Restriction map for
pFN21AA0542
(Circular) (Six-base) MAPSORT of: pFN21AA0542.seq Check: 9297 from: 1 to: 8338
pFN21AA0542 8338 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5445 5503 5445
Size: 58 8280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6148 278
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 5681 1937
Size: 1362 2382 4594
Fragments arranged by size:
4594 2382 1362
Acc65I G'GTAC_C
Cuts at: 5659 5659
Size: 8338
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 6401 7785 8053 1632
Size: 971 3798 1384 268 1917
Fragments arranged by size:
3798 1917 1384 971 268
AfeI AGC'GCT
Cuts at: 4190 4190
Size: 8338
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8141
AflIII A'CryG_T
Cuts at: 1203 3443 7237 8214 1203
Size: 2240 3794 977 1327
Fragments arranged by size:
3794 2240 1327 977
AgeI A'CCGG_T
Cuts at: 7169 7169
Size: 8338
AhdI GACnn_n'nnGTC
Cuts at: 7067 7067
Size: 8338
AleI CACnn'nnGTG
Cuts at: 1166 2973 3268 1166
Size: 1807 295 6236
Fragments arranged by size:
6236 1807 295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3286 4367 1184
Size: 2102 1081 5155
Fragments arranged by size:
5155 2102 1081
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4045 4077 5408 5440 4045
Size: 32 1331 32 6943
Fragments arranged by size:
6943 1331 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2797 3160 3174 3399 3565 3763 3826 3961 4167
Size: 363 14 225 166 198 63 135 206
Cuts at: 4167 4228 4270 4630 4741 5489 5557 5620 5632
Size: 61 42 360 111 748 68 63 12
Cuts at: 5632 6081 7653 2797
Size: 449 1572 3482
Fragments arranged by size:
3482 1572 748 449 363 360 225 206
198 166 135 111 68 63 63 61
42 14 12
ApaI G_GGCC'C
Cuts at: 2752 4444 5101 2752
Size: 1692 657 5989
Fragments arranged by size:
5989 1692 657
ApaLI G'TGCA_C
Cuts at: 6395 7551 6395
Size: 1156 7182
ApoI r'AATT_y
Cuts at: 1493 3743 5647 5873 6025 8134 1493
Size: 2250 1904 226 152 2109 1697
Fragments arranged by size:
2250 2109 1904 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3064 3064
Size: 8338
AseI AT'TA_AT
Cuts at: 160 1032 6892 160
Size: 872 5860 1606
Fragments arranged by size:
5860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 4002 5663 1870
Size: 73 2059 1661 4545
Fragments arranged by size:
4545 2059 1661 73
AvrII C'CTAG_G
Cuts at: 5005 5234 5005
Size: 229 8109
BamHI G'GATC_C
Cuts at: 2175 5668 6035 2175
Size: 3493 367 4478
Fragments arranged by size:
4478 3493 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3408 3588 4577 4820 5594
Size: 325 217 627 1621 180 989 243 774
Cuts at: 5594 5659 7114 618
Size: 65 1455 1842
Fragments arranged by size:
1842 1621 1455 989 774 627 325 243
217 180 65
BanII G_rGCy'C
Cuts at: 1391 2752 3367 3708 4444 4867 4893 4908 5101
Size: 1361 615 341 736 423 26 15 193
Cuts at: 5101 5571 5657 1391
Size: 470 86 4072
Fragments arranged by size:
4072 1361 736 615 470 423 341 193
86 26 15
BbeI G_GCGC'C
Cuts at: 3412 5598 3412
Size: 2186 6152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 2368 2495 4301 928
Size: 971 252 217 127 1806 4965
Fragments arranged by size:
4965 1806 971 252 217 127
BbvCI CC'TCA_GC
Cuts at: 4734 4884 4734
Size: 150 8188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3262 3296 5431 5465 6529 6563 1903
Size: 34 1325 34 2135 34 1064 34 3678
Fragments arranged by size:
3678 2135 1325 1064 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6230 7440 6230
Size: 1210 7128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5318 6949 136
Size: 107 122 71 631 605 3646 1631 1525
Fragments arranged by size:
3646 1631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8333 8333
Size: 8338
BlpI GC'TnA_GC
Cuts at: 2934 5016 5577 5755 2934
Size: 2082 561 178 5517
Fragments arranged by size:
5517 2082 561 178
Bme1580I G_kGCm'C
Cuts at: 1790 2722 2752 3646 3700 4444 5101 6399 7555
Size: 932 30 894 54 744 657 1298 1156
Cuts at: 7555 1790
Size: 2573
Fragments arranged by size:
2573 1298 1156 932 894 744 657 54
30
BmgBI CAC'GTC
Cuts at: 1318 2484 3608 1318
Size: 1166 1124 6048
Fragments arranged by size:
6048 1166 1124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3112 6069 7027
Size: 416 53 83 399 24 1695 2957 958
Cuts at: 7027 442
Size: 1753
Fragments arranged by size:
2957 1753 1695 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3239
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 2744 2945 3508 3706 3796 4256 4295
Size: 1421 84 201 563 198 90 460 39
Cuts at: 4295 4529 4553 4922 5483 5555 6998 1239
Size: 234 24 369 561 72 1443 2579
Fragments arranged by size:
2579 1443 1421 563 561 460 369 234
201 198 90 84 72 39 24
Bpu10I CC'TnA_GC
Cuts at: 2753 4734 4884 2753
Size: 1981 150 6207
Fragments arranged by size:
6207 1981 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3650 4847 5378 5390 6463 7328 7626 3650
Size: 1197 531 12 1073 865 298 4362
Fragments arranged by size:
4362 1197 1073 865 531 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3195 4823 7001 882
Size: 617 1696 1628 2178 2219
Fragments arranged by size:
2219 2178 1696 1628 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 2682 4488 6129 493
Size: 711 1392 86 1806 1641 2702
Fragments arranged by size:
2702 1806 1641 1392 711 86
BsaBI GATnn'nnATC
Cuts at: 8332 8332
Size: 8338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 4942 5595 6145
Size: 53 83 186 936 1876 1533 653 550
Cuts at: 6145 6527 7162 275
Size: 382 635 1451
Fragments arranged by size:
1876 1533 1451 936 653 635 550 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2178 6771 7169 7443 7590 8001 2178
Size: 4593 398 274 147 411 2515
Fragments arranged by size:
4593 2515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 3608 3638 3679
Size: 30 165 30 1593 30 582 30 41
Cuts at: 3679 3709 1178
Size: 30 5807
Fragments arranged by size:
5807 1593 582 165 41 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 4528 4556 4901 5387 5492 5579 1186
Size: 1222 2120 28 345 486 105 87 3945
Fragments arranged by size:
3945 2120 1222 486 345 105 87 28
BseYI C'CCAG_C
Cuts at: 3756 4218 4638 4714 4757 4805 4811 5321 7541
Size: 462 420 76 43 48 6 510 2220
Cuts at: 7541 8037 3756
Size: 496 4057
Fragments arranged by size:
4057 2220 510 496 462 420 76 48
43 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2133 2231 2786 3123 3149 3827 3848
Size: 573 397 98 555 337 26 678 21
Cuts at: 3848 4121 4316 5184 5378 5537 1163
Size: 273 195 868 194 159 3964
Fragments arranged by size:
3964 868 678 573 555 397 337 273
195 194 159 98 26 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5788 6549 7577 1540
Size: 275 186 3787 761 1028 2301
Fragments arranged by size:
3787 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2681 2961 3141 4346 4867 4908 5571 6399 6484
Size: 280 180 1205 521 41 663 828 85
Cuts at: 6484 7555 2681
Size: 1071 3464
Fragments arranged by size:
3464 1205 1071 828 663 521 280 180
85 41
BsmI GAATG_Cn'
Cuts at: 5849 5942 5849
Size: 93 8245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2681 2722 2752 2961 3141 3367 3646
Size: 399 891 41 30 209 180 226 279
Cuts at: 3646 3700 3708 4346 4444 4867 4893 4908 5101
Size: 54 8 638 98 423 26 15 193
Cuts at: 5101 5571 5657 6399 6484 7555 1391
Size: 470 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 891 742 638 470 423 399
279 226 209 193 180 98 86 85
54 41 30 26 15 8
BspEI T'CCGG_A
Cuts at: 2178 8001 2178
Size: 5823 2515
BspHI T'CATG_A
Cuts at: 2545 6227 7957 2545
Size: 3682 1730 2926
Fragments arranged by size:
3682 2926 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2901 3849 4385 4636 5445 5503 5693 844
Size: 2057 948 536 251 809 58 190 3489
Fragments arranged by size:
3489 2057 948 809 536 251 190 58
BsrBI CCG'CTC
Cuts at: 1394 1823 2998 3341 3812 3817 6225 1394
Size: 429 1175 343 471 5 2408 3507
Fragments arranged by size:
3507 2408 1175 471 429 343 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 4797 6833 7007 65
Size: 1183 3549 2036 174 1396
Fragments arranged by size:
3549 2036 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 4260 6982 7169 1700
Size: 110 437 2013 2722 187 2869
Fragments arranged by size:
2869 2722 2013 437 187 110
BsrGI T'GTAC_A
Cuts at: 96 2463 96
Size: 2367 5971
BssHII G'CGCG_C
Cuts at: 1925 3064 1925
Size: 1139 7199
BssSI C'ACGA_G
Cuts at: 2414 6398 7410 2414
Size: 3984 1012 3342
Fragments arranged by size:
3984 3342 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2271 2563 3859 3961 4228 4270 4306 4630
Size: 1477 292 1296 102 267 42 36 324
Cuts at: 4630 5392 5515 794
Size: 762 123 3617
Fragments arranged by size:
3617 1477 1296 762 324 292 267 123
102 42 36
BstBI TT'CG_AA
Cuts at: 2305 2379 7142 2305
Size: 74 4763 3501
Fragments arranged by size:
4763 3501 74
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8338
BstXI CCAn_nnnn'nTGG
Cuts at: 3052 4072 4492 5591 3052
Size: 1020 420 1099 5799
Fragments arranged by size:
5799 1099 1020 420
BstYI r'GATC_y
Cuts at: 1972 2175 5376 5547 5668 6035 6420 6437 7889
Size: 203 3201 171 121 367 385 17 1452
Cuts at: 7889 7975 7987 8333 1972
Size: 86 12 346 1977
Fragments arranged by size:
3201 1977 1452 385 367 346 203 171
121 86 17 12
BstZ17I GTA'TAC
Cuts at: 3300 3300
Size: 8338
Bsu36I CC'TnA_GG
Cuts at: 5490 5490
Size: 8338
BtgI C'CryG_G
Cuts at: 513 1065 3655 3996 513
Size: 552 2590 341 4855
Fragments arranged by size:
4855 2590 552 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3418 4735 487
Size: 683 56 2192 1317 4090
Fragments arranged by size:
4090 2192 1317 683 56
BtsI GCAGTG_nn'
Cuts at: 2841 3263 4251 4316 4541 4911 5854 6648 6668
Size: 422 988 65 225 370 943 794 20
Cuts at: 6668 2841
Size: 4511
Fragments arranged by size:
4511 988 943 794 422 370 225 65
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8303
DraI TTT'AAA
Cuts at: 5642 5642
Size: 8338
DraIII CAC_nnn'GTG
Cuts at: 3859 3859
Size: 8338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7345 809
Size: 507 6029 1802
Fragments arranged by size:
6029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 3042 3537
Size: 54 1425 50 275 18 447 765 495
Cuts at: 3537 5265 5785 6674 8303 8
Size: 1728 520 889 1629 43
Fragments arranged by size:
1728 1629 1425 889 765 520 495 447
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5785 1537
Size: 275 3973 4090
Fragments arranged by size:
4090 3973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2314 2906 3129 3302 4316 6268 1353
Size: 961 592 223 173 1014 1952 3423
Fragments arranged by size:
3423 1952 1014 961 592 223 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4373 4670 6925 7299 7445 387
Size: 1064 138 2784 297 2255 374 146 1280
Fragments arranged by size:
2784 2255 1280 1064 374 297 146 138
EcoICRI GAG'CTC
Cuts at: 4865 4906 5569 4865
Size: 41 663 7634
Fragments arranged by size:
7634 663 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 2660 2744 2945 3508 3706 3796
Size: 393 971 57 84 201 563 198 90
Cuts at: 3796 4256 4295 4529 4553 4922 5483 5555 6401
Size: 460 39 234 24 369 561 72 846
Cuts at: 6401 6998 7785 8053 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 971 846 787 597 563 561 460
393 369 268 234 201 198 90 84
72 57 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3920 4571 5054 5624 3920
Size: 651 483 570 6634
Fragments arranged by size:
6634 651 570 483
EcoO109I rG'GnC_Cy
Cuts at: 2748 2749 4069 4441 4781 5059 5097 5098 5486
Size: 1 1320 372 340 278 38 1 388
Cuts at: 5486 5617 5629 2748
Size: 131 12 5457
Fragments arranged by size:
5457 1320 388 372 340 278 131 38
12 1 1
EcoRI G'AATT_C
Cuts at: 3743 5647 6025 3743
Size: 1904 378 6056
Fragments arranged by size:
6056 1904 378
FspI TGC'GCA
Cuts at: 2264 4968 5453 6844 2264
Size: 2704 485 1391 3758
Fragments arranged by size:
3758 2704 1391 485
HaeII r_GCGC'y
Cuts at: 1678 3412 3875 4192 4544 5112 5584 5598 7485
Size: 1734 463 317 352 568 472 14 1887
Cuts at: 7485 1678
Size: 2531
Fragments arranged by size:
2531 1887 1734 568 472 463 352 317
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 4288 4320 6979
Size: 32 135 32 1048 32 1636 32 2659
Cuts at: 6979 7011 7053 7085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2659 2626 1636 1048 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5682 5928 669
Size: 988 281 3744 246 3079
Fragments arranged by size:
3744 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3672 748
Size: 2924 5414
HpaI GTT'AAC
Cuts at: 5928 5928
Size: 8338
KasI G'GCGC_C
Cuts at: 3408 5594 3408
Size: 2186 6152
KpnI G_GTAC'C
Cuts at: 5663 5663
Size: 8338
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 4938 7452 7636 1305
Size: 867 2766 2514 184 2007
Fragments arranged by size:
2766 2514 2007 867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 3044 3539 5267 10
Size: 54 1425 343 447 765 495 1728 3081
Fragments arranged by size:
3081 1728 1425 765 495 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 3268 6296 6655 6814 518
Size: 648 1807 295 3028 359 159 2042
Fragments arranged by size:
3028 2042 1807 648 359 295 159
MspA1I CmG'CkG
Cuts at: 1606 3157 3325 3958 4234 4267 4402 4426 4630
Size: 1551 168 633 276 33 135 24 204
Cuts at: 4630 4686 5224 5554 5754 6431 7579 7824 1606
Size: 56 538 330 200 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 677 633 538 330 276
245 204 200 168 135 56 33 24
NarI GG'CG_CC
Cuts at: 3409 5595 3409
Size: 2186 6152
NcoI C'CATG_G
Cuts at: 513 1065 3996 513
Size: 552 2931 4855
Fragments arranged by size:
4855 2931 552
NdeI CA'TA_TG
Cuts at: 387 4058 387
Size: 3671 4667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NspI r_CATG'y
Cuts at: 1132 1229 3094 3239 5696 7241 1132
Size: 97 1865 145 2457 1545 2229
Fragments arranged by size:
2457 2229 1865 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8338
PciI A'CATG_T
Cuts at: 7237 7237
Size: 8338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4354 4698 4900 5557 1108
Size: 3246 344 202 657 3889
Fragments arranged by size:
3889 3246 657 344 202
PfoI T'CCnGG_A
Cuts at: 3918 4569 3918
Size: 651 7687
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PmlI CAC'GTG
Cuts at: 2682 4488 2682
Size: 1806 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2526 2558 6407 6439 7920 7952 2526
Size: 32 3849 32 1481 32 2912
Fragments arranged by size:
3849 2912 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4069 4781 4069
Size: 712 7626
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8338
PsiI TTA'TAA
Cuts at: 5908 5908
Size: 8338
PspOMI G'GGCC_C
Cuts at: 2748 4440 5097 2748
Size: 1692 657 5989
Fragments arranged by size:
5989 1692 657
PstI C_TGCA'G
Cuts at: 830 2836 3879 4205 4233 4311 4470 4536 4635
Size: 2006 1043 326 28 78 159 66 99
Cuts at: 4635 5344 5690 830
Size: 709 346 3478
Fragments arranged by size:
3478 2006 1043 709 346 326 159 99
78 66 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8338
PvuII CAG'CTG
Cuts at: 3157 3325 3958 4234 4267 4402 4630 5554 3157
Size: 168 633 276 33 135 228 924 5941
Fragments arranged by size:
5941 924 633 276 228 168 135 33
SacI G_AGCT'C
Cuts at: 4867 4908 5571 4867
Size: 41 663 7634
Fragments arranged by size:
7634 663 41
SalI G'TCGA_C
Cuts at: 1936 5680 1936
Size: 3744 4594
SanDI GG'GwC_CC
Cuts at: 4781 4781
Size: 8338
SapI GCTCTTCn'nnn_
Cuts at: 3129 3129
Size: 8338
SbfI CC_TGCA'GG
Cuts at: 5690 5690
Size: 8338
ScaI AGT'ACT
Cuts at: 4039 6586 8298 4039
Size: 2547 1712 4079
Fragments arranged by size:
4079 2547 1712
SexAI A'CCwGG_T
Cuts at: 4178 4178
Size: 8338
SfcI C'TryA_G
Cuts at: 826 1045 2449 2631 2832 3253 3293 3329 3875
Size: 219 1404 182 201 421 40 36 546
Cuts at: 3875 4201 4229 4307 4466 4532 4631 5340 5686
Size: 326 28 78 159 66 99 709 346
Cuts at: 5686 6821 7502 7693 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1404 1135 709 681 546 421 346
326 219 201 191 182 159 99 78
66 40 36 28
SfoI GGC'GCC
Cuts at: 3410 5596 3410
Size: 2186 6152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8338
SmaI CCC'GGG
Cuts at: 1872 5665 1872
Size: 3793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3665 4826 5357 5369 6442 7343
Size: 197 926 1722 1161 531 12 1073 901
Cuts at: 7343 7605 820
Size: 262 1553
Fragments arranged by size:
1722 1553 1161 1073 926 901 531 262
197 12
SnaBI TAC'GTA
Cuts at: 493 6129 493
Size: 5636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8338
SphI G_CATG'C
Cuts at: 1132 3239 5696 1132
Size: 2107 2457 3774
Fragments arranged by size:
3774 2457 2107
SspI AAT'ATT
Cuts at: 5 52 6262 5
Size: 47 6210 2081
Fragments arranged by size:
6210 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 2879 2990 4550 4616 4981 1517
Size: 1056 306 111 1560 66 365 4874
Fragments arranged by size:
4874 1560 1056 365 306 111 66
StyI C'CwwG_G
Cuts at: 513 1065 3700 3996 4065 5005 5234 5777 513
Size: 552 2635 296 69 940 229 543 3074
Fragments arranged by size:
3074 2635 940 552 543 296 229 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3874 4361 4771 6365 6382 6535 6720
Size: 1255 2294 487 410 1594 17 153 185
Cuts at: 6720 7165 325
Size: 445 1498
Fragments arranged by size:
2294 1594 1498 1255 487 445 410 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2463 2526 4037
Size: 274 80 33 51 1443 486 63 1511
Cuts at: 4037 4501 6584 8296 96
Size: 464 2083 1712 138
Fragments arranged by size:
2083 1712 1511 1443 486 464 274 138
80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2413 2445 3608 3640 3679 3711 8298 8330 2413
Size: 32 1163 32 39 32 4587 32 2421
Fragments arranged by size:
4587 2421 1163 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2143 1316
Size: 827 7511
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4003 4003
Size: 8338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8338
XmaI C'CCGG_G
Cuts at: 1870 5663 1870
Size: 3793 4545
XmnI GAAnn'nnTTC
Cuts at: 2375 3674 6467 2375
Size: 1299 2793 4246
Fragments arranged by size:
4246 2793 1299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6146 276
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
BaeI BfrBI BsiWI BsmBI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SfiI SrfI SwaI