Restriction map for
pFN21AA0586
(Circular) (Six-base) MAPSORT of: pFN21AA0586.seq Check: 1719 from: 1 to: 9304
pFN21AA0586 9304 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2628 4255 5173 2628
Size: 1627 918 6759
Fragments arranged by size:
6759 1627 918
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7114 278
Size: 53 83 186 936 5578 2468
Fragments arranged by size:
5578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2324 6647 1937
Size: 387 4323 4594
Fragments arranged by size:
4594 4323 387
Acc65I G'GTAC_C
Cuts at: 6625 6625
Size: 9304
AclI AA'CG_TT
Cuts at: 1563 7431 7804 1563
Size: 5868 373 3063
Fragments arranged by size:
5868 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5715 6288 7367 8751 9019 1632
Size: 4083 573 1079 1384 268 1917
Fragments arranged by size:
4083 1917 1384 1079 573 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9107
AflIII A'CryG_T
Cuts at: 1203 2626 6346 6440 8203 9180 1203
Size: 1423 3720 94 1763 977 1327
Fragments arranged by size:
3720 1763 1423 1327 977 94
AgeI A'CCGG_T
Cuts at: 8135 8135
Size: 9304
AhdI GACnn_n'nnGTC
Cuts at: 6580 8033 6580
Size: 1453 7851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4860 5128 1184
Size: 3676 268 5360
Fragments arranged by size:
5360 3676 268
AlwNI CAG_nnn'CTG
Cuts at: 2507 4625 5621 6550 7047 8619 2507
Size: 2118 996 929 497 1572 3192
Fragments arranged by size:
3192 2118 1572 996 929 497
ApaLI G'TGCA_C
Cuts at: 5113 7361 8517 5113
Size: 2248 1156 5900
Fragments arranged by size:
5900 2248 1156
ApoI r'AATT_y
Cuts at: 1493 2460 3111 3367 3609 4614 5867 6196 6613
Size: 967 651 256 242 1005 1253 329 417
Cuts at: 6613 6839 6991 9100 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 1005 967 651 417 329
256 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7858 160
Size: 872 6826 1606
Fragments arranged by size:
6826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9304
AvaI C'yCGr_G
Cuts at: 1870 1943 6530 6629 1870
Size: 73 4587 99 4545
Fragments arranged by size:
4587 4545 99 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2396 2429 6588 6621 2396
Size: 33 4159 33 5079
Fragments arranged by size:
5079 4159 33 33
BamHI G'GATC_C
Cuts at: 4378 6634 7001 4378
Size: 2256 367 6681
Fragments arranged by size:
6681 2256 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4922 5585 6625 8080 618
Size: 325 217 627 3135 663 1040 1455 1842
Fragments arranged by size:
3135 1842 1455 1040 663 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4824 6623 1391
Size: 3433 1799 4072
Fragments arranged by size:
4072 3433 1799
BbeI G_GCGC'C
Cuts at: 4926 4926
Size: 9304
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 6576 928
Size: 971 209 4468 3656
Fragments arranged by size:
4468 3656 971 209
BbvCI CC'TCA_GC
Cuts at: 4386 4386
Size: 9304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4979 5013 7495 7529 1903
Size: 34 50 34 2958 34 2482 34 3678
Fragments arranged by size:
3678 2958 2482 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7196 8406 7196
Size: 1210 8094
BclI T'GATC_A
Cuts at: 1548 2683 3655 1548
Size: 1135 972 7197
Fragments arranged by size:
7197 1135 972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7915 136
Size: 107 122 71 631 605 6243 1525
Fragments arranged by size:
6243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3355 3401 9299 3355
Size: 46 5898 3360
Fragments arranged by size:
5898 3360 46
BlpI GC'TnA_GC
Cuts at: 5538 6721 5538
Size: 1183 8121
Bme1580I G_kGCm'C
Cuts at: 1790 5117 7365 8521 1790
Size: 3327 2248 1156 2573
Fragments arranged by size:
3327 2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2463 4404 6274
Size: 416 53 83 399 24 1046 1941 1870
Cuts at: 6274 7035 7993 442
Size: 761 958 1753
Fragments arranged by size:
1941 1870 1753 1046 958 761 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6556 6556
Size: 9304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2812 4272 4347 4500 4658 4913 5178 5313
Size: 1573 1460 75 153 158 255 265 135
Cuts at: 5313 5535 5657 7964 1239
Size: 222 122 2307 2579
Fragments arranged by size:
2579 2307 1573 1460 265 255 222 158
153 135 122 75
Bpu10I CC'TnA_GC
Cuts at: 4386 5169 5502 4386
Size: 783 333 8188
Fragments arranged by size:
8188 783 333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 5445 7429 8294 8592 2774
Size: 2671 1984 865 298 3486
Fragments arranged by size:
3486 2671 1984 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3985 4997 5418 7967 882
Size: 617 2486 1012 421 2549 2219
Fragments arranged by size:
2549 2486 2219 1012 617 421
BsaAI yAC'GTr
Cuts at: 493 1204 2156 6347 7095 493
Size: 711 952 4191 748 2702
Fragments arranged by size:
4191 2702 952 748 711
BsaBI GATnn'nnATC
Cuts at: 5217 6405 9298 5217
Size: 1188 2893 5223
Fragments arranged by size:
5223 2893 1188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4923 7111 7493 8128
Size: 53 83 186 936 3390 2188 382 635
Cuts at: 8128 275
Size: 1451
Fragments arranged by size:
3390 2188 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7737 8135 8409 8556 8967 7737
Size: 398 274 147 411 8074
Fragments arranged by size:
8074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2515 2545 4245 4275 5308
Size: 30 165 30 1112 30 1700 30 1033
Cuts at: 5308 5311 5338 5341 1178
Size: 3 27 3 5141
Fragments arranged by size:
5141 1700 1112 1033 165 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2815 3450 4374 4476 4479 5316 1186
Size: 1629 635 924 102 3 837 5174
Fragments arranged by size:
5174 1629 924 837 635 102 3
BseYI C'CCAG_C
Cuts at: 5020 5925 5994 8507 9003 5020
Size: 905 69 2513 496 5321
Fragments arranged by size:
5321 2513 905 496 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 2769 4418 1163
Size: 573 831 202 1649 6049
Fragments arranged by size:
6049 1649 831 573 202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6754 7515 8543 1540
Size: 275 186 4753 761 1028 2301
Fragments arranged by size:
4753 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5117 5645 7365 7450 8521 5117
Size: 528 1720 85 1071 5900
Fragments arranged by size:
5900 1720 1071 528 85
BsmI GAATG_Cn'
Cuts at: 2649 6815 6908 2649
Size: 4166 93 5045
Fragments arranged by size:
5045 4166 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4824 5117 5645 6623 7365 7450 8521
Size: 399 3034 293 528 978 742 85 1071
Cuts at: 8521 1391
Size: 2174
Fragments arranged by size:
3034 2174 1071 978 742 528 399 293
85
BspEI T'CCGG_A
Cuts at: 8967 8967
Size: 9304
BspHI T'CATG_A
Cuts at: 2680 3277 5221 7193 8923 2680
Size: 597 1944 1972 1730 3061
Fragments arranged by size:
3061 1972 1944 1730 597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 4255 4390 5173 5436 6659 844
Size: 1784 1627 135 783 263 1223 3489
Fragments arranged by size:
3489 1784 1627 1223 783 263 135
BsrBI CCG'CTC
Cuts at: 1394 1823 7191 1394
Size: 429 5368 3507
Fragments arranged by size:
5368 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2517 5007 7799 7973 65
Size: 1183 1269 2490 2792 174 1396
Fragments arranged by size:
2792 2490 1396 1269 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5101 5582 7948 8135 1700
Size: 110 3291 481 2366 187 2869
Fragments arranged by size:
3291 2869 2366 481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9304
BssSI C'ACGA_G
Cuts at: 7364 8376 7364
Size: 1012 8292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2102 2260 2507 2510 794
Size: 1308 158 247 3 7588
Fragments arranged by size:
7588 1308 247 158 3
BstBI TT'CG_AA
Cuts at: 8108 8108
Size: 9304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9304
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 2836
Size: 9304
BstYI r'GATC_y
Cuts at: 1972 3355 3401 4378 6634 7001 7386 7403 8855
Size: 1383 46 977 2256 367 385 17 1452
Cuts at: 8855 8941 8953 9299 1972
Size: 86 12 346 1977
Fragments arranged by size:
2256 1977 1452 1383 977 385 367 346
86 46 17 12
BstZ17I GTA'TAC
Cuts at: 2325 2325
Size: 9304
BtgI C'CryG_G
Cuts at: 513 1065 2214 2493 5411 513
Size: 552 1149 279 2918 4406
Fragments arranged by size:
4406 2918 1149 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8565
Fragments arranged by size:
8565 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 2841 4719 4924 6471 6820 7614 7634 2256
Size: 585 1878 205 1547 349 794 20 3926
Fragments arranged by size:
3926 1878 1547 794 585 349 205 20
ClaI AT'CG_AT
Cuts at: 1554 4581 1554
Size: 3027 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9269
DraI TTT'AAA
Cuts at: 2361 2928 6608 2361
Size: 567 3680 5057
Fragments arranged by size:
5057 3680 567
DraIII CAC_nnn'GTG
Cuts at: 2471 6398 2471
Size: 3927 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8311 809
Size: 507 6995 1802
Fragments arranged by size:
6995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 6751 7640
Size: 54 1425 50 275 18 1456 3465 889
Cuts at: 7640 9269 8
Size: 1629 43
Fragments arranged by size:
3465 1629 1456 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6751 1537
Size: 275 4939 4090
Fragments arranged by size:
4939 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 2274 3222 3771 4536 5436 7234 1353
Size: 656 265 948 549 765 900 1798 3423
Fragments arranged by size:
3423 1798 948 900 765 656 549 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4683 7891 8265 8411 387
Size: 1064 138 3094 3208 374 146 1280
Fragments arranged by size:
3208 3094 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2812 4272 4347 4500 4658 4913 5178
Size: 393 1180 1460 75 153 158 255 265
Cuts at: 5178 5313 5535 5657 5715 6288 7367 7964 8751
Size: 135 222 122 58 573 1079 597 787
Cuts at: 8751 9019 1239
Size: 268 1524
Fragments arranged by size:
1524 1460 1180 1079 787 597 573 393
268 265 255 222 158 153 135 122
75 58
EcoNI CCTnn'n_nnAGG
Cuts at: 5835 5835
Size: 9304
EcoO109I rG'GnC_Cy
Cuts at: 5048 5593 5048
Size: 545 8759
EcoRI G'AATT_C
Cuts at: 3367 6613 6991 3367
Size: 3246 378 5680
Fragments arranged by size:
5680 3246 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5486 5486
Size: 9304
FspI TGC'GCA
Cuts at: 7810 7810
Size: 9304
HaeII r_GCGC'y
Cuts at: 1678 4926 8451 1678
Size: 3248 3525 2531
Fragments arranged by size:
3525 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3014 3028 3046 3060 3608
Size: 32 135 32 1442 14 18 14 548
Cuts at: 3608 3640 3936 3968 4245 4277 4498 4530 5771
Size: 32 296 32 277 32 221 32 1241
Cuts at: 5771 5803 6021 6053 6566 6598 7945 7977 8019
Size: 32 218 32 513 32 1347 32 42
Cuts at: 8019 8051 1373
Size: 32 2626
Fragments arranged by size:
2626 1442 1347 1241 548 513 296 277
221 218 135 42 32 32 32 32
32 32 32 32 32 32 32 18
14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3733 4987 6648 6894 669
Size: 988 281 1795 1254 1661 246 3079
Fragments arranged by size:
3079 1795 1661 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3347 3377 748
Size: 2599 30 6675
Fragments arranged by size:
6675 2599 30
HpaI GTT'AAC
Cuts at: 4987 6894 4987
Size: 1907 7397
KasI G'GCGC_C
Cuts at: 4922 4922
Size: 9304
KpnI G_GTAC'C
Cuts at: 6629 6629
Size: 9304
MfeI C'AATT_G
Cuts at: 6903 6903
Size: 9304
MluI A'CGCG_T
Cuts at: 9180 9180
Size: 9304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 5324 6168 8418 8602 1305
Size: 799 3220 844 2250 184 2007
Fragments arranged by size:
3220 2250 2007 844 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3288 10
Size: 54 1425 343 1456 6026
Fragments arranged by size:
6026 1456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2679 5877 6023 7262 7621 7780
Size: 648 843 670 3198 146 1239 359 159
Cuts at: 7780 518
Size: 2042
Fragments arranged by size:
3198 2042 1239 843 670 648 359 159
146
MspA1I CmG'CkG
Cuts at: 1606 5333 6005 6266 6720 7397 8545 8790 1606
Size: 3727 672 261 454 677 1148 245 2120
Fragments arranged by size:
3727 2120 1148 677 672 454 261 245
NaeI GCC'GGC
Cuts at: 5103 5584 5103
Size: 481 8823
NarI GG'CG_CC
Cuts at: 4923 4923
Size: 9304
NcoI C'CATG_G
Cuts at: 513 1065 2214 5411 513
Size: 552 1149 3197 4406
Fragments arranged by size:
4406 3197 1149 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9304
NgoMIV G'CCGG_C
Cuts at: 5101 5582 5101
Size: 481 8823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9304
NotI GC'GGCC_GC
Cuts at: 6751 6751
Size: 9304
NspI r_CATG'y
Cuts at: 1132 1229 2383 3811 6444 6662 8207 1132
Size: 97 1154 1428 2633 218 1545 2229
Fragments arranged by size:
2633 2229 1545 1428 1154 218 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9304
PciI A'CATG_T
Cuts at: 6440 8203 6440
Size: 1763 7541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4250 5412 1108
Size: 3142 1162 5000
Fragments arranged by size:
5000 3142 1162
PfoI T'CCnGG_A
Cuts at: 4555 4555
Size: 9304
PmeI GTTT'AAAC
Cuts at: 6608 6608
Size: 9304
PmlI CAC'GTG
Cuts at: 2156 2156
Size: 9304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 7373 7405 8886 8918 5438
Size: 32 1903 32 1481 32 5824
Fragments arranged by size:
5824 1903 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5048 5048
Size: 9304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9304
PsiI TTA'TAA
Cuts at: 6874 6874
Size: 9304
PspXI vC'TCGA_Gb
Cuts at: 6530 6530
Size: 9304
PstI C_TGCA'G
Cuts at: 830 2503 3607 6656 830
Size: 1673 1104 3049 3478
Fragments arranged by size:
3478 3049 1673 1104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9304
SalI G'TCGA_C
Cuts at: 1936 6646 1936
Size: 4710 4594
SapI GCTCTTCn'nnn_
Cuts at: 3222 3771 3222
Size: 549 8755
SbfI CC_TGCA'GG
Cuts at: 6656 6656
Size: 9304
ScaI AGT'ACT
Cuts at: 3459 7552 9264 3459
Size: 4093 1712 3499
Fragments arranged by size:
4093 3499 1712
SexAI A'CCwGG_T
Cuts at: 6394 6394
Size: 9304
SfcI C'TryA_G
Cuts at: 826 1045 2499 3603 5058 6652 7787 8468 8659
Size: 219 1454 1104 1455 1594 1135 681 191
Cuts at: 8659 826
Size: 1471
Fragments arranged by size:
1594 1471 1455 1454 1135 1104 681 219
191
SfoI GGC'GCC
Cuts at: 4924 4924
Size: 9304
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9304
SmaI CCC'GGG
Cuts at: 1872 6631 1872
Size: 4759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2789 5460 6530 7408 8309 8571
Size: 197 926 846 2671 1070 878 901 262
Cuts at: 8571 820
Size: 1553
Fragments arranged by size:
2671 1553 1070 926 901 878 846 262
197
SnaBI TAC'GTA
Cuts at: 493 7095 493
Size: 6602 2702
SpeI A'CTAG_T
Cuts at: 152 2135 2795 152
Size: 1983 660 6661
Fragments arranged by size:
6661 1983 660
SphI G_CATG'C
Cuts at: 1132 2383 3811 6662 1132
Size: 1251 1428 2851 3774
Fragments arranged by size:
3774 2851 1428 1251
SspI AAT'ATT
Cuts at: 5 52 4133 7228 5
Size: 47 4081 3095 2081
Fragments arranged by size:
4081 3095 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9304
StyI C'CwwG_G
Cuts at: 513 1065 2214 3283 5411 5589 6743 513
Size: 552 1149 1069 2128 178 1154 3074
Fragments arranged by size:
3074 2128 1154 1149 1069 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 5012 5957 7331 7348 7501 7686
Size: 1255 902 2530 945 1374 17 153 185
Cuts at: 7686 8131 325
Size: 445 1498
Fragments arranged by size:
2530 1498 1374 1255 945 902 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3439 3457 7550
Size: 274 80 33 51 1443 1462 18 4093
Cuts at: 7550 9262 96
Size: 1712 138
Fragments arranged by size:
4093 1712 1462 1443 274 138 80 51
33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9264 9296 9264
Size: 32 9272
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9304
XbaI T'CTAG_A
Cuts at: 6640 6640
Size: 9304
XhoI C'TCGA_G
Cuts at: 1943 6530 1943
Size: 4587 4717
XmaI C'CCGG_G
Cuts at: 1870 6629 1870
Size: 4759 4545
XmnI GAAnn'nnTTC
Cuts at: 2479 3757 4632 5469 7433 2479
Size: 1278 875 837 1964 4350
Fragments arranged by size:
4350 1964 1278 875 837
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7112 276
Size: 53 83 186 936 5578 2468
Fragments arranged by size:
5578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI
Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI