Restriction map for
pFN21AA0589
(Circular) (Six-base) MAPSORT of: pFN21AA0589.seq Check: 6918 from: 1 to: 6709
pFN21AA0589 6709 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3727 3880 3727
Size: 153 6556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3635 4519 278
Size: 53 83 186 936 2099 884 2468
Fragments arranged by size:
2468 2099 936 884 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4052 1937
Size: 2115 4594
Acc65I G'GTAC_C
Cuts at: 2017 4030 2017
Size: 2013 4696
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2872 3374 4772 6156 6424 1632
Size: 629 611 502 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 629 611 502 268
AfeI AGC'GCT
Cuts at: 2038 3305 2038
Size: 1267 5442
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 5608 6585 1203
Size: 4405 977 1327
Fragments arranged by size:
4405 1327 977
AgeI A'CCGG_T
Cuts at: 5540 5540
Size: 6709
AhdI GACnn_n'nnGTC
Cuts at: 3271 5438 3271
Size: 2167 4542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 6677
AlwNI CAG_nnn'CTG
Cuts at: 2605 4452 6024 2605
Size: 1847 1572 3290
Fragments arranged by size:
3290 1847 1572
ApaI G_GGCC'C
Cuts at: 2159 3417 3487 2159
Size: 1258 70 5381
Fragments arranged by size:
5381 1258 70
ApaLI G'TGCA_C
Cuts at: 2972 4766 5922 2972
Size: 1794 1156 3759
Fragments arranged by size:
3759 1794 1156
ApoI r'AATT_y
Cuts at: 1493 4018 4244 4396 6505 1493
Size: 2525 226 152 2109 1697
Fragments arranged by size:
2525 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2788 2788
Size: 6709
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2512 2858 4034 1870
Size: 73 569 346 1176 4545
Fragments arranged by size:
4545 1176 569 346 73
BamHI G'GATC_C
Cuts at: 2451 3141 4039 4406 2451
Size: 690 898 367 4754
Fragments arranged by size:
4754 898 690 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2246 2516 3014 3095
Size: 325 217 627 230 229 270 498 81
Cuts at: 3095 3812 4030 5485 618
Size: 717 218 1455 1842
Fragments arranged by size:
1842 1455 717 627 498 325 270 230
229 218 217 81
BanII G_rGCy'C
Cuts at: 1391 2159 2291 2445 2774 2868 3417 3487 4028
Size: 768 132 154 329 94 549 70 541
Cuts at: 4028 1391
Size: 4072
Fragments arranged by size:
4072 768 549 541 329 154 132 94
70
BbeI G_GCGC'C
Cuts at: 2520 3018 3099 2520
Size: 498 81 6130
Fragments arranged by size:
6130 498 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2228 928
Size: 971 329 5409
Fragments arranged by size:
5409 971 329
BbvCI CC'TCA_GC
Cuts at: 2040 2040
Size: 6709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4900 4934 1903
Size: 34 2963 34 3678
Fragments arranged by size:
3678 2963 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4601 5811 4601
Size: 1210 5499
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2863 3431 3848
Size: 107 122 71 631 605 1191 568 417
Cuts at: 3848 3881 3983 5320 136
Size: 33 102 1337 1525
Fragments arranged by size:
1525 1337 1191 631 605 568 417 122
107 102 71 33
BglII A'GATC_T
Cuts at: 6704 6704
Size: 6709
BlpI GC'TnA_GC
Cuts at: 4126 4126
Size: 6709
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2159 2976 3417 3487 4770 5926 1790
Size: 268 101 817 441 70 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 817 441 268 101 70
BmgBI CAC'GTC
Cuts at: 1318 2308 1318
Size: 990 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3460 4440 5398
Size: 416 53 83 399 24 2043 980 958
Cuts at: 5398 442
Size: 1753
Fragments arranged by size:
2043 1753 980 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3398 1056
Size: 2342 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 3251 3524 5369 1239
Size: 794 1218 273 1845 2579
Fragments arranged by size:
2579 1845 1218 794 273
Bpu10I CC'TnA_GC
Cuts at: 2040 2285 3612 2040
Size: 245 1327 5137
Fragments arranged by size:
5137 1327 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2750 4834 5699 5997 2738
Size: 12 2084 865 298 3450
Fragments arranged by size:
3450 2084 865 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5372 882
Size: 617 3873 2219
Fragments arranged by size:
3873 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 4500 493
Size: 711 1125 2171 2702
Fragments arranged by size:
2702 2171 1125 711
BsaBI GATnn'nnATC
Cuts at: 6703 6703
Size: 6709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 2877 3015 3096
Size: 53 83 186 936 984 360 138 81
Cuts at: 3096 3632 4516 4898 5533 275
Size: 536 884 382 635 1451
Fragments arranged by size:
1451 984 936 884 635 536 382 360
186 138 83 81 53
BsaWI w'CCGG_w
Cuts at: 2020 5142 5540 5814 5961 6372 2020
Size: 3122 398 274 147 411 2357
Fragments arranged by size:
3122 2357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 2273 2303 2459
Size: 30 165 30 651 30 189 30 156
Cuts at: 2459 2489 2576 2606 3452 3482 3734 3764 1178
Size: 30 87 30 846 30 252 30 4123
Fragments arranged by size:
4123 846 651 252 189 165 156 87
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 3337 3473 3710 3794 3875 3908 3952
Size: 1035 1116 136 237 84 81 33 44
Cuts at: 3952 1186
Size: 3943
Fragments arranged by size:
3943 1116 1035 237 136 84 81 44
33
BseYI C'CCAG_C
Cuts at: 2257 2351 2965 3287 3409 3942 5912 6408 2257
Size: 94 614 322 122 533 1970 496 2558
Fragments arranged by size:
2558 1970 614 533 496 322 122 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2702 3164 3520 3755 3772 1163
Size: 573 966 462 356 235 17 4100
Fragments arranged by size:
4100 966 573 462 356 235 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3810 4159 4920 5948 1540
Size: 275 186 1159 650 349 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 650 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2291 2313 2445 2976 3026 3237 4770 4855 5926
Size: 22 132 531 50 211 1533 85 1071
Cuts at: 5926 2291
Size: 3074
Fragments arranged by size:
3074 1533 1071 531 211 132 85 50
22
BsmI GAATG_Cn'
Cuts at: 4220 4313 4220
Size: 93 6616
BsmBI CGTCTCn'nnnn_
Cuts at: 3623 3623
Size: 6709
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2159 2291 2313 2445 2774 2868
Size: 399 268 101 132 22 132 329 94
Cuts at: 2868 2976 3026 3237 3417 3487 4028 4770 4855
Size: 108 50 211 180 70 541 742 85
Cuts at: 4855 5926 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 541 399 329 268 211
180 132 132 108 101 94 85 70
50 22
BspEI T'CCGG_A
Cuts at: 6372 6372
Size: 6709
BspHI T'CATG_A
Cuts at: 2715 3318 4598 6328 2715
Size: 603 1280 1730 3096
Fragments arranged by size:
3096 1730 1280 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2087 3483 3727 3880 4064 844
Size: 1243 1396 244 153 184 3489
Fragments arranged by size:
3489 1396 1243 244 184 153
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 3001 3169 3454 3616 3958 4596
Size: 429 262 916 168 285 162 342 638
Cuts at: 4596 1394
Size: 3507
Fragments arranged by size:
3507 916 638 429 342 285 262 168
162
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 3573 5204 5378 65
Size: 1183 1243 1082 1631 174 1396
Fragments arranged by size:
1631 1396 1243 1183 1082 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3516 3809 5353 5540 1700
Size: 110 1706 293 1544 187 2869
Fragments arranged by size:
2869 1706 1544 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6709
BssHII G'CGCG_C
Cuts at: 1925 2788 3101 1925
Size: 863 313 5533
Fragments arranged by size:
5533 863 313
BssSI C'ACGA_G
Cuts at: 2990 4769 5781 2990
Size: 1779 1012 3918
Fragments arranged by size:
3918 1779 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 2854 794
Size: 1811 249 4649
Fragments arranged by size:
4649 1811 249
BstBI TT'CG_AA
Cuts at: 3552 5513 3552
Size: 1961 4748
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6709
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2257 3076 2011
Size: 246 819 5644
Fragments arranged by size:
5644 819 246
BstYI r'GATC_y
Cuts at: 1972 2451 3141 3441 3715 4039 4406 4791 4808
Size: 479 690 300 274 324 367 385 17
Cuts at: 4808 6260 6346 6358 6704 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 690 479 385 367 346 324
300 274 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 2274 2642 3069 3075 3160
Size: 552 939 160 110 368 427 6 85
Cuts at: 3160 3644 3705 513
Size: 484 61 3517
Fragments arranged by size:
3517 939 552 484 427 368 160 110
85 61 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5970
Fragments arranged by size:
5970 683 56
BtsI GCAGTG_nn'
Cuts at: 4225 5019 5039 4225
Size: 794 20 5895
Fragments arranged by size:
5895 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6674
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2924 5716 809
Size: 507 1608 2792 1802
Fragments arranged by size:
2792 1802 1608 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2456 3072
Size: 54 1425 50 275 18 331 295 616
Cuts at: 3072 3157 3432 3807 4156 5045 6674 8
Size: 85 275 375 349 889 1629 43
Fragments arranged by size:
1629 1425 889 616 375 349 331 295
275 275 85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 3807 4156 1537
Size: 275 1345 650 349 4090
Fragments arranged by size:
4090 1345 650 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 2807 3705 4639 1353
Size: 1184 270 898 934 3423
Fragments arranged by size:
3423 1184 934 898 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 3399 5296 5670 5816 387
Size: 1064 138 456 1354 1897 374 146 1280
Fragments arranged by size:
1897 1354 1280 1064 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 2289 2443 2289
Size: 154 6555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2261 2872 3251 3374 3524 4772
Size: 393 401 228 611 379 123 150 1248
Cuts at: 4772 5369 6156 6424 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1248 787 611 597 401 393 379
268 228 150 123
EcoO109I rG'GnC_Cy
Cuts at: 2156 2833 3247 3413 3483 3976 2156
Size: 677 414 166 70 493 4889
Fragments arranged by size:
4889 677 493 414 166 70
EcoRI G'AATT_C
Cuts at: 4018 4396 4018
Size: 378 6331
FseI GG_CCGG'CC
Cuts at: 3520 3520
Size: 6709
FspI TGC'GCA
Cuts at: 5215 5215
Size: 6709
HaeII r_GCGC'y
Cuts at: 1678 2040 2520 2895 3018 3062 3099 3307 3972
Size: 362 480 375 123 44 37 208 665
Cuts at: 3972 5856 1678
Size: 1884 2531
Fragments arranged by size:
2531 1884 665 480 375 362 208 123
44 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 3257 3289 5350
Size: 32 135 32 548 32 1105 32 2061
Cuts at: 5350 5382 5424 5456 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2061 1105 548 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4053 4299 669
Size: 988 281 2115 246 3079
Fragments arranged by size:
3079 2115 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6709
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KasI G'GCGC_C
Cuts at: 2516 3014 3095 2516
Size: 498 81 6130
Fragments arranged by size:
6130 498 81
KpnI G_GTAC'C
Cuts at: 2021 4034 2021
Size: 2013 4696
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 6585 6585
Size: 6709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 3615 4014 5823 6007 1305
Size: 1227 1083 399 1809 184 2007
Fragments arranged by size:
2007 1809 1227 1083 399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 10
Size: 54 1425 343 331 4556
Fragments arranged by size:
4556 1425 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 3074 4667 5026 5185 518
Size: 648 1021 887 1593 359 159 2042
Fragments arranged by size:
2042 1593 1021 887 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2494 2644 2914 3149 3162 3409 3422
Size: 651 237 150 270 235 13 247 13
Cuts at: 3422 3646 3707 4125 4802 5950 6195 1606
Size: 224 61 418 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 418 270 247 245
237 235 224 150 61 13 13
NaeI GCC'GGC
Cuts at: 3518 3518
Size: 6709
NarI GG'CG_CC
Cuts at: 2517 3015 3096 2517
Size: 498 81 6130
Fragments arranged by size:
6130 498 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2164 3075 513
Size: 552 939 160 911 4147
Fragments arranged by size:
4147 939 911 552 160
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6709
NgoMIV G'CCGG_C
Cuts at: 3516 3516
Size: 6709
NheI G'CTAG_C
Cuts at: 1052 3394 1052
Size: 2342 4367
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 2857 4067 5612 1132
Size: 97 1628 1210 1545 2229
Fragments arranged by size:
2229 1628 1545 1210 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6709
PciI A'CATG_T
Cuts at: 5608 5608
Size: 6709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6709
PfoI T'CCnGG_A
Cuts at: 2388 2436 2928 2388
Size: 48 492 6169
Fragments arranged by size:
6169 492 48
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 4778 4810 6291 6323 2576
Size: 32 2170 32 1481 32 2962
Fragments arranged by size:
2962 2170 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 2833
Size: 6709
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6709
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PspOMI G'GGCC_C
Cuts at: 2155 3413 3483 2155
Size: 1258 70 5381
Fragments arranged by size:
5381 1258 70
PstI C_TGCA'G
Cuts at: 830 2601 2913 3207 3723 4061 830
Size: 1771 312 294 516 338 3478
Fragments arranged by size:
3478 1771 516 338 312 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 2257 2257
Size: 6709
SacI G_AGCT'C
Cuts at: 2291 2445 2291
Size: 154 6555
SacII CC_GC'GG
Cuts at: 2645 3163 3647 3708 2645
Size: 518 484 61 5646
Fragments arranged by size:
5646 518 484 61
SalI G'TCGA_C
Cuts at: 1936 4051 1936
Size: 2115 4594
SapI GCTCTTCn'nnn_
Cuts at: 2807 2807
Size: 6709
SbfI CC_TGCA'GG
Cuts at: 4061 4061
Size: 6709
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SfcI C'TryA_G
Cuts at: 826 1045 2597 2909 2953 3203 3211 3564 3719
Size: 219 1552 312 44 250 8 353 155
Cuts at: 3719 3964 4057 5192 5873 6064 826
Size: 245 93 1135 681 191 1471
Fragments arranged by size:
1552 1471 1135 681 353 312 250 245
219 191 155 93 44 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 3983 3983
Size: 6709
SfoI GGC'GCC
Cuts at: 2518 3016 3097 2518
Size: 498 81 6130
Fragments arranged by size:
6130 498 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 2514 4036 1872
Size: 642 1522 4545
Fragments arranged by size:
4545 1522 642
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 2765 4813 5714 5976 820
Size: 197 926 810 12 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 810 262 197 12
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 4067 1132
Size: 2935 3774
SspI AAT'ATT
Cuts at: 5 52 4633 5
Size: 47 4581 2081
Fragments arranged by size:
4581 2081 47
StuI AGG'CCT
Cuts at: 1517 3851 3884 1517
Size: 2334 33 4342
Fragments arranged by size:
4342 2334 33
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 3075 4148 513
Size: 552 939 160 911 1073 3074
Fragments arranged by size:
3074 1073 939 911 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3934 4736 4753 4906 5091 5536 325
Size: 1255 2354 802 17 153 185 445 1498
Fragments arranged by size:
2354 1498 1255 802 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2673 3948
Size: 274 80 33 51 1443 495 201 1275
Cuts at: 3948 4955 6667 96
Size: 1007 1712 138
Fragments arranged by size:
1712 1443 1275 1007 495 274 201 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 2273 2305 3734 3766 6669 6701 2054
Size: 32 187 32 1429 32 2903 32 2062
Fragments arranged by size:
2903 2062 1429 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3586 1316
Size: 2270 4439
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6709
XmaI C'CCGG_G
Cuts at: 1870 2512 4034 1870
Size: 642 1522 4545
Fragments arranged by size:
4545 1522 642
XmnI GAAnn'nnTTC
Cuts at: 2816 4838 2816
Size: 2022 4687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3633 4517 276
Size: 53 83 186 936 2099 884 2468
Fragments arranged by size:
2468 2099 936 884 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SexAI SrfI SwaI XcmI