Restriction map for
pFN21AA0598
(Circular) (Six-base) MAPSORT of: pFN21AA0598.seq Check: 1348 from: 1 to: 6388
pFN21AA0598 6388 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3321 3731 1937
Size: 1384 410 4594
Fragments arranged by size:
4594 1384 410
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 4451 5835 6103 1632
Size: 579 2240 1384 268 1917
Fragments arranged by size:
2240 1917 1384 579 268
AfeI AGC'GCT
Cuts at: 2675 2885 2675
Size: 210 6178
AflII C'TTAA_G
Cuts at: 820 1017 2531 3505 820
Size: 197 1514 974 3703
Fragments arranged by size:
3703 1514 974 197
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 3167 5117 3167
Size: 1950 4438
AleI CACnn'nnGTG
Cuts at: 1166 2869 1166
Size: 1703 4685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 3490 4131 5703 3490
Size: 641 1572 4175
Fragments arranged by size:
4175 1572 641
ApaI G_GGCC'C
Cuts at: 2084 2308 3504 3585 2084
Size: 224 1196 81 4887
Fragments arranged by size:
4887 1196 224 81
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2130 2573 3697 3923 4075 6184 1493
Size: 637 443 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 637 443 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2019 2465 4942 160
Size: 872 987 446 2477 1606
Fragments arranged by size:
2477 1606 987 872 446
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2617 3563 3713 1870
Size: 73 674 946 150 4545
Fragments arranged by size:
4545 946 674 150 73
AvrII C'CTAG_G
Cuts at: 3496 3496
Size: 6388
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 2308 2597 3372 3504 3542 3585 3707
Size: 693 224 289 775 132 38 43 122
Cuts at: 3707 1391
Size: 4072
Fragments arranged by size:
4072 775 693 289 224 132 122 43
38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2532 3472 3686 928
Size: 971 633 940 214 3630
Fragments arranged by size:
3630 971 940 633 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BfrBI ATG'CAT
Cuts at: 3449 3449
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 3355 3589
Size: 107 122 71 631 605 407 1276 234
Cuts at: 3589 4999 136
Size: 1410 1525
Fragments arranged by size:
1525 1410 1276 631 605 407 234 122
107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3398 3805 3398
Size: 407 5981
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2108 2308 3504 3585 4449 5605 1790
Size: 294 24 200 1196 81 864 1156 2573
Fragments arranged by size:
2573 1196 1156 864 294 200 81 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 4119 5077
Size: 416 53 83 399 24 798 1904 958
Cuts at: 5077 442
Size: 1753
Fragments arranged by size:
1904 1753 958 798 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3344 5048 1239
Size: 2105 1704 2579
Fragments arranged by size:
2579 2105 1704
Bpu10I CC'TnA_GC
Cuts at: 3176 3176
Size: 6388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4513 5378 5676 4513
Size: 865 298 5225
Fragments arranged by size:
5225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2913 5051 882
Size: 617 1414 2138 2219
Fragments arranged by size:
2219 2138 1414 617
BsaAI yAC'GTr
Cuts at: 493 1204 2112 2659 4179 493
Size: 711 908 547 1520 2702
Fragments arranged by size:
2702 1520 908 711 547
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 3559 4195 4577
Size: 53 83 186 936 1161 865 636 382
Cuts at: 4577 5212 275
Size: 635 1451
Fragments arranged by size:
1451 1161 936 865 636 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3570 4821 5219 5493 5640 6051 3570
Size: 1251 398 274 147 411 3907
Fragments arranged by size:
3907 1251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3182 3212 1178
Size: 30 165 30 1779 30 4354
Fragments arranged by size:
4354 1779 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 1186
Size: 1438 4950
BseYI C'CCAG_C
Cuts at: 2354 2363 5591 6087 2354
Size: 9 3228 496 2655
Fragments arranged by size:
3228 2655 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2813 1163
Size: 573 1077 4738
Fragments arranged by size:
4738 1077 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 4449 4534 5605 2664
Size: 1785 85 1071 3447
Fragments arranged by size:
3447 1785 1071 85
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2108 2308 2597 2664 3372 3504
Size: 399 294 24 200 289 67 708 132
Cuts at: 3504 3542 3585 3707 4449 4534 5605 1391
Size: 38 43 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 708 399 294 289 200
132 122 85 67 43 38 24
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3640 3743 844
Size: 1209 1587 103 3489
Fragments arranged by size:
3489 1587 1209 103
BsrBI CCG'CTC
Cuts at: 1394 1823 2682 4275 1394
Size: 429 859 1593 3507
Fragments arranged by size:
3507 1593 859 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5032 5219 1700
Size: 110 3222 187 2869
Fragments arranged by size:
3222 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 3310 1925
Size: 1385 5003
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2022 2545 3551 794
Size: 1228 523 1006 3631
Fragments arranged by size:
3631 1228 1006 523
BstBI TT'CG_AA
Cuts at: 3227 5192 3227
Size: 1965 4423
BstEII G'GTnAC_C
Cuts at: 1184 2093 1184
Size: 909 5479
BstXI CCAn_nnnn'nTGG
Cuts at: 2371 2871 2371
Size: 500 5888
BstYI r'GATC_y
Cuts at: 1972 2973 3718 4085 4470 4487 5939 6025 6037
Size: 1001 745 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 745 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2835 2835
Size: 6388
BtgI C'CryG_G
Cuts at: 513 1065 2931 3237 513
Size: 552 1866 306 3664
Fragments arranged by size:
3664 1866 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 4621
Fragments arranged by size:
4621 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2011 3269 3305 3904 4698 4718 2011
Size: 1258 36 599 794 20 3681
Fragments arranged by size:
3681 1258 794 599 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DraIII CAC_nnn'GTG
Cuts at: 3060 3060
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2961 3835 4724
Size: 54 1425 50 275 18 1131 874 889
Cuts at: 4724 6353 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1131 889 874 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 2839 2888 4975 5349 5495
Size: 1064 138 1149 101 49 2087 374 146
Cuts at: 5495 387
Size: 1280
Fragments arranged by size:
2087 1280 1149 1064 374 146 138 101
49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 3344 4451 5048 5835 6103 1239
Size: 393 579 1133 1107 597 787 268 1524
Fragments arranged by size:
1524 1133 1107 787 597 579 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 2080 2081 2304 3181 3500 3501 3581 3627 3652
Size: 1 223 877 319 1 80 46 25
Cuts at: 3652 2080
Size: 4816
Fragments arranged by size:
4816 877 319 223 80 46 25 1
1
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
FspI TGC'GCA
Cuts at: 2702 4894 2702
Size: 2192 4196
HaeII r_GCGC'y
Cuts at: 1678 2677 2887 5535 1678
Size: 999 210 2648 2531
Fragments arranged by size:
2648 2531 999 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 3153 3185 3434
Size: 32 135 32 660 32 889 32 249
Cuts at: 3434 3466 5029 5061 5103 5135 1373
Size: 32 1563 32 42 32 2626
Fragments arranged by size:
2626 1563 889 660 249 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3732 3978 669
Size: 988 281 130 1664 246 3079
Fragments arranged by size:
3079 1664 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3477 5502 5686 1305
Size: 2172 2025 184 2007
Fragments arranged by size:
2172 2025 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4566
Fragments arranged by size:
4566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2869 3350 4346 4705 4864 518
Size: 648 1703 481 996 359 159 2042
Fragments arranged by size:
2042 1703 996 648 481 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 3239 3277 3804 4481 5629 5874 1606
Size: 429 1204 38 527 677 1148 245 2120
Fragments arranged by size:
2120 1204 1148 677 527 429 245 38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5836
NdeI CA'TA_TG
Cuts at: 387 2550 387
Size: 2163 4225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NsiI A_TGCA'T
Cuts at: 3451 3451
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3295 3746 5291 1132
Size: 97 2066 451 1545 2229
Fragments arranged by size:
2229 2066 1545 451 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6388
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3060 1108
Size: 1952 4436
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 2112 2112
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 4457 4489 5970 6002 2828
Size: 32 1597 32 1481 32 3214
Fragments arranged by size:
3214 1597 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 3652 3181
Size: 471 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PspOMI G'GGCC_C
Cuts at: 2080 2304 3500 3581 2080
Size: 224 1196 81 4887
Fragments arranged by size:
4887 1196 224 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 6356
PstI C_TGCA'G
Cuts at: 830 2991 3276 3740 830
Size: 2161 285 464 3478
Fragments arranged by size:
3478 2161 464 285
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2035 3277 2035
Size: 1242 5146
SacII CC_GC'GG
Cuts at: 3240 3240
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SanDI GG'GwC_CC
Cuts at: 3181 3181
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2987 3272 3622 3736 4871 5552 5743
Size: 219 1942 285 350 114 1135 681 191
Cuts at: 5743 826
Size: 1471
Fragments arranged by size:
1942 1471 1135 681 350 285 219 191
114
SfiI GGCCn_nnn'nGGCC
Cuts at: 3589 3589
Size: 6388
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 3505 4492 5393 5655 820
Size: 197 926 588 974 987 901 262 1553
Fragments arranged by size:
1553 987 974 926 901 588 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3295 3746 1132
Size: 2163 451 3774
Fragments arranged by size:
3774 2163 451
SspI AAT'ATT
Cuts at: 5 52 2431 4312 5
Size: 47 2379 1881 2081
Fragments arranged by size:
2379 2081 1881 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 5197
StyI C'CwwG_G
Cuts at: 513 1065 2074 2274 3496 3827 513
Size: 552 1009 200 1222 331 3074
Fragments arranged by size:
3074 1222 1009 552 331 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4415 4432 4585 4770 5215 325
Size: 1255 2835 17 153 185 445 1498
Fragments arranged by size:
2835 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3198 3462 4634
Size: 274 80 33 51 1443 1221 264 1172
Cuts at: 4634 6346 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1221 1172 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2958 2990 6348 6380 2399
Size: 32 527 32 3358 32 2407
Fragments arranged by size:
3358 2407 527 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 2744 4517 2744
Size: 1773 4615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspXI RsrII SacI
SapI SexAI SfoI SrfI SwaI XcmI