Restriction map for
pFN21AA0546
(Circular) (Six-base) MAPSORT of: pFN21AA0546.seq Check: 7886 from: 1 to: 10435
pFN21AA0546 10435 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3169 3169
Size: 10435
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8245 278
Size: 53 83 186 936 6709 2468
Fragments arranged by size:
6709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5930 7778 1937
Size: 3993 1848 4594
Fragments arranged by size:
4594 3993 1848
Acc65I G'GTAC_C
Cuts at: 7756 7756
Size: 10435
AclI AA'CG_TT
Cuts at: 1563 8562 8935 1563
Size: 6999 373 3063
Fragments arranged by size:
6999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3125 3405 3495 3843 4338 4421 4617 4664
Size: 1493 280 90 348 495 83 196 47
Cuts at: 4664 5103 5706 5893 8498 9882 10150 1632
Size: 439 603 187 2605 1384 268 1917
Fragments arranged by size:
2605 1917 1493 1384 603 495 439 348
280 268 196 187 90 83 47
AflII C'TTAA_G
Cuts at: 820 1017 5177 820
Size: 197 4160 6078
Fragments arranged by size:
6078 4160 197
AflIII A'CryG_T
Cuts at: 1203 2277 5582 6726 9334 10311 1203
Size: 1074 3305 1144 2608 977 1327
Fragments arranged by size:
3305 2608 1327 1144 1074 977
AgeI A'CCGG_T
Cuts at: 9266 9266
Size: 10435
AhdI GACnn_n'nnGTC
Cuts at: 5659 9164 5659
Size: 3505 6930
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10435
AlwNI CAG_nnn'CTG
Cuts at: 2251 3518 6919 7466 7672 8178 9750 2251
Size: 1267 3401 547 206 506 1572 2936
Fragments arranged by size:
3401 2936 1572 1267 547 506 206
ApaLI G'TGCA_C
Cuts at: 6492 8492 9648 6492
Size: 2000 1156 7279
Fragments arranged by size:
7279 2000 1156
ApoI r'AATT_y
Cuts at: 1493 2257 3040 3426 4549 4644 4689 4941 5021
Size: 764 783 386 1123 95 45 252 80
Cuts at: 5021 5106 6210 6605 6626 7037 7088 7204 7418
Size: 85 1104 395 21 411 51 116 214
Cuts at: 7418 7424 7517 7744 7970 8122 10231 1493
Size: 6 93 227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1123 1104 783 764 411 395
386 252 227 226 214 152 116 95
93 85 80 51 45 21 6
AseI AT'TA_AT
Cuts at: 160 1032 8989 160
Size: 872 7957 1606
Fragments arranged by size:
7957 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10435
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 4758 7760 1870
Size: 73 244 2571 3002 4545
Fragments arranged by size:
4545 3002 2571 244 73
AvrII C'CTAG_G
Cuts at: 4046 4046
Size: 10435
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5982 6015 5982
Size: 33 10402
BamHI G'GATC_C
Cuts at: 2546 7397 7765 8132 2546
Size: 4851 368 367 4849
Fragments arranged by size:
4851 4849 368 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6216 7756 9211 618
Size: 325 217 627 4429 1540 1455 1842
Fragments arranged by size:
4429 1842 1540 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 2583 2603 5519 7754 1391
Size: 981 211 20 2916 2235 4072
Fragments arranged by size:
4072 2916 2235 981 211 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4076 4409 928
Size: 971 2177 333 6954
Fragments arranged by size:
6954 2177 971 333
BbvCI CC'TCA_GC
Cuts at: 2252 2252
Size: 10435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8626 8660 1903
Size: 34 6689 34 3678
Fragments arranged by size:
6689 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3598 4044 5983 8327 9537 3598
Size: 446 1939 2344 1210 4496
Fragments arranged by size:
4496 2344 1939 1210 446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10435
BfrBI ATG'CAT
Cuts at: 2727 5208 6515 2727
Size: 2481 1307 6647
Fragments arranged by size:
6647 2481 1307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9046 136
Size: 107 122 71 631 605 7374 1525
Fragments arranged by size:
7374 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3433 5138 10430 3433
Size: 1705 5292 3438
Fragments arranged by size:
5292 3438 1705
BlpI GC'TnA_GC
Cuts at: 4239 7852 4239
Size: 3613 6822
Bme1580I G_kGCm'C
Cuts at: 1790 4969 6496 8496 9652 1790
Size: 3179 1527 2000 1156 2573
Fragments arranged by size:
3179 2573 2000 1527 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 8166 9124 442
Size: 416 53 83 399 24 6749 958 1753
Fragments arranged by size:
6749 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2494 3612 3672 3957 4836 4983 5988
Size: 781 474 1118 60 285 879 147 1005
Cuts at: 5988 6282 6869 9095 1239
Size: 294 587 2226 2579
Fragments arranged by size:
2579 2226 1118 1005 879 781 587 474
294 285 147 60
Bpu10I CC'TnA_GC
Cuts at: 2252 5513 2252
Size: 3261 7174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6083 8560 9425 9723 6083
Size: 2477 865 298 6795
Fragments arranged by size:
6795 2477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2343 2625 9098 882
Size: 617 844 282 6473 2219
Fragments arranged by size:
6473 2219 844 617 282
BsaAI yAC'GTr
Cuts at: 493 1204 6361 8226 493
Size: 711 5157 1865 2702
Fragments arranged by size:
5157 2702 1865 711
BsaBI GATnn'nnATC
Cuts at: 10429 10429
Size: 10435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8242 8624 9259 275
Size: 53 83 186 936 6709 382 635 1451
Fragments arranged by size:
6709 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 4303 5545 8868 9266 9540 9687 10098 2334
Size: 1969 1242 3323 398 274 147 411 2671
Fragments arranged by size:
3323 2671 1969 1242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3610 3640 3798 3828 1178
Size: 30 165 30 2207 30 158 30 7785
Fragments arranged by size:
7785 2207 165 158 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2166 2502 2517 2550 2553 2882 3505 3576
Size: 980 336 15 33 3 329 623 71
Cuts at: 3576 3783 3884 5823 1186
Size: 207 101 1939 5798
Fragments arranged by size:
5798 1939 980 623 336 329 207 101
71 33 15 3
BseYI C'CCAG_C
Cuts at: 2306 4420 7566 9638 10134 2306
Size: 2114 3146 2072 496 2607
Fragments arranged by size:
3146 2607 2114 2072 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2539 2795 5709 6284 6449 6821 1163
Size: 573 803 256 2914 575 165 372 4777
Fragments arranged by size:
4777 2914 803 575 573 372 256 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2396 7885 8646 9674 1540
Size: 275 186 12 383 5489 761 1028 2301
Fragments arranged by size:
5489 2301 1028 761 383 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2372 2603 5519 6143 6496 8496 8581 9652 2372
Size: 231 2916 624 353 2000 85 1071 3155
Fragments arranged by size:
3155 2916 2000 1071 624 353 231 85
BsmI GAATG_Cn'
Cuts at: 2729 4873 5210 6517 7015 7570 7946 8039 2729
Size: 2144 337 1307 498 555 376 93 5125
Fragments arranged by size:
5125 2144 1307 555 498 376 337 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2372 2583 2603 4969 5519 6143 6496
Size: 399 582 211 20 2366 550 624 353
Cuts at: 6496 7754 8496 8581 9652 1391
Size: 1258 742 85 1071 2174
Fragments arranged by size:
2366 2174 1258 1071 742 624 582 550
399 353 211 85 20
BspEI T'CCGG_A
Cuts at: 10098 10098
Size: 10435
BspHI T'CATG_A
Cuts at: 3468 5047 8324 10054 3468
Size: 1579 3277 1730 3849
Fragments arranged by size:
3849 3277 1730 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3169 4468 7790 844
Size: 2325 1299 3322 3489
Fragments arranged by size:
3489 3322 2325 1299
BsrBI CCG'CTC
Cuts at: 1394 1823 2287 2410 8322 1394
Size: 429 464 123 5912 3507
Fragments arranged by size:
5912 3507 464 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 4470 6873 7666 8930 9104 65
Size: 1183 3222 2403 793 1264 174 1396
Fragments arranged by size:
3222 2403 1396 1264 1183 793 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9079 9266 1700
Size: 110 7269 187 2869
Fragments arranged by size:
7269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3223 6085 96
Size: 3127 2862 4446
Fragments arranged by size:
4446 3127 2862
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10435
BssSI C'ACGA_G
Cuts at: 8495 9507 8495
Size: 1012 9423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10435
BstBI TT'CG_AA
Cuts at: 9239 9239
Size: 10435
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10435
BstXI CCAn_nnnn'nTGG
Cuts at: 5880 6213 7582 5880
Size: 333 1369 8733
Fragments arranged by size:
8733 1369 333
BstYI r'GATC_y
Cuts at: 1972 2198 2546 2782 3433 3514 3826 5138 7138
Size: 226 348 236 651 81 312 1312 2000
Cuts at: 7138 7397 7765 8132 8517 8534 9986 10072 10084
Size: 259 368 367 385 17 1452 86 12
Cuts at: 10084 10430 1972
Size: 346 1977
Fragments arranged by size:
2000 1977 1452 1312 651 385 368 367
348 346 312 259 236 226 86 81
17 12
Bsu36I CC'TnA_GG
Cuts at: 2297 2865 3524 3720 2297
Size: 568 659 196 9012
Fragments arranged by size:
9012 659 568 196
BtgI C'CryG_G
Cuts at: 513 1065 2004 2162 6689 513
Size: 552 939 158 4527 4259
Fragments arranged by size:
4527 4259 939 552 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6028 487
Size: 683 56 4802 4894
Fragments arranged by size:
4894 4802 683 56
BtsI GCAGTG_nn'
Cuts at: 4776 6759 6981 7498 7951 8745 8765 4776
Size: 1983 222 517 453 794 20 6446
Fragments arranged by size:
6446 1983 794 517 453 222 20
ClaI AT'CG_AT
Cuts at: 1554 3463 6568 1554
Size: 1909 3105 5421
Fragments arranged by size:
5421 3105 1909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4752 4787 529
Size: 35 4188 35 6177
Fragments arranged by size:
6177 4188 35 35
DraI TTT'AAA
Cuts at: 2694 4545 5322 6165 7108 7739 2694
Size: 1851 777 843 943 631 5390
Fragments arranged by size:
5390 1851 943 843 777 631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9442 809
Size: 507 8126 1802
Fragments arranged by size:
8126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2393 3261 4773
Size: 54 1425 50 275 18 563 868 1512
Cuts at: 4773 7719 7882 8771 10400 8
Size: 2946 163 889 1629 43
Fragments arranged by size:
2946 1629 1512 1425 889 868 563 275
163 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2393 7882 1537
Size: 275 581 5489 4090
Fragments arranged by size:
5489 4090 581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2224 2242 2591 2760 3096 3144 3357 3387
Size: 871 18 349 169 336 48 213 30
Cuts at: 3387 3861 4347 4743 5004 5457 7278 8365 1353
Size: 474 486 396 261 453 1821 1087 3423
Fragments arranged by size:
3423 1821 1087 871 486 474 453 396
349 336 261 213 169 48 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2220 2334 9022 9396 9542 387
Size: 1064 138 631 114 6688 374 146 1280
Fragments arranged by size:
6688 1280 1064 631 374 146 138 114
EcoICRI GAG'CTC
Cuts at: 2370 2601 5517 2370
Size: 231 2916 7288
Fragments arranged by size:
7288 2916 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2494 3125 3405 3495 3612 3672
Size: 393 388 474 631 280 90 117 60
Cuts at: 3672 3843 3957 4338 4421 4617 4664 4836 4983
Size: 171 114 381 83 196 47 172 147
Cuts at: 4983 5103 5706 5893 5988 6282 6869 8498 9095
Size: 120 603 187 95 294 587 1629 597
Cuts at: 9095 9882 10150 1239
Size: 787 268 1524
Fragments arranged by size:
1629 1524 787 631 603 597 587 474
393 388 381 294 280 268 196 187
172 171 147 120 117 114 95 90
83 60 47
EcoNI CCTnn'n_nnAGG
Cuts at: 2532 2532
Size: 10435
EcoO109I rG'GnC_Cy
Cuts at: 2303 4076 2303
Size: 1773 8662
EcoRI G'AATT_C
Cuts at: 6605 7418 7744 8122 6605
Size: 813 326 378 8918
Fragments arranged by size:
8918 813 378 326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 2705 3525 3917 5157 2084
Size: 621 820 392 1240 7362
Fragments arranged by size:
7362 1240 820 621 392
FspI TGC'GCA
Cuts at: 8941 8941
Size: 10435
HaeII r_GCGC'y
Cuts at: 1678 9582 1678
Size: 7904 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 3059 3091 3095
Size: 32 135 32 529 32 926 32 4
Cuts at: 3095 3127 3929 3961 4042 4074 4142 4174 4478
Size: 32 802 32 81 32 68 32 304
Cuts at: 4478 4510 5645 5677 5756 5788 5912 5944 7169
Size: 32 1135 32 79 32 124 32 1225
Cuts at: 7169 7201 7351 7383 7490 7522 9076 9108 9150
Size: 32 150 32 107 32 1554 32 42
Cuts at: 9150 9182 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 1225 1135 926 802 529 304
150 135 124 107 81 79 68 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 4182 4657 6667 7779 8025
Size: 988 281 1269 975 475 2010 1112 246
Cuts at: 8025 669
Size: 3079
Fragments arranged by size:
3079 2010 1269 1112 988 975 475 281
246
HindIII A'AGCT_T
Cuts at: 748 4245 4676 4725 6101 6310 6759 748
Size: 3497 431 49 1376 209 449 4424
Fragments arranged by size:
4424 3497 1376 449 431 209 49
HpaI GTT'AAC
Cuts at: 4657 6667 8025 4657
Size: 2010 1358 7067
Fragments arranged by size:
7067 2010 1358
KpnI G_GTAC'C
Cuts at: 7760 7760
Size: 10435
MfeI C'AATT_G
Cuts at: 7173 8034 7173
Size: 861 9574
MluI A'CGCG_T
Cuts at: 2277 10311 2277
Size: 8034 2401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2099 2694 3021 6357 7533 9549 9733 1305
Size: 794 595 327 3336 1176 2016 184 2007
Fragments arranged by size:
3336 2016 2007 1176 794 595 327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4775 7721 10
Size: 54 1425 343 2943 2946 2724
Fragments arranged by size:
2946 2943 2724 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6824 8393 8752 8911 518
Size: 648 5658 1569 359 159 2042
Fragments arranged by size:
5658 2042 1569 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2164 2251 3373 4399 5314 6284 6463
Size: 533 25 87 1122 1026 915 970 179
Cuts at: 6463 7851 8528 9676 9921 1606
Size: 1388 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 1122 1026 970 915 677
533 245 179 87 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 6689 513
Size: 552 939 4685 4259
Fragments arranged by size:
4685 4259 939 552
NdeI CA'TA_TG
Cuts at: 387 7029 7306 387
Size: 6642 277 3516
Fragments arranged by size:
6642 3516 277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10435
NotI GC'GGCC_GC
Cuts at: 7882 7882
Size: 10435
NsiI A_TGCA'T
Cuts at: 2729 5210 6517 2729
Size: 2481 1307 6647
Fragments arranged by size:
6647 2481 1307
NspI r_CATG'y
Cuts at: 1132 1229 5586 6730 7716 7793 9338 1132
Size: 97 4357 1144 986 77 1545 2229
Fragments arranged by size:
4357 2229 1545 1144 986 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10435
PciI A'CATG_T
Cuts at: 5582 6726 9334 5582
Size: 1144 2608 6683
Fragments arranged by size:
6683 2608 1144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6847 6919 1108
Size: 5739 72 4624
Fragments arranged by size:
5739 4624 72
PfoI T'CCnGG_A
Cuts at: 5649 6846 7165 5649
Size: 1197 319 8919
Fragments arranged by size:
8919 1197 319
PmeI GTTT'AAAC
Cuts at: 7739 7739
Size: 10435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8504 8536 10017 10049 8504
Size: 32 1481 32 8890
Fragments arranged by size:
8890 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2303 4076 2303
Size: 1773 8662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10435
PsiI TTA'TAA
Cuts at: 8005 8005
Size: 10435
PstI C_TGCA'G
Cuts at: 830 7787 830
Size: 6957 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10435
PvuII CAG'CTG
Cuts at: 2251 4399 5314 6284 6463 2251
Size: 2148 915 970 179 6223
Fragments arranged by size:
6223 2148 970 915 179
SacI G_AGCT'C
Cuts at: 2372 2603 5519 2372
Size: 231 2916 7288
Fragments arranged by size:
7288 2916 231
SacII CC_GC'GG
Cuts at: 2165 2165
Size: 10435
SalI G'TCGA_C
Cuts at: 1936 7777 1936
Size: 5841 4594
SanDI GG'GwC_CC
Cuts at: 2303 2303
Size: 10435
SapI GCTCTTCn'nnn_
Cuts at: 2224 2591 3144 4743 2224
Size: 367 553 1599 7916
Fragments arranged by size:
7916 1599 553 367
SbfI CC_TGCA'GG
Cuts at: 7787 7787
Size: 10435
ScaI AGT'ACT
Cuts at: 5701 6116 8683 10395 5701
Size: 415 2567 1712 5741
Fragments arranged by size:
5741 2567 1712 415
SfcI C'TryA_G
Cuts at: 826 1045 3279 5232 5373 5470 5931 7783 8918
Size: 219 2234 1953 141 97 461 1852 1135
Cuts at: 8918 9599 9790 826
Size: 681 191 1471
Fragments arranged by size:
2234 1953 1852 1471 1135 681 461 219
191 141 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10435
SmaI CCC'GGG
Cuts at: 1872 2189 7762 1872
Size: 317 5573 4545
Fragments arranged by size:
5573 4545 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5177 6098 8539 9440 9702 820
Size: 197 926 3234 921 2441 901 262 1553
Fragments arranged by size:
3234 2441 1553 926 921 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6361 8226 493
Size: 5868 1865 2702
Fragments arranged by size:
5868 2702 1865
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10435
SphI G_CATG'C
Cuts at: 1132 7793 1132
Size: 6661 3774
SspI AAT'ATT
Cuts at: 5 52 6418 7162 8359 5
Size: 47 6366 744 1197 2081
Fragments arranged by size:
6366 2081 1197 744 47
StuI AGG'CCT
Cuts at: 1517 2173 2452 6803 1517
Size: 656 279 4351 5149
Fragments arranged by size:
5149 4351 656 279
StyI C'CwwG_G
Cuts at: 513 1065 2004 2424 4046 5496 6150 6252 6689
Size: 552 939 420 1622 1450 654 102 437
Cuts at: 6689 7581 7874 513
Size: 892 293 3074
Fragments arranged by size:
3074 1622 1450 939 892 654 552 437
420 293 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 8462 8479 8632 8817 9262 325
Size: 1255 903 5979 17 153 185 445 1498
Fragments arranged by size:
5979 1498 1255 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3223 3245 3456
Size: 274 80 33 51 1443 1246 22 211
Cuts at: 3456 5699 6085 6114 7345 8681 10393 96
Size: 2243 386 29 1231 1336 1712 138
Fragments arranged by size:
2243 1712 1443 1336 1246 1231 386 274
211 138 80 51 33 29 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 10395 10427 3752
Size: 32 6611 32 3760
Fragments arranged by size:
6611 3760 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4080 1316
Size: 2764 7671
XbaI T'CTAG_A
Cuts at: 7771 7771
Size: 10435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7729 7729
Size: 10435
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10435
XmaI C'CCGG_G
Cuts at: 1870 2187 7760 1870
Size: 317 5573 4545
Fragments arranged by size:
5573 4545 317
XmnI GAAnn'nnTTC
Cuts at: 4626 4644 4689 4873 5250 5505 8564 4626
Size: 18 45 184 377 255 3059 6497
Fragments arranged by size:
6497 3059 377 255 184 45 18
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8243 276
Size: 53 83 186 936 6709 2468
Fragments arranged by size:
6709 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SexAI SfiI SfoI SrfI SwaI