Restriction map for
pFN21AA0595
(Circular) (Six-base) MAPSORT of: pFN21AA0595.seq Check: 299 from: 1 to: 9697
pFN21AA0595 9697 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 3349 4531 4643 2146
Size: 1203 1182 112 7200
Fragments arranged by size:
7200 1203 1182 112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7507 278
Size: 53 83 186 936 5971 2468
Fragments arranged by size:
5971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 3495 3735 4988 7040 1937
Size: 1527 31 240 1253 2052 4594
Fragments arranged by size:
4594 2052 1527 1253 240 31
Acc65I G'GTAC_C
Cuts at: 4217 7018 4217
Size: 2801 6896
AclI AA'CG_TT
Cuts at: 1563 7824 8197 1563
Size: 6261 373 3063
Fragments arranged by size:
6261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2752 2978 4197 5286 5487 5813 5820 7760
Size: 1120 226 1219 1089 201 326 7 1940
Cuts at: 7760 9144 9412 1632
Size: 1384 268 1917
Fragments arranged by size:
1940 1917 1384 1219 1120 1089 326 268
226 201 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9500
AflIII A'CryG_T
Cuts at: 1203 4931 8596 9573 1203
Size: 3728 3665 977 1327
Fragments arranged by size:
3728 3665 1327 977
AgeI A'CCGG_T
Cuts at: 5649 8528 5649
Size: 2879 6818
AhdI GACnn_n'nnGTC
Cuts at: 8426 8426
Size: 9697
AleI CACnn'nnGTG
Cuts at: 1166 6732 1166
Size: 5566 4131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9697
AlwNI CAG_nnn'CTG
Cuts at: 2161 2423 2894 3203 5186 6926 7440 9012 2161
Size: 262 471 309 1983 1740 514 1572 2846
Fragments arranged by size:
2846 1983 1740 1572 514 471 309 262
ApaI G_GGCC'C
Cuts at: 2060 2216 2568 4947 4961 6020 2060
Size: 156 352 2379 14 1059 5737
Fragments arranged by size:
5737 2379 1059 352 156 14
ApaLI G'TGCA_C
Cuts at: 6718 7754 8910 6718
Size: 1036 1156 7505
Fragments arranged by size:
7505 1156 1036
ApoI r'AATT_y
Cuts at: 1493 3604 6938 7006 7232 7384 9493 1493
Size: 2111 3334 68 226 152 2109 1697
Fragments arranged by size:
3334 2111 2109 1697 226 152 68
AscI GG'CGCG_CC
Cuts at: 2012 2012
Size: 9697
AseI AT'TA_AT
Cuts at: 160 1032 8251 160
Size: 872 7219 1606
Fragments arranged by size:
7219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9697
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2478 3567 3579 6054 7022 1870
Size: 73 104 431 1089 12 2475 968 4545
Fragments arranged by size:
4545 2475 1089 968 431 104 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3465 3498 3465
Size: 33 9664
BamHI G'GATC_C
Cuts at: 7027 7394 7027
Size: 367 9330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4217 4543 4831 4856
Size: 325 217 627 1529 901 326 288 25
Cuts at: 4856 4894 7018 8473 618
Size: 38 2124 1455 1842
Fragments arranged by size:
2124 1842 1529 1455 901 627 326 325
288 217 38 25
BanII G_rGCy'C
Cuts at: 1391 2060 2086 2216 2489 2568 3373 3580 4947
Size: 669 26 130 273 79 805 207 1367
Cuts at: 4947 4961 5146 5688 5882 5960 6020 7016 1391
Size: 14 185 542 194 78 60 996 4072
Fragments arranged by size:
4072 1367 996 805 669 542 273 207
194 185 130 79 78 60 26 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4421 4700 6339 6468 6631 6915 928
Size: 971 2522 279 1639 129 163 284 3710
Fragments arranged by size:
3710 2522 1639 971 284 279 163 129
BbvCI CC'TCA_GC
Cuts at: 3440 6100 6190 6777 3440
Size: 2660 90 587 6360
Fragments arranged by size:
6360 2660 587 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6210 6244 7888 7922 1903
Size: 34 4273 34 1644 34 3678
Fragments arranged by size:
4273 3678 1644 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2825 7589 8799 2825
Size: 4764 1210 3723
Fragments arranged by size:
4764 3723 1210
BclI T'GATC_A
Cuts at: 1548 3897 6067 1548
Size: 2349 2170 5178
Fragments arranged by size:
5178 2349 2170
BfrBI ATG'CAT
Cuts at: 2833 6042 2833
Size: 3209 6488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4771 5678
Size: 107 122 71 631 605 337 2762 907
Cuts at: 5678 6273 8308 136
Size: 595 2035 1525
Fragments arranged by size:
2762 2035 1525 907 631 605 595 337
122 107 71
BglII A'GATC_T
Cuts at: 2403 9692 2403
Size: 7289 2408
BlpI GC'TnA_GC
Cuts at: 3885 7114 3885
Size: 3229 6468
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2216 2568 3213 3321 4614 4836 4897
Size: 270 156 352 645 108 1293 222 61
Cuts at: 4897 4947 4961 5066 6020 6722 7758 8914 1790
Size: 50 14 105 954 702 1036 1156 2573
Fragments arranged by size:
2573 1293 1156 1036 954 702 645 352
270 222 156 108 105 61 50 14
BmgBI CAC'GTC
Cuts at: 1318 4088 1318
Size: 2770 6927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 2562 2570
Size: 416 53 83 399 24 1126 19 8
Cuts at: 2570 3083 3899 4046 4341 4668 4904 7428 8386
Size: 513 816 147 295 327 236 2524 958
Cuts at: 8386 442
Size: 1753
Fragments arranged by size:
2524 1753 1126 958 816 513 416 399
327 295 236 147 83 53 24 19
8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9697
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2926 5974 2506
Size: 420 3048 6229
Fragments arranged by size:
6229 3048 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2698 3062 3101 3206 3533 4026 4998
Size: 1142 317 364 39 105 327 493 972
Cuts at: 4998 5052 5552 5679 8357 1239
Size: 54 500 127 2678 2579
Fragments arranged by size:
2678 2579 1142 972 500 493 364 327
317 127 105 54 39
Bpu10I CC'TnA_GC
Cuts at: 3440 4497 6100 6190 6777 3440
Size: 1057 1603 90 587 6360
Fragments arranged by size:
6360 1603 1057 587 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3002 4469 6232 7822 8687 8985 3002
Size: 1467 1763 1590 865 298 3714
Fragments arranged by size:
3714 1763 1590 1467 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2645 2674 3112 4567 5306 5315 8360
Size: 617 1146 29 438 1455 739 9 3045
Cuts at: 8360 882
Size: 2219
Fragments arranged by size:
3045 2219 1455 1146 739 617 438 29
9
BsaAI yAC'GTr
Cuts at: 493 1204 5453 7488 493
Size: 711 4249 2035 2702
Fragments arranged by size:
4249 2702 2035 711
BsaBI GATnn'nnATC
Cuts at: 6520 9691 6520
Size: 3171 6526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7504 7886 8521 275
Size: 53 83 186 936 5971 382 635 1451
Fragments arranged by size:
5971 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2065 5649 5904 6309 8130 8528 8802 8949 9360
Size: 3584 255 405 1821 398 274 147 411
Cuts at: 9360 2065
Size: 2402
Fragments arranged by size:
3584 2402 1821 411 405 398 274 255
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4113 4130 4143 4160 6160
Size: 30 165 30 2710 17 13 17 2000
Cuts at: 6160 6190 1178
Size: 30 4685
Fragments arranged by size:
4685 2710 2000 165 30 30 30 17
17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2261 2479 2765 2768 2864 2902 3275
Size: 1000 75 218 286 3 96 38 373
Cuts at: 3275 4121 5100 5136 5297 5562 6728 6794 6892
Size: 846 979 36 161 265 1166 66 98
Cuts at: 6892 1186
Size: 3991
Fragments arranged by size:
3991 1166 1000 979 846 373 286 265
218 161 98 96 75 66 38 36
3
BseYI C'CCAG_C
Cuts at: 2209 2275 2703 2724 3991 4125 4405 4475 5413
Size: 66 428 21 1267 134 280 70 938
Cuts at: 5413 6019 6919 8900 9396 2209
Size: 606 900 1981 496 2510
Fragments arranged by size:
2510 1981 1267 938 900 606 496 428
280 134 70 66 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 4113 4610 1163
Size: 573 744 1633 497 6250
Fragments arranged by size:
6250 1633 744 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7147 7908 8936 1540
Size: 275 186 5146 761 1028 2301
Fragments arranged by size:
5146 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3132 3373 5146 5387 6722 7758 7843 8914 3132
Size: 241 1773 241 1335 1036 85 1071 3915
Fragments arranged by size:
3915 1773 1335 1071 1036 241 241 85
BsmI GAATG_Cn'
Cuts at: 2448 7208 7301 2448
Size: 4760 93 4844
Fragments arranged by size:
4844 4760 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2022 2022
Size: 9697
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2086 2216 2489 2568 3132 3213
Size: 399 270 26 130 273 79 564 81
Cuts at: 3213 3321 3373 3580 4614 4836 4897 4947 4961
Size: 108 52 207 1034 222 61 50 14
Cuts at: 4961 5066 5146 5387 5688 5882 5960 6020 6722
Size: 105 80 241 301 194 78 60 702
Cuts at: 6722 7016 7758 7843 8914 1391
Size: 294 742 85 1071 2174
Fragments arranged by size:
2174 1071 1034 742 702 564 399 301
294 273 270 241 222 207 194 130
108 105 85 81 80 79 78 61
60 52 50 26 14
BspEI T'CCGG_A
Cuts at: 2065 5904 9360 2065
Size: 3839 3456 2402
Fragments arranged by size:
3839 3456 2402
BspHI T'CATG_A
Cuts at: 7586 9316 7586
Size: 1730 7967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2146 2340 3349 4531 4643 6222 7052 844
Size: 1302 194 1009 1182 112 1579 830 3489
Fragments arranged by size:
3489 1579 1302 1182 1009 830 194 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 6510 7584 1394
Size: 429 322 4365 1074 3507
Fragments arranged by size:
4365 3507 1074 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 8192 8366 65
Size: 1183 6944 174 1396
Fragments arranged by size:
6944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3310 4272 5649 8341 8528 1700
Size: 110 1500 962 1377 2692 187 2869
Fragments arranged by size:
2869 2692 1500 1377 962 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9697
BssHII G'CGCG_C
Cuts at: 1925 2012 1925
Size: 87 9610
BssSI C'ACGA_G
Cuts at: 4133 7757 8769 4133
Size: 3624 1012 5061
Fragments arranged by size:
5061 3624 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2503 6598 794
Size: 1367 342 4095 3893
Fragments arranged by size:
4095 3893 1367 342
BstBI TT'CG_AA
Cuts at: 8501 8501
Size: 9697
BstEII G'GTnAC_C
Cuts at: 1184 3217 1184
Size: 2033 7664
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 4369 5530 2836
Size: 1533 1161 7003
Fragments arranged by size:
7003 1533 1161
BstYI r'GATC_y
Cuts at: 1972 2403 2614 3084 6094 7027 7394 7779 7796
Size: 431 211 470 3010 933 367 385 17
Cuts at: 7796 9248 9334 9346 9692 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3010 1977 1452 933 470 431 385 367
346 211 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2847 3024 3516 6138 6989 2847
Size: 177 492 2622 851 5555
Fragments arranged by size:
5555 2622 851 492 177
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 5137 5854 513
Size: 552 939 82 3051 717 4356
Fragments arranged by size:
4356 3051 939 717 552 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3087 487
Size: 683 56 1861 7097
Fragments arranged by size:
7097 1861 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 2270 2740 2889 3017 3786 3945 4101 4888
Size: 172 470 149 128 769 159 156 787
Cuts at: 4888 5741 6088 7213 8007 8027 2098
Size: 853 347 1125 794 20 3768
Fragments arranged by size:
3768 1125 853 794 787 769 470 347
172 159 156 149 128 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3775 3810 4674 4709 4989 5024 529
Size: 35 3211 35 864 35 280 35 5202
Fragments arranged by size:
5202 3211 864 280 35 35 35 35
DraI TTT'AAA
Cuts at: 7001 7001
Size: 9697
DraIII CAC_nnn'GTG
Cuts at: 5212 5212
Size: 9697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3645 5900 8704 809
Size: 507 2329 2255 2804 1802
Fragments arranged by size:
2804 2329 2255 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 2773 5134
Size: 54 1425 50 275 18 930 13 2361
Cuts at: 5134 5670 5748 6658 6805 7144 8033 9662 8
Size: 536 78 910 147 339 889 1629 43
Fragments arranged by size:
2361 1629 1425 930 910 889 536 339
275 147 78 54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 7144 1537
Size: 275 5332 4090
Fragments arranged by size:
5332 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2613 2662 2742 3386 4302 5630 6448 6454
Size: 1260 49 80 644 916 1328 818 6
Cuts at: 6454 6475 7627 1353
Size: 21 1152 3423
Fragments arranged by size:
3423 1328 1260 1152 916 818 644 80
49 21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6021 8284 8658 8804 387
Size: 1064 138 4432 2263 374 146 1280
Fragments arranged by size:
4432 2263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3371 5144 3371
Size: 1773 7924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2698 2752 2978 3062 3101 3206
Size: 393 749 317 54 226 84 39 105
Cuts at: 3206 3533 4026 4197 4998 5052 5286 5487 5552
Size: 327 493 171 801 54 234 201 65
Cuts at: 5552 5679 5813 5820 7760 8357 9144 9412 1239
Size: 127 134 7 1940 597 787 268 1524
Fragments arranged by size:
1940 1524 801 787 749 597 493 393
327 317 268 234 226 201 171 134
127 105 84 65 54 54 39 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2321
Size: 9697
EcoO109I rG'GnC_Cy
Cuts at: 2056 2057 2233 2564 3662 4472 4944 4957 4964
Size: 1 176 331 1098 810 472 13 7
Cuts at: 4964 5640 5913 6372 2056
Size: 676 273 459 5381
Fragments arranged by size:
5381 1098 810 676 472 459 331 273
176 13 7 1
EcoRI G'AATT_C
Cuts at: 3604 7006 7384 3604
Size: 3402 378 5917
Fragments arranged by size:
5917 3402 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5362 5452 6459 5362
Size: 90 1007 8600
Fragments arranged by size:
8600 1007 90
FspI TGC'GCA
Cuts at: 8203 8203
Size: 9697
HaeII r_GCGC'y
Cuts at: 1678 2120 2139 5980 8844 1678
Size: 442 19 3841 2864 2531
Fragments arranged by size:
3841 2864 2531 442 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 3230 3262 4130
Size: 32 135 32 447 32 1179 32 868
Cuts at: 4130 4162 5095 5127 5230 5262 5276 5308 5955
Size: 32 933 32 103 32 14 32 647
Cuts at: 5955 5987 6881 6913 8338 8370 8412 8444 1373
Size: 32 894 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 1179 933 894 868 647 447
135 103 42 32 32 32 32 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 3736 3766 3826 3841 3931
Size: 988 281 616 1182 30 60 15 90
Cuts at: 3931 4018 4048 5620 7041 7287 669
Size: 87 30 1572 1421 246 3079
Fragments arranged by size:
3079 1572 1421 1182 988 616 281 246
90 87 60 30 30 15
HindIII A'AGCT_T
Cuts at: 748 2666 748
Size: 1918 7779
HpaI GTT'AAC
Cuts at: 7287 7287
Size: 9697
KpnI G_GTAC'C
Cuts at: 4221 7022 4221
Size: 2801 6896
MfeI C'AATT_G
Cuts at: 7296 7296
Size: 9697
MluI A'CGCG_T
Cuts at: 9573 9573
Size: 9697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6330 6924 8811 8995 1305
Size: 5025 594 1887 184 2007
Fragments arranged by size:
5025 2007 1887 594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 2775 5136 5672 5750
Size: 54 1425 343 930 13 2361 536 78
Cuts at: 5750 6807 10
Size: 1057 2900
Fragments arranged by size:
2900 2361 1425 1057 930 536 343 78
54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 6732 7655 8014 8173 518
Size: 648 5566 923 359 159 2042
Fragments arranged by size:
5566 2042 923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2894 3068 3169 3593 5072 5828 6023
Size: 482 806 174 101 424 1479 756 195
Cuts at: 6023 6202 6411 7113 7790 8938 9183 1606
Size: 179 209 702 677 1148 245 2120
Fragments arranged by size:
2120 1479 1148 806 756 702 677 482
424 245 209 195 179 174 101
NcoI C'CATG_G
Cuts at: 513 1065 2004 5137 5854 513
Size: 552 939 3133 717 4356
Fragments arranged by size:
4356 3133 939 717 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9697
NotI GC'GGCC_GC
Cuts at: 7144 7144
Size: 9697
NsiI A_TGCA'T
Cuts at: 2835 6044 2835
Size: 3209 6488
NspI r_CATG'y
Cuts at: 1132 1229 4669 4935 4976 5256 7055 8600 1132
Size: 97 3440 266 41 280 1799 1545 2229
Fragments arranged by size:
3440 2229 1799 1545 280 266 97 41
PasI CC'CwG_GG
Cuts at: 1348 3294 3830 4108 4961 5331 1348
Size: 1946 536 278 853 370 5714
Fragments arranged by size:
5714 1946 853 536 370 278
PciI A'CATG_T
Cuts at: 4931 8596 4931
Size: 3665 6032
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2894 1108
Size: 1786 7911
PfoI T'CCnGG_A
Cuts at: 2817 3112 3132 2817
Size: 295 20 9382
Fragments arranged by size:
9382 295 20
PmeI GTTT'AAAC
Cuts at: 7001 7001
Size: 9697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2555 7766 7798 9279 9311 2523
Size: 32 5211 32 1481 32 2909
Fragments arranged by size:
5211 2909 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 4472 5913 6372 2233
Size: 2239 1441 459 5558
Fragments arranged by size:
5558 2239 1441 459
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9697
PsiI TTA'TAA
Cuts at: 7267 7267
Size: 9697
PspOMI G'GGCC_C
Cuts at: 2056 2212 2564 4943 4957 6016 2056
Size: 156 352 2379 14 1059 5737
Fragments arranged by size:
5737 2379 1059 352 156 14
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5446 5478 5446
Size: 32 9665
PstI C_TGCA'G
Cuts at: 830 2262 3793 3940 4428 4654 5833 6233 7049
Size: 1432 1531 147 488 226 1179 400 816
Cuts at: 7049 830
Size: 3478
Fragments arranged by size:
3478 1531 1432 1179 816 488 400 226
147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9697
PvuII CAG'CTG
Cuts at: 2894 3068 3169 3593 5072 5828 6023 6411 2894
Size: 174 101 424 1479 756 195 388 6180
Fragments arranged by size:
6180 1479 756 424 388 195 174 101
RsrII CG'GwC_CG
Cuts at: 2063 2063
Size: 9697
SacI G_AGCT'C
Cuts at: 3373 5146 3373
Size: 1773 7924
SacII CC_GC'GG
Cuts at: 2089 2089
Size: 9697
SalI G'TCGA_C
Cuts at: 1936 3734 7039 1936
Size: 1798 3305 4594
Fragments arranged by size:
4594 3305 1798
SapI GCTCTTCn'nnn_
Cuts at: 3386 6448 3386
Size: 3062 6635
SbfI CC_TGCA'GG
Cuts at: 4654 6233 7049 4654
Size: 1579 816 7302
Fragments arranged by size:
7302 1579 816
ScaI AGT'ACT
Cuts at: 4731 7945 9657 4731
Size: 3214 1712 4771
Fragments arranged by size:
4771 3214 1712
SexAI A'CCwGG_T
Cuts at: 3355 3355
Size: 9697
SfcI C'TryA_G
Cuts at: 826 1045 2258 3468 3789 3936 4424 4467 4650
Size: 219 1213 1210 321 147 488 43 183
Cuts at: 4650 5598 5829 6229 6412 7045 8180 8861 9052
Size: 948 231 400 183 633 1135 681 191
Cuts at: 9052 826
Size: 1471
Fragments arranged by size:
1471 1213 1210 1135 948 681 633 488
400 321 231 219 191 183 183 147
43
SfiI GGCCn_nnn'nGGCC
Cuts at: 6273 6273
Size: 9697
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9697
SmaI CCC'GGG
Cuts at: 1872 2480 3569 6056 7024 1872
Size: 608 1089 2487 968 4545
Fragments arranged by size:
4545 2487 1089 968 608
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 4448 6247 7801 8702 8964
Size: 197 926 1038 1467 1799 1554 901 262
Cuts at: 8964 820
Size: 1553
Fragments arranged by size:
1799 1554 1553 1467 1038 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 7488 493
Size: 6995 2702
SpeI A'CTAG_T
Cuts at: 152 3152 152
Size: 3000 6697
SphI G_CATG'C
Cuts at: 1132 4669 7055 1132
Size: 3537 2386 3774
Fragments arranged by size:
3774 3537 2386
SspI AAT'ATT
Cuts at: 5 52 7621 5
Size: 47 7569 2081
Fragments arranged by size:
7569 2081 47
StuI AGG'CCT
Cuts at: 1517 6143 1517
Size: 4626 5071
StyI C'CwwG_G
Cuts at: 513 1065 2004 2338 3213 3321 3597 4149 4889
Size: 552 939 334 875 108 276 552 740
Cuts at: 4889 4947 5137 5163 5358 5375 5854 6012 6739
Size: 58 190 26 195 17 479 158 727
Cuts at: 6739 7136 513
Size: 397 3074
Fragments arranged by size:
3074 939 875 740 727 552 552 479
397 334 276 195 190 158 108 58
26 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2958 2981 5427 6354 7724 7741 7894
Size: 1255 1378 23 2446 927 1370 17 153
Cuts at: 7894 8079 8524 325
Size: 185 445 1498
Fragments arranged by size:
2446 1498 1378 1370 1255 927 445 185
153 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2570 4729 5089
Size: 274 80 33 51 1443 593 2159 360
Cuts at: 5089 7943 9655 96
Size: 2854 1712 138
Fragments arranged by size:
2854 2159 1712 1443 593 360 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4113 4145 6067 6099 6160 6192 9657 9689 4113
Size: 32 1922 32 61 32 3465 32 4121
Fragments arranged by size:
4121 3465 1922 61 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 1316
Size: 717 8980
XbaI T'CTAG_A
Cuts at: 3667 7033 3667
Size: 3366 6331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2619 3301 3862 4335 5879 6393 2120
Size: 499 682 561 473 1544 514 5424
Fragments arranged by size:
5424 1544 682 561 514 499 473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9697
XmaI C'CCGG_G
Cuts at: 1870 2478 3567 6054 7022 1870
Size: 608 1089 2487 968 4545
Fragments arranged by size:
4545 2487 1089 968 608
XmnI GAAnn'nnTTC
Cuts at: 3525 7826 3525
Size: 4301 5396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7505 276
Size: 53 83 186 936 5971 2468
Fragments arranged by size:
5971 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BbeI BsiWI BstZ17I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI SanDI SfoI SrfI SwaI