Restriction map for
pFN21AA0567
(Circular) (Six-base) MAPSORT of: pFN21AA0567.seq Check: 1760 from: 1 to: 7585
pFN21AA0567 7585 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5395 278
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 4651 4928 1937
Size: 1474 1240 277 4594
Fragments arranged by size:
4594 1474 1240 277
Acc65I G'GTAC_C
Cuts at: 4906 4906
Size: 7585
AclI AA'CG_TT
Cuts at: 1563 5712 6085 1563
Size: 4149 373 3063
Fragments arranged by size:
4149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2832 4728 4779 5648 7032 7300 1632
Size: 1010 190 1896 51 869 1384 268 1917
Fragments arranged by size:
1917 1896 1384 1010 869 268 190 51
AflII C'TTAA_G
Cuts at: 820 1017 2783 820
Size: 197 1766 5622
Fragments arranged by size:
5622 1766 197
AflIII A'CryG_T
Cuts at: 1203 2802 2972 3713 4798 6484 7461 1203
Size: 1599 170 741 1085 1686 977 1327
Fragments arranged by size:
1686 1599 1327 1085 977 741 170
AgeI A'CCGG_T
Cuts at: 6416 6416
Size: 7585
AhdI GACnn_n'nnGTC
Cuts at: 6314 6314
Size: 7585
AleI CACnn'nnGTG
Cuts at: 1166 3959 1166
Size: 2793 4792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4245 1184
Size: 3061 4524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4454 4486 4454
Size: 32 7553
AlwNI CAG_nnn'CTG
Cuts at: 5328 6900 5328
Size: 1572 6013
ApaLI G'TGCA_C
Cuts at: 5642 6798 5642
Size: 1156 6429
ApoI r'AATT_y
Cuts at: 1493 2225 2263 3577 3631 3937 4043 4836 4894
Size: 732 38 1314 54 306 106 793 58
Cuts at: 4894 5120 5272 7381 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 793 732 306 226 152
106 58 54 38
AseI AT'TA_AT
Cuts at: 160 1032 3213 6139 160
Size: 872 2181 2926 1606
Fragments arranged by size:
2926 2181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
AvaI C'yCGr_G
Cuts at: 1870 1943 3039 4473 4910 1870
Size: 73 1096 1434 437 4545
Fragments arranged by size:
4545 1434 1096 437 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3662 3695 3662
Size: 33 7552
BamHI G'GATC_C
Cuts at: 4915 5282 4915
Size: 367 7218
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4906 6361 618
Size: 325 217 627 3119 1455 1842
Fragments arranged by size:
3119 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4904 1391
Size: 3513 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 4203 4537 4821 928
Size: 971 591 1713 334 284 3692
Fragments arranged by size:
3692 1713 971 591 334 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5776 5810 1903
Size: 34 3839 34 3678
Fragments arranged by size:
3839 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4270 4501 5477 6687 4270
Size: 231 976 1210 5168
Fragments arranged by size:
5168 1210 976 231
BclI T'GATC_A
Cuts at: 1548 2362 1548
Size: 814 6771
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 6196 136
Size: 107 122 71 631 605 358 4166 1525
Fragments arranged by size:
4166 1525 631 605 358 122 107 71
BglII A'GATC_T
Cuts at: 3067 7580 3067
Size: 4513 3072
BlpI GC'TnA_GC
Cuts at: 5002 5002
Size: 7585
Bme1580I G_kGCm'C
Cuts at: 1790 5646 6802 1790
Size: 3856 1156 2573
Fragments arranged by size:
3856 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5316 6274 442
Size: 416 53 83 399 24 3899 958 1753
Fragments arranged by size:
3899 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2964 6245 1239
Size: 1725 3281 2579
Fragments arranged by size:
3281 2579 1725
Bpu10I CC'TnA_GC
Cuts at: 2531 2531
Size: 7585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 4256 4805 5710 6575 6873 4189
Size: 67 549 905 865 298 4901
Fragments arranged by size:
4901 905 865 549 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3137 4022 6248 882
Size: 617 1638 885 2226 2219
Fragments arranged by size:
2226 2219 1638 885 617
BsaAI yAC'GTr
Cuts at: 493 1204 2216 5376 493
Size: 711 1012 3160 2702
Fragments arranged by size:
3160 2702 1012 711
BsaBI GATnn'nnATC
Cuts at: 4207 7579 4207
Size: 3372 4213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5392 5774 6409 275
Size: 53 83 186 936 3859 382 635 1451
Fragments arranged by size:
3859 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2383 2566 3771 4455 6018 6416 6690 6837 7248
Size: 183 1205 684 1563 398 274 147 411
Cuts at: 7248 2383
Size: 2720
Fragments arranged by size:
2720 1563 1205 684 411 398 274 183
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2962 2992 4382 4412 4470
Size: 30 165 30 1559 30 1390 30 58
Cuts at: 4470 4500 1178
Size: 30 4263
Fragments arranged by size:
4263 1559 1390 165 58 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4427 4491 1186
Size: 3241 64 4280
Fragments arranged by size:
4280 3241 64
BseYI C'CCAG_C
Cuts at: 4420 6788 7284 4420
Size: 2368 496 4721
Fragments arranged by size:
4721 2368 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 1163
Size: 573 921 6091
Fragments arranged by size:
6091 921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5035 5796 6824 1540
Size: 275 186 3034 761 1028 2301
Fragments arranged by size:
3034 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4419 5646 5731 6802 4419
Size: 1227 85 1071 5202
Fragments arranged by size:
5202 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 2766 4628 5096 5189 2766
Size: 1862 468 93 5162
Fragments arranged by size:
5162 1862 468 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4274 4274
Size: 7585
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4419 4904 5646 5731 6802 1391
Size: 399 2629 485 742 85 1071 2174
Fragments arranged by size:
2629 2174 1071 742 485 399 85
BspEI T'CCGG_A
Cuts at: 2566 4455 7248 2566
Size: 1889 2793 2903
Fragments arranged by size:
2903 2793 1889
BspHI T'CATG_A
Cuts at: 5474 7204 5474
Size: 1730 5855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4940 844
Size: 4096 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5472 1394
Size: 429 3649 3507
Fragments arranged by size:
3649 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4633 6080 6254 65
Size: 1183 2981 404 1447 174 1396
Fragments arranged by size:
2981 1447 1396 1183 404 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6229 6416 1700
Size: 110 4419 187 2869
Fragments arranged by size:
4419 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7585
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7585
BssSI C'ACGA_G
Cuts at: 5645 6657 5645
Size: 1012 6573
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7585
BstBI TT'CG_AA
Cuts at: 3100 6389 3100
Size: 3289 4296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7585
BstXI CCAn_nnnn'nTGG
Cuts at: 3849 3849
Size: 7585
BstYI r'GATC_y
Cuts at: 1972 2600 2954 3067 3320 4489 4915 5282 5667
Size: 628 354 113 253 1169 426 367 385
Cuts at: 5667 5684 7136 7222 7234 7580 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1169 628 426 385 367 354
346 253 113 86 17 12
BstZ17I GTA'TAC
Cuts at: 4652 4652
Size: 7585
Bsu36I CC'TnA_GG
Cuts at: 2557 2557
Size: 7585
BtgI C'CryG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 5228
Fragments arranged by size:
5228 1805 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6846
Fragments arranged by size:
6846 683 56
BtsI GCAGTG_nn'
Cuts at: 5101 5895 5915 5101
Size: 794 20 6771
Fragments arranged by size:
6771 794 20
ClaI AT'CG_AT
Cuts at: 1554 2445 1554
Size: 891 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3857 3892 529
Size: 35 3293 35 4222
Fragments arranged by size:
4222 3293 35 35
DraI TTT'AAA
Cuts at: 3028 4542 4889 3028
Size: 1514 347 5724
Fragments arranged by size:
5724 1514 347
DraIII CAC_nnn'GTG
Cuts at: 3016 3619 3016
Size: 603 6982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6592 809
Size: 507 5276 1802
Fragments arranged by size:
5276 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2270 5032 5921
Size: 54 1425 50 275 18 440 2762 889
Cuts at: 5921 7550 8
Size: 1629 43
Fragments arranged by size:
2762 1629 1425 889 440 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5032 1537
Size: 275 3220 4090
Fragments arranged by size:
4090 3220 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2197 3479 3558 4245 4570 4875 5515 1353
Size: 844 1282 79 687 325 305 640 3423
Fragments arranged by size:
3423 1282 844 687 640 325 305 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4826 6172 6546 6692 387
Size: 1064 138 3237 1346 374 146 1280
Fragments arranged by size:
3237 1346 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2642 2832 2964 4728 4779 5648 6245
Size: 393 1010 190 132 1764 51 869 597
Cuts at: 6245 7032 7300 1239
Size: 787 268 1524
Fragments arranged by size:
1764 1524 1010 869 787 597 393 268
190 132 51
EcoO109I rG'GnC_Cy
Cuts at: 3005 3167 3005
Size: 162 7423
EcoRI G'AATT_C
Cuts at: 3577 3937 4894 5272 3577
Size: 360 957 378 5890
Fragments arranged by size:
5890 957 378 360
EcoRV GAT'ATC
Cuts at: 3967 3967
Size: 7585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3086 4865 3086
Size: 1779 5806
FspI TGC'GCA
Cuts at: 3927 6091 3927
Size: 2164 5421
HaeII r_GCGC'y
Cuts at: 1678 6732 1678
Size: 5054 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 2585 2617 2813
Size: 32 135 32 775 32 206 32 196
Cuts at: 2813 2845 2962 2989 2994 3021 3134 3166 6226
Size: 32 117 27 5 27 113 32 3060
Cuts at: 6226 6258 6300 6332 1373
Size: 32 42 32 2626
Fragments arranged by size:
3060 2626 775 206 196 135 117 113
42 32 32 32 32 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3196 4929 5175 669
Size: 988 281 1258 1733 246 3079
Fragments arranged by size:
3079 1733 1258 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2150 2780 3273 3486 3971 4495 748
Size: 1402 630 493 213 485 524 3838
Fragments arranged by size:
3838 1402 630 524 493 485 213
HpaI GTT'AAC
Cuts at: 5175 5175
Size: 7585
KpnI G_GTAC'C
Cuts at: 4910 4910
Size: 7585
MfeI C'AATT_G
Cuts at: 4290 5184 4290
Size: 894 6691
MluI A'CGCG_T
Cuts at: 4798 7461 4798
Size: 2663 4922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2866 2898 4599 6699 6883 1305
Size: 1561 32 1701 2100 184 2007
Fragments arranged by size:
2100 2007 1701 1561 184 32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2272 10
Size: 54 1425 343 440 5323
Fragments arranged by size:
5323 1425 440 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 5543 5902 6061 518
Size: 648 2793 1584 359 159 2042
Fragments arranged by size:
2793 2042 1584 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 3923 5001 5678 6826 7071 1606
Size: 421 1896 1078 677 1148 245 2120
Fragments arranged by size:
2120 1896 1148 1078 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7033
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7585
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7585
NotI GC'GGCC_GC
Cuts at: 5032 5032
Size: 7585
NspI r_CATG'y
Cuts at: 1132 1229 2806 3283 3604 4663 4943 6488 1132
Size: 97 1577 477 321 1059 280 1545 2229
Fragments arranged by size:
2229 1577 1545 1059 477 321 280 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7585
PciI A'CATG_T
Cuts at: 2802 6484 2802
Size: 3682 3903
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7585
PmeI GTTT'AAAC
Cuts at: 4889 4889
Size: 7585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 7167 7199 5654
Size: 32 1481 32 6040
Fragments arranged by size:
6040 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3005 3005
Size: 7585
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7585
PsiI TTA'TAA
Cuts at: 2850 5155 2850
Size: 2305 5280
PstI C_TGCA'G
Cuts at: 830 4263 4937 830
Size: 3433 674 3478
Fragments arranged by size:
3478 3433 674
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7585
PvuII CAG'CTG
Cuts at: 3923 3923
Size: 7585
SalI G'TCGA_C
Cuts at: 1936 4927 1936
Size: 2991 4594
SapI GCTCTTCn'nnn_
Cuts at: 3479 4875 3479
Size: 1396 6189
SbfI CC_TGCA'GG
Cuts at: 4937 4937
Size: 7585
ScaI AGT'ACT
Cuts at: 5833 7545 5833
Size: 1712 5873
SexAI A'CCwGG_T
Cuts at: 3202 3202
Size: 7585
SfcI C'TryA_G
Cuts at: 826 1045 3176 4259 4933 6068 6749 6940 826
Size: 219 2131 1083 674 1135 681 191 1471
Fragments arranged by size:
2131 1471 1135 1083 681 674 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 7585
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7585
SmaI CCC'GGG
Cuts at: 1872 4912 1872
Size: 3040 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 4168 4271 4820 5689 6590
Size: 197 926 840 1385 103 549 869 901
Cuts at: 6590 6852 820
Size: 262 1553
Fragments arranged by size:
1553 1385 926 901 869 840 549 262
197 103
SnaBI TAC'GTA
Cuts at: 493 2216 5376 493
Size: 1723 3160 2702
Fragments arranged by size:
3160 2702 1723
SpeI A'CTAG_T
Cuts at: 152 2909 152
Size: 2757 4828
SphI G_CATG'C
Cuts at: 1132 3604 4663 4943 1132
Size: 2472 1059 280 3774
Fragments arranged by size:
3774 2472 1059 280
SspI AAT'ATT
Cuts at: 5 52 2943 5509 5
Size: 47 2891 2566 2081
Fragments arranged by size:
2891 2566 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7585
StyI C'CwwG_G
Cuts at: 513 1065 5024 513
Size: 552 3959 3074
Fragments arranged by size:
3959 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4215 4434 5612 5629 5782 5967 6412
Size: 1255 2635 219 1178 17 153 185 445
Cuts at: 6412 325
Size: 1498
Fragments arranged by size:
2635 1498 1255 1178 445 219 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5831 7543 96
Size: 274 80 33 51 1443 3854 1712 138
Fragments arranged by size:
3854 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7545 7577 7545
Size: 32 7553
Tth111I GACn'n_nGTC
Cuts at: 1316 2016 3356 1316
Size: 700 1340 5545
Fragments arranged by size:
5545 1340 700
XbaI T'CTAG_A
Cuts at: 4921 4921
Size: 7585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3412 4384 3412
Size: 972 6613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7585
XmaI C'CCGG_G
Cuts at: 1870 4910 1870
Size: 3040 4545
XmnI GAAnn'nnTTC
Cuts at: 2667 3861 4869 5714 2667
Size: 1194 1008 845 4538
Fragments arranged by size:
4538 1194 1008 845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5393 276
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfoI SrfI
SwaI