Restriction map for
pFN21AA0591
(Circular) (Six-base) MAPSORT of: pFN21AA0591.seq Check: 4833 from: 1 to: 10153
pFN21AA0591 10153 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3371 7963 278
Size: 53 83 186 936 1835 4592 2468
Fragments arranged by size:
4592 2468 1835 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 6921 7496 1937
Size: 1464 3520 575 4594
Fragments arranged by size:
4594 3520 1464 575
Acc65I G'GTAC_C
Cuts at: 7474 7474
Size: 10153
AclI AA'CG_TT
Cuts at: 1563 3430 8280 8653 1563
Size: 1867 4850 373 3063
Fragments arranged by size:
4850 3063 1867 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2137 3161 4209 4855 5337 6056 8216 9600
Size: 505 1024 1048 646 482 719 2160 1384
Cuts at: 9600 9868 1632
Size: 268 1917
Fragments arranged by size:
2160 1917 1384 1048 1024 719 646 505
482 268
AfeI AGC'GCT
Cuts at: 3809 3809
Size: 10153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9956
AflIII A'CryG_T
Cuts at: 1203 9052 10029 1203
Size: 7849 977 1327
Fragments arranged by size:
7849 1327 977
AgeI A'CCGG_T
Cuts at: 8984 8984
Size: 10153
AhdI GACnn_n'nnGTC
Cuts at: 5143 8882 5143
Size: 3739 6414
AleI CACnn'nnGTG
Cuts at: 1166 4698 1166
Size: 3532 6621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2261 2319 5535 1184
Size: 1077 58 3216 5802
Fragments arranged by size:
5802 3216 1077 58
AlwNI CAG_nnn'CTG
Cuts at: 3166 3692 4117 4549 6822 7155 7896 9468 3166
Size: 526 425 432 2273 333 741 1572 3851
Fragments arranged by size:
3851 2273 1572 741 526 432 425 333
ApaLI G'TGCA_C
Cuts at: 6803 8210 9366 6803
Size: 1407 1156 7590
Fragments arranged by size:
7590 1407 1156
ApoI r'AATT_y
Cuts at: 1493 2448 2956 4232 5079 6791 6992 7058 7462
Size: 955 508 1276 847 1712 201 66 404
Cuts at: 7462 7688 7840 9949 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 1276 955 847 508 404
226 201 152 66
AseI AT'TA_AT
Cuts at: 160 1032 2817 5253 8707 160
Size: 872 1785 2436 3454 1606
Fragments arranged by size:
3454 2436 1785 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10153
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 6234 6969 7041 7435 7478 1870
Size: 73 112 4179 735 72 394 43 4545
Fragments arranged by size:
4545 4179 735 394 112 73 72 43
AvrII C'CTAG_G
Cuts at: 3571 3571
Size: 10153
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6393 6426 6393
Size: 33 10120
BamHI G'GATC_C
Cuts at: 6856 7483 7850 6856
Size: 627 367 9159
Fragments arranged by size:
9159 627 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 3699 7474 8929 618
Size: 325 217 627 993 919 3775 1455 1842
Fragments arranged by size:
3775 1842 1455 993 919 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3002 3727 3801 4682 5466 5922 6507 7472
Size: 1611 725 74 881 784 456 585 965
Cuts at: 7472 1391
Size: 4072
Fragments arranged by size:
4072 1611 965 881 784 725 585 456
74
BbeI G_GCGC'C
Cuts at: 3703 3703
Size: 10153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3627 3947 6033 6217 6956 7186 7298
Size: 971 1728 320 2086 184 739 230 112
Cuts at: 7298 928
Size: 3783
Fragments arranged by size:
3783 2086 1728 971 739 320 230 184
112
BbvCI CC'TCA_GC
Cuts at: 5643 6605 6620 5643
Size: 962 15 9176
Fragments arranged by size:
9176 962 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4697 4731 8344 8378 1903
Size: 34 2760 34 3613 34 3678
Fragments arranged by size:
3678 3613 2760 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4968 7115 8045 9255 4968
Size: 2147 930 1210 5866
Fragments arranged by size:
5866 2147 1210 930
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10153
BfrBI ATG'CAT
Cuts at: 2082 2082
Size: 10153
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2352 4250 6279
Size: 107 122 71 631 605 680 1898 2029
Cuts at: 6279 8764 136
Size: 2485 1525
Fragments arranged by size:
2485 2029 1898 1525 680 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4062 4093 4864 6144 6246 6648 10148 4062
Size: 31 771 1280 102 402 3500 4067
Fragments arranged by size:
4067 3500 1280 771 402 102 31
BlpI GC'TnA_GC
Cuts at: 3693 3716 3930 7570 3693
Size: 23 214 3640 6276
Fragments arranged by size:
6276 3640 214 23
Bme1580I G_kGCm'C
Cuts at: 1790 4603 4968 6807 8214 9370 1790
Size: 2813 365 1839 1407 1156 2573
Fragments arranged by size:
2813 2573 1839 1407 1156 365
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5351 7165 7884
Size: 416 53 83 399 24 3934 1814 719
Cuts at: 7884 8842 442
Size: 958 1753
Fragments arranged by size:
3934 1814 1753 958 719 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3269 1056
Size: 2213 7940
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3741 3979 4609 6482 6954 3741
Size: 238 630 1873 472 6940
Fragments arranged by size:
6940 1873 630 472 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3429 4579 5217 5807 6512 6521 8813 1239
Size: 2190 1150 638 590 705 9 2292 2579
Fragments arranged by size:
2579 2292 2190 1150 705 638 590 9
Bpu10I CC'TnA_GC
Cuts at: 3159 3862 4943 5643 6581 6605 6620 3159
Size: 703 1081 700 938 24 15 6692
Fragments arranged by size:
6692 1081 938 703 700 24 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4523 6494 8278 9143 9441 4523
Size: 1971 1784 865 298 5235
Fragments arranged by size:
5235 1971 1784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 2181 2696 3950 7114 8816 882
Size: 617 555 127 515 1254 3164 1702 2219
Fragments arranged by size:
3164 2219 1702 1254 617 555 515 127
BsaAI yAC'GTr
Cuts at: 493 1204 3823 7944 493
Size: 711 2619 4121 2702
Fragments arranged by size:
4121 2702 2619 711
BsaBI GATnn'nnATC
Cuts at: 3186 6309 10147 3186
Size: 3123 3838 3192
Fragments arranged by size:
3838 3192 3123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3368 3700 4830 7960
Size: 53 83 186 936 1835 332 1130 3130
Cuts at: 7960 8342 8977 275
Size: 382 635 1451
Fragments arranged by size:
3130 1835 1451 1130 936 635 382 332
186 83 53
BsaWI w'CCGG_w
Cuts at: 3753 4257 8586 8984 9258 9405 9816 3753
Size: 504 4329 398 274 147 411 4090
Fragments arranged by size:
4329 4090 504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 2531 2561 6877
Size: 30 165 30 601 30 497 30 4316
Cuts at: 6877 6907 1178
Size: 30 4424
Fragments arranged by size:
4424 4316 601 497 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3140 3426 4208 4868 5195 5220 6631 6889
Size: 1954 286 782 660 327 25 1411 258
Cuts at: 6889 1186
Size: 4450
Fragments arranged by size:
4450 1954 1411 782 660 327 286 258
25
BseYI C'CCAG_C
Cuts at: 2597 3843 5809 6599 7015 9356 9852 2597
Size: 1246 1966 790 416 2341 496 2898
Fragments arranged by size:
2898 2341 1966 1246 790 496 416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2860 4385 5531 7300 1163
Size: 573 1124 1525 1146 1769 4016
Fragments arranged by size:
4016 1769 1525 1146 1124 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4723 4752 4900 7603 8364 9392
Size: 275 186 2722 29 148 2703 761 1028
Cuts at: 9392 1540
Size: 2301
Fragments arranged by size:
2722 2703 2301 1028 761 275 186 148
29
BsiHKAI G_wGCw'C
Cuts at: 3035 3160 4682 5922 6480 6507 6807 8214 8299
Size: 125 1522 1240 558 27 300 1407 85
Cuts at: 8299 9370 3035
Size: 1071 3818
Fragments arranged by size:
3818 1522 1407 1240 1071 558 300 125
85 27
BsmI GAATG_Cn'
Cuts at: 4817 5450 7664 7757 4817
Size: 633 2214 93 7213
Fragments arranged by size:
7213 2214 633 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3002 3035 3160 3727 3801 4603 4682
Size: 399 1212 33 125 567 74 802 79
Cuts at: 4682 4968 5466 5922 6480 6507 6807 7472 8214
Size: 286 498 456 558 27 300 665 742
Cuts at: 8214 8299 9370 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1212 1071 802 742 665 567 558
498 456 399 300 286 125 85 79
74 33 27
BspEI T'CCGG_A
Cuts at: 3753 4257 9816 3753
Size: 504 5559 4090
Fragments arranged by size:
5559 4090 504
BspHI T'CATG_A
Cuts at: 4447 8042 9772 4447
Size: 3595 1730 4828
Fragments arranged by size:
4828 3595 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4144 7508 844
Size: 3300 3364 3489
Fragments arranged by size:
3489 3364 3300
BsrBI CCG'CTC
Cuts at: 1394 1823 3697 3720 8040 1394
Size: 429 1874 23 4320 3507
Fragments arranged by size:
4320 3507 1874 429 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 2988 3079 3453 4315 5160 6181
Size: 1183 1329 411 91 374 862 845 1021
Cuts at: 6181 8648 8822 65
Size: 2467 174 1396
Fragments arranged by size:
2467 1396 1329 1183 1021 862 845 411
374 174 91
BsrFI r'CCGG_y
Cuts at: 1700 1810 4832 6193 8797 8984 1700
Size: 110 3022 1361 2604 187 2869
Fragments arranged by size:
3022 2869 2604 1361 187 110
BsrGI T'GTAC_A
Cuts at: 96 2223 5032 96
Size: 2127 2809 5217
Fragments arranged by size:
5217 2809 2127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10153
BssSI C'ACGA_G
Cuts at: 3924 8213 9225 3924
Size: 4289 1012 4852
Fragments arranged by size:
4852 4289 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2987 3053 4144 5996 794
Size: 2193 66 1091 1852 4951
Fragments arranged by size:
4951 2193 1852 1091 66
BstBI TT'CG_AA
Cuts at: 6308 8957 6308
Size: 2649 7504
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10153
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 3511 4345 4435 2617
Size: 894 834 90 8335
Fragments arranged by size:
8335 894 834 90
BstYI r'GATC_y
Cuts at: 1972 2194 2542 2746 4062 4093 4864 6144 6246
Size: 222 348 204 1316 31 771 1280 102
Cuts at: 6246 6648 6856 7483 7850 8235 8252 9704 9790
Size: 402 208 627 367 385 17 1452 86
Cuts at: 9790 9802 10148 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1316 1280 771 627 402 385
367 348 346 222 208 204 102 86
31 17 12
Bsu36I CC'TnA_GG
Cuts at: 5973 5973
Size: 10153
BtgI C'CryG_G
Cuts at: 513 1065 3003 3309 4693 4746 5457 6454 7284
Size: 552 1938 306 1384 53 711 997 830
Cuts at: 7284 7327 513
Size: 43 3339
Fragments arranged by size:
3339 1938 1384 997 830 711 552 306
53 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5026 5420 487
Size: 683 56 3800 394 5220
Fragments arranged by size:
5220 3800 683 394 56
BtsI GCAGTG_nn'
Cuts at: 5353 5529 6616 6700 6886 7669 8463 8483 5353
Size: 176 1087 84 186 783 794 20 7023
Fragments arranged by size:
7023 1087 794 783 186 176 84 20
ClaI AT'CG_AT
Cuts at: 1554 3198 1554
Size: 1644 8509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6646 6681 7086 7121 529
Size: 35 6082 35 405 35 3561
Fragments arranged by size:
6082 3561 405 35 35 35
DraI TTT'AAA
Cuts at: 3435 7457 3435
Size: 4022 6131
DraIII CAC_nnn'GTG
Cuts at: 2662 4981 2662
Size: 2319 7834
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9160 809
Size: 507 7844 1802
Fragments arranged by size:
7844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2859 3289 3679
Size: 54 1425 50 275 18 1029 430 390
Cuts at: 3679 4749 4983 5734 7600 8489 10118 8
Size: 1070 234 751 1866 889 1629 43
Fragments arranged by size:
1866 1629 1425 1070 1029 889 751 430
390 275 234 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4749 7600 1537
Size: 275 2937 2851 4090
Fragments arranged by size:
4090 2937 2851 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3474 3732 4185 4197 4874 5313 6423 8083
Size: 2121 258 453 12 677 439 1110 1660
Cuts at: 8083 1353
Size: 3423
Fragments arranged by size:
3423 2121 1660 1110 677 453 439 258
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5628 8740 9114 9260 387
Size: 1064 138 4039 3112 374 146 1280
Fragments arranged by size:
4039 3112 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4680 5920 6505 4680
Size: 1240 585 8328
Fragments arranged by size:
8328 1240 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 3161 3429 4209 4579 4855 5217
Size: 393 505 1024 268 780 370 276 362
Cuts at: 5217 5337 5807 6056 6512 6521 8216 8813 9600
Size: 120 470 249 456 9 1695 597 787
Cuts at: 9600 9868 1239
Size: 268 1524
Fragments arranged by size:
1695 1524 1024 787 780 597 505 470
456 393 370 362 276 268 268 249
120 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4325 6512 7340 4325
Size: 2187 828 7138
Fragments arranged by size:
7138 2187 828
EcoO109I rG'GnC_Cy
Cuts at: 2043 3091 3145 3163 3315 6060 6615 7333 7345
Size: 1048 54 18 152 2745 555 718 12
Cuts at: 7345 2043
Size: 4851
Fragments arranged by size:
4851 2745 1048 718 555 152 54 18
12
EcoRI G'AATT_C
Cuts at: 5079 6791 7462 7840 5079
Size: 1712 671 378 7392
Fragments arranged by size:
7392 1712 671 378
EcoRV GAT'ATC
Cuts at: 3010 5410 6838 3010
Size: 2400 1428 6325
Fragments arranged by size:
6325 2400 1428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3503 4112 7142 3503
Size: 609 3030 6514
Fragments arranged by size:
6514 3030 609
FspI TGC'GCA
Cuts at: 8659 8659
Size: 10153
HaeII r_GCGC'y
Cuts at: 1678 3703 3811 4714 6675 9300 1678
Size: 2025 108 903 1961 2625 2531
Fragments arranged by size:
2625 2531 2025 1961 903 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 2531 2563 3191
Size: 32 135 32 687 32 240 32 628
Cuts at: 3191 3223 3586 3618 4820 4844 4851 4852 4876
Size: 32 363 32 1202 24 7 1 24
Cuts at: 4876 4883 5057 5089 5129 5161 5255 5287 5790
Size: 7 174 32 40 32 94 32 503
Cuts at: 5790 5822 6129 6161 6578 6610 8794 8826 8868
Size: 32 307 32 417 32 2184 32 42
Cuts at: 8868 8900 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 1202 687 628 503 417 363
307 240 174 135 94 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 32 24 24 7
7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3610 5821 7497 7743 669
Size: 988 281 1672 2211 1676 246 3079
Fragments arranged by size:
3079 2211 1676 1672 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3485 7418 748
Size: 2737 3933 3483
Fragments arranged by size:
3933 3483 2737
HpaI GTT'AAC
Cuts at: 3610 7743 3610
Size: 4133 6020
KasI G'GCGC_C
Cuts at: 3699 3699
Size: 10153
KpnI G_GTAC'C
Cuts at: 7478 7478
Size: 10153
MfeI C'AATT_G
Cuts at: 6846 7752 6846
Size: 906 9247
MluI A'CGCG_T
Cuts at: 10029 10029
Size: 10153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 9267 9451 1305
Size: 3477 4485 184 2007
Fragments arranged by size:
4485 3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3291 3681 4985 5736 10
Size: 54 1425 343 1459 390 1304 751 4427
Fragments arranged by size:
4427 1459 1425 1304 751 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2368 2697 3509 4343 4433 4698
Size: 648 924 278 329 812 834 90 265
Cuts at: 4698 4919 5439 8111 8470 8629 518
Size: 221 520 2672 359 159 2042
Fragments arranged by size:
2672 2042 924 834 812 648 520 359
329 278 265 221 159 90
MspA1I CmG'CkG
Cuts at: 1606 3005 3774 3856 5048 5813 6285 7019 7569
Size: 1399 769 82 1192 765 472 734 550
Cuts at: 7569 8246 9394 9639 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1399 1192 1148 769 765 734 677
550 472 245 82
NaeI GCC'GGC
Cuts at: 4834 6195 4834
Size: 1361 8792
NarI GG'CG_CC
Cuts at: 3700 3700
Size: 10153
NcoI C'CATG_G
Cuts at: 513 1065 3309 7284 513
Size: 552 2244 3975 3382
Fragments arranged by size:
3975 3382 2244 552
NdeI CA'TA_TG
Cuts at: 387 6415 387
Size: 6028 4125
NgoMIV G'CCGG_C
Cuts at: 4832 6193 4832
Size: 1361 8792
NheI G'CTAG_C
Cuts at: 1052 3265 1052
Size: 2213 7940
NotI GC'GGCC_GC
Cuts at: 7600 7600
Size: 10153
NsiI A_TGCA'T
Cuts at: 2084 2084
Size: 10153
NspI r_CATG'y
Cuts at: 1132 1229 7511 9056 1132
Size: 97 6282 1545 2229
Fragments arranged by size:
6282 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10153
PciI A'CATG_T
Cuts at: 9052 9052
Size: 10153
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 4981 1108
Size: 1417 2456 6280
Fragments arranged by size:
6280 2456 1417
PfoI T'CCnGG_A
Cuts at: 4491 4491
Size: 10153
PmeI GTTT'AAAC
Cuts at: 7457 7457
Size: 10153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8222 8254 9735 9767 8222
Size: 32 1481 32 8608
Fragments arranged by size:
8608 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3091 3145 3315 6060 6615 7333 3091
Size: 54 170 2745 555 718 5911
Fragments arranged by size:
5911 2745 718 555 170 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10153
PsiI TTA'TAA
Cuts at: 7723 7723
Size: 10153
PstI C_TGCA'G
Cuts at: 830 4180 5992 7505 830
Size: 3350 1812 1513 3478
Fragments arranged by size:
3478 3350 1812 1513
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10153
PvuII CAG'CTG
Cuts at: 3856 5813 7019 3856
Size: 1957 1206 6990
Fragments arranged by size:
6990 1957 1206
SacI G_AGCT'C
Cuts at: 4682 5922 6507 4682
Size: 1240 585 8328
Fragments arranged by size:
8328 1240 585
SacII CC_GC'GG
Cuts at: 3006 3006
Size: 10153
SalI G'TCGA_C
Cuts at: 1936 7495 1936
Size: 5559 4594
SanDI GG'GwC_CC
Cuts at: 3315 6615 3315
Size: 3300 6853
SapI GCTCTTCn'nnn_
Cuts at: 3732 3732
Size: 10153
SbfI CC_TGCA'GG
Cuts at: 7505 7505
Size: 10153
ScaI AGT'ACT
Cuts at: 8401 10113 8401
Size: 1712 8441
SfcI C'TryA_G
Cuts at: 826 1045 2427 2920 4049 4176 5793 5988 7501
Size: 219 1382 493 1129 127 1617 195 1513
Cuts at: 7501 8636 9317 9508 826
Size: 1135 681 191 1471
Fragments arranged by size:
1617 1513 1471 1382 1135 1129 681 493
219 195 191 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 4250 4250
Size: 10153
SfoI GGC'GCC
Cuts at: 3701 3701
Size: 10153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10153
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10153
SmaI CCC'GGG
Cuts at: 1872 6971 7480 1872
Size: 5099 509 4545
Fragments arranged by size:
5099 4545 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2055 4538 6473 8257 9158 9420
Size: 197 926 112 2483 1935 1784 901 262
Cuts at: 9420 820
Size: 1553
Fragments arranged by size:
2483 1935 1784 1553 926 901 262 197
112
SnaBI TAC'GTA
Cuts at: 493 3823 7944 493
Size: 3330 4121 2702
Fragments arranged by size:
4121 3330 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10153
SphI G_CATG'C
Cuts at: 1132 7511 1132
Size: 6379 3774
SspI AAT'ATT
Cuts at: 5 52 2815 5518 6016 8077 5
Size: 47 2763 2703 498 2061 2081
Fragments arranged by size:
2763 2703 2081 2061 498 47
StuI AGG'CCT
Cuts at: 1517 4142 4459 1517
Size: 2625 317 7211
Fragments arranged by size:
7211 2625 317
StyI C'CwwG_G
Cuts at: 513 1065 2856 3309 3571 4217 4907 5175 5731
Size: 552 1791 453 262 646 690 268 556
Cuts at: 5731 6207 7284 7592 513
Size: 476 1077 308 3074
Fragments arranged by size:
3074 1791 1077 690 646 556 552 476
453 308 268 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2532 2696 4914 5619 5686 6747 6788
Size: 1255 952 164 2218 705 67 1061 41
Cuts at: 6788 8180 8197 8350 8535 8980 325
Size: 1392 17 153 185 445 1498
Fragments arranged by size:
2218 1498 1392 1255 1061 952 705 445
185 164 153 67 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2932 4398
Size: 274 80 33 51 1443 246 709 1466
Cuts at: 4398 5032 7263 8399 10111 96
Size: 634 2231 1136 1712 138
Fragments arranged by size:
2231 1712 1466 1443 1136 709 634 274
246 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2520 2552 6349 6381 6877 6909 10113
Size: 32 484 32 3797 32 496 32 3204
Cuts at: 10113 10145 2004
Size: 32 2012
Fragments arranged by size:
3797 3204 2012 496 484 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5205 6157 1316
Size: 3889 952 5312
Fragments arranged by size:
5312 3889 952
XbaI T'CTAG_A
Cuts at: 7489 7489
Size: 10153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3103 3307 7255 3103
Size: 204 3948 6001
Fragments arranged by size:
6001 3948 204
XhoI C'TCGA_G
Cuts at: 1943 2055 1943
Size: 112 10041
XmaI C'CCGG_G
Cuts at: 1870 6969 7478 1870
Size: 5099 509 4545
Fragments arranged by size:
5099 4545 509
XmnI GAAnn'nnTTC
Cuts at: 4212 4422 7002 8282 4212
Size: 210 2580 1280 6083
Fragments arranged by size:
6083 2580 1280 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3369 7961 276
Size: 53 83 186 936 1835 4592 2468
Fragments arranged by size:
4592 2468 1835 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BsiWI BsmBI BstZ17I FseI
FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SexAI SrfI SwaI