Restriction map for
pFN21AA0579
(Circular) (Six-base) MAPSORT of: pFN21AA0579.seq Check: 3273 from: 1 to: 7552
pFN21AA0579 7552 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2035 2857 2035
Size: 822 6730
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5362 278
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4895 1937
Size: 2958 4594
Acc65I G'GTAC_C
Cuts at: 2544 2669 4873 2544
Size: 125 2204 5223
Fragments arranged by size:
5223 2204 125
AclI AA'CG_TT
Cuts at: 1563 5679 6052 1563
Size: 4116 373 3063
Fragments arranged by size:
4116 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2011 2330 2430 2472 2612 3012 3842 4468
Size: 379 319 100 42 140 400 830 626
Cuts at: 4468 5615 6999 7267 1632
Size: 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 830 626 400 379 319
268 140 100 42
AfeI AGC'GCT
Cuts at: 3319 3319
Size: 7552
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7355
AflIII A'CryG_T
Cuts at: 1203 2568 3337 6451 7428 1203
Size: 1365 769 3114 977 1327
Fragments arranged by size:
3114 1365 1327 977 769
AgeI A'CCGG_T
Cuts at: 6383 6383
Size: 7552
AhdI GACnn_n'nnGTC
Cuts at: 6281 6281
Size: 7552
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7552
AlwNI CAG_nnn'CTG
Cuts at: 2050 3496 4589 5295 6867 2050
Size: 1446 1093 706 1572 2735
Fragments arranged by size:
2735 1572 1446 1093 706
ApaI G_GGCC'C
Cuts at: 3165 4088 3165
Size: 923 6629
ApaLI G'TGCA_C
Cuts at: 3542 3593 4366 4553 5609 6765 3542
Size: 51 773 187 1056 1156 4329
Fragments arranged by size:
4329 1156 1056 773 187 51
ApoI r'AATT_y
Cuts at: 1493 2420 3008 3535 4861 5087 5239 7348 1493
Size: 927 588 527 1326 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 927 588 527 226 152
AscI GG'CGCG_CC
Cuts at: 2115 2115
Size: 7552
AseI AT'TA_AT
Cuts at: 160 1032 6106 160
Size: 872 5074 1606
Fragments arranged by size:
5074 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3135 3237 3297 4399 4877 1870
Size: 73 177 1015 102 60 1102 478 4545
Fragments arranged by size:
4545 1102 1015 478 177 102 73 60
BamHI G'GATC_C
Cuts at: 4882 5249 4882
Size: 367 7185
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2544 2669 3087 4324 4873
Size: 325 217 627 757 125 418 1237 549
Cuts at: 4873 6328 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1237 757 627 549 418 325
217 125
BanII G_rGCy'C
Cuts at: 1391 2064 2740 2813 3165 3311 3362 4088 4871
Size: 673 676 73 352 146 51 726 783
Cuts at: 4871 1391
Size: 4072
Fragments arranged by size:
4072 783 726 676 673 352 146 73
51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3579 3830 928
Size: 971 1680 251 4650
Fragments arranged by size:
4650 1680 971 251
BbvCI CC'TCA_GC
Cuts at: 3321 3321
Size: 7552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5743 5777 1903
Size: 34 3806 34 3678
Fragments arranged by size:
3806 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2726 5444 6654 2726
Size: 2718 1210 3624
Fragments arranged by size:
3624 2718 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7552
BfrBI ATG'CAT
Cuts at: 3800 3800
Size: 7552
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 2106 2263
Size: 107 122 71 631 605 416 18 157
Cuts at: 2263 4163 6163 136
Size: 1900 2000 1525
Fragments arranged by size:
2000 1900 1525 631 605 416 157 122
107 71 18
BglII A'GATC_T
Cuts at: 2998 7547 2998
Size: 4549 3003
BlpI GC'TnA_GC
Cuts at: 2482 2812 3497 4969 2482
Size: 330 685 1472 5065
Fragments arranged by size:
5065 1472 685 330
Bme1580I G_kGCm'C
Cuts at: 1790 3144 3165 3546 3597 4002 4088 4370 4557
Size: 1354 21 381 51 405 86 282 187
Cuts at: 4557 5613 6769 1790
Size: 1056 1156 2573
Fragments arranged by size:
2573 1354 1156 1056 405 381 282 187
86 51 21
BmgBI CAC'GTC
Cuts at: 1318 3157 3338 3688 4149 4371 1318
Size: 1839 181 350 461 222 4499
Fragments arranged by size:
4499 1839 461 350 222 181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5283 6241 442
Size: 416 53 83 399 24 3866 958 1753
Fragments arranged by size:
3866 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7552
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 3766 3472
Size: 294 7258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3077 3247 3259 4180 4809 6212 1239
Size: 1838 170 12 921 629 1403 2579
Fragments arranged by size:
2579 1838 1403 921 629 170 12
Bpu10I CC'TnA_GC
Cuts at: 2918 3321 2918
Size: 403 7149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 5677 6542 6840 3029
Size: 2648 865 298 3741
Fragments arranged by size:
3741 2648 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3848 6215 882
Size: 617 2349 2367 2219
Fragments arranged by size:
2367 2349 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 2569 5343 493
Size: 711 1317 48 2774 2702
Fragments arranged by size:
2774 2702 1317 711 48
BsaBI GATnn'nnATC
Cuts at: 7546 7546
Size: 7552
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3878 5359 5741 6376
Size: 53 83 186 936 2345 1481 382 635
Cuts at: 6376 275
Size: 1451
Fragments arranged by size:
2345 1481 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2491 2826 4448 5985 6383 6657 6804 7215 2491
Size: 335 1622 1537 398 274 147 411 2828
Fragments arranged by size:
2828 1622 1537 411 398 335 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2596 2626 3251 3281 4111
Size: 30 165 30 1193 30 625 30 830
Cuts at: 4111 4141 4430 4460 1178
Size: 30 289 30 4270
Fragments arranged by size:
4270 1193 830 625 289 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 3259 3262 3338 3569 1186
Size: 1075 998 3 76 231 5169
Fragments arranged by size:
5169 1075 998 231 76 3
BseYI C'CCAG_C
Cuts at: 2149 2739 3051 3881 4130 4175 4183 4190 4518
Size: 590 312 830 249 45 8 7 328
Cuts at: 4518 6755 7251 2149
Size: 2237 496 2450
Fragments arranged by size:
2450 2237 830 590 496 328 312 249
45 8 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 3188 3461 3740 4516 1163
Size: 573 471 981 273 279 776 4199
Fragments arranged by size:
4199 981 776 573 471 279 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2763 3867 5002 5763 6791 1540
Size: 275 186 762 1104 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1104 1028 762 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3311 3362 3546 3597 4370 4557 5613 5698
Size: 1247 51 184 51 773 187 1056 85
Cuts at: 5698 6769 2064
Size: 1071 2847
Fragments arranged by size:
2847 1247 1071 1056 773 187 184 85
51 51
BsmI GAATG_Cn'
Cuts at: 2221 5063 5156 2221
Size: 2842 93 4617
Fragments arranged by size:
4617 2842 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3076 3076
Size: 7552
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2740 2813 3144 3165 3311 3362
Size: 399 274 676 73 331 21 146 51
Cuts at: 3362 3546 3597 4002 4088 4370 4557 4871 5613
Size: 184 51 405 86 282 187 314 742
Cuts at: 5613 5698 6769 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 676 405 399 331 314
282 274 187 184 146 86 85 73
51 51 21
BspEI T'CCGG_A
Cuts at: 2491 7215 2491
Size: 4724 2828
BspHI T'CATG_A
Cuts at: 5441 7171 5441
Size: 1730 5822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 2857 3712 4907 844
Size: 1191 822 855 1195 3489
Fragments arranged by size:
3489 1195 1191 855 822
BsrBI CCG'CTC
Cuts at: 1394 1823 2733 4713 5439 1394
Size: 429 910 1980 726 3507
Fragments arranged by size:
3507 1980 910 726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4071 4696 6047 6221 65
Size: 1183 2823 625 1351 174 1396
Fragments arranged by size:
2823 1396 1351 1183 625 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2762 3376 3838 4791 6196 6383 1700
Size: 110 952 614 462 953 1405 187 2869
Fragments arranged by size:
2869 1405 953 952 614 462 187 110
BsrGI T'GTAC_A
Cuts at: 96 4032 96
Size: 3936 3616
BssHII G'CGCG_C
Cuts at: 1925 2115 2133 1925
Size: 190 18 7344
Fragments arranged by size:
7344 190 18
BssSI C'ACGA_G
Cuts at: 5612 6624 5612
Size: 1012 6540
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 4365 794
Size: 1256 2315 3981
Fragments arranged by size:
3981 2315 1256
BstBI TT'CG_AA
Cuts at: 6356 6356
Size: 7552
BstEII G'GTnAC_C
Cuts at: 1184 3115 4748 1184
Size: 1931 1633 3988
Fragments arranged by size:
3988 1931 1633
BstXI CCAn_nnnn'nTGG
Cuts at: 3283 3283
Size: 7552
BstYI r'GATC_y
Cuts at: 1972 2070 2524 2998 3629 4882 5249 5634 5651
Size: 98 454 474 631 1253 367 385 17
Cuts at: 5651 7103 7189 7201 7547 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1253 631 474 454 385 367
346 98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2269 2540 2269
Size: 271 7281
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 2241 2746 2967 3024 3282
Size: 552 939 97 140 505 221 57 258
Cuts at: 3282 3420 3870 4779 513
Size: 138 450 909 3286
Fragments arranged by size:
3286 939 909 552 505 450 258 221
140 138 97 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6813
Fragments arranged by size:
6813 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2785 3130 4119 4249 5068 5862 5882 2200
Size: 585 345 989 130 819 794 20 3870
Fragments arranged by size:
3870 989 819 794 585 345 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4135 4170 529
Size: 35 3571 35 3911
Fragments arranged by size:
3911 3571 35 35
DraI TTT'AAA
Cuts at: 4856 4856
Size: 7552
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6559 809
Size: 507 5243 1802
Fragments arranged by size:
5243 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 3864 4164
Size: 54 1425 50 275 18 930 1104 300
Cuts at: 4164 4793 4999 5888 7517 8
Size: 629 206 889 1629 43
Fragments arranged by size:
1629 1425 1104 930 889 629 300 275
206 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2760 3864 4999 1537
Size: 275 948 1104 1135 4090
Fragments arranged by size:
4090 1135 1104 948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 3297 5482 1353
Size: 1095 849 2185 3423
Fragments arranged by size:
3423 2185 1095 849
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2758 3400 3897 6139 6513 6659
Size: 1064 138 1169 642 497 2242 374 146
Cuts at: 6659 387
Size: 1280
Fragments arranged by size:
2242 1280 1169 1064 642 497 374 146
138
EcoICRI GAG'CTC
Cuts at: 2062 3309 3360 2062
Size: 1247 51 6254
Fragments arranged by size:
6254 1247 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2330 2430 2472 2612 3012 3077
Size: 393 379 319 100 42 140 400 65
Cuts at: 3077 3247 3259 3842 4180 4468 4809 5615 6212
Size: 170 12 583 338 288 341 806 597
Cuts at: 6212 6999 7267 1239
Size: 787 268 1524
Fragments arranged by size:
1524 806 787 597 583 400 393 379
341 338 319 288 268 170 140 100
65 42 12
EcoO109I rG'GnC_Cy
Cuts at: 2182 2265 3724 4085 4578 2182
Size: 83 1459 361 493 5156
Fragments arranged by size:
5156 1459 493 361 83
EcoRI G'AATT_C
Cuts at: 3535 4861 5239 3535
Size: 1326 378 5848
Fragments arranged by size:
5848 1326 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 7552
FspI TGC'GCA
Cuts at: 6058 6058
Size: 7552
HaeII r_GCGC'y
Cuts at: 1678 3321 3678 4208 4518 6699 1678
Size: 1643 357 530 310 2181 2531
Fragments arranged by size:
2531 2181 1643 530 357 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 2623 2655 6193
Size: 32 135 32 944 32 75 32 3538
Cuts at: 6193 6225 6267 6299 1373
Size: 32 42 32 2626
Fragments arranged by size:
3538 2626 944 135 75 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4896 5142 669
Size: 988 281 2958 246 3079
Fragments arranged by size:
3079 2958 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7552
HpaI GTT'AAC
Cuts at: 5142 5142
Size: 7552
KpnI G_GTAC'C
Cuts at: 2548 2673 4877 2548
Size: 125 2204 5223
Fragments arranged by size:
5223 2204 125
MfeI C'AATT_G
Cuts at: 5151 5151
Size: 7552
MluI A'CGCG_T
Cuts at: 7428 7428
Size: 7552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 3336 6666 6850 1305
Size: 732 1299 3330 184 2007
Fragments arranged by size:
3330 2007 1299 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4166 10
Size: 54 1425 343 2334 3396
Fragments arranged by size:
3396 2334 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 3527 3653 5510 5869 6028 518
Size: 648 1692 669 126 1857 359 159 2042
Fragments arranged by size:
2042 1857 1692 669 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 3055 3112 3232 4179 4386 4392 4503 4575
Size: 1449 57 120 947 207 6 111 72
Cuts at: 4575 4968 5645 6793 7038 1606
Size: 393 677 1148 245 2120
Fragments arranged by size:
2120 1449 1148 947 677 393 245 207
120 111 72 57 6
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 7552
NcoI C'CATG_G
Cuts at: 513 1065 2004 2967 3024 3282 4779 513
Size: 552 939 963 57 258 1497 3286
Fragments arranged by size:
3286 1497 963 939 552 258 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7552
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 7552
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7552
NotI GC'GGCC_GC
Cuts at: 4999 4999
Size: 7552
NsiI A_TGCA'T
Cuts at: 3802 3802
Size: 7552
NspI r_CATG'y
Cuts at: 1132 1229 3532 3800 4910 6455 1132
Size: 97 2303 268 1110 1545 2229
Fragments arranged by size:
2303 2229 1545 1110 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7552
PciI A'CATG_T
Cuts at: 6451 6451
Size: 7552
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2642 4675 1108
Size: 1534 2033 3985
Fragments arranged by size:
3985 2033 1534
PmeI GTTT'AAAC
Cuts at: 4856 4856
Size: 7552
PmlI CAC'GTG
Cuts at: 2521 2569 2521
Size: 48 7504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5621 5653 7134 7166 5621
Size: 32 1481 32 6007
Fragments arranged by size:
6007 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3724 4578 3724
Size: 854 6698
PshAI GACnn'nnGTC
Cuts at: 1613 3788 1613
Size: 2175 5377
PsiI TTA'TAA
Cuts at: 5122 5122
Size: 7552
PspOMI G'GGCC_C
Cuts at: 3161 4084 3161
Size: 923 6629
PspXI vC'TCGA_Gb
Cuts at: 3135 4399 3135
Size: 1264 6288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4678 4710 4678
Size: 32 7520
PstI C_TGCA'G
Cuts at: 830 2877 4095 4385 4904 830
Size: 2047 1218 290 519 3478
Fragments arranged by size:
3478 2047 1218 519 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7552
PvuII CAG'CTG
Cuts at: 3055 3232 4179 4392 3055
Size: 177 947 213 6215
Fragments arranged by size:
6215 947 213 177
RsrII CG'GwC_CG
Cuts at: 4451 4451
Size: 7552
SacI G_AGCT'C
Cuts at: 2064 3311 3362 2064
Size: 1247 51 6254
Fragments arranged by size:
6254 1247 51
SalI G'TCGA_C
Cuts at: 1936 4894 1936
Size: 2958 4594
SanDI GG'GwC_CC
Cuts at: 4578 4578
Size: 7552
SbfI CC_TGCA'GG
Cuts at: 4095 4904 4095
Size: 809 6743
ScaI AGT'ACT
Cuts at: 3260 5800 7512 3260
Size: 2540 1712 3300
Fragments arranged by size:
3300 2540 1712
SfcI C'TryA_G
Cuts at: 826 1045 2873 4091 4234 4381 4900 6035 6716
Size: 219 1828 1218 143 147 519 1135 681
Cuts at: 6716 6907 826
Size: 191 1471
Fragments arranged by size:
1828 1471 1218 1135 681 519 219 191
147 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7552
SmaI CCC'GGG
Cuts at: 1872 2122 3239 3299 4879 1872
Size: 250 1117 60 1580 4545
Fragments arranged by size:
4545 1580 1117 250 60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3044 3135 4399 5656 6557 6819
Size: 197 926 1101 91 1264 1257 901 262
Cuts at: 6819 820
Size: 1553
Fragments arranged by size:
1553 1264 1257 1101 926 901 262 197
91
SnaBI TAC'GTA
Cuts at: 493 5343 493
Size: 4850 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7552
SphI G_CATG'C
Cuts at: 1132 3532 3800 4910 1132
Size: 2400 268 1110 3774
Fragments arranged by size:
3774 2400 1110 268
SrfI GCCC'GGGC
Cuts at: 2122 2122
Size: 7552
SspI AAT'ATT
Cuts at: 5 52 5476 5
Size: 47 5424 2081
Fragments arranged by size:
5424 2081 47
StuI AGG'CCT
Cuts at: 1517 2538 2695 1517
Size: 1021 157 6374
Fragments arranged by size:
6374 1021 157
StyI C'CwwG_G
Cuts at: 513 1065 2004 2967 3024 3282 4669 4779 4991
Size: 552 939 963 57 258 1387 110 212
Cuts at: 4991 513
Size: 3074
Fragments arranged by size:
3074 1387 963 939 552 258 212 110
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3535 3775 4639 5579 5596 5749 5934
Size: 1255 1955 240 864 940 17 153 185
Cuts at: 5934 6379 325
Size: 445 1498
Fragments arranged by size:
1955 1498 1255 940 864 445 240 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3258 4032 5798
Size: 274 80 33 51 1443 1281 774 1766
Cuts at: 5798 7510 96
Size: 1712 138
Fragments arranged by size:
1766 1712 1443 1281 774 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2596 2628 2682 2714 2838 2870 3386 3418 4111
Size: 32 54 32 124 32 516 32 693
Cuts at: 4111 4143 4430 4462 7512 7544 2596
Size: 32 287 32 3050 32 2604
Fragments arranged by size:
3050 2604 693 516 287 124 54 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2585 4589 1316
Size: 1269 2004 4279
Fragments arranged by size:
4279 2004 1269
XbaI T'CTAG_A
Cuts at: 2908 3610 4888 2908
Size: 702 1278 5572
Fragments arranged by size:
5572 1278 702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 3127 2897
Size: 230 7322
XhoI C'TCGA_G
Cuts at: 1943 3135 4399 1943
Size: 1192 1264 5096
Fragments arranged by size:
5096 1264 1192
XmaI C'CCGG_G
Cuts at: 1870 2120 3237 3297 4877 1870
Size: 250 1117 60 1580 4545
Fragments arranged by size:
4545 1580 1117 250 60
XmnI GAAnn'nnTTC
Cuts at: 3395 5681 3395
Size: 2286 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5360 276
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
SacII SapI SexAI SfiI SfoI SwaI