Restriction map for
pFN21AA0560
(Circular) (Six-base) MAPSORT of: pFN21AA0560.seq Check: 5773 from: 1 to: 9160
pFN21AA0560 9160 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6175 6175
Size: 9160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6970 278
Size: 53 83 186 936 5434 2468
Fragments arranged by size:
5434 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 6099 6503 1937
Size: 544 3618 404 4594
Fragments arranged by size:
4594 3618 544 404
Acc65I G'GTAC_C
Cuts at: 4051 6481 4051
Size: 2430 6730
AclI AA'CG_TT
Cuts at: 1563 7287 7660 1563
Size: 5724 373 3063
Fragments arranged by size:
5724 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5007 7223 8607 8875 1632
Size: 3375 2216 1384 268 1917
Fragments arranged by size:
3375 2216 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8963
AflIII A'CryG_T
Cuts at: 1203 3820 5643 6105 8059 9036 1203
Size: 2617 1823 462 1954 977 1327
Fragments arranged by size:
2617 1954 1823 1327 977 462
AgeI A'CCGG_T
Cuts at: 7991 7991
Size: 9160
AhdI GACnn_n'nnGTC
Cuts at: 7889 7889
Size: 9160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5449 1184
Size: 4265 4895
AlwNI CAG_nnn'CTG
Cuts at: 2015 3786 5206 6903 8475 2015
Size: 1771 1420 1697 1572 2700
Fragments arranged by size:
2700 1771 1697 1572 1420
ApaI G_GGCC'C
Cuts at: 4475 4475
Size: 9160
ApaLI G'TGCA_C
Cuts at: 4101 7217 8373 4101
Size: 3116 1156 4888
Fragments arranged by size:
4888 3116 1156
ApoI r'AATT_y
Cuts at: 1493 2207 2414 2588 2921 3206 3246 3370 3444
Size: 714 207 174 333 285 40 124 74
Cuts at: 3444 3792 5013 5033 6469 6695 6847 8956 1493
Size: 348 1221 20 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 1221 714 348 333 285
226 207 174 152 124 74 40 20
AseI AT'TA_AT
Cuts at: 160 1032 2943 5544 7714 160
Size: 872 1911 2601 2170 1606
Fragments arranged by size:
2601 2170 1911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9160
AvaI C'yCGr_G
Cuts at: 1870 1943 3087 3603 6485 1870
Size: 73 1144 516 2882 4545
Fragments arranged by size:
4545 2882 1144 516 73
BamHI G'GATC_C
Cuts at: 2626 3892 6490 6857 2626
Size: 1266 2598 367 4929
Fragments arranged by size:
4929 2598 1266 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4051 6481 7936 618
Size: 325 217 627 2264 2430 1455 1842
Fragments arranged by size:
2430 2264 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2101 4347 4475 6479 1391
Size: 710 2246 128 2004 4072
Fragments arranged by size:
4072 2246 2004 710 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3192 3662 4224 4317 4977 5767 928
Size: 971 1293 470 562 93 660 790 4321
Fragments arranged by size:
4321 1293 971 790 660 562 470 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4092 4126 5696 5730 7351 7385 1903
Size: 34 2155 34 1570 34 1621 34 3678
Fragments arranged by size:
3678 2155 1621 1570 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3294 5935 7052 8262 3294
Size: 2641 1117 1210 4192
Fragments arranged by size:
4192 2641 1210 1117
BclI T'GATC_A
Cuts at: 1548 2455 1548
Size: 907 8253
BfrBI ATG'CAT
Cuts at: 6115 6115
Size: 9160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7771 136
Size: 107 122 71 631 605 6099 1525
Fragments arranged by size:
6099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4958 5072 9155 4958
Size: 114 4083 4963
Fragments arranged by size:
4963 4083 114
BlpI GC'TnA_GC
Cuts at: 6577 6577
Size: 9160
Bme1580I G_kGCm'C
Cuts at: 1790 4105 4475 7221 8377 1790
Size: 2315 370 2746 1156 2573
Fragments arranged by size:
2746 2573 2315 1156 370
BmgBI CAC'GTC
Cuts at: 1318 3821 1318
Size: 2503 6657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 2104 6255
Size: 416 53 83 399 24 654 33 4151
Cuts at: 6255 6315 6891 7849 442
Size: 60 576 958 1753
Fragments arranged by size:
4151 1753 958 654 576 416 399 83
60 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5945 6066 5945
Size: 121 9039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3360 4034 4682 5228 5292 5940 6279 6330
Size: 2121 674 648 546 64 648 339 51
Cuts at: 6330 7820 1239
Size: 1490 2579
Fragments arranged by size:
2579 2121 1490 674 648 648 546 339
64 51
Bpu10I CC'TnA_GC
Cuts at: 2577 3404 2577
Size: 827 8333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5048 5394 7285 8150 8448 5048
Size: 346 1891 865 298 5760
Fragments arranged by size:
5760 1891 865 346 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3739 6037 6365 6446 7823 882
Size: 617 2240 2298 328 81 1377 2219
Fragments arranged by size:
2298 2240 2219 1377 617 328 81
BsaAI yAC'GTr
Cuts at: 493 1204 6951 493
Size: 711 5747 2702
Fragments arranged by size:
5747 2702 711
BsaBI GATnn'nnATC
Cuts at: 2889 9154 2889
Size: 6265 2895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6967 7349 7984 275
Size: 53 83 186 936 5434 382 635 1451
Fragments arranged by size:
5434 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3738 7593 7991 8265 8412 8823 3738
Size: 3855 398 274 147 411 4075
Fragments arranged by size:
4075 3855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6328 6358 1178
Size: 30 165 30 4925 30 3980
Fragments arranged by size:
4925 3980 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2674 3757 3876 3991 4926 1186
Size: 1488 1083 119 115 935 5420
Fragments arranged by size:
5420 1488 1083 935 119 115
BseYI C'CCAG_C
Cuts at: 2901 3537 4833 8363 8859 2901
Size: 636 1296 3530 496 3202
Fragments arranged by size:
3530 3202 1296 636 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3633 4049 5504 6387 1163
Size: 573 1897 416 1455 883 3936
Fragments arranged by size:
3936 1897 1455 883 573 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6610 7371 8399 1540
Size: 275 186 4609 761 1028 2301
Fragments arranged by size:
4609 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4105 7221 7306 8377 4105
Size: 3116 85 1071 4888
Fragments arranged by size:
4888 3116 1071 85
BsiWI C'GTAC_G
Cuts at: 3703 3703
Size: 9160
BsmI GAATG_Cn'
Cuts at: 4449 6671 6764 4449
Size: 2222 93 6845
Fragments arranged by size:
6845 2222 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 4105 4347 4475 6479 7221 7306
Size: 399 311 2004 242 128 2004 742 85
Cuts at: 7306 8377 1391
Size: 1071 2174
Fragments arranged by size:
2174 2004 2004 1071 742 399 311 242
128 85
BspEI T'CCGG_A
Cuts at: 8823 8823
Size: 9160
BspHI T'CATG_A
Cuts at: 2425 3235 3391 6052 7049 8779 2425
Size: 810 156 2661 997 1730 2806
Fragments arranged by size:
2806 2661 1730 997 810 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3408 3751 4674 6175 6515 844
Size: 2564 343 923 1501 340 3489
Fragments arranged by size:
3489 2564 1501 923 343 340
BsrBI CCG'CTC
Cuts at: 1394 1823 7047 1394
Size: 429 5224 3507
Fragments arranged by size:
5224 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2458 4874 7655 7829 65
Size: 1183 1210 2416 2781 174 1396
Fragments arranged by size:
2781 2416 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6406 7804 7991 1700
Size: 110 4596 1398 187 2869
Fragments arranged by size:
4596 2869 1398 187 110
BsrGI T'GTAC_A
Cuts at: 96 5640 6108 96
Size: 5544 468 3148
Fragments arranged by size:
5544 3148 468
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9160
BssSI C'ACGA_G
Cuts at: 7220 8232 7220
Size: 1012 8148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 5491 794
Size: 1221 3476 4463
Fragments arranged by size:
4463 3476 1221
BstBI TT'CG_AA
Cuts at: 4112 5056 7964 4112
Size: 944 2908 5308
Fragments arranged by size:
5308 2908 944
BstEII G'GTnAC_C
Cuts at: 1184 5657 1184
Size: 4473 4687
BstXI CCAn_nnnn'nTGG
Cuts at: 5845 5845
Size: 9160
BstYI r'GATC_y
Cuts at: 1972 2626 3892 4958 5072 6490 6857 7242 7259
Size: 654 1266 1066 114 1418 367 385 17
Cuts at: 7259 8711 8797 8809 9155 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1418 1266 1066 654 385 367
346 114 86 17 12
BstZ17I GTA'TAC
Cuts at: 6100 6100
Size: 9160
Bsu36I CC'TnA_GG
Cuts at: 2253 5247 6426 2253
Size: 2994 1179 4987
Fragments arranged by size:
4987 2994 1179
BtgI C'CryG_G
Cuts at: 513 1065 2004 3485 513
Size: 552 939 1481 6188
Fragments arranged by size:
6188 1481 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8421
Fragments arranged by size:
8421 683 56
BtsI GCAGTG_nn'
Cuts at: 5850 6676 7470 7490 5850
Size: 826 794 20 7520
Fragments arranged by size:
7520 826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9125
DraI TTT'AAA
Cuts at: 2374 4176 4951 6464 2374
Size: 1802 775 1513 5070
Fragments arranged by size:
5070 1802 1513 775
DraIII CAC_nnn'GTG
Cuts at: 3142 3142
Size: 9160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8167 809
Size: 507 6851 1802
Fragments arranged by size:
6851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2887 3570 3596
Size: 54 1425 50 275 18 1057 683 26
Cuts at: 3596 5698 5880 6607 7496 9125 8
Size: 2102 182 727 889 1629 43
Fragments arranged by size:
2102 1629 1425 1057 889 727 683 275
182 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 6607 1537
Size: 275 4795 4090
Fragments arranged by size:
4795 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 3177 3363 3393 3425 4650 4687 4833
Size: 1521 303 186 30 32 1225 37 146
Cuts at: 4833 5184 5235 5366 5936 6174 6222 6444 7090
Size: 351 51 131 570 238 48 222 646
Cuts at: 7090 1353
Size: 3423
Fragments arranged by size:
3423 1521 1225 646 570 351 303 238
222 186 146 131 51 48 37 32
30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7747 8121 8267 387
Size: 1064 138 6158 374 146 1280
Fragments arranged by size:
6158 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3360 4034 4682 5007 5228 5292 5940
Size: 393 1728 674 648 325 221 64 648
Cuts at: 5940 6279 6330 7223 7820 8607 8875 1239
Size: 339 51 893 597 787 268 1524
Fragments arranged by size:
1728 1524 893 787 674 648 648 597
393 339 325 268 221 64 51
EcoNI CCTnn'n_nnAGG
Cuts at: 4482 4482
Size: 9160
EcoO109I rG'GnC_Cy
Cuts at: 2788 4090 4367 4576 2788
Size: 1302 277 209 7372
Fragments arranged by size:
7372 1302 277 209
EcoRI G'AATT_C
Cuts at: 5033 6469 6847 5033
Size: 1436 378 7346
Fragments arranged by size:
7346 1436 378
EcoRV GAT'ATC
Cuts at: 4072 4072
Size: 9160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3227 3368 3227
Size: 141 9019
FspI TGC'GCA
Cuts at: 2019 7666 2019
Size: 5647 3513
HaeII r_GCGC'y
Cuts at: 1678 2809 4627 8307 1678
Size: 1131 1818 3680 2531
Fragments arranged by size:
3680 2531 1818 1131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2479 2511 3633 3665 5107
Size: 32 135 32 907 32 1122 32 1442
Cuts at: 5107 5139 5275 5307 5895 5927 6032 6064 6118
Size: 32 136 32 588 32 105 32 54
Cuts at: 6118 6150 6422 6454 7801 7833 7875 7907 1373
Size: 32 272 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1442 1347 1122 907 588 272 136
135 105 54 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5398 5801 6504 6750 669
Size: 988 281 3460 403 703 246 3079
Fragments arranged by size:
3460 3079 988 703 403 281 246
HindIII A'AGCT_T
Cuts at: 748 5428 748
Size: 4680 4480
HpaI GTT'AAC
Cuts at: 6750 6750
Size: 9160
KpnI G_GTAC'C
Cuts at: 4055 6485 4055
Size: 2430 6730
MfeI C'AATT_G
Cuts at: 6759 6759
Size: 9160
MluI A'CGCG_T
Cuts at: 9036 9036
Size: 9160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3311 4542 5362 5414 6029 6290 8274 8458
Size: 2006 1231 820 52 615 261 1984 184
Cuts at: 8458 1305
Size: 2007
Fragments arranged by size:
2007 2006 1984 1231 820 615 261 184
52
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2889 3572 3598 5700 5882
Size: 54 1425 343 1057 683 26 2102 182
Cuts at: 5882 10
Size: 3288
Fragments arranged by size:
3288 2102 1425 1057 683 343 182 54
26
MslI CAynn'nnrTG
Cuts at: 518 1166 2751 5183 6110 7118 7477 7636 518
Size: 648 1585 2432 927 1008 359 159 2042
Fragments arranged by size:
2432 2042 1585 1008 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2542 6279 6576 7253 8401 8646 1606
Size: 936 3737 297 677 1148 245 2120
Fragments arranged by size:
3737 2120 1148 936 677 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3485 513
Size: 552 939 1481 6188
Fragments arranged by size:
6188 1481 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9160
NotI GC'GGCC_GC
Cuts at: 6607 6607
Size: 9160
NruI TCG'CGA
Cuts at: 5718 5718
Size: 9160
NsiI A_TGCA'T
Cuts at: 6117 6117
Size: 9160
NspI r_CATG'y
Cuts at: 1132 1229 2088 2917 3805 5647 6109 6115 6518
Size: 97 859 829 888 1842 462 6 403
Cuts at: 6518 8063 1132
Size: 1545 2229
Fragments arranged by size:
2229 1842 1545 888 859 829 462 403
97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9160
PciI A'CATG_T
Cuts at: 5643 6105 8059 5643
Size: 462 1954 6744
Fragments arranged by size:
6744 1954 462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 2908 3142 5756 1108
Size: 1734 66 234 2614 4512
Fragments arranged by size:
4512 2614 1734 234 66
PfoI T'CCnGG_A
Cuts at: 2823 4768 2823
Size: 1945 7215
PmeI GTTT'AAAC
Cuts at: 6464 6464
Size: 9160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7229 7261 8742 8774 7229
Size: 32 1481 32 7615
Fragments arranged by size:
7615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4090 4367 4090
Size: 277 8883
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9160
PsiI TTA'TAA
Cuts at: 4380 6730 4380
Size: 2350 6810
PspOMI G'GGCC_C
Cuts at: 4471 4471
Size: 9160
PstI C_TGCA'G
Cuts at: 830 4801 6512 830
Size: 3971 1711 3478
Fragments arranged by size:
3971 3478 1711
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9160
PvuII CAG'CTG
Cuts at: 2542 6279 2542
Size: 3737 5423
SalI G'TCGA_C
Cuts at: 1936 6502 1936
Size: 4566 4594
SanDI GG'GwC_CC
Cuts at: 4090 4090
Size: 9160
SapI GCTCTTCn'nnn_
Cuts at: 3363 4650 3363
Size: 1287 7873
SbfI CC_TGCA'GG
Cuts at: 6512 6512
Size: 9160
ScaI AGT'ACT
Cuts at: 3332 5339 7408 9120 3332
Size: 2007 2069 1712 3372
Fragments arranged by size:
3372 2069 2007 1712
SexAI A'CCwGG_T
Cuts at: 3138 4477 4818 3138
Size: 1339 341 7480
Fragments arranged by size:
7480 1339 341
SfcI C'TryA_G
Cuts at: 826 1045 3014 4212 4797 5237 6508 7643 8324
Size: 219 1969 1198 585 440 1271 1135 681
Cuts at: 8324 8515 826
Size: 191 1471
Fragments arranged by size:
1969 1471 1271 1198 1135 681 585 440
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9160
SmaI CCC'GGG
Cuts at: 1872 6487 1872
Size: 4615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5027 5409 7264 8165 8427 820
Size: 197 926 3084 382 1855 901 262 1553
Fragments arranged by size:
3084 1855 1553 926 901 382 262 197
SnaBI TAC'GTA
Cuts at: 493 6951 493
Size: 6458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9160
SphI G_CATG'C
Cuts at: 1132 3805 6518 1132
Size: 2673 2713 3774
Fragments arranged by size:
3774 2713 2673
SspI AAT'ATT
Cuts at: 5 52 3316 7084 5
Size: 47 3264 3768 2081
Fragments arranged by size:
3768 3264 2081 47
StuI AGG'CCT
Cuts at: 1517 2270 1517
Size: 753 8407
StyI C'CwwG_G
Cuts at: 513 1065 2004 3096 3485 6456 6599 513
Size: 552 939 1092 389 2971 143 3074
Fragments arranged by size:
3074 2971 1092 939 552 389 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7187 7204 7357 7542 7987 325
Size: 1255 5607 17 153 185 445 1498
Fragments arranged by size:
5607 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2500 3330 4871
Size: 274 80 33 51 1443 523 830 1541
Cuts at: 4871 5120 5337 5504 5640 6058 6108 7406 9118
Size: 249 217 167 136 418 50 1298 1712
Cuts at: 9118 96
Size: 138
Fragments arranged by size:
1712 1541 1443 1298 830 523 418 274
249 217 167 138 136 80 51 50
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2696 6328 6360 9120 9152 2664
Size: 32 3632 32 2760 32 2672
Fragments arranged by size:
3632 2760 2672 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5150 1316
Size: 3834 5326
XbaI T'CTAG_A
Cuts at: 2782 3214 5888 6496 2782
Size: 432 2674 608 5446
Fragments arranged by size:
5446 2674 608 432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 6382 2032
Size: 4350 4810
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9160
XmaI C'CCGG_G
Cuts at: 1870 6485 1870
Size: 4615 4545
XmnI GAAnn'nnTTC
Cuts at: 3007 3210 3669 4676 5052 5556 7289 3007
Size: 203 459 1007 376 504 1733 4878
Fragments arranged by size:
4878 1733 1007 504 459 376 203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6968 276
Size: 53 83 186 936 5434 2468
Fragments arranged by size:
5434 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsmBI
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV PacI
PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI
SrfI SwaI