Restriction map for
pFN21AA0597
(Circular) (Six-base) MAPSORT of: pFN21AA0597.seq Check: 8142 from: 1 to: 7435
pFN21AA0597 7435 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5245 278
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4778 1937
Size: 2841 4594
Acc65I G'GTAC_C
Cuts at: 4756 4756
Size: 7435
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 3584 3671 5498 6882 7150 1632
Size: 1200 752 87 1827 1384 268 1917
Fragments arranged by size:
1917 1827 1384 1200 752 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7238
AflIII A'CryG_T
Cuts at: 1203 2869 2974 6334 7311 1203
Size: 1666 105 3360 977 1327
Fragments arranged by size:
3360 1666 1327 977 105
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 3350 6164 3350
Size: 2814 4621
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2196 1184
Size: 1012 6423
AlwNI CAG_nnn'CTG
Cuts at: 3649 5178 6750 3649
Size: 1529 1572 4334
Fragments arranged by size:
4334 1572 1529
ApaLI G'TGCA_C
Cuts at: 2458 5492 6648 2458
Size: 3034 1156 3245
Fragments arranged by size:
3245 3034 1156
ApoI r'AATT_y
Cuts at: 1493 2416 3077 3370 3886 4744 4970 5122 7231
Size: 923 661 293 516 858 226 152 2109
Cuts at: 7231 1493
Size: 1697
Fragments arranged by size:
2109 1697 923 858 661 516 293 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5989 160
Size: 872 4957 1606
Fragments arranged by size:
4957 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 3584 4760 1870
Size: 73 1641 1176 4545
Fragments arranged by size:
4545 1641 1176 73
BamHI G'GATC_C
Cuts at: 4765 5132 4765
Size: 367 7068
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4756 6211 618
Size: 325 217 627 2969 1455 1842
Fragments arranged by size:
2969 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2378 2767 4029 4215 4754 1391
Size: 987 389 1262 186 539 4072
Fragments arranged by size:
4072 1262 987 539 389 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2866 3292 3910 4166 928
Size: 971 129 838 426 618 256 4197
Fragments arranged by size:
4197 971 838 618 426 256 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5626 5660 1903
Size: 34 3689 34 3678
Fragments arranged by size:
3689 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 5327 6537 3680
Size: 1647 1210 4578
Fragments arranged by size:
4578 1647 1210
BclI T'GATC_A
Cuts at: 1548 2620 4152 1548
Size: 1072 1532 4831
Fragments arranged by size:
4831 1532 1072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2218 6046 136
Size: 107 122 71 631 605 546 3828 1525
Fragments arranged by size:
3828 1525 631 605 546 122 107 71
BglII A'GATC_T
Cuts at: 3086 7430 3086
Size: 4344 3091
BlpI GC'TnA_GC
Cuts at: 4852 4852
Size: 7435
Bme1580I G_kGCm'C
Cuts at: 1790 2462 4297 5496 6652 1790
Size: 672 1835 1199 1156 2573
Fragments arranged by size:
2573 1835 1199 1156 672
BmgBI CAC'GTC
Cuts at: 1318 2457 2977 1318
Size: 1139 520 5776
Fragments arranged by size:
5776 1139 520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3110 4300 5166
Size: 416 53 83 399 24 1693 1190 866
Cuts at: 5166 6124 442
Size: 958 1753
Fragments arranged by size:
1753 1693 1190 958 866 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2568 3237 3549 3824 4209 6095 1239
Size: 948 381 669 312 275 385 1886 2579
Fragments arranged by size:
2579 1886 948 669 385 381 312 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5560 6425 6723 5560
Size: 865 298 6272
Fragments arranged by size:
6272 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3133 3714 4052 4149 6098 882
Size: 617 1634 581 338 97 1949 2219
Fragments arranged by size:
2219 1949 1634 617 581 338 97
BsaAI yAC'GTr
Cuts at: 493 1204 3503 5226 493
Size: 711 2299 1723 2702
Fragments arranged by size:
2702 2299 1723 711
BsaBI GATnn'nnATC
Cuts at: 3390 7429 3390
Size: 4039 3396
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5242 5624 6259 275
Size: 53 83 186 936 3709 382 635 1451
Fragments arranged by size:
3709 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 5868 6266 6540 6687 7098 4395
Size: 1473 398 274 147 411 4732
Fragments arranged by size:
4732 1473 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3296 3326 3840 3870 1178
Size: 30 165 30 1893 30 514 30 4743
Fragments arranged by size:
4743 1893 514 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 2714 2717 2729 4257 1186
Size: 1525 3 3 12 1528 4364
Fragments arranged by size:
4364 1528 1525 12 3 3
BseYI C'CCAG_C
Cuts at: 6638 7134 6638
Size: 496 6939
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 1163
Size: 573 886 5976
Fragments arranged by size:
5976 886 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2378 2462 2521 4029 5496 5581 6652 2378
Size: 84 59 1508 1467 85 1071 3161
Fragments arranged by size:
3161 1508 1467 1071 85 84 59
BsmI GAATG_Cn'
Cuts at: 2123 2471 2791 2838 4946 5039 2123
Size: 348 320 47 2108 93 4519
Fragments arranged by size:
4519 2108 348 320 93 47
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 2462 2521 2767 4029 4215 4297
Size: 399 588 84 59 246 1262 186 82
Cuts at: 4297 4754 5496 5581 6652 1391
Size: 457 742 85 1071 2174
Fragments arranged by size:
2174 1262 1071 742 588 457 399 246
186 85 84 82 59
BspEI T'CCGG_A
Cuts at: 4395 7098 4395
Size: 2703 4732
BspHI T'CATG_A
Cuts at: 4149 4155 5324 7054 4149
Size: 6 1169 1730 4530
Fragments arranged by size:
4530 1730 1169 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4790 844
Size: 3946 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5322 1394
Size: 429 3499 3507
Fragments arranged by size:
3507 3499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4320 5930 6104 65
Size: 1183 3072 1610 174 1396
Fragments arranged by size:
3072 1610 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6079 6266 1700
Size: 110 4269 187 2869
Fragments arranged by size:
4269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 3637 4667 5495 6507 3637
Size: 1030 828 1012 4565
Fragments arranged by size:
4565 1030 1012 828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7435
BstBI TT'CG_AA
Cuts at: 6239 6239
Size: 7435
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7435
BstYI r'GATC_y
Cuts at: 1972 3086 3697 4765 5132 5517 5534 6986 7072
Size: 1114 611 1068 367 385 17 1452 86
Cuts at: 7072 7084 7430 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1114 1068 611 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4104 4244 4104
Size: 140 7295
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2509 4280 513
Size: 552 939 9 496 1771 3668
Fragments arranged by size:
3668 1771 939 552 496 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6696
Fragments arranged by size:
6696 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2658 3650 4951 5745 5765 2265
Size: 393 992 1301 794 20 3935
Fragments arranged by size:
3935 1301 992 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7400
DraI TTT'AAA
Cuts at: 2892 3885 4739 2892
Size: 993 854 5588
Fragments arranged by size:
5588 993 854
DraIII CAC_nnn'GTG
Cuts at: 2466 2468 2466
Size: 2 7433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6442 809
Size: 507 5126 1802
Fragments arranged by size:
5126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3979 4882 5771
Size: 54 1425 50 275 18 2149 903 889
Cuts at: 5771 7400 8
Size: 1629 43
Fragments arranged by size:
2149 1629 1425 903 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2808 5365 1353
Size: 1335 120 2557 3423
Fragments arranged by size:
3423 2557 1335 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4595 6022 6396 6542 387
Size: 1064 138 2972 34 1427 374 146 1280
Fragments arranged by size:
2972 1427 1280 1064 374 146 138 34
EcoICRI GAG'CTC
Cuts at: 2376 4027 2376
Size: 1651 5784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2568 2832 3237 3549 3584 3671
Size: 393 555 381 264 405 312 35 87
Cuts at: 3671 3824 4209 5498 6095 6882 7150 1239
Size: 153 385 1289 597 787 268 1524
Fragments arranged by size:
1524 1289 787 597 555 405 393 385
381 312 268 264 153 87 35
EcoNI CCTnn'n_nnAGG
Cuts at: 4506 4506
Size: 7435
EcoO109I rG'GnC_Cy
Cuts at: 3851 4343 3851
Size: 492 6943
EcoRI G'AATT_C
Cuts at: 4744 5122 4744
Size: 378 7057
EcoRV GAT'ATC
Cuts at: 3190 3190
Size: 7435
FspI TGC'GCA
Cuts at: 5941 5941
Size: 7435
HaeII r_GCGC'y
Cuts at: 1678 4621 6582 1678
Size: 2943 1961 2531
Fragments arranged by size:
2943 2531 1961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 3037 3069 3336
Size: 32 135 32 818 32 615 32 267
Cuts at: 3336 3368 4330 4362 6076 6108 6150 6182 1373
Size: 32 962 32 1714 32 42 32 2626
Fragments arranged by size:
2626 1714 962 818 615 267 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2409 3760 4661 4779 5025 669
Size: 988 281 471 1351 901 118 246 3079
Fragments arranged by size:
3079 1351 988 901 471 281 246 118
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7435
HpaI GTT'AAC
Cuts at: 3760 5025 3760
Size: 1265 6170
KpnI G_GTAC'C
Cuts at: 4760 4760
Size: 7435
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 6549 6733 1305
Size: 1206 4038 184 2007
Fragments arranged by size:
4038 2007 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5613
Fragments arranged by size:
5613 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2574 4154 5393 5752 5911 518
Size: 648 913 495 1580 1239 359 159 2042
Fragments arranged by size:
2042 1580 1239 913 648 495 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2642 4037 4851 5528 6676 6921 1606
Size: 409 627 1395 814 677 1148 245 2120
Fragments arranged by size:
2120 1395 1148 814 677 627 409 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2509 4280 513
Size: 552 939 505 1771 3668
Fragments arranged by size:
3668 1771 939 552 505
NdeI CA'TA_TG
Cuts at: 387 2027 387
Size: 1640 5795
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7435
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NspI r_CATG'y
Cuts at: 1132 1229 2340 2540 2873 3776 4313 4793 6338
Size: 97 1111 200 333 903 537 480 1545
Cuts at: 6338 1132
Size: 2229
Fragments arranged by size:
2229 1545 1111 903 537 480 333 200
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7435
PciI A'CATG_T
Cuts at: 2869 6334 2869
Size: 3465 3970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7435
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3567 3599 5504 5536 7017 7049 3567
Size: 32 1905 32 1481 32 3953
Fragments arranged by size:
3953 1905 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4343 4343
Size: 7435
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7435
PsiI TTA'TAA
Cuts at: 5005 5005
Size: 7435
PspXI vC'TCGA_Gb
Cuts at: 3584 3584
Size: 7435
PstI C_TGCA'G
Cuts at: 830 4787 830
Size: 3957 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
PvuII CAG'CTG
Cuts at: 2642 4037 2642
Size: 1395 6040
SacI G_AGCT'C
Cuts at: 2378 4029 2378
Size: 1651 5784
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 7435
SalI G'TCGA_C
Cuts at: 1936 4777 1936
Size: 2841 4594
SapI GCTCTTCn'nnn_
Cuts at: 2808 2808
Size: 7435
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 5683 7395 5683
Size: 1712 5723
SexAI A'CCwGG_T
Cuts at: 3346 3346
Size: 7435
SfcI C'TryA_G
Cuts at: 826 1045 3553 4783 5918 6599 6790 826
Size: 219 2508 1230 1135 681 191 1471
Fragments arranged by size:
2508 1471 1230 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 4762 1872
Size: 2890 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3584 5539 6440 6702 820
Size: 197 926 1641 1955 901 262 1553
Fragments arranged by size:
1955 1641 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3503 5226 493
Size: 3010 1723 2702
Fragments arranged by size:
3010 2702 1723
SpeI A'CTAG_T
Cuts at: 152 2854 152
Size: 2702 4733
SphI G_CATG'C
Cuts at: 1132 2340 2540 3776 4313 4793 1132
Size: 1208 200 1236 537 480 3774
Fragments arranged by size:
3774 1236 1208 537 480 200
SspI AAT'ATT
Cuts at: 5 52 5359 5
Size: 47 5307 2081
Fragments arranged by size:
5307 2081 47
StuI AGG'CCT
Cuts at: 1517 3000 3813 1517
Size: 1483 813 5139
Fragments arranged by size:
5139 1483 813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2509 3198 4254 4280 4874 513
Size: 552 939 505 689 1056 26 594 3074
Fragments arranged by size:
3074 1056 939 689 594 552 505 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2031 2817 2869 5462 5479 5632 5817
Size: 1255 451 786 52 2593 17 153 185
Cuts at: 5817 6262 325
Size: 445 1498
Fragments arranged by size:
2593 1498 1255 786 451 445 185 153
52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2087 3397 5681
Size: 274 80 33 51 1443 110 1310 2284
Cuts at: 5681 7393 96
Size: 1712 138
Fragments arranged by size:
2284 1712 1443 1310 274 138 110 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4101 4133 4287 4319 7395 7427 4101
Size: 32 154 32 3076 32 4109
Fragments arranged by size:
4109 3076 154 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7435
XbaI T'CTAG_A
Cuts at: 4771 4771
Size: 7435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4287 4287
Size: 7435
XhoI C'TCGA_G
Cuts at: 1943 3584 1943
Size: 1641 5794
XmaI C'CCGG_G
Cuts at: 1870 4760 1870
Size: 2890 4545
XmnI GAAnn'nnTTC
Cuts at: 3813 5564 3813
Size: 1751 5684
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5243 276
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PsrI RsrII SanDI
SfiI SfoI SrfI SwaI