Restriction map for
pFN21AA0569
(Circular) (Six-base) MAPSORT of: pFN21AA0569.seq Check: 657 from: 1 to: 8347
pFN21AA0569 8347 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2913 2913
Size: 8347
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6157 278
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4095 5690 1937
Size: 2158 1595 4594
Fragments arranged by size:
4594 2158 1595
Acc65I G'GTAC_C
Cuts at: 5668 5668
Size: 8347
AclI AA'CG_TT
Cuts at: 1563 2956 3805 6474 6847 1563
Size: 1393 849 2669 373 3063
Fragments arranged by size:
3063 2669 1393 849 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 2702 3465 6410 7794 8062 1632
Size: 857 213 763 2945 1384 268 1917
Fragments arranged by size:
2945 1917 1384 857 763 268 213
AfeI AGC'GCT
Cuts at: 2669 5192 2669
Size: 2523 5824
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8150
AflIII A'CryG_T
Cuts at: 1203 2221 2748 3969 4920 7246 8223 1203
Size: 1018 527 1221 951 2326 977 1327
Fragments arranged by size:
2326 1327 1221 1018 977 951 527
AgeI A'CCGG_T
Cuts at: 7178 7178
Size: 8347
AhdI GACnn_n'nnGTC
Cuts at: 3970 4921 7076 3970
Size: 951 2155 5241
Fragments arranged by size:
5241 2155 951
AleI CACnn'nnGTG
Cuts at: 1166 2919 3321 4251 5086 1166
Size: 1753 402 930 835 4427
Fragments arranged by size:
4427 1753 930 835 402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8347
AlwNI CAG_nnn'CTG
Cuts at: 2111 6090 7662 2111
Size: 3979 1572 2796
Fragments arranged by size:
3979 2796 1572
ApaLI G'TGCA_C
Cuts at: 6404 7560 6404
Size: 1156 7191
ApoI r'AATT_y
Cuts at: 1493 2910 3450 4032 4061 4108 4607 4615 5656
Size: 1417 540 582 29 47 499 8 1041
Cuts at: 5656 5882 6034 8143 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1417 1041 582 540 499 226
152 47 29 8
AseI AT'TA_AT
Cuts at: 160 1032 6901 160
Size: 872 5869 1606
Fragments arranged by size:
5869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 5152 5203 5672 1870
Size: 73 828 2381 51 469 4545
Fragments arranged by size:
4545 2381 828 469 73 51
BamHI G'GATC_C
Cuts at: 3481 5527 5677 6044 3481
Size: 2046 150 367 5784
Fragments arranged by size:
5784 2046 367 150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3563 5668 7123 618
Size: 325 217 627 1776 2105 1455 1842
Fragments arranged by size:
2105 1842 1776 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4011 4437 5210 5666 1391
Size: 2620 426 773 456 4072
Fragments arranged by size:
4072 2620 773 456 426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 2355 3533 4988 5631 928
Size: 971 234 222 1178 1455 643 3644
Fragments arranged by size:
3644 1455 1178 971 643 234 222
BbvCI CC'TCA_GC
Cuts at: 2145 2145
Size: 8347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2462 2496 2752 2786 6538 6572 1903
Size: 34 525 34 256 34 3752 34 3678
Fragments arranged by size:
3752 3678 525 256 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5515 6239 7449 5515
Size: 724 1210 6413
Fragments arranged by size:
6413 1210 724
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8347
BfrBI ATG'CAT
Cuts at: 5003 5003
Size: 8347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6958 136
Size: 107 122 71 631 605 5286 1525
Fragments arranged by size:
5286 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8342 8342
Size: 8347
BlpI GC'TnA_GC
Cuts at: 4443 5764 4443
Size: 1321 7026
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2942 5304 6408 7564 1790
Size: 412 740 2362 1104 1156 2573
Fragments arranged by size:
2573 2362 1156 1104 740 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8347
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3100 4643 6078
Size: 416 53 83 399 24 1683 1543 1435
Cuts at: 6078 7036 442
Size: 958 1753
Fragments arranged by size:
1753 1683 1543 1435 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2250 4030 4365 2250
Size: 1780 335 6232
Fragments arranged by size:
6232 1780 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2598 3608 4321 4421 4848 5580 7007
Size: 1244 115 1010 713 100 427 732 1427
Cuts at: 7007 1239
Size: 2579
Fragments arranged by size:
2579 1427 1244 1010 732 713 427 115
100
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 8347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 5125 6472 7337 7635 3628
Size: 1497 1347 865 298 4340
Fragments arranged by size:
4340 1497 1347 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4200 7010 882
Size: 617 2701 2810 2219
Fragments arranged by size:
2810 2701 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3683 6138 493
Size: 711 1020 1459 2455 2702
Fragments arranged by size:
2702 2455 1459 1020 711
BsaBI GATnn'nnATC
Cuts at: 5526 8341 5526
Size: 2815 5532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6154 6536 7171 275
Size: 53 83 186 936 4621 382 635 1451
Fragments arranged by size:
4621 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3544 6780 7178 7452 7599 8010 3544
Size: 3236 398 274 147 411 3881
Fragments arranged by size:
3881 3236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3873 3903 4339 4369 4937
Size: 30 165 30 2470 30 436 30 568
Cuts at: 4937 4967 1178
Size: 30 4558
Fragments arranged by size:
4558 2470 568 436 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2561 3896 4263 4445 5276 5393 5492
Size: 1324 51 1335 367 182 831 117 99
Cuts at: 5492 5501 1186
Size: 9 4032
Fragments arranged by size:
4032 1335 1324 831 367 182 117 99
51 9
BseYI C'CCAG_C
Cuts at: 2766 5455 7550 8046 2766
Size: 2689 2095 496 3067
Fragments arranged by size:
3067 2689 2095 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7774
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 5797 6558 7586 1540
Size: 275 186 655 3141 761 1028 2301
Fragments arranged by size:
3141 2301 1028 761 655 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 4437 4912 5139 5424 6408 6493 7564 2691
Size: 1746 475 227 285 984 85 1071 3474
Fragments arranged by size:
3474 1746 1071 984 475 285 227 85
BsmI GAATG_Cn'
Cuts at: 2419 5858 5951 2419
Size: 3439 93 4815
Fragments arranged by size:
4815 3439 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 8347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2691 2942 4011 4437 4912 5139
Size: 399 412 489 251 1069 426 475 227
Cuts at: 5139 5210 5304 5424 5666 6408 6493 7564 1391
Size: 71 94 120 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 489 475 426 412
399 251 242 227 120 94 85 71
BspEI T'CCGG_A
Cuts at: 8010 8010
Size: 8347
BspHI T'CATG_A
Cuts at: 3853 6236 7966 3853
Size: 2383 1730 4234
Fragments arranged by size:
4234 2383 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 2648 2825 2913 5702 844
Size: 1763 41 177 88 2789 3489
Fragments arranged by size:
3489 2789 1763 177 88 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 5266 6234 1394
Size: 429 953 2490 968 3507
Fragments arranged by size:
3507 2490 968 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4038 6842 7016 65
Size: 1183 2790 2804 174 1396
Fragments arranged by size:
2804 2790 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6991 7178 1700
Size: 110 5181 187 2869
Fragments arranged by size:
5181 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8347
BssHII G'CGCG_C
Cuts at: 1925 5288 1925
Size: 3363 4984
BssSI C'ACGA_G
Cuts at: 2705 5423 5618 6407 7419 2705
Size: 2718 195 789 1012 3633
Fragments arranged by size:
3633 2718 1012 789 195
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 3992 794
Size: 2024 1174 5149
Fragments arranged by size:
5149 2024 1174
BstBI TT'CG_AA
Cuts at: 7151 7151
Size: 8347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8347
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 4045 2170
Size: 1875 6472
BstYI r'GATC_y
Cuts at: 1972 3481 5527 5677 6044 6429 6446 7898 7984
Size: 1509 2046 150 367 385 17 1452 86
Cuts at: 7984 7996 8342 1972
Size: 12 346 1977
Fragments arranged by size:
2046 1977 1509 1452 385 367 346 150
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2544 3498 4056 5358 2544
Size: 954 558 1302 5533
Fragments arranged by size:
5533 1302 954 558
BtgI C'CryG_G
Cuts at: 513 1065 5571 513
Size: 552 4506 3289
Fragments arranged by size:
4506 3289 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2487 487
Size: 683 56 1261 6347
Fragments arranged by size:
6347 1261 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 5863 6657 6677 2518
Size: 3345 794 20 4188
Fragments arranged by size:
4188 3345 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8312
DraI TTT'AAA
Cuts at: 5651 5651
Size: 8347
DraIII CAC_nnn'GTG
Cuts at: 2862 2862
Size: 8347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7354 809
Size: 507 6038 1802
Fragments arranged by size:
6038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 5794 6683
Size: 54 1425 50 275 18 1198 2766 889
Cuts at: 6683 8312 8
Size: 1629 43
Fragments arranged by size:
2766 1629 1425 1198 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5794 1537
Size: 275 3982 4090
Fragments arranged by size:
4090 3982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 2712 3822 4429 4701 5487 5547 6277
Size: 894 465 1110 607 272 786 60 730
Cuts at: 6277 1353
Size: 3423
Fragments arranged by size:
3423 1110 894 786 730 607 465 272
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 3519 5273 5291 6934 7308
Size: 1064 138 450 1480 1754 18 1643 374
Cuts at: 7308 7454 387
Size: 146 1280
Fragments arranged by size:
1754 1643 1480 1280 1064 450 374 146
138 18
EcoICRI GAG'CTC
Cuts at: 4435 4435
Size: 8347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2489 2598 2702 3465 3608 4321
Size: 393 851 6 109 104 763 143 713
Cuts at: 4321 4421 4848 5580 6410 7007 7794 8062 1239
Size: 100 427 732 830 597 787 268 1524
Fragments arranged by size:
1524 851 830 787 763 732 713 597
427 393 268 143 109 104 100 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4055 4055
Size: 8347
EcoO109I rG'GnC_Cy
Cuts at: 4116 4116
Size: 8347
EcoRI G'AATT_C
Cuts at: 2910 5656 6034 2910
Size: 2746 378 5223
Fragments arranged by size:
5223 2746 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2150 2150
Size: 8347
FspI TGC'GCA
Cuts at: 6853 6853
Size: 8347
HaeII r_GCGC'y
Cuts at: 1678 2671 5194 7494 1678
Size: 993 2523 2300 2531
Fragments arranged by size:
2531 2523 2300 993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 3473 3505 3956
Size: 32 135 32 965 32 904 32 451
Cuts at: 3956 3988 4907 4937 4939 4969 5548 5580 6988
Size: 32 919 30 2 30 579 32 1408
Cuts at: 6988 7020 7062 7094 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1408 965 919 904 579 451 135
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5691 5937 669
Size: 988 281 3753 246 3079
Fragments arranged by size:
3753 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2132 3615 748
Size: 1384 1483 5480
Fragments arranged by size:
5480 1483 1384
HpaI GTT'AAC
Cuts at: 5937 5937
Size: 8347
KpnI G_GTAC'C
Cuts at: 5672 5672
Size: 8347
MfeI C'AATT_G
Cuts at: 5946 5946
Size: 8347
MluI A'CGCG_T
Cuts at: 8223 8223
Size: 8347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 3573 4974 7461 7645 1305
Size: 1797 471 1401 2487 184 2007
Fragments arranged by size:
2487 2007 1797 1401 471 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6525
Fragments arranged by size:
6525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2919 3321 3561 3748 3798 4043 4251
Size: 648 1753 402 240 187 50 245 208
Cuts at: 4251 4545 5086 6305 6664 6823 518
Size: 294 541 1219 359 159 2042
Fragments arranged by size:
2042 1753 1219 648 541 402 359 294
245 240 208 187 159 50
MspA1I CmG'CkG
Cuts at: 1606 4873 5763 6440 7588 7833 1606
Size: 3267 890 677 1148 245 2120
Fragments arranged by size:
3267 2120 1148 890 677 245
NcoI C'CATG_G
Cuts at: 513 1065 5571 513
Size: 552 4506 3289
Fragments arranged by size:
4506 3289 552
NdeI CA'TA_TG
Cuts at: 387 2781 4806 387
Size: 2394 2025 3928
Fragments arranged by size:
3928 2394 2025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8347
NotI GC'GGCC_GC
Cuts at: 5794 5794
Size: 8347
NsiI A_TGCA'T
Cuts at: 5005 5005
Size: 8347
NspI r_CATG'y
Cuts at: 1132 1229 3973 4924 4999 5007 5705 7250 1132
Size: 97 2744 951 75 8 698 1545 2229
Fragments arranged by size:
2744 2229 1545 951 698 97 75 8
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8347
PciI A'CATG_T
Cuts at: 3969 4920 7246 3969
Size: 951 2326 5070
Fragments arranged by size:
5070 2326 951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8347
PfoI T'CCnGG_A
Cuts at: 4337 4337
Size: 8347
PmeI GTTT'AAAC
Cuts at: 5651 5651
Size: 8347
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 8347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6416 6448 7929 7961 6416
Size: 32 1481 32 6802
Fragments arranged by size:
6802 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4116 4116
Size: 8347
PshAI GACnn'nnGTC
Cuts at: 1613 4956 1613
Size: 3343 5004
PsiI TTA'TAA
Cuts at: 5917 5917
Size: 8347
PspXI vC'TCGA_Gb
Cuts at: 2771 2771
Size: 8347
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3375 3407 5321 5353 3375
Size: 32 1914 32 6369
Fragments arranged by size:
6369 1914 32 32
PstI C_TGCA'G
Cuts at: 830 3849 5699 830
Size: 3019 1850 3478
Fragments arranged by size:
3478 3019 1850
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8347
SacI G_AGCT'C
Cuts at: 4437 4437
Size: 8347
SalI G'TCGA_C
Cuts at: 1936 5689 1936
Size: 3753 4594
SapI GCTCTTCn'nnn_
Cuts at: 4701 4701
Size: 8347
SbfI CC_TGCA'GG
Cuts at: 5699 5699
Size: 8347
ScaI AGT'ACT
Cuts at: 4103 6595 8307 4103
Size: 2492 1712 4143
Fragments arranged by size:
4143 2492 1712
SfcI C'TryA_G
Cuts at: 826 1045 2322 2740 2977 3395 3845 4233 5695
Size: 219 1277 418 237 418 450 388 1462
Cuts at: 5695 6830 7511 7702 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1462 1277 1135 681 450 418 418
388 237 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8347
SmaI CCC'GGG
Cuts at: 1872 5674 1872
Size: 3802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2771 3643 5140 6451 7352 7614
Size: 197 926 828 872 1497 1311 901 262
Cuts at: 7614 820
Size: 1553
Fragments arranged by size:
1553 1497 1311 926 901 872 828 262
197
SnaBI TAC'GTA
Cuts at: 493 3683 6138 493
Size: 3190 2455 2702
Fragments arranged by size:
3190 2702 2455
SpeI A'CTAG_T
Cuts at: 152 3239 152
Size: 3087 5260
SphI G_CATG'C
Cuts at: 1132 5705 1132
Size: 4573 3774
SspI AAT'ATT
Cuts at: 5 52 3526 4309 6271 5
Size: 47 3474 783 1962 2081
Fragments arranged by size:
3474 2081 1962 783 47
StuI AGG'CCT
Cuts at: 1517 2873 4929 1517
Size: 1356 2056 4935
Fragments arranged by size:
4935 2056 1356
StyI C'CwwG_G
Cuts at: 513 1065 2560 2827 5571 5786 513
Size: 552 1495 267 2744 215 3074
Fragments arranged by size:
3074 2744 1495 552 267 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 3527 6374 6391 6544 6729 7174
Size: 1255 1200 747 2847 17 153 185 445
Cuts at: 7174 325
Size: 1498
Fragments arranged by size:
2847 1498 1255 1200 747 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3919 3976 4101
Size: 274 80 33 51 1443 1942 57 125
Cuts at: 4101 6593 8305 96
Size: 2492 1712 138
Fragments arranged by size:
2492 1942 1712 1443 274 138 125 80
57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3719 3751 4681 4713 5222 5254 8307 8339 3719
Size: 32 930 32 509 32 3053 32 3727
Fragments arranged by size:
3727 3053 930 509 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8347
XbaI T'CTAG_A
Cuts at: 5683 5683
Size: 8347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 3427 2458
Size: 969 7378
XhoI C'TCGA_G
Cuts at: 1943 2771 1943
Size: 828 7519
XmaI C'CCGG_G
Cuts at: 1870 5672 1870
Size: 3802 4545
XmnI GAAnn'nnTTC
Cuts at: 2957 3771 5108 6476 2957
Size: 814 1337 1368 4828
Fragments arranged by size:
4828 1368 1337 814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6155 276
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI