Restriction map for
pFN21AA0536
(Circular) (Six-base) MAPSORT of: pFN21AA0536.seq Check: 1834 from: 1 to: 7726
pFN21AA0536 7726 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5536 278
Size: 53 83 186 936 4000 2468
Fragments arranged by size:
4000 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3271 3478 5069 1937
Size: 1334 207 1591 4594
Fragments arranged by size:
4594 1591 1334 207
Acc65I G'GTAC_C
Cuts at: 5047 5047
Size: 7726
AclI AA'CG_TT
Cuts at: 1563 4060 5853 6226 1563
Size: 2497 1793 373 3063
Fragments arranged by size:
3063 2497 1793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 3552 3693 4249 4718 4923 5789 7173
Size: 825 1095 141 556 469 205 866 1384
Cuts at: 7173 7441 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1095 866 825 556 469 268
205 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7529
AflIII A'CryG_T
Cuts at: 1203 3684 4522 6625 7602 1203
Size: 2481 838 2103 977 1327
Fragments arranged by size:
2481 2103 1327 977 838
AgeI A'CCGG_T
Cuts at: 6557 6557
Size: 7726
AhdI GACnn_n'nnGTC
Cuts at: 2920 6455 2920
Size: 3535 4191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7726
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 3343
Size: 32 7694
AlwNI CAG_nnn'CTG
Cuts at: 3911 4954 5469 7041 3911
Size: 1043 515 1572 4596
Fragments arranged by size:
4596 1572 1043 515
ApaLI G'TGCA_C
Cuts at: 5783 6939 5783
Size: 1156 6570
ApoI r'AATT_y
Cuts at: 1493 2451 3512 4244 4662 5035 5261 5413 7522
Size: 958 1061 732 418 373 226 152 2109
Cuts at: 7522 1493
Size: 1697
Fragments arranged by size:
2109 1697 1061 958 732 418 373 226
152
AseI AT'TA_AT
Cuts at: 160 1032 4851 6280 160
Size: 872 3819 1429 1606
Fragments arranged by size:
3819 1606 1429 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7726
AvaI C'yCGr_G
Cuts at: 1870 1943 3252 5051 1870
Size: 73 1309 1799 4545
Fragments arranged by size:
4545 1799 1309 73
BamHI G'GATC_C
Cuts at: 5056 5423 5056
Size: 367 7359
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3889 4887 5047 6502 618
Size: 325 217 627 2102 998 160 1455 1842
Fragments arranged by size:
2102 1842 1455 998 627 325 217 160
BanII G_rGCy'C
Cuts at: 1391 2409 4176 5045 1391
Size: 1018 1767 869 4072
Fragments arranged by size:
4072 1767 1018 869
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 2199 2331 3043 3585 3941 4355
Size: 971 210 90 132 712 542 356 414
Cuts at: 4355 4911 928
Size: 556 3743
Fragments arranged by size:
3743 971 712 556 542 414 356 210
132 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5917 5951 1903
Size: 34 3980 34 3678
Fragments arranged by size:
3980 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5618 6828 5618
Size: 1210 6516
BclI T'GATC_A
Cuts at: 1548 4774 1548
Size: 3226 4500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6337 136
Size: 107 122 71 631 605 4665 1525
Fragments arranged by size:
4665 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2492 2771 3128 3335 3401 7721 2492
Size: 279 357 207 66 4320 2497
Fragments arranged by size:
4320 2497 357 279 207 66
BlpI GC'TnA_GC
Cuts at: 5143 5143
Size: 7726
Bme1580I G_kGCm'C
Cuts at: 1790 5787 6943 1790
Size: 3997 1156 2573
Fragments arranged by size:
3997 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3275 1318
Size: 1957 5769
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2017 2882 4092
Size: 416 53 83 399 24 600 865 1210
Cuts at: 4092 5457 6415 442
Size: 1365 958 1753
Fragments arranged by size:
1753 1365 1210 958 865 600 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7726
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 7726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 3729 3779 6386 1239
Size: 1029 1461 50 2607 2579
Fragments arranged by size:
2607 2579 1461 1029 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2685 2733 5851 6716 7014 2685
Size: 48 3118 865 298 3397
Fragments arranged by size:
3397 3118 865 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 3111 3318 3360 3387 3462 6389
Size: 617 513 1099 207 42 27 75 2927
Cuts at: 6389 882
Size: 2219
Fragments arranged by size:
2927 2219 1099 617 513 207 75 42
27
BsaAI yAC'GTr
Cuts at: 493 1204 3083 5517 493
Size: 711 1879 2434 2702
Fragments arranged by size:
2702 2434 1879 711
BsaBI GATnn'nnATC
Cuts at: 3739 4230 4779 7720 3739
Size: 491 549 2941 3745
Fragments arranged by size:
3745 2941 549 491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5533 5915 6550 275
Size: 53 83 186 936 4000 382 635 1451
Fragments arranged by size:
4000 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6159 6557 6831 6978 7389 6159
Size: 398 274 147 411 6496
Fragments arranged by size:
6496 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 3148 3178 3343
Size: 30 165 30 606 30 1109 30 165
Cuts at: 3343 3373 1178
Size: 30 5531
Fragments arranged by size:
5531 1109 606 165 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3235 3396 3445 1186
Size: 2049 161 49 5467
Fragments arranged by size:
5467 2049 161 49
BseYI C'CCAG_C
Cuts at: 3029 4966 6929 7425 3029
Size: 1937 1963 496 3330
Fragments arranged by size:
3330 1963 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 5176 5937 6965 1540
Size: 275 186 865 2310 761 1028 2301
Fragments arranged by size:
2310 2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3946 4176 4576 4847 5787 5872 6943 3946
Size: 230 400 271 940 85 1071 4729
Fragments arranged by size:
4729 1071 940 400 271 230 85
BsmI GAATG_Cn'
Cuts at: 5237 5330 5237
Size: 93 7633
BsmBI CGTCTCn'nnnn_
Cuts at: 3217 3217
Size: 7726
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 3946 4176 4576 4847 5045 5787
Size: 399 619 1537 230 400 271 198 742
Cuts at: 5787 5872 6943 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1537 1071 742 619 400 399 271
230 198 85
BspEI T'CCGG_A
Cuts at: 7389 7389
Size: 7726
BspHI T'CATG_A
Cuts at: 2455 5615 7345 2455
Size: 3160 1730 2836
Fragments arranged by size:
3160 2836 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3093 3369 5081 844
Size: 2249 276 1712 3489
Fragments arranged by size:
3489 2249 1712 276
BsrBI CCG'CTC
Cuts at: 1394 1823 5613 1394
Size: 429 3790 3507
Fragments arranged by size:
3790 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4713 6221 6395 65
Size: 1183 3465 1508 174 1396
Fragments arranged by size:
3465 1508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6370 6557 1700
Size: 110 4560 187 2869
Fragments arranged by size:
4560 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4794 96
Size: 4698 3028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7726
BssSI C'ACGA_G
Cuts at: 5786 6798 5786
Size: 1012 6714
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7726
BstBI TT'CG_AA
Cuts at: 4744 6530 4744
Size: 1786 5940
BstEII G'GTnAC_C
Cuts at: 1184 3024 1184
Size: 1840 5886
BstYI r'GATC_y
Cuts at: 1972 2492 2564 2771 2882 3128 3335 3401 4382
Size: 520 72 207 111 246 207 66 981
Cuts at: 4382 4711 5056 5423 5808 5825 7277 7363 7375
Size: 329 345 367 385 17 1452 86 12
Cuts at: 7375 7721 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 520 385 367 346 345
329 246 207 207 111 86 72 66
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 6226
Fragments arranged by size:
6226 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6987
Fragments arranged by size:
6987 683 56
BtsI GCAGTG_nn'
Cuts at: 5242 6036 6056 5242
Size: 794 20 6912
Fragments arranged by size:
6912 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7726
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7691
DraI TTT'AAA
Cuts at: 3517 4194 4494 5030 3517
Size: 677 300 536 6213
Fragments arranged by size:
6213 677 536 300
DraIII CAC_nnn'GTG
Cuts at: 3470 3470
Size: 7726
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6733 809
Size: 507 5417 1802
Fragments arranged by size:
5417 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 5173 6062
Size: 54 1425 50 275 18 177 3166 889
Cuts at: 6062 7691 8
Size: 1629 43
Fragments arranged by size:
3166 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5173 1537
Size: 275 3361 4090
Fragments arranged by size:
4090 3361 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 3169 4268 5656 1353
Size: 1083 733 1099 1388 3423
Fragments arranged by size:
3423 1388 1099 1083 733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6313 6687 6833 387
Size: 1064 138 4724 374 146 1280
Fragments arranged by size:
4724 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4174 4174
Size: 7726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2457 3552 3693 3729 3779 4249
Size: 393 636 189 1095 141 36 50 470
Cuts at: 4249 4718 4923 5789 6386 7173 7441 1239
Size: 469 205 866 597 787 268 1524
Fragments arranged by size:
1524 1095 866 787 636 597 470 469
393 268 205 189 141 50 36
EcoO109I rG'GnC_Cy
Cuts at: 4864 4993 4864
Size: 129 7597
EcoRI G'AATT_C
Cuts at: 5035 5413 5035
Size: 378 7348
EcoRV GAT'ATC
Cuts at: 3262 4450 3262
Size: 1188 6538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3542 3542
Size: 7726
FspI TGC'GCA
Cuts at: 6232 6232
Size: 7726
HaeII r_GCGC'y
Cuts at: 1678 6873 1678
Size: 5195 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2264 2296 2906
Size: 32 135 32 437 32 223 32 610
Cuts at: 2906 2927 2938 2959 2990 3022 3113 3145 3148
Size: 21 11 21 31 32 91 32 3
Cuts at: 3148 3180 3246 3278 3539 3571 4419 4451 4496
Size: 32 66 32 261 32 848 32 45
Cuts at: 4496 4528 4607 4639 6367 6399 6441 6473 1373
Size: 32 79 32 1728 32 42 32 2626
Fragments arranged by size:
2626 1728 848 610 437 261 223 135
91 79 66 45 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 31 21 21 11 3
HincII GTy'rAC
Cuts at: 669 1657 1938 5070 5316 669
Size: 988 281 3132 246 3079
Fragments arranged by size:
3132 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4496 4700 748
Size: 3748 204 3774
Fragments arranged by size:
3774 3748 204
HpaI GTT'AAC
Cuts at: 5316 5316
Size: 7726
KpnI G_GTAC'C
Cuts at: 5051 5051
Size: 7726
MfeI C'AATT_G
Cuts at: 3645 5325 3645
Size: 1680 6046
MluI A'CGCG_T
Cuts at: 7602 7602
Size: 7726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3341 3407 4880 4923 4941 6840 7024 1305
Size: 2036 66 1473 43 18 1899 184 2007
Fragments arranged by size:
2036 2007 1899 1473 184 66 43 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5904
Fragments arranged by size:
5904 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 5684 6043 6202 518
Size: 648 2523 1995 359 159 2042
Fragments arranged by size:
2523 2042 1995 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 3959 5142 5819 6967 7212 1606
Size: 409 1944 1183 677 1148 245 2120
Fragments arranged by size:
2120 1944 1183 1148 677 409 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6235
Fragments arranged by size:
6235 939 552
NdeI CA'TA_TG
Cuts at: 387 4692 387
Size: 4305 3421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7726
NotI GC'GGCC_GC
Cuts at: 5173 5173
Size: 7726
NruI TCG'CGA
Cuts at: 2862 2862
Size: 7726
NspI r_CATG'y
Cuts at: 1132 1229 3688 4445 4526 5084 6629 1132
Size: 97 2459 757 81 558 1545 2229
Fragments arranged by size:
2459 2229 1545 757 558 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7726
PciI A'CATG_T
Cuts at: 3684 4522 6625 3684
Size: 838 2103 4785
Fragments arranged by size:
4785 2103 838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4144 1108
Size: 3036 4690
PfoI T'CCnGG_A
Cuts at: 5013 5013
Size: 7726
PmeI GTTT'AAAC
Cuts at: 5030 5030
Size: 7726
PmlI CAC'GTG
Cuts at: 3083 3083
Size: 7726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3267 3343 3375 5795 5827 7308 7340 3235
Size: 32 76 32 2420 32 1481 32 3621
Fragments arranged by size:
3621 2420 1481 76 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4864 4864
Size: 7726
PshAI GACnn'nnGTC
Cuts at: 1613 2587 3423 1613
Size: 974 836 5916
Fragments arranged by size:
5916 974 836
PsiI TTA'TAA
Cuts at: 2843 5296 2843
Size: 2453 5273
PstI C_TGCA'G
Cuts at: 830 5078 830
Size: 4248 3478
PvuI CG_AT'CG
Cuts at: 2001 2866 2001
Size: 865 6861
SacI G_AGCT'C
Cuts at: 4176 4176
Size: 7726
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 7726
SalI G'TCGA_C
Cuts at: 1936 5068 1936
Size: 3132 4594
SapI GCTCTTCn'nnn_
Cuts at: 3169 3169
Size: 7726
SbfI CC_TGCA'GG
Cuts at: 5078 5078
Size: 7726
ScaI AGT'ACT
Cuts at: 2488 5974 7686 2488
Size: 3486 1712 2528
Fragments arranged by size:
3486 2528 1712
SexAI A'CCwGG_T
Cuts at: 3040 3040
Size: 7726
SfcI C'TryA_G
Cuts at: 826 1045 4387 4626 4997 5074 6209 6890 7081
Size: 219 3342 239 371 77 1135 681 191
Cuts at: 7081 826
Size: 1471
Fragments arranged by size:
3342 1471 1135 681 371 239 219 191
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7726
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7726
SmaI CCC'GGG
Cuts at: 1872 5053 1872
Size: 3181 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2700 2748 3252 5830 6731 6993
Size: 197 926 757 48 504 2578 901 262
Cuts at: 6993 820
Size: 1553
Fragments arranged by size:
2578 1553 926 901 757 504 262 197
48
SnaBI TAC'GTA
Cuts at: 493 5517 493
Size: 5024 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7726
SphI G_CATG'C
Cuts at: 1132 5084 1132
Size: 3952 3774
SspI AAT'ATT
Cuts at: 5 52 5650 5
Size: 47 5598 2081
Fragments arranged by size:
5598 2081 47
StuI AGG'CCT
Cuts at: 1517 3818 1517
Size: 2301 5425
StyI C'CwwG_G
Cuts at: 513 1065 2004 5165 513
Size: 552 939 3161 3074
Fragments arranged by size:
3161 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3043 4025 4875 5753 5770 5923 6108
Size: 1255 1463 982 850 878 17 153 185
Cuts at: 6108 6553 325
Size: 445 1498
Fragments arranged by size:
1498 1463 1255 982 878 850 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2486 2501 4794
Size: 274 80 33 51 1443 509 15 2293
Cuts at: 4794 4921 5972 7684 96
Size: 127 1051 1712 138
Fragments arranged by size:
2293 1712 1443 1051 509 274 138 127
80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4837 4869 7686 7718 4837
Size: 32 2817 32 4845
Fragments arranged by size:
4845 2817 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2023 3042 3160 1316
Size: 707 1019 118 5882
Fragments arranged by size:
5882 1019 707 118
XbaI T'CTAG_A
Cuts at: 5062 5062
Size: 7726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4141 4141
Size: 7726
XhoI C'TCGA_G
Cuts at: 1943 3252 1943
Size: 1309 6417
XmaI C'CCGG_G
Cuts at: 1870 5051 1870
Size: 3181 4545
XmnI GAAnn'nnTTC
Cuts at: 3526 5855 3526
Size: 2329 5397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5534 276
Size: 53 83 186 936 4000 2468
Fragments arranged by size:
4000 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PspOMI
PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI
SwaI