Restriction map for
pFN21AA0571
(Circular) (Six-base) MAPSORT of: pFN21AA0571.seq Check: 3153 from: 1 to: 6619
pFN21AA0571 6619 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3284 3284
Size: 6619
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 3315 3962 1937
Size: 886 492 647 4594
Fragments arranged by size:
4594 886 647 492
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3255 4682 6066 6334 1632
Size: 1623 1427 1384 268 1917
Fragments arranged by size:
1917 1623 1427 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 5518 6495 1203
Size: 4315 977 1327
Fragments arranged by size:
4315 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 2600 5348 2600
Size: 2748 3871
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2115 1184
Size: 931 5688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 2033
Size: 32 6587
AlwNI CAG_nnn'CTG
Cuts at: 3665 3850 4362 5934 3665
Size: 185 512 1572 4350
Fragments arranged by size:
4350 1572 512 185
ApaI G_GGCC'C
Cuts at: 3340 3340
Size: 6619
ApaLI G'TGCA_C
Cuts at: 3383 4676 5832 3383
Size: 1293 1156 4170
Fragments arranged by size:
4170 1293 1156
ApoI r'AATT_y
Cuts at: 1493 2669 3928 4154 4306 6415 1493
Size: 1176 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 1176 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 3944 1870
Size: 73 2001 4545
Fragments arranged by size:
4545 2001 73
AvrII C'CTAG_G
Cuts at: 2870 2870
Size: 6619
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 3908 3940 5395 618
Size: 325 217 627 744 1377 32 1455 1842
Fragments arranged by size:
1842 1455 1377 744 627 325 217 32
BanII G_rGCy'C
Cuts at: 1391 2325 3332 3340 3758 3938 1391
Size: 934 1007 8 418 180 4072
Fragments arranged by size:
4072 1007 934 418 180 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3243 3845 928
Size: 971 1344 602 3702
Fragments arranged by size:
3702 1344 971 602
BbvCI CC'TCA_GC
Cuts at: 2253 2287 2365 2997 2253
Size: 34 78 632 5875
Fragments arranged by size:
5875 632 78 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2788 2822 3016 3050 4810 4844 1903
Size: 34 851 34 194 34 1760 34 3678
Fragments arranged by size:
3678 1760 851 194 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2656 2739 5230
Size: 107 122 71 631 605 984 83 2491
Cuts at: 5230 136
Size: 1525
Fragments arranged by size:
2491 1525 984 631 605 122 107 83
71
BglII A'GATC_T
Cuts at: 2232 3029 6614 2232
Size: 797 3585 2237
Fragments arranged by size:
3585 2237 797
BlpI GC'TnA_GC
Cuts at: 2319 2324 4036 2319
Size: 5 1712 4902
Fragments arranged by size:
4902 1712 5
Bme1580I G_kGCm'C
Cuts at: 1790 2534 3252 3340 3387 4680 5836 1790
Size: 744 718 88 47 1293 1156 2573
Fragments arranged by size:
2573 1293 1156 744 718 88 47
BmgBI CAC'GTC
Cuts at: 1318 3880 1318
Size: 2562 4057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3568 3653 3675
Size: 416 53 83 399 24 2151 85 22
Cuts at: 3675 4350 5308 442
Size: 675 958 1753
Fragments arranged by size:
2151 1753 958 675 416 399 85 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2365 3547 5279 1239
Size: 1059 67 1182 1732 2579
Fragments arranged by size:
2579 1732 1182 1059 67
Bpu10I CC'TnA_GC
Cuts at: 2253 2287 2365 2997 2253
Size: 34 78 632 5875
Fragments arranged by size:
5875 632 78 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4744 5609 5907 4744
Size: 865 298 5456
Fragments arranged by size:
5456 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2998 3104 5282 882
Size: 617 1499 106 2178 2219
Fragments arranged by size:
2219 2178 1499 617 106
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2780 4426 4808 5443
Size: 53 83 186 936 1247 1646 382 635
Cuts at: 5443 275
Size: 1451
Fragments arranged by size:
1646 1451 1247 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 3551 3581 1178
Size: 30 165 30 630 30 1488 30 4216
Fragments arranged by size:
4216 1488 630 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2569 3559 1186
Size: 1084 299 990 4246
Fragments arranged by size:
4246 1084 990 299
BseYI C'CCAG_C
Cuts at: 2783 3395 5822 6318 2783
Size: 612 2427 496 3084
Fragments arranged by size:
3084 2427 612 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2818 3165 1163
Size: 573 1082 347 4617
Fragments arranged by size:
4617 1082 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3218 4069 4830 5858 1540
Size: 275 186 1217 851 761 1028 2301
Fragments arranged by size:
2301 1217 1028 851 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2264 2325 3387 3716 3758 4680 4765 5836 2264
Size: 61 1062 329 42 922 85 1071 3047
Fragments arranged by size:
3047 1071 1062 922 329 85 61 42
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2325 2534 3252 3332 3340 3387
Size: 399 474 61 209 718 80 8 47
Cuts at: 3387 3716 3758 3938 4680 4765 5836 1391
Size: 329 42 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 718 474 399 329 209
180 85 80 61 47 42 8
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 2550 4508 6238 2550
Size: 1958 1730 2931
Fragments arranged by size:
2931 1958 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 3284 3974 844
Size: 1239 1201 690 3489
Fragments arranged by size:
3489 1239 1201 690
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2307 4506 1394
Size: 429 187 297 2199 3507
Fragments arranged by size:
3507 2199 429 297 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2596 3078 5114 5288 65
Size: 1183 1348 482 2036 174 1396
Fragments arranged by size:
2036 1396 1348 1183 482 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2291 2655 3150 3716 5263 5450 1700
Size: 110 481 364 495 566 1547 187 2869
Fragments arranged by size:
2869 1547 566 495 481 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 2569 2766 96
Size: 2473 197 3949
Fragments arranged by size:
3949 2473 197
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 2513 794
Size: 1710 9 4900
Fragments arranged by size:
4900 1710 9
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 2012 1184
Size: 828 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2237 2591 2911 3277 2237
Size: 354 320 366 5579
Fragments arranged by size:
5579 366 354 320
BstYI r'GATC_y
Cuts at: 1972 2232 2865 3029 3949 4316 4701 4718 6170
Size: 260 633 164 920 367 385 17 1452
Cuts at: 6170 6256 6268 6614 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 920 633 385 367 346 260
164 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2457 3479 2457
Size: 1022 5597
BtgI C'CryG_G
Cuts at: 513 1065 2626 2713 3282 513
Size: 552 1561 87 569 3850
Fragments arranged by size:
3850 1561 569 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4929 4949 4135
Size: 794 20 5805
Fragments arranged by size:
5805 794 20
ClaI AT'CG_AT
Cuts at: 1554 3498 1554
Size: 1944 4675
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 2796 3131 2796
Size: 335 6284
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 2841 2913
Size: 54 1425 50 275 18 886 125 72
Cuts at: 2913 3279 4066 4955 6584 8
Size: 366 787 889 1629 43
Fragments arranged by size:
1629 1425 889 886 787 366 275 125
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2314 2488 3627 3704 4549 1353
Size: 837 124 174 1139 77 845 3423
Fragments arranged by size:
3423 1139 845 837 174 124 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 3756 2323
Size: 1433 5186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2365 3255 3547 4682 5279 6066
Size: 393 666 67 890 292 1135 597 787
Cuts at: 6066 6334 1239
Size: 268 1524
Fragments arranged by size:
1524 1135 890 787 666 597 393 292
268 67
EcoO109I rG'GnC_Cy
Cuts at: 2812 3336 3392 3847 2812
Size: 524 56 455 5584
Fragments arranged by size:
5584 524 455 56
EcoRI G'AATT_C
Cuts at: 2669 3928 4306 2669
Size: 1259 378 4982
Fragments arranged by size:
4982 1259 378
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 5766 1678
Size: 4088 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2586 2602 2618
Size: 32 135 32 773 32 209 16 16
Cuts at: 2618 2634 2866 2898 2997 3029 5260 5292 5334
Size: 16 232 32 99 32 2231 32 42
Cuts at: 5334 5366 1373
Size: 32 2626
Fragments arranged by size:
2626 2231 773 232 209 135 99 42
32 32 32 32 32 32 32 16
16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 3430 3963 4209 669
Size: 988 281 667 825 533 246 3079
Fragments arranged by size:
3079 988 825 667 533 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2718 2915 3281 10
Size: 54 1425 343 886 197 366 3348
Fragments arranged by size:
3348 1425 886 366 343 197 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2909 2983 3323 4577 4936 5095 518
Size: 648 1743 74 340 1254 359 159 2042
Fragments arranged by size:
2042 1743 1254 648 359 340 159 74
MspA1I CmG'CkG
Cuts at: 1606 2318 2567 4035 4712 5860 6105 1606
Size: 712 249 1468 677 1148 245 2120
Fragments arranged by size:
2120 1468 1148 712 677 249 245
NaeI GCC'GGC
Cuts at: 2657 2657
Size: 6619
NcoI C'CATG_G
Cuts at: 513 1065 2626 2713 3282 513
Size: 552 1561 87 569 3850
Fragments arranged by size:
3850 1561 569 552 87
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NgoMIV G'CCGG_C
Cuts at: 2655 2655
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 2431 3977 5522 1132
Size: 97 1202 1546 1545 2229
Fragments arranged by size:
2229 1546 1545 1202 97
PasI CC'CwG_GG
Cuts at: 1348 2584 3332 1348
Size: 1236 748 4635
Fragments arranged by size:
4635 1236 748
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3131 3534 1108
Size: 2023 403 4193
Fragments arranged by size:
4193 2023 403
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2033 2065 4688 4720 6201 6233 2033
Size: 32 2623 32 1481 32 2419
Fragments arranged by size:
2623 2419 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2812 2812
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 3336 3336
Size: 6619
PstI C_TGCA'G
Cuts at: 830 2133 3855 3971 830
Size: 1303 1722 116 3478
Fragments arranged by size:
3478 1722 1303 116
PvuI CG_AT'CG
Cuts at: 2001 3218 2001
Size: 1217 5402
PvuII CAG'CTG
Cuts at: 2318 2567 2318
Size: 249 6370
SacI G_AGCT'C
Cuts at: 2325 3758 2325
Size: 1433 5186
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SapI GCTCTTCn'nnn_
Cuts at: 2314 2488 3704 2314
Size: 174 1216 5229
Fragments arranged by size:
5229 1216 174
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 3578 4867 6579 3578
Size: 1289 1712 3618
Fragments arranged by size:
3618 1712 1289
SexAI A'CCwGG_T
Cuts at: 2178 2178
Size: 6619
SfcI C'TryA_G
Cuts at: 826 1045 2129 2635 3851 3967 5102 5783 5974
Size: 219 1084 506 1216 116 1135 681 191
Cuts at: 5974 826
Size: 1471
Fragments arranged by size:
1471 1216 1135 1084 681 506 219 191
116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3946 1872
Size: 2074 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4723 5624 5886 820
Size: 197 926 2780 901 262 1553
Fragments arranged by size:
2780 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 2106 1517
Size: 589 6030
StyI C'CwwG_G
Cuts at: 513 1065 2626 2713 2724 2870 3282 3903 4058
Size: 552 1561 87 11 146 412 621 155
Cuts at: 4058 513
Size: 3074
Fragments arranged by size:
3074 1561 621 552 412 155 146 87
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 4646 4663 4816 5001 5446 325
Size: 1255 2031 1035 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 2766 3576
Size: 274 80 33 51 1443 592 197 810
Cuts at: 3576 4865 6577 96
Size: 1289 1712 138
Fragments arranged by size:
1712 1443 1289 810 592 274 197 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3679 3711 6579 6611 3679
Size: 32 2868 32 3687
Fragments arranged by size:
3687 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3944 1870
Size: 2074 4545
XmnI GAAnn'nnTTC
Cuts at: 2281 4748 2281
Size: 2467 4152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI