Restriction map for
pFN21AA0594
(Circular) (Six-base) MAPSORT of: pFN21AA0594.seq Check: 7889 from: 1 to: 8008
pFN21AA0594 8008 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2030 5818 278
Size: 53 83 186 936 494 3788 2468
Fragments arranged by size:
3788 2468 936 494 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5217 5351 1937
Size: 3280 134 4594
Fragments arranged by size:
4594 3280 134
Acc65I G'GTAC_C
Cuts at: 5329 5329
Size: 8008
AclI AA'CG_TT
Cuts at: 1563 6135 6508 1563
Size: 4572 373 3063
Fragments arranged by size:
4572 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3053 3176 4644 5009 6071 7455 7723 1632
Size: 1421 123 1468 365 1062 1384 268 1917
Fragments arranged by size:
1917 1468 1421 1384 1062 365 268 123
AflII C'TTAA_G
Cuts at: 820 1017 2656 3529 820
Size: 197 1639 873 5299
Fragments arranged by size:
5299 1639 873 197
AflIII A'CryG_T
Cuts at: 1203 2924 3390 6907 7884 1203
Size: 1721 466 3517 977 1327
Fragments arranged by size:
3517 1721 1327 977 466
AgeI A'CCGG_T
Cuts at: 6839 6839
Size: 8008
AhdI GACnn_n'nnGTC
Cuts at: 2077 6737 2077
Size: 4660 3348
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8008
AlwNI CAG_nnn'CTG
Cuts at: 5751 7323 5751
Size: 1572 6436
ApaLI G'TGCA_C
Cuts at: 6065 7221 6065
Size: 1156 6852
ApoI r'AATT_y
Cuts at: 1493 2104 2561 3431 3987 4829 4914 4931 5254
Size: 611 457 870 556 842 85 17 323
Cuts at: 5254 5317 5543 5695 7804 1493
Size: 63 226 152 2109 1697
Fragments arranged by size:
2109 1697 870 842 611 556 457 323
226 152 85 63 17
AseI AT'TA_AT
Cuts at: 160 1032 4821 6562 160
Size: 872 3789 1741 1606
Fragments arranged by size:
3789 1741 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2089 2678 5333 1870
Size: 73 122 24 589 2655 4545
Fragments arranged by size:
4545 2655 589 122 73 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4515 4548 4515
Size: 33 7975
BamHI G'GATC_C
Cuts at: 5338 5705 5338
Size: 367 7641
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5280 5329 6784 618
Size: 325 217 627 3493 49 1455 1842
Fragments arranged by size:
3493 1842 1455 627 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2062 3456 5327 1391
Size: 671 1394 1871 4072
Fragments arranged by size:
4072 1871 1394 671
BbeI G_GCGC'C
Cuts at: 5284 5284
Size: 8008
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2030 3967 928
Size: 971 131 1937 4969
Fragments arranged by size:
4969 1937 971 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6199 6233 1903
Size: 34 4262 34 3678
Fragments arranged by size:
4262 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4168 5900 7110 4168
Size: 1732 1210 5066
Fragments arranged by size:
5066 1732 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8008
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6619 136
Size: 107 122 71 631 605 4947 1525
Fragments arranged by size:
4947 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3659 8003 3659
Size: 4344 3664
BlpI GC'TnA_GC
Cuts at: 5425 5425
Size: 8008
Bme1580I G_kGCm'C
Cuts at: 1790 6069 7225 1790
Size: 4279 1156 2573
Fragments arranged by size:
4279 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8008
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5739 6697 442
Size: 416 53 83 399 24 4322 958 1753
Fragments arranged by size:
4322 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8008
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3309 3309
Size: 8008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4073 6668 1239
Size: 2834 2595 2579
Fragments arranged by size:
2834 2595 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4118 4497 6133 6998 7296 4118
Size: 379 1636 865 298 4830
Fragments arranged by size:
4830 1636 865 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2064 2675 6671 882
Size: 617 565 611 3996 2219
Fragments arranged by size:
3996 2219 617 611 565
BsaAI yAC'GTr
Cuts at: 493 1204 3393 4034 5799 493
Size: 711 2189 641 1765 2702
Fragments arranged by size:
2702 2189 1765 711 641
BsaBI GATnn'nnATC
Cuts at: 8002 8002
Size: 8008
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 5281 5815 6197
Size: 53 83 186 936 494 3254 534 382
Cuts at: 6197 6832 275
Size: 635 1451
Fragments arranged by size:
3254 1451 936 635 534 494 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6441 6839 7113 7260 7671 6441
Size: 398 274 147 411 6778
Fragments arranged by size:
6778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2017 2047 3109 3139 5154
Size: 30 165 30 614 30 1062 30 2015
Cuts at: 5154 5184 1178
Size: 30 4002
Fragments arranged by size:
4002 2015 1062 614 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 3130 4549 4956 1186
Size: 1393 551 1419 407 4238
Fragments arranged by size:
4238 1419 1393 551 407
BseYI C'CCAG_C
Cuts at: 2043 7211 7707 2043
Size: 5168 496 2344
Fragments arranged by size:
5168 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4515 1163
Size: 573 2779 4656
Fragments arranged by size:
4656 2779 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5458 6219 7247 1540
Size: 275 186 3457 761 1028 2301
Fragments arranged by size:
3457 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 4059 6069 6154 7225 2062
Size: 1997 2010 85 1071 2845
Fragments arranged by size:
2845 2010 1997 1071 85
BsmI GAATG_Cn'
Cuts at: 5519 5612 5519
Size: 93 7915
BsmBI CGTCTCn'nnnn_
Cuts at: 3220 3220
Size: 8008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 3456 4059 5327 6069 6154 7225
Size: 399 272 1394 603 1268 742 85 1071
Cuts at: 7225 1391
Size: 2174
Fragments arranged by size:
2174 1394 1268 1071 742 603 399 272
85
BspEI T'CCGG_A
Cuts at: 7671 7671
Size: 8008
BspHI T'CATG_A
Cuts at: 3763 5897 7627 3763
Size: 2134 1730 4144
Fragments arranged by size:
4144 2134 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 5363 844
Size: 1470 3049 3489
Fragments arranged by size:
3489 3049 1470
BsrBI CCG'CTC
Cuts at: 1394 1823 5895 1394
Size: 429 4072 3507
Fragments arranged by size:
4072 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6503 6677 65
Size: 1183 5255 174 1396
Fragments arranged by size:
5255 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6652 6839 1700
Size: 110 4842 187 2869
Fragments arranged by size:
4842 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8008
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8008
BssSI C'ACGA_G
Cuts at: 6068 7080 6068
Size: 1012 6996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8008
BstBI TT'CG_AA
Cuts at: 4918 4936 6812 4918
Size: 18 1876 6114
Fragments arranged by size:
6114 1876 18
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8008
BstXI CCAn_nnnn'nTGG
Cuts at: 5250 5250
Size: 8008
BstYI r'GATC_y
Cuts at: 1972 3659 5338 5705 6090 6107 7559 7645 7657
Size: 1687 1679 367 385 17 1452 86 12
Cuts at: 7657 8003 1972
Size: 346 1977
Fragments arranged by size:
1977 1687 1679 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2544 2544
Size: 8008
BtgI C'CryG_G
Cuts at: 513 1065 2004 2816 3171 4569 513
Size: 552 939 812 355 1398 3952
Fragments arranged by size:
3952 1398 939 812 552 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7269
Fragments arranged by size:
7269 683 56
BtsI GCAGTG_nn'
Cuts at: 4014 4862 5524 6318 6338 4014
Size: 848 662 794 20 5684
Fragments arranged by size:
5684 848 794 662 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7973
DraI TTT'AAA
Cuts at: 3436 4158 4800 5259 5312 3436
Size: 722 642 459 53 6132
Fragments arranged by size:
6132 722 642 459 53
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7015 809
Size: 507 5699 1802
Fragments arranged by size:
5699 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4371 5455 6344
Size: 54 1425 50 275 18 2541 1084 889
Cuts at: 6344 7973 8
Size: 1629 43
Fragments arranged by size:
2541 1629 1425 1084 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5455 1537
Size: 275 3643 4090
Fragments arranged by size:
4090 3643 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 2484 2880 3093 3274 4185 4191 4512
Size: 989 142 396 213 181 911 6 321
Cuts at: 4512 5938 1353
Size: 1426 3423
Fragments arranged by size:
3423 1426 989 911 396 321 213 181
142 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6595 6969 7115 387
Size: 1064 138 5006 374 146 1280
Fragments arranged by size:
5006 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2060 2060
Size: 8008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3053 3176 4073 4644 5009 6071 6668
Size: 393 1421 123 897 571 365 1062 597
Cuts at: 6668 7455 7723 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1421 1062 897 787 597 571 393
365 268 123
EcoNI CCTnn'n_nnAGG
Cuts at: 3578 3578
Size: 8008
EcoO109I rG'GnC_Cy
Cuts at: 2086 2086
Size: 8008
EcoRI G'AATT_C
Cuts at: 2104 4914 5317 5695 2104
Size: 2810 403 378 4417
Fragments arranged by size:
4417 2810 403 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4265 4265
Size: 8008
FspI TGC'GCA
Cuts at: 6514 6514
Size: 8008
HaeII r_GCGC'y
Cuts at: 1678 5284 7155 1678
Size: 3606 1871 2531
Fragments arranged by size:
3606 2531 1871
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2063 2095 4495
Size: 32 135 32 445 32 14 32 2400
Cuts at: 4495 4527 6649 6681 6723 6755 1373
Size: 32 2122 32 42 32 2626
Fragments arranged by size:
2626 2400 2122 445 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 4713 5352 5598 669
Size: 988 281 93 2682 639 246 3079
Fragments arranged by size:
3079 2682 988 639 281 246 93
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8008
HpaI GTT'AAC
Cuts at: 4713 5598 4713
Size: 885 7123
KasI G'GCGC_C
Cuts at: 5280 5280
Size: 8008
KpnI G_GTAC'C
Cuts at: 5333 5333
Size: 8008
MfeI C'AATT_G
Cuts at: 2610 4668 5607 2610
Size: 2058 939 5011
Fragments arranged by size:
5011 2058 939
MluI A'CGCG_T
Cuts at: 7884 7884
Size: 8008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2221 2536 7122 7306 1305
Size: 756 160 315 4586 184 2007
Fragments arranged by size:
4586 2007 756 315 184 160
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6186
Fragments arranged by size:
6186 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3762 4977 5966 6325 6484 518
Size: 648 1008 1588 1215 989 359 159 2042
Fragments arranged by size:
2042 1588 1215 1008 989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2125 5424 6101 7249 7494 1606
Size: 513 6 3299 677 1148 245 2120
Fragments arranged by size:
3299 2120 1148 677 513 245 6
NarI GG'CG_CC
Cuts at: 5281 5281
Size: 8008
NcoI C'CATG_G
Cuts at: 513 1065 2004 2816 4569 513
Size: 552 939 812 1753 3952
Fragments arranged by size:
3952 1753 939 812 552
NdeI CA'TA_TG
Cuts at: 387 2194 3637 5232 387
Size: 1807 1443 1595 3163
Fragments arranged by size:
3163 1807 1595 1443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8008
NotI GC'GGCC_GC
Cuts at: 5455 5455
Size: 8008
NspI r_CATG'y
Cuts at: 1132 1229 2928 5366 6911 1132
Size: 97 1699 2438 1545 2229
Fragments arranged by size:
2438 2229 1699 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8008
PciI A'CATG_T
Cuts at: 2924 6907 2924
Size: 3983 4025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8008
PfoI T'CCnGG_A
Cuts at: 2215 2215
Size: 8008
PmeI GTTT'AAAC
Cuts at: 5312 5312
Size: 8008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2373 6077 6109 7590 7622 2341
Size: 32 3704 32 1481 32 2727
Fragments arranged by size:
3704 2727 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 2086
Size: 8008
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8008
PsiI TTA'TAA
Cuts at: 5578 5578
Size: 8008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2850 2882 3798 3830 2850
Size: 32 916 32 7028
Fragments arranged by size:
7028 916 32 32
PstI C_TGCA'G
Cuts at: 830 5360 830
Size: 4530 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8008
PvuII CAG'CTG
Cuts at: 2119 2119
Size: 8008
SacI G_AGCT'C
Cuts at: 2062 2062
Size: 8008
SalI G'TCGA_C
Cuts at: 1936 5350 1936
Size: 3414 4594
SapI GCTCTTCn'nnn_
Cuts at: 3093 4191 3093
Size: 1098 6910
SbfI CC_TGCA'GG
Cuts at: 5360 5360
Size: 8008
ScaI AGT'ACT
Cuts at: 4945 6256 7968 4945
Size: 1311 1712 4985
Fragments arranged by size:
4985 1712 1311
SfcI C'TryA_G
Cuts at: 826 1045 2711 3774 4329 5356 6491 7172 7363
Size: 219 1666 1063 555 1027 1135 681 191
Cuts at: 7363 826
Size: 1471
Fragments arranged by size:
1666 1471 1135 1063 1027 681 555 219
191
SfoI GGC'GCC
Cuts at: 5282 5282
Size: 8008
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8008
SmaI CCC'GGG
Cuts at: 1872 5335 1872
Size: 3463 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2656 2678 3529 4097 4512 6112
Size: 197 926 713 22 851 568 415 1600
Cuts at: 6112 7013 7275 820
Size: 901 262 1553
Fragments arranged by size:
1600 1553 926 901 851 713 568 415
262 197 22
SnaBI TAC'GTA
Cuts at: 493 5799 493
Size: 5306 2702
SpeI A'CTAG_T
Cuts at: 152 2863 152
Size: 2711 5297
SphI G_CATG'C
Cuts at: 1132 5366 1132
Size: 4234 3774
SspI AAT'ATT
Cuts at: 5 52 2512 5932 5
Size: 47 2460 3420 2081
Fragments arranged by size:
3420 2460 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8008
StyI C'CwwG_G
Cuts at: 513 1065 2004 2287 2816 4569 5447 513
Size: 552 939 283 529 1753 878 3074
Fragments arranged by size:
3074 1753 939 878 552 529 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2809 2889 3123 4481 6035 6052 6205
Size: 1255 1229 80 234 1358 1554 17 153
Cuts at: 6205 6390 6835 325
Size: 185 445 1498
Fragments arranged by size:
1554 1498 1358 1255 1229 445 234 185
153 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4283 4943 6254
Size: 274 80 33 51 1443 2306 660 1311
Cuts at: 6254 7966 96
Size: 1712 138
Fragments arranged by size:
2306 1712 1443 1311 660 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5154 5186 7968 8000 5154
Size: 32 2782 32 5162
Fragments arranged by size:
5162 2782 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8008
XbaI T'CTAG_A
Cuts at: 2360 3295 5344 2360
Size: 935 2049 5024
Fragments arranged by size:
5024 2049 935
XhoI C'TCGA_G
Cuts at: 1943 2678 1943
Size: 735 7273
XmaI C'CCGG_G
Cuts at: 1870 5333 1870
Size: 3463 4545
XmnI GAAnn'nnTTC
Cuts at: 2835 4996 6137 2835
Size: 2161 1141 4706
Fragments arranged by size:
4706 2161 1141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2028 5816 276
Size: 53 83 186 936 494 3788 2468
Fragments arranged by size:
3788 2468 936 494 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI