Restriction map for
pFN21AA0555
(Circular) (Six-base) MAPSORT of: pFN21AA0555.seq Check: 8631 from: 1 to: 7102
pFN21AA0555 7102 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 2801 2488
Size: 313 6789
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4912 278
Size: 53 83 186 936 3376 2468
Fragments arranged by size:
3376 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4445 1937
Size: 2508 4594
Acc65I G'GTAC_C
Cuts at: 4423 4423
Size: 7102
AclI AA'CG_TT
Cuts at: 1563 5229 5602 1563
Size: 3666 373 3063
Fragments arranged by size:
3666 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3137 3170 3438 4235 5165 6549 6817
Size: 1073 432 33 268 797 930 1384 268
Cuts at: 6817 1632
Size: 1917
Fragments arranged by size:
1917 1384 1073 930 797 432 268 268
33
AfeI AGC'GCT
Cuts at: 2400 2400
Size: 7102
AflII C'TTAA_G
Cuts at: 820 1017 2453 820
Size: 197 1436 5469
Fragments arranged by size:
5469 1436 197
AflIII A'CryG_T
Cuts at: 1203 2947 6001 6978 1203
Size: 1744 3054 977 1327
Fragments arranged by size:
3054 1744 1327 977
AgeI A'CCGG_T
Cuts at: 3645 5933 3645
Size: 2288 4814
AhdI GACnn_n'nnGTC
Cuts at: 5831 5831
Size: 7102
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7102
AlwNI CAG_nnn'CTG
Cuts at: 4845 6417 4845
Size: 1572 5530
ApaLI G'TGCA_C
Cuts at: 5159 6315 5159
Size: 1156 5946
ApoI r'AATT_y
Cuts at: 1493 2796 3076 3753 3871 4290 4411 4637 4789
Size: 1303 280 677 118 419 121 226 152
Cuts at: 4789 6898 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1303 677 419 280 226 152
121 118
AseI AT'TA_AT
Cuts at: 160 1032 5656 160
Size: 872 4624 1606
Fragments arranged by size:
4624 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2415 4427 1870
Size: 73 96 376 2012 4545
Fragments arranged by size:
4545 2012 376 96 73
BamHI G'GATC_C
Cuts at: 3939 4432 4799 3939
Size: 493 367 6242
Fragments arranged by size:
6242 493 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4423 5878 618
Size: 325 217 627 2636 1455 1842
Fragments arranged by size:
2636 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3222 4269 4421 1391
Size: 1831 1047 152 4072
Fragments arranged by size:
4072 1831 1047 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 2162 2907 3591 928
Size: 971 183 80 745 684 4439
Fragments arranged by size:
4439 971 745 684 183 80
BbvCI CC'TCA_GC
Cuts at: 3960 4181 4217 3960
Size: 221 36 6845
Fragments arranged by size:
6845 221 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5293 5327 1903
Size: 34 3356 34 3678
Fragments arranged by size:
3678 3356 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4994 6204 4994
Size: 1210 5892
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5713 136
Size: 107 122 71 631 605 4041 1525
Fragments arranged by size:
4041 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3789 7097 3789
Size: 3308 3794
BlpI GC'TnA_GC
Cuts at: 4519 4519
Size: 7102
Bme1580I G_kGCm'C
Cuts at: 1790 5163 6319 1790
Size: 3373 1156 2573
Fragments arranged by size:
3373 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4833 5791 442
Size: 416 53 83 399 24 3416 958 1753
Fragments arranged by size:
3416 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3257
Size: 7102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2705 3713 4055 5762 1239
Size: 968 498 1008 342 1707 2579
Fragments arranged by size:
2579 1707 1008 968 498 342
Bpu10I CC'TnA_GC
Cuts at: 3563 3960 4181 4217 3563
Size: 397 221 36 6448
Fragments arranged by size:
6448 397 221 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 2330 3522 5227 6092 6390 2145
Size: 185 1192 1705 865 298 2857
Fragments arranged by size:
2857 1705 1192 865 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 3669 5765 882
Size: 617 1182 988 2096 2219
Fragments arranged by size:
2219 2096 1182 988 617
BsaAI yAC'GTr
Cuts at: 493 1204 2324 4893 493
Size: 711 1120 2569 2702
Fragments arranged by size:
2702 2569 1120 711
BsaBI GATnn'nnATC
Cuts at: 7096 7096
Size: 7102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3669 4909 5291 5926
Size: 53 83 186 936 2136 1240 382 635
Cuts at: 5926 275
Size: 1451
Fragments arranged by size:
2136 1451 1240 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3645 5535 5933 6207 6354 6765 3645
Size: 1890 398 274 147 411 3982
Fragments arranged by size:
3982 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2697 2727 2753 2783 1178
Size: 30 165 30 1294 30 26 30 5497
Fragments arranged by size:
5497 1294 165 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2435 4190 1186
Size: 1069 180 1755 4098
Fragments arranged by size:
4098 1755 1069 180
BseYI C'CCAG_C
Cuts at: 2546 6305 6801 2546
Size: 3759 496 2847
Fragments arranged by size:
3759 2847 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4552 5313 6341 1540
Size: 275 186 2551 761 1028 2301
Fragments arranged by size:
2551 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2365 3222 3784 4269 5163 5248 6319 2365
Size: 857 562 485 894 85 1071 3148
Fragments arranged by size:
3148 1071 894 857 562 485 85
BsmI GAATG_Cn'
Cuts at: 2884 4613 4706 2884
Size: 1729 93 5280
Fragments arranged by size:
5280 1729 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3721 3721
Size: 7102
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2365 3222 3784 4269 4421 5163 5248
Size: 399 575 857 562 485 152 742 85
Cuts at: 5248 6319 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 857 742 575 562 485 399
152 85
BspEI T'CCGG_A
Cuts at: 6765 6765
Size: 7102
BspHI T'CATG_A
Cuts at: 4991 6721 4991
Size: 1730 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 2801 4457 844
Size: 1644 313 1656 3489
Fragments arranged by size:
3489 1656 1644 313
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 4989 1394
Size: 429 791 2375 3507
Fragments arranged by size:
3507 2375 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2975 5597 5771 65
Size: 1183 1727 2622 174 1396
Fragments arranged by size:
2622 1727 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3645 5746 5933 1700
Size: 110 1835 2101 187 2869
Fragments arranged by size:
2869 2101 1835 187 110
BsrGI T'GTAC_A
Cuts at: 96 2704 4110 96
Size: 2608 1406 3088
Fragments arranged by size:
3088 2608 1406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7102
BssSI C'ACGA_G
Cuts at: 2291 5162 6174 2291
Size: 2871 1012 3219
Fragments arranged by size:
3219 2871 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 5690
BstBI TT'CG_AA
Cuts at: 3700 5906 3700
Size: 2206 4896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7102
BstYI r'GATC_y
Cuts at: 1972 2334 3789 3939 4432 4799 5184 5201 6653
Size: 362 1455 150 493 367 385 17 1452
Cuts at: 6653 6739 6751 7097 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1455 1452 493 385 367 362 346
150 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2081 2081
Size: 7102
BtgI C'CryG_G
Cuts at: 513 1065 3348 513
Size: 552 2283 4267
Fragments arranged by size:
4267 2283 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6363
Fragments arranged by size:
6363 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 2827 4618 5412 5432 2386
Size: 441 1791 794 20 4056
Fragments arranged by size:
4056 1791 794 441 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7067
DraI TTT'AAA
Cuts at: 4406 4406
Size: 7102
DraIII CAC_nnn'GTG
Cuts at: 2206 2206
Size: 7102
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6109 809
Size: 507 4793 1802
Fragments arranged by size:
4793 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 3894 4549
Size: 54 1425 50 275 18 657 1407 655
Cuts at: 4549 5438 7067 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1407 889 657 655 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4549 1537
Size: 275 2737 4090
Fragments arranged by size:
4090 2737 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3711 4217 5032 1353
Size: 812 1546 506 815 3423
Fragments arranged by size:
3423 1546 815 812 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5689 6063 6209 387
Size: 1064 138 4100 374 146 1280
Fragments arranged by size:
4100 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3220 4267 3220
Size: 1047 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2705 3137 3170 3438 3713 4055
Size: 393 575 498 432 33 268 275 342
Cuts at: 4055 4235 5165 5762 6549 6817 1239
Size: 180 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 575 498 432 393
342 275 268 268 180 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 3488 2675
Size: 813 6289
EcoO109I rG'GnC_Cy
Cuts at: 2551 3463 2551
Size: 912 6190
EcoRI G'AATT_C
Cuts at: 4290 4411 4789 4290
Size: 121 378 6603
Fragments arranged by size:
6603 378 121
EcoRV GAT'ATC
Cuts at: 3853 3853
Size: 7102
FspI TGC'GCA
Cuts at: 5608 5608
Size: 7102
HaeII r_GCGC'y
Cuts at: 1678 2402 2452 6249 1678
Size: 724 50 3797 2531
Fragments arranged by size:
3797 2531 724 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2551 2555 2583 2587 3308
Size: 32 135 32 979 4 28 4 721
Cuts at: 3308 3329 3340 3361 3495 3527 3589 3621 3716
Size: 21 11 21 134 32 62 32 95
Cuts at: 3716 3748 5743 5775 5817 5849 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 979 721 135 134 95 62
42 32 32 32 32 32 32 32
28 21 21 11 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4446 4692 669
Size: 988 281 2508 246 3079
Fragments arranged by size:
3079 2508 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2141 748
Size: 1393 5709
HpaI GTT'AAC
Cuts at: 4692 4692
Size: 7102
KpnI G_GTAC'C
Cuts at: 4427 4427
Size: 7102
MfeI C'AATT_G
Cuts at: 4701 4701
Size: 7102
MluI A'CGCG_T
Cuts at: 6978 6978
Size: 7102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2589 2740 6216 6400 1305
Size: 1284 151 3476 184 2007
Fragments arranged by size:
3476 2007 1284 184 151
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3896 10
Size: 54 1425 343 2064 3216
Fragments arranged by size:
3216 2064 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5060 5419 5578 518
Size: 648 3894 359 159 2042
Fragments arranged by size:
3894 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3416 4518 5195 6343 6588 1606
Size: 1810 1102 677 1148 245 2120
Fragments arranged by size:
2120 1810 1148 1102 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3348 513
Size: 552 2283 4267
Fragments arranged by size:
4267 2283 552
NdeI CA'TA_TG
Cuts at: 387 3478 387
Size: 3091 4011
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7102
NotI GC'GGCC_GC
Cuts at: 4549 4549
Size: 7102
NspI r_CATG'y
Cuts at: 1132 1229 4460 6005 1132
Size: 97 3231 1545 2229
Fragments arranged by size:
3231 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7102
PciI A'CATG_T
Cuts at: 6001 6001
Size: 7102
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7102
PfoI T'CCnGG_A
Cuts at: 2800 2982 4089 2800
Size: 182 1107 5813
Fragments arranged by size:
5813 1107 182
PmeI GTTT'AAAC
Cuts at: 4406 4406
Size: 7102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5171 5203 6684 6716 5171
Size: 32 1481 32 5557
Fragments arranged by size:
5557 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2551
Size: 7102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7102
PsiI TTA'TAA
Cuts at: 4672 4672
Size: 7102
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3364 3396 3364
Size: 32 7070
PstI C_TGCA'G
Cuts at: 830 2262 2834 4454 830
Size: 1432 572 1620 3478
Fragments arranged by size:
3478 1620 1432 572
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7102
PvuII CAG'CTG
Cuts at: 3416 3416
Size: 7102
SacI G_AGCT'C
Cuts at: 3222 4269 3222
Size: 1047 6055
SalI G'TCGA_C
Cuts at: 1936 4444 1936
Size: 2508 4594
SapI GCTCTTCn'nnn_
Cuts at: 4217 4217
Size: 7102
SbfI CC_TGCA'GG
Cuts at: 4454 4454
Size: 7102
ScaI AGT'ACT
Cuts at: 5350 7062 5350
Size: 1712 5390
SfcI C'TryA_G
Cuts at: 826 1045 2258 2830 3488 3662 3785 4051 4121
Size: 219 1213 572 658 174 123 266 70
Cuts at: 4121 4450 5585 6266 6457 826
Size: 329 1135 681 191 1471
Fragments arranged by size:
1471 1213 1135 681 658 572 329 266
219 191 174 123 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7102
SmaI CCC'GGG
Cuts at: 1872 2417 4429 1872
Size: 545 2012 4545
Fragments arranged by size:
4545 2012 545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 2345 2453 3537 5206 6107
Size: 197 926 217 185 108 1084 1669 901
Cuts at: 6107 6369 820
Size: 262 1553
Fragments arranged by size:
1669 1553 1084 926 901 262 217 197
185 108
SnaBI TAC'GTA
Cuts at: 493 4893 493
Size: 4400 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7102
SphI G_CATG'C
Cuts at: 1132 4460 1132
Size: 3328 3774
SspI AAT'ATT
Cuts at: 5 52 5026 5
Size: 47 4974 2081
Fragments arranged by size:
4974 2081 47
StuI AGG'CCT
Cuts at: 1517 3289 1517
Size: 1772 5330
StyI C'CwwG_G
Cuts at: 513 1065 2130 2649 3069 3348 3531 4541 513
Size: 552 1065 519 420 279 183 1010 3074
Fragments arranged by size:
3074 1065 1010 552 519 420 279 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3246 3774 3848 5129 5146 5299 5484
Size: 1255 1666 528 74 1281 17 153 185
Cuts at: 5484 5929 325
Size: 445 1498
Fragments arranged by size:
1666 1498 1281 1255 528 445 185 153
74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2704 4110 5348
Size: 274 80 33 51 1443 727 1406 1238
Cuts at: 5348 7060 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1406 1238 727 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 7062 7094 2753
Size: 32 4277 32 2761
Fragments arranged by size:
4277 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7102
XbaI T'CTAG_A
Cuts at: 4438 4438
Size: 7102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2497
Size: 7102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7102
XmaI C'CCGG_G
Cuts at: 1870 2415 4427 1870
Size: 545 2012 4545
Fragments arranged by size:
4545 2012 545
XmnI GAAnn'nnTTC
Cuts at: 3268 4353 5231 3268
Size: 1085 878 5139
Fragments arranged by size:
5139 1085 878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4910 276
Size: 53 83 186 936 3376 2468
Fragments arranged by size:
3376 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI