:
3285 1893 1327 977 334
AgeI A'CCGG_T
Cuts at: 6647 6647
Size: 7816
AhdI GACnn_n'nnGTC
Cuts at: 6545 6545
Size: 7816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4183 5124 1184
Size: 2999 941 3876
Fragments arranged by size:
3876 2999 941
AlwNI CAG_nnn'CTG
Cuts at: 2230 2269 2284 2425 2482 2494 2780 3223 3526
Size: 39 15 141 57 12 286 443 303
Cuts at: 3526 3535 3565 3910 3970 4460 5559 7131 2230
Size: 9 30 345 60 490 1099 1572 2915
Fragments arranged by size:
2915 1572 1099 490 443 345 303 286
141 60 57 39 30 15 12 9
ApaI G_GGCC'C
Cuts at: 2739 3855 2739
Size: 1116 6700
ApaLI G'TGCA_C
Cuts at: 5873 7029 5873
Size: 1156 6660
ApoI r'AATT_y
Cuts at: 1493 2570 5125 5351 5503 7612 1493
Size: 1077 2555 226 152 2109 1697
Fragments arranged by size:
2555 2109 1697 1077 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6370 160
Size: 872 5338 1606
Fragments arranged by size:
5338 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
AvaI C'yCGr_G
Cuts at: 1870 1943 3720 5061 5141 1870
Size: 73 1777 1341 80 4545
Fragments arranged by size:
4545 1777 1341 80 73
AvrII C'CTAG_G
Cuts at: 3124 3124
Size: 7816
BamHI G'GATC_C
Cuts at: 5146 5513 5146
Size: 367 7449
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2909 3934 4376 5137 6592
Size: 325 217 627 1122 1025 442 761 1455
Cuts at: 6592 618
Size: 1842
Fragments arranged by size:
1842 1455 1122 1025 761 627 442 325
217
BanII G_rGCy'C
Cuts at: 1391 2191 2201 2244 2598 2653 2739 3058 3317
Size: 800 10 43 354 55 86 319 259
Cuts at: 3317 3709 3731 3855 4019 4209 4468 5094 5135
Size: 392 22 124 164 190 259 626 41
Cuts at: 5135 1391
Size: 4072
Fragments arranged by size:
4072 800 626 392 354 319 259 259
190 164 124 86 55 43 41 22
10
BbeI G_GCGC'C
Cuts at: 3938 3938
Size: 7816
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3762 3786 928
Size: 971 1863 24 4958
Fragments arranged by size:
4958 1863 971 24
BbvCI CC'TCA_GC
Cuts at: 2558 3045 3843 3941 2558
Size: 487 798 98 6433
Fragments arranged by size:
6433 798 487 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3821 3855 6007 6041 1903
Size: 34 1884 34 2152 34 3678
Fragments arranged by size:
3678 2152 1884 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5708 6918 3872
Size: 1836 1210 4770
Fragments arranged by size:
4770 1836 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3288 4796 5042
Size: 107 122 71 631 605 1616 1508 246
Cuts at: 5042 6427 136
Size: 1385 1525
Fragments arranged by size:
1616 1525 1508 1385 631 605 246 122
107 71
BglII A'GATC_T
Cuts at: 7811 7811
Size: 7816
BlpI GC'TnA_GC
Cuts at: 2826 5233 2826
Size: 2407 5409
Bme1580I G_kGCm'C
Cuts at: 1790 2739 3855 3960 5877 7033 1790
Size: 949 1116 105 1917 1156 2573
Fragments arranged by size:
2573 1917 1156 1116 949 105
BmgBI CAC'GTC
Cuts at: 1318 3099 4552 1318
Size: 1781 1453 4582
Fragments arranged by size:
4582 1781 1453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 3976 4363
Size: 416 53 83 399 24 710 1849 387
Cuts at: 4363 5547 6505 442
Size: 1184 958 1753
Fragments arranged by size:
1849 1753 1184 958 710 416 399 387
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 3507 4153 2697
Size: 810 646 6360
Fragments arranged by size:
6360 810 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2489 2504 2549 3200 3320 3377 3587
Size: 1208 42 15 45 651 120 57 210
Cuts at: 3587 3782 4126 4196 4775 4942 6476 1239
Size: 195 344 70 579 167 1534 2579
Fragments arranged by size:
2579 1534 1208 651 579 344 210 195
167 120 70 57 45 42 15
Bpu10I CC'TnA_GC
Cuts at: 2558 3045 3482 3843 3941 2558
Size: 487 437 361 98 6433
Fragments arranged by size:
6433 487 437 361 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2339 4193 5941 6806 7104 2237
Size: 102 1854 1748 865 298 2949
Fragments arranged by size:
2949 1854 1748 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4979 6479 882
Size: 617 3480 1500 2219
Fragments arranged by size:
3480 2219 1500 617
BsaAI yAC'GTr
Cuts at: 493 1204 5607 493
Size: 711 4403 2702
Fragments arranged by size:
4403 2702 711
BsaBI GATnn'nnATC
Cuts at: 7810 7810
Size: 7816
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2705 2924 3935 5623
Size: 53 83 186 936 1172 219 1011 1688
Cuts at: 5623 6005 6640 275
Size: 382 635 1451
Fragments arranged by size:
1688 1451 1172 1011 936 635 382 219
186 83 53
BsaWI w'CCGG_w
Cuts at: 6249 6647 6921 7068 7479 6249
Size: 398 274 147 411 6586
Fragments arranged by size:
6586 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2824 2854 1178
Size: 30 165 30 1421 30 6140
Fragments arranged by size:
6140 1421 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3668 3677 3680 3779 3782 3806 3809 3812
Size: 2482 9 3 99 3 24 3 3
Cuts at: 3812 3839 3860 3878 4129 4653 5006 5102 1186
Size: 27 21 18 251 524 353 96 3900
Fragments arranged by size:
3900 2482 524 353 251 99 96 27
24 21 18 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2165 2643 2960 3286 3595 3694 3903 4380 7019
Size: 478 317 326 309 99 209 477 2639
Cuts at: 7019 7515 2165
Size: 496 2466
Fragments arranged by size:
2639 2466 496 478 477 326 317 309
209 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 4952 1163
Size: 573 327 2889 4027
Fragments arranged by size:
4027 2889 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5266 6027 7055 1540
Size: 275 186 3265 761 1028 2301
Fragments arranged by size:
3265 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 3263 3380 3749 4209 4413 5877 5962 7033
Size: 1019 117 369 460 204 1464 85 1071
Cuts at: 7033 2244
Size: 3027
Fragments arranged by size:
3027 1464 1071 1019 460 369 204 117
85
BsmI GAATG_Cn'
Cuts at: 5327 5420 5327
Size: 93 7723
BsmBI CGTCTCn'nnnn_
Cuts at: 2745 3105 4542 2745
Size: 360 1437 6019
Fragments arranged by size:
6019 1437 360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2201 2244 2598 2653 2739 3058
Size: 399 401 10 43 354 55 86 319
Cuts at: 3058 3263 3317 3380 3709 3731 3749 3855 3960
Size: 205 54 63 329 22 18 106 105
Cuts at: 3960 4019 4209 4413 4468 5094 5135 5877 5962
Size: 59 190 204 55 626 41 742 85
Cuts at: 5962 7033 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 626 401 399 354 329
319 205 204 190 106 105 86 85
63 59 55 55 54 43 41 22
18 10
BspEI T'CCGG_A
Cuts at: 7479 7479
Size: 7816
BspHI T'CATG_A
Cuts at: 3255 5705 7435 3255
Size: 2450 1730 3636
Fragments arranged by size:
3636 2450 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3913 4970 5171 844
Size: 3069 1057 201 3489
Fragments arranged by size:
3489 3069 1057 201
BsrBI CCG'CTC
Cuts at: 1394 1823 2691 2830 5703 1394
Size: 429 868 139 2873 3507
Fragments arranged by size:
3507 2873 868 429 139
BsrDI GCAATG_nn'
Cuts at: 65 1248 2535 3384 4378 4590 6311 6485 65
Size: 1183 1287 849 994 212 1721 174 1396
Fragments arranged by size:
1721 1396 1287 1183 994 849 212 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 3245 4956 5080 6460 6647 1700
Size: 110 300 1135 1711 124 1380 187 2869
Fragments arranged by size:
2869 1711 1380 1135 300 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7816
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7816
BssSI C'ACGA_G
Cuts at: 4598 5876 6888 4598
Size: 1278 1012 5526
Fragments arranged by size:
5526 1278 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 2269 2371 2398 2494 3346 3919 794
Size: 1436 39 102 27 96 852 573 4691
Fragments arranged by size:
4691 1436 852 573 102 96 39 27
BstBI TT'CG_AA
Cuts at: 6620 6620
Size: 7816
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7816
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 2173 4267 4373 2140
Size: 33 2094 106 5583
Fragments arranged by size:
5583 2094 106 33
BstYI r'GATC_y
Cuts at: 1972 3603 4905 5146 5513 5898 5915 7367 7453
Size: 1631 1302 241 367 385 17 1452 86
Cuts at: 7453 7465 7811 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1631 1452 1302 385 367 346 241
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3651 3651
Size: 7816
BtgI C'CryG_G
Cuts at: 513 1065 2066 2139 3138 3319 3400 3467 4266
Size: 552 1001 73 999 181 81 67 799
Cuts at: 4266 4366 5013 5094 513
Size: 100 647 81 3235
Fragments arranged by size:
3235 1001 999 799 647 552 181 100
81 81 73 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2974 4412 487
Size: 683 56 1748 1438 3891
Fragments arranged by size:
3891 1748 1438 683 56
BtsI GCAGTG_nn'
Cuts at: 2213 3165 4167 4884 5332 6126 6146 2213
Size: 952 1002 717 448 794 20 3883
Fragments arranged by size:
3883 1002 952 794 717 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7816
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7781
DraI TTT'AAA
Cuts at: 5120 5120
Size: 7816
DraIII CAC_nnn'GTG
Cuts at: 4122 4122
Size: 7816
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4031 6823 809
Size: 507 2715 2792 1802
Fragments arranged by size:
2792 2715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3435 3948 4699
Size: 54 1425 50 275 18 1605 513 751
Cuts at: 4699 4881 5263 6152 7781 8
Size: 182 382 889 1629 43
Fragments arranged by size:
1629 1605 1425 889 751 513 382 275
182 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5263 1537
Size: 275 3451 4090
Fragments arranged by size:
4090 3451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 2513 2882 4007 4049 4467 5746 1353
Size: 1104 56 369 1125 42 418 1279 3423
Fragments arranged by size:
3423 1279 1125 1104 418 369 56 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 6403 6777 6923 387
Size: 1064 138 3029 1785 374 146 1280
Fragments arranged by size:
3029 1785 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 4207 2242
Size: 1965 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2489 2504 2549 3200 3320 3377
Size: 393 815 42 15 45 651 120 57
Cuts at: 3377 3587 3782 4126 4196 4491 4555 4775 4942
Size: 210 195 344 70 295 64 220 167
Cuts at: 4942 5879 6476 7263 7531 1239
Size: 937 597 787 268 1524
Fragments arranged by size:
1524 937 815 787 651 597 393 344
295 268 220 210 195 167 120 70
64 57 45 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3764 4967 3227
Size: 537 1203 6076
Fragments arranged by size:
6076 1203 537
EcoO109I rG'GnC_Cy
Cuts at: 2736 3211 3523 3851 3967 4055 4339 2736
Size: 475 312 328 116 88 284 6213
Fragments arranged by size:
6213 475 328 312 284 116 88
EcoRI G'AATT_C
Cuts at: 2570 5125 5503 2570
Size: 2555 378 4883
Fragments arranged by size:
4883 2555 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4060 4060
Size: 7816
FspI TGC'GCA
Cuts at: 4154 6322 4154
Size: 2168 5648
FspAI rTGC'GCAy
Cuts at: 4154 4154
Size: 7816
HaeII r_GCGC'y
Cuts at: 1678 2252 3938 5013 6963 1678
Size: 574 1686 1075 1950 2531
Fragments arranged by size:
2531 1950 1686 1075 574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2023 2050 2055 2824
Size: 32 135 32 446 5 27 5 769
Cuts at: 2824 2856 3028 3060 4209 4241 6457 6489 6531
Size: 32 172 32 1149 32 2216 32 42
Cuts at: 6531 6563 1373
Size: 32 2626
Fragments arranged by size:
2626 2216 1149 769 446 172 135 42
32 32 32 32 32 32 32 27
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 5160 5406 669
Size: 988 281 1248 1974 246 3079
Fragments arranged by size:
3079 1974 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7816
HpaI GTT'AAC
Cuts at: 5406 5406
Size: 7816
KasI G'GCGC_C
Cuts at: 3934 3934
Size: 7816
KpnI G_GTAC'C
Cuts at: 5141 5141
Size: 7816
MfeI C'AATT_G
Cuts at: 5415 5415
Size: 7816
MluI A'CGCG_T
Cuts at: 7692 7692
Size: 7816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 6930 7114 1305
Size: 699 4926 184 2007
Fragments arranged by size:
4926 2007 699 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3437 3950 4701 4883 10
Size: 54 1425 343 1605 513 751 182 2943
Fragments arranged by size:
2943 1605 1425 751 513 343 182 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 3035 3324 3389 3443 4103 4160
Size: 648 924 945 289 65 54 660 57
Cuts at: 4160 4265 4371 4431 5774 6133 6292 518
Size: 105 106 60 1343 359 159 2042
Fragments arranged by size:
2042 1343 945 924 660 648 359 289
159 106 105 65 60 57 54
MspA1I CmG'CkG
Cuts at: 1606 2068 2269 2341 2437 2443 2455 2479 2494
Size: 462 201 72 96 6 12 24 15
Cuts at: 2494 3469 3505 3535 3595 3910 4717 4946 5232
Size: 975 36 30 60 315 807 229 286
Cuts at: 5232 5909 7057 7302 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 807 677 462 315 286
245 229 201 96 72 60 36 30
24 15 12 6
NaeI GCC'GGC
Cuts at: 5082 5082
Size: 7816
NarI GG'CG_CC
Cuts at: 3935 3935
Size: 7816
NcoI C'CATG_G
Cuts at: 513 1065 2139 4266 4366 5013 5094 513
Size: 552 1074 2127 100 647 81 3235
Fragments arranged by size:
3235 2127 1074 647 552 100 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7816
NgoMIV G'CCGG_C
Cuts at: 5080 5080
Size: 7816
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7816
NotI GC'GGCC_GC
Cuts at: 5263 5263
Size: 7816
NspI r_CATG'y
Cuts at: 1132 1229 3329 3347 3448 5174 6719 1132
Size: 97 2100 18 101 1726 1545 2229
Fragments arranged by size:
2229 2100 1726 1545 101 97 18
PasI CC'CwG_GG
Cuts at: 1348 2699 3970 4001 1348
Size: 1351 1271 31 5163
Fragments arranged by size:
5163 1351 1271 31
PciI A'CATG_T
Cuts at: 6715 6715
Size: 7816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2643 3970 4460 1108
Size: 1535 1327 490 4464
Fragments arranged by size:
4464 1535 1327 490
PfoI T'CCnGG_A
Cuts at: 2565 3564 2565
Size: 999 6817
PmeI GTTT'AAAC
Cuts at: 5120 5120
Size: 7816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5885 5917 7398 7430 5885
Size: 32 1481 32 6271
Fragments arranged by size:
6271 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4339 4339
Size: 7816
PshAI GACnn'nnGTC
Cuts at: 1613 4657 1613
Size: 3044 4772
PsiI TTA'TAA
Cuts at: 5386 5386
Size: 7816
PspOMI G'GGCC_C
Cuts at: 2735 3851 2735
Size: 1116 6700
PstI C_TGCA'G
Cuts at: 830 2235 2274 2346 3135 3342 3531 3915 4201
Size: 1405 39 72 789 207 189 384 286
Cuts at: 4201 4833 5168 830
Size: 632 335 3478
Fragments arranged by size:
3478 1405 789 632 384 335 286 207
189 72 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7816
PvuII CAG'CTG
Cuts at: 2269 2341 2494 3535 3595 4717 2269
Size: 72 153 1041 60 1122 5368
Fragments arranged by size:
5368 1122 1041 153 72 60
SacI G_AGCT'C
Cuts at: 2244 4209 2244
Size: 1965 5851
SacII CC_GC'GG
Cuts at: 2069 3470 2069
Size: 1401 6415
SalI G'TCGA_C
Cuts at: 1936 3184 5158 1936
Size: 1248 1974 4594
Fragments arranged by size:
4594 1974 1248
SapI GCTCTTCn'nnn_
Cuts at: 2457 2513 4007 2457
Size: 56 1494 6266
Fragments arranged by size:
6266 1494 56
SbfI CC_TGCA'GG
Cuts at: 5168 5168
Size: 7816
ScaI AGT'ACT
Cuts at: 6064 7776 6064
Size: 1712 6104
SfcI C'TryA_G
Cuts at: 826 1045 2231 2270 2342 3131 3338 3527 3590
Size: 219 1186 39 72 789 207 189 63
Cuts at: 3590 3911 4197 4290 4313 4829 5164 6299 6980
Size: 321 286 93 23 516 335 1135 681
Cuts at: 6980 7171 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 789 681 516 335 321
286 219 207 191 189 93 72 63
39 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 5042 5042
Size: 7816
SfoI GGC'GCC
Cuts at: 3936 3936
Size: 7816
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7816
SmaI CCC'GGG
Cuts at: 1872 3722 5063 5143 1872
Size: 1850 1341 80 4545
Fragments arranged by size:
4545 1850 1341 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2354 4208 5920 6821 7083
Size: 197 926 309 102 1854 1712 901 262
Cuts at: 7083 820
Size: 1553
Fragments arranged by size:
1854 1712 1553 926 901 309 262 197
102
SnaBI TAC'GTA
Cuts at: 493 5607 493
Size: 5114 2702
SpeI A'CTAG_T
Cuts at: 152 3415 152
Size: 3263 4553
SphI G_CATG'C
Cuts at: 1132 3329 3448 5174 1132
Size: 2197 119 1726 3774
Fragments arranged by size:
3774 2197 1726 119
SspI AAT'ATT
Cuts at: 5 52 5740 5
Size: 47 5688 2081
Fragments arranged by size:
5688 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7816
StyI C'CwwG_G
Cuts at: 513 1065 2052 2139 3124 4266 4366 4836 5013
Size: 552 987 87 985 1142 100 470 177
Cuts at: 5013 5094 5255 513
Size: 81 161 3074
Fragments arranged by size:
3074 1142 987 985 552 470 177 161
100 87 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 4394 4658 5003 5843 5860 6013
Size: 1255 2079 735 264 345 840 17 153
Cuts at: 6013 6198 6643 325
Size: 185 445 1498
Fragments arranged by size:
2079 1498 1255 840 735 445 345 264
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6062 7774 96
Size: 274 80 33 51 1443 4085 1712 138
Fragments arranged by size:
4085 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 4479 4511 4513 4545 7776 7808 3392
Size: 32 1055 32 2 32 3231 32 3400
Fragments arranged by size:
3400 3231 1055 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7816
XbaI T'CTAG_A
Cuts at: 5152 5152
Size: 7816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4549 4549
Size: 7816
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7816
XmaI C'CCGG_G
Cuts at: 1870 3720 5061 5141 1870
Size: 1850 1341 80 4545
Fragments arranged by size:
4545 1850 1341 80
XmnI GAAnn'nnTTC
Cuts at: 5945 5945
Size: 7816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5624 276
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FseI NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SexAI SrfI SwaI