Restriction map for
pFN21AA0580
(Circular) (Six-base) MAPSORT of: pFN21AA0580.seq Check: 6956 from: 1 to: 9817
pFN21AA0580 9817 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6187 6187
Size: 9817
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7627 278
Size: 53 83 186 936 6091 2468
Fragments arranged by size:
6091 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4820 7160 1937
Size: 2883 2340 4594
Fragments arranged by size:
4594 2883 2340
Acc65I G'GTAC_C
Cuts at: 7138 7138
Size: 9817
AclI AA'CG_TT
Cuts at: 1563 6655 7944 8317 1563
Size: 5092 1289 373 3063
Fragments arranged by size:
5092 3063 1289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4719 6948 7880 9264 9532 1632
Size: 3087 2229 932 1384 268 1917
Fragments arranged by size:
3087 2229 1917 1384 932 268
AflII C'TTAA_G
Cuts at: 820 1017 5182 820
Size: 197 4165 5455
Fragments arranged by size:
5455 4165 197
AflIII A'CryG_T
Cuts at: 1203 4939 8716 9693 1203
Size: 3736 3777 977 1327
Fragments arranged by size:
3777 3736 1327 977
AgeI A'CCGG_T
Cuts at: 6378 8648 6378
Size: 2270 7547
AhdI GACnn_n'nnGTC
Cuts at: 8546 8546
Size: 9817
AleI CACnn'nnGTG
Cuts at: 1166 6285 1166
Size: 5119 4698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9817
AlwNI CAG_nnn'CTG
Cuts at: 2645 3107 4101 4127 5042 6926 7560 9132 2645
Size: 462 994 26 915 1884 634 1572 3330
Fragments arranged by size:
3330 1884 1572 994 915 634 462 26
ApaI G_GGCC'C
Cuts at: 4905 4905
Size: 9817
ApaLI G'TGCA_C
Cuts at: 7874 9030 7874
Size: 1156 8661
ApoI r'AATT_y
Cuts at: 1493 2488 2686 2968 2983 3037 3065 3289 3521
Size: 995 198 282 15 54 28 224 232
Cuts at: 3521 3629 3810 4397 4595 4613 7126 7352 7504
Size: 108 181 587 198 18 2513 226 152
Cuts at: 7504 9613 1493
Size: 2109 1697
Fragments arranged by size:
2513 2109 1697 995 587 282 232 226
224 198 198 181 152 108 54 28
18 15
AseI AT'TA_AT
Cuts at: 160 1032 2058 4842 5874 8371 160
Size: 872 1026 2784 1032 2497 1606
Fragments arranged by size:
2784 2497 1606 1032 1026 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9817
AvaI C'yCGr_G
Cuts at: 1870 1943 3198 7142 1870
Size: 73 1255 3944 4545
Fragments arranged by size:
4545 3944 1255 73
BamHI G'GATC_C
Cuts at: 7147 7514 7147
Size: 367 9450
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4835 6549 7138 8593 618
Size: 325 217 627 3048 1714 589 1455 1842
Fragments arranged by size:
3048 1842 1714 1455 627 589 325 217
BanII G_rGCy'C
Cuts at: 1391 4905 5619 7136 1391
Size: 3514 714 1517 4072
Fragments arranged by size:
4072 3514 1517 714
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 2564 4662 5194 6986 928
Size: 971 495 170 2098 532 1792 3759
Fragments arranged by size:
3759 2098 1792 971 532 495 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6185 6219 8008 8042 1903
Size: 34 4248 34 1789 34 3678
Fragments arranged by size:
4248 3678 1789 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2175 2430 3186 7709 8919 2175
Size: 255 756 4523 1210 3073
Fragments arranged by size:
4523 3073 1210 756 255
BclI T'GATC_A
Cuts at: 1548 2299 2607 7018 1548
Size: 751 308 4411 4347
Fragments arranged by size:
4411 4347 751 308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6960 8428 136
Size: 107 122 71 631 605 5288 1468 1525
Fragments arranged by size:
5288 1525 1468 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4187 4920 9812 4187
Size: 733 4892 4192
Fragments arranged by size:
4892 4192 733
BlpI GC'TnA_GC
Cuts at: 5372 7234 5372
Size: 1862 7955
Bme1580I G_kGCm'C
Cuts at: 1790 4905 7878 9034 1790
Size: 3115 2973 1156 2573
Fragments arranged by size:
3115 2973 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9817
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4141 4669 4908
Size: 416 53 83 399 24 2724 528 239
Cuts at: 4908 6311 7548 8506 442
Size: 1403 1237 958 1753
Fragments arranged by size:
2724 1753 1403 1237 958 528 416 399
239 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4239 4565 1056
Size: 3183 326 6308
Fragments arranged by size:
6308 3183 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3104
Size: 9817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5513 6000 6212 6872 6932 8477 1239
Size: 4274 487 212 660 60 1545 2579
Fragments arranged by size:
4274 2579 1545 660 487 212 60
Bpu10I CC'TnA_GC
Cuts at: 3533 3533
Size: 9817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3471 3735 4816 4953 7942 8807 9105 3471
Size: 264 1081 137 2989 865 298 4183
Fragments arranged by size:
4183 2989 1081 865 298 264 137
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 4527 8480 882
Size: 617 1506 1522 3953 2219
Fragments arranged by size:
3953 2219 1522 1506 617
BsaAI yAC'GTr
Cuts at: 493 1204 7608 493
Size: 711 6404 2702
Fragments arranged by size:
6404 2702 711
BsaBI GATnn'nnATC
Cuts at: 9811 9811
Size: 9817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7624 8006 8641 275
Size: 53 83 186 936 6091 382 635 1451
Fragments arranged by size:
6091 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4471 4529 6291 6378 8250 8648 8922 9069 9480
Size: 58 1762 87 1872 398 274 147 411
Cuts at: 9480 4471
Size: 4808
Fragments arranged by size:
4808 1872 1762 411 398 274 147 87
58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4500 4524 4530 4554 4916
Size: 30 165 30 3097 24 6 24 362
Cuts at: 4916 4946 1178
Size: 30 6049
Fragments arranged by size:
6049 3097 362 165 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2436 2993 3474 3698 1186
Size: 1250 557 481 224 7305
Fragments arranged by size:
7305 1250 557 481 224
BseYI C'CCAG_C
Cuts at: 4574 6513 6609 9020 9516 4574
Size: 1939 96 2411 496 4875
Fragments arranged by size:
4875 2411 1939 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 4046 4125 4194 1163
Size: 573 403 1907 79 69 6786
Fragments arranged by size:
6786 1907 573 403 79 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7267 8028 9056 1540
Size: 275 186 5266 761 1028 2301
Fragments arranged by size:
5266 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5619 6858 7878 7963 9034 5619
Size: 1239 1020 85 1071 6402
Fragments arranged by size:
6402 1239 1071 1020 85
BsmI GAATG_Cn'
Cuts at: 2654 3269 5181 5788 6724 7328 7421 2654
Size: 615 1912 607 936 604 93 5050
Fragments arranged by size:
5050 1912 936 615 607 604 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4905 5619 6858 7136 7878 7963 9034
Size: 399 3115 714 1239 278 742 85 1071
Cuts at: 9034 1391
Size: 2174
Fragments arranged by size:
3115 2174 1239 1071 742 714 399 278
85
BspEI T'CCGG_A
Cuts at: 9480 9480
Size: 9817
BspHI T'CATG_A
Cuts at: 7706 9436 7706
Size: 1730 8087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5327 6187 7172 844
Size: 4483 860 985 3489
Fragments arranged by size:
4483 3489 985 860
BsrBI CCG'CTC
Cuts at: 1394 1823 7704 1394
Size: 429 5881 3507
Fragments arranged by size:
5881 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4255 8312 8486 65
Size: 1183 3007 4057 174 1396
Fragments arranged by size:
4057 3007 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6378 8461 8648 1700
Size: 110 4568 2083 187 2869
Fragments arranged by size:
4568 2869 2083 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9817
BssSI C'ACGA_G
Cuts at: 7877 8889 7877
Size: 1012 8805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3107 4585 794
Size: 2313 1478 6026
Fragments arranged by size:
6026 2313 1478
BstBI TT'CG_AA
Cuts at: 3572 6874 8621 3572
Size: 3302 1747 4768
Fragments arranged by size:
4768 3302 1747
BstEII G'GTnAC_C
Cuts at: 1184 2168 1184
Size: 984 8833
BstXI CCAn_nnnn'nTGG
Cuts at: 2093 6513 2093
Size: 4420 5397
BstYI r'GATC_y
Cuts at: 1972 4123 4187 4920 5631 7147 7514 7899 7916
Size: 2151 64 733 711 1516 367 385 17
Cuts at: 7916 9368 9454 9466 9812 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2151 1977 1516 1452 733 711 385 367
346 86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 5281 6804 6834 513
Size: 552 4216 1523 30 3496
Fragments arranged by size:
4216 3496 1523 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9078
Fragments arranged by size:
9078 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 3228 5600 6280 6292 6844 7333 8127 8147
Size: 762 2372 680 12 552 489 794 20
Cuts at: 8147 2466
Size: 4136
Fragments arranged by size:
4136 2372 794 762 680 552 489 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2343 2378 529
Size: 35 1779 35 7968
Fragments arranged by size:
7968 1779 35 35
DraI TTT'AAA
Cuts at: 4893 5524 7121 4893
Size: 631 1597 7589
Fragments arranged by size:
7589 1597 631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8824 809
Size: 507 7508 1802
Fragments arranged by size:
7508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6629 7264 8153
Size: 54 1425 50 275 18 4799 635 889
Cuts at: 8153 9782 8
Size: 1629 43
Fragments arranged by size:
4799 1629 1425 889 635 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7264 1537
Size: 275 5452 4090
Fragments arranged by size:
5452 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3026 3675 4738 4749 5072 5703 6147 6174
Size: 1673 649 1063 11 323 631 444 27
Cuts at: 6174 6245 6422 7747 1353
Size: 71 177 1325 3423
Fragments arranged by size:
3423 1673 1325 1063 649 631 444 323
177 71 27 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8404 8778 8924 387
Size: 1064 138 6815 374 146 1280
Fragments arranged by size:
6815 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5617 5617
Size: 9817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4719 5513 6000 6212 6872 6932 6948
Size: 393 3087 794 487 212 660 60 16
Cuts at: 6948 7880 8477 9264 9532 1239
Size: 932 597 787 268 1524
Fragments arranged by size:
3087 1524 932 794 787 660 597 487
393 268 212 60 16
EcoNI CCTnn'n_nnAGG
Cuts at: 6111 6111
Size: 9817
EcoO109I rG'GnC_Cy
Cuts at: 5866 5866
Size: 9817
EcoRI G'AATT_C
Cuts at: 2983 7126 7504 2983
Size: 4143 378 5296
Fragments arranged by size:
5296 4143 378
EcoRV GAT'ATC
Cuts at: 3782 6045 3782
Size: 2263 7554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6274 6953 6274
Size: 679 9138
FspI TGC'GCA
Cuts at: 8323 8323
Size: 9817
HaeII r_GCGC'y
Cuts at: 1678 8964 1678
Size: 7286 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 2551 2583 2972
Size: 32 135 32 854 32 93 32 389
Cuts at: 2972 3004 3464 3496 4121 4153 4500 4532 6439
Size: 32 460 32 625 32 347 32 1907
Cuts at: 6439 6471 6785 6817 8458 8490 8532 8564 1373
Size: 32 314 32 1641 32 42 32 2626
Fragments arranged by size:
2626 1907 1641 854 625 460 389 347
314 135 93 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 5063 5710 7161 7407 669
Size: 988 281 951 2174 647 1451 246 3079
Fragments arranged by size:
3079 2174 1451 988 951 647 281 246
HindIII A'AGCT_T
Cuts at: 748 5518 748
Size: 4770 5047
HpaI GTT'AAC
Cuts at: 7407 7407
Size: 9817
KpnI G_GTAC'C
Cuts at: 7142 7142
Size: 9817
MfeI C'AATT_G
Cuts at: 5322 7416 5322
Size: 2094 7723
MluI A'CGCG_T
Cuts at: 9693 9693
Size: 9817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 4116 5277 6530 8931 9115 1305
Size: 1038 1773 1161 1253 2401 184 2007
Fragments arranged by size:
2401 2007 1773 1253 1161 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6631 10
Size: 54 1425 343 4799 3196
Fragments arranged by size:
4799 3196 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 5396 5481 5781 6285 6618 7775
Size: 648 919 3311 85 300 504 333 1157
Cuts at: 7775 8134 8293 518
Size: 359 159 2042
Fragments arranged by size:
3311 2042 1157 919 648 504 359 333
300 159 85
MspA1I CmG'CkG
Cuts at: 1606 5406 6266 6638 7233 7910 9058 9303 1606
Size: 3800 860 372 595 677 1148 245 2120
Fragments arranged by size:
3800 2120 1148 860 677 595 372 245
NcoI C'CATG_G
Cuts at: 513 1065 5281 6834 513
Size: 552 4216 1553 3496
Fragments arranged by size:
4216 3496 1553 552
NdeI CA'TA_TG
Cuts at: 387 4338 387
Size: 3951 5866
NheI G'CTAG_C
Cuts at: 1052 4235 4561 1052
Size: 3183 326 6308
Fragments arranged by size:
6308 3183 326
NotI GC'GGCC_GC
Cuts at: 7264 7264
Size: 9817
NspI r_CATG'y
Cuts at: 1132 1229 3502 7175 8720 1132
Size: 97 2273 3673 1545 2229
Fragments arranged by size:
3673 2273 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 8089
PciI A'CATG_T
Cuts at: 8716 8716
Size: 9817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5089 5794 6638 6850 1108
Size: 3981 705 844 212 4075
Fragments arranged by size:
4075 3981 844 705 212
PfoI T'CCnGG_A
Cuts at: 5490 5490
Size: 9817
PmeI GTTT'AAAC
Cuts at: 7121 7121
Size: 9817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7886 7918 9399 9431 7886
Size: 32 1481 32 8272
Fragments arranged by size:
8272 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5866 5866
Size: 9817
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9817
PsiI TTA'TAA
Cuts at: 6035 7387 6035
Size: 1352 8465
PspOMI G'GGCC_C
Cuts at: 4901 4901
Size: 9817
PstI C_TGCA'G
Cuts at: 830 2145 2473 6287 7169 830
Size: 1315 328 3814 882 3478
Fragments arranged by size:
3814 3478 1315 882 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9817
PvuII CAG'CTG
Cuts at: 5406 6638 5406
Size: 1232 8585
SacI G_AGCT'C
Cuts at: 5619 5619
Size: 9817
SalI G'TCGA_C
Cuts at: 1936 7159 1936
Size: 5223 4594
SapI GCTCTTCn'nnn_
Cuts at: 6147 6147
Size: 9817
SbfI CC_TGCA'GG
Cuts at: 7169 7169
Size: 9817
ScaI AGT'ACT
Cuts at: 8065 9777 8065
Size: 1712 8105
SexAI A'CCwGG_T
Cuts at: 6240 6240
Size: 9817
SfcI C'TryA_G
Cuts at: 826 1045 2141 2163 2469 3964 4461 6025 6283
Size: 219 1096 22 306 1495 497 1564 258
Cuts at: 6283 7165 8300 8981 9172 826
Size: 882 1135 681 191 1471
Fragments arranged by size:
1564 1495 1471 1135 1096 882 681 497
306 258 219 191 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 6960 6960
Size: 9817
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9817
SmaI CCC'GGG
Cuts at: 1872 7144 1872
Size: 5272 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3198 3486 3750 4795 4968 5182
Size: 197 926 1255 288 264 1045 173 214
Cuts at: 5182 7921 8822 9084 820
Size: 2739 901 262 1553
Fragments arranged by size:
2739 1553 1255 1045 926 901 288 264
262 214 197 173
SnaBI TAC'GTA
Cuts at: 493 7608 493
Size: 7115 2702
SpeI A'CTAG_T
Cuts at: 152 5840 152
Size: 5688 4129
SphI G_CATG'C
Cuts at: 1132 7175 1132
Size: 6043 3774
SspI AAT'ATT
Cuts at: 5 52 2530 5892 6100 6742 7741 5
Size: 47 2478 3362 208 642 999 2081
Fragments arranged by size:
3362 2478 2081 999 642 208 47
StuI AGG'CCT
Cuts at: 1517 6963 1517
Size: 5446 4371
StyI C'CwwG_G
Cuts at: 513 1065 4209 5281 5799 6726 6834 7256 513
Size: 552 3144 1072 518 927 108 422 3074
Fragments arranged by size:
3144 3074 1072 927 552 518 422 108
SwaI ATTT'AAAT
Cuts at: 4893 4893
Size: 9817
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3505 3942 5978 7844 7861 8014 8199
Size: 1255 1925 437 2036 1866 17 153 185
Cuts at: 8199 8644 325
Size: 445 1498
Fragments arranged by size:
2036 1925 1866 1498 1255 445 437 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6187 6296 8063
Size: 274 80 33 51 1443 4210 109 1767
Cuts at: 8063 9775 96
Size: 1712 138
Fragments arranged by size:
4210 1767 1712 1443 274 138 109 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3822 3854 4524 4556 9777 9809 3822
Size: 32 670 32 5221 32 3830
Fragments arranged by size:
5221 3830 670 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9817
XbaI T'CTAG_A
Cuts at: 4480 7153 4480
Size: 2673 7144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4128 4128
Size: 9817
XhoI C'TCGA_G
Cuts at: 1943 3198 1943
Size: 1255 8562
XmaI C'CCGG_G
Cuts at: 1870 7142 1870
Size: 5272 4545
XmnI GAAnn'nnTTC
Cuts at: 2686 3267 3289 6254 6655 7946 2686
Size: 581 22 2965 401 1291 4557
Fragments arranged by size:
4557 2965 1291 581 401 22
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7625 276
Size: 53 83 186 936 6091 2468
Fragments arranged by size:
6091 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SfoI SrfI