Restriction map for
pFN21AA0531
(Circular) (Six-base) MAPSORT of: pFN21AA0531.seq Check: 6170 from: 1 to: 7576
pFN21AA0531 7576 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5386 278
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4919 1937
Size: 2982 4594
Acc65I G'GTAC_C
Cuts at: 4897 4897
Size: 7576
AclI AA'CG_TT
Cuts at: 1563 5703 6076 1563
Size: 4140 373 3063
Fragments arranged by size:
4140 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 3359 4113 4673 5639 7023 7291 1632
Size: 1220 507 754 560 966 1384 268 1917
Fragments arranged by size:
1917 1384 1220 966 754 560 507 268
AfeI AGC'GCT
Cuts at: 4691 4691
Size: 7576
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7379
AflIII A'CryG_T
Cuts at: 1203 2578 2830 3416 6475 7452 1203
Size: 1375 252 586 3059 977 1327
Fragments arranged by size:
3059 1375 1327 977 586 252
AgeI A'CCGG_T
Cuts at: 6407 6407
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 6305 6305
Size: 7576
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7576
AlwNI CAG_nnn'CTG
Cuts at: 2999 3091 3475 4228 4396 4429 5319 6891 2999
Size: 92 384 753 168 33 890 1572 3684
Fragments arranged by size:
3684 1572 890 753 384 168 92 33
ApaI G_GGCC'C
Cuts at: 4756 4756
Size: 7576
ApaLI G'TGCA_C
Cuts at: 2509 5633 6789 2509
Size: 3124 1156 3296
Fragments arranged by size:
3296 3124 1156
ApoI r'AATT_y
Cuts at: 1493 2087 2099 3658 4503 4855 4885 5111 5263
Size: 594 12 1559 845 352 30 226 152
Cuts at: 5263 7372 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1559 845 594 352 226 152
30 12
AseI AT'TA_AT
Cuts at: 160 1032 6130 160
Size: 872 5098 1606
Fragments arranged by size:
5098 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
AvaI C'yCGr_G
Cuts at: 1870 1943 3531 3827 4437 4901 1870
Size: 73 1588 296 610 464 4545
Fragments arranged by size:
4545 1588 610 464 296 73
BamHI G'GATC_C
Cuts at: 2011 4906 5273 2011
Size: 2895 367 4314
Fragments arranged by size:
4314 2895 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2732 3701 4897 6352 618
Size: 325 217 627 945 969 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 969 945 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2534 4024 4756 4895 1391
Size: 1143 1490 732 139 4072
Fragments arranged by size:
4072 1490 1143 732 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2140 2920 4356 928
Size: 971 241 780 1436 4148
Fragments arranged by size:
4148 1436 971 780 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 4388 4422 5767 5801 1903
Size: 34 1871 34 546 34 1345 34 3678
Fragments arranged by size:
3678 1871 1345 546 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2461 5468 6678 2461
Size: 3007 1210 3359
Fragments arranged by size:
3359 3007 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7576
BfrBI ATG'CAT
Cuts at: 2198 2198
Size: 7576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4730 4751 6187
Size: 107 122 71 631 605 3058 21 1436
Cuts at: 6187 136
Size: 1525
Fragments arranged by size:
3058 1525 1436 631 605 122 107 71
21
BglII A'GATC_T
Cuts at: 2415 3264 3673 7571 2415
Size: 849 409 3898 2420
Fragments arranged by size:
3898 2420 849 409
BlpI GC'TnA_GC
Cuts at: 3602 3620 3995 4993 3602
Size: 18 375 998 6185
Fragments arranged by size:
6185 998 375 18
Bme1580I G_kGCm'C
Cuts at: 1790 2513 4756 5637 6793 1790
Size: 723 2243 881 1156 2573
Fragments arranged by size:
2573 2243 1156 881 723
BmgBI CAC'GTC
Cuts at: 1318 3419 1318
Size: 2101 5475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5307 6265 442
Size: 416 53 83 399 24 3890 958 1753
Fragments arranged by size:
3890 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 4370 3283
Size: 1087 6489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 3113 3194 3253 3407 3452 3497 4133
Size: 1166 708 81 59 154 45 45 636
Cuts at: 4133 4385 4547 4619 4661 6236 1239
Size: 252 162 72 42 1575 2579
Fragments arranged by size:
2579 1575 1166 708 636 252 162 154
81 72 59 45 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5701 6566 6864 5701
Size: 865 298 6413
Fragments arranged by size:
6413 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2936 6239 882
Size: 617 609 828 3303 2219
Fragments arranged by size:
3303 2219 828 617 609
BsaAI yAC'GTr
Cuts at: 493 1204 2581 5367 493
Size: 711 1377 2786 2702
Fragments arranged by size:
2786 2702 1377 711
BsaBI GATnn'nnATC
Cuts at: 4218 7570 4218
Size: 3352 4224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5383 5765 6400 275
Size: 53 83 186 936 3850 382 635 1451
Fragments arranged by size:
3850 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6009 6407 6681 6828 7239 6009
Size: 398 274 147 411 6346
Fragments arranged by size:
6346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3114 3144 4361 4391 1178
Size: 30 165 30 1711 30 1217 30 4363
Fragments arranged by size:
4363 1711 1217 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3254 3275 3422 3494 3761 3764 3979 4196
Size: 2068 21 147 72 267 3 215 217
Cuts at: 4196 4382 4854 1186
Size: 186 472 3908
Fragments arranged by size:
3908 2068 472 267 217 215 186 147
72 21 3
BseYI C'CCAG_C
Cuts at: 2313 2705 3906 6779 7275 2313
Size: 392 1201 2873 496 2614
Fragments arranged by size:
2873 2614 1201 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4382 1163
Size: 573 2646 4357
Fragments arranged by size:
4357 2646 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4818 5026 5787 6815 1540
Size: 275 186 2817 208 761 1028 2301
Fragments arranged by size:
2817 2301 1028 761 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 2513 5637 5722 6793 2513
Size: 3124 85 1071 3296
Fragments arranged by size:
3296 3124 1071 85
BsmI GAATG_Cn'
Cuts at: 2027 3868 5087 5180 2027
Size: 1841 1219 93 4423
Fragments arranged by size:
4423 1841 1219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3425 4164 3425
Size: 739 6837
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2534 4024 4756 4895 5637 5722
Size: 399 723 21 1490 732 139 742 85
Cuts at: 5722 6793 1391
Size: 1071 2174
Fragments arranged by size:
2174 1490 1071 742 732 723 399 139
85 21
BspEI T'CCGG_A
Cuts at: 7239 7239
Size: 7576
BspHI T'CATG_A
Cuts at: 2473 5465 7195 2473
Size: 2992 1730 2854
Fragments arranged by size:
2992 2854 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2176 4584 4931 844
Size: 1332 2408 347 3489
Fragments arranged by size:
3489 2408 1332 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2518 3874 5463 1394
Size: 429 695 1356 1589 3507
Fragments arranged by size:
3507 1589 1356 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6071 6245 65
Size: 1183 4823 174 1396
Fragments arranged by size:
4823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 6220 6407 1700
Size: 110 233 4177 187 2869
Fragments arranged by size:
4177 2869 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 3771 96
Size: 3675 3901
BssHII G'CGCG_C
Cuts at: 1925 4647 1925
Size: 2722 4854
BssSI C'ACGA_G
Cuts at: 5636 6648 5636
Size: 1012 6564
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4396 794
Size: 3602 3974
BstBI TT'CG_AA
Cuts at: 6380 6380
Size: 7576
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7576
BstXI CCAn_nnnn'nTGG
Cuts at: 4741 4741
Size: 7576
BstYI r'GATC_y
Cuts at: 1972 2011 2070 2415 3264 3651 3673 4426 4906
Size: 39 59 345 849 387 22 753 480
Cuts at: 4906 5273 5658 5675 7127 7213 7225 7571 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 849 753 480 387 385 367
346 345 86 59 39 22 17 12
Bsu36I CC'TnA_GG
Cuts at: 2535 3135 2535
Size: 600 6976
BtgI C'CryG_G
Cuts at: 513 1065 2004 2077 2118 2289 2370 3759 4617
Size: 552 939 73 41 171 81 1389 858
Cuts at: 4617 513
Size: 3472
Fragments arranged by size:
3472 1389 939 858 552 171 81 73
41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2124 487
Size: 683 56 898 5939
Fragments arranged by size:
5939 898 683 56
BtsI GCAGTG_nn'
Cuts at: 4394 4489 5092 5886 5906 4394
Size: 95 603 794 20 6064
Fragments arranged by size:
6064 794 603 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7541
DraI TTT'AAA
Cuts at: 2104 4880 2104
Size: 2776 4800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6583 809
Size: 507 5267 1802
Fragments arranged by size:
5267 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4743 4815 5023
Size: 54 1425 50 275 18 2913 72 208
Cuts at: 5023 5912 7541 8
Size: 889 1629 43
Fragments arranged by size:
2913 1629 1425 889 275 208 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4815 5023 1537
Size: 275 3003 208 4090
Fragments arranged by size:
4090 3003 275 208
EarI CTCTTCn'nnn_
Cuts at: 1353 3379 3956 3972 3996 4855 5506 1353
Size: 2026 577 16 24 859 651 3423
Fragments arranged by size:
3423 2026 859 651 577 24 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4637 4772 6163 6537 6683
Size: 1064 138 435 2613 135 1391 374 146
Cuts at: 6683 387
Size: 1280
Fragments arranged by size:
2613 1391 1280 1064 435 374 146 138
135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2852 3113 3194 3253 3359 3407
Size: 393 773 447 261 81 59 106 48
Cuts at: 3407 3452 3497 4113 4133 4385 4547 4619 4661
Size: 45 45 616 20 252 162 72 42
Cuts at: 4661 4673 5639 6236 7023 7291 1239
Size: 12 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 773 616 597 447 393
268 261 252 162 106 81 72 59
48 45 45 42 20 12
EcoO109I rG'GnC_Cy
Cuts at: 3472 4752 3472
Size: 1280 6296
EcoRI G'AATT_C
Cuts at: 4885 5263 4885
Size: 378 7198
EcoRV GAT'ATC
Cuts at: 2350 2350
Size: 7576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2243 2479 2243
Size: 236 7340
FspI TGC'GCA
Cuts at: 6082 6082
Size: 7576
HaeII r_GCGC'y
Cuts at: 1678 3837 4112 4693 6723 1678
Size: 2159 275 581 2030 2531
Fragments arranged by size:
2531 2159 2030 581 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2933 2965 3256 3288 3340
Size: 32 135 32 1361 32 291 32 52
Cuts at: 3340 3372 3491 3523 3785 3817 3913 3945 4361
Size: 32 119 32 262 32 96 32 416
Cuts at: 4361 4393 6217 6249 6291 6323 1373
Size: 32 1824 32 42 32 2626
Fragments arranged by size:
2626 1824 1361 416 291 262 135 119
96 52 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4920 5166 669
Size: 988 281 2982 246 3079
Fragments arranged by size:
3079 2982 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4247 748
Size: 3499 4077
HpaI GTT'AAC
Cuts at: 5166 5166
Size: 7576
KpnI G_GTAC'C
Cuts at: 4901 4901
Size: 7576
MfeI C'AATT_G
Cuts at: 5175 5175
Size: 7576
MluI A'CGCG_T
Cuts at: 7452 7452
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3333 3873 4440 4835 6690 6874 1305
Size: 2028 540 567 395 1855 184 2007
Fragments arranged by size:
2028 2007 1855 567 540 395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4745 10
Size: 54 1425 343 2913 2841
Fragments arranged by size:
2913 2841 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2957 5534 5893 6052 518
Size: 648 867 924 2577 359 159 2042
Fragments arranged by size:
2577 2042 924 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 3325 3547 3895 4156 4555 4992 5669
Size: 473 1246 222 348 261 399 437 677
Cuts at: 5669 6817 7062 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1246 1148 677 473 437 399 348
261 245 222
NcoI C'CATG_G
Cuts at: 513 1065 2004 2289 2370 3759 513
Size: 552 939 285 81 1389 4330
Fragments arranged by size:
4330 1389 939 552 285 81
NdeI CA'TA_TG
Cuts at: 387 2143 387
Size: 1756 5820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7576
NotI GC'GGCC_GC
Cuts at: 5023 5023
Size: 7576
NsiI A_TGCA'T
Cuts at: 2200 2200
Size: 7576
NspI r_CATG'y
Cuts at: 1132 1229 2202 2834 3946 4934 6479 1132
Size: 97 973 632 1112 988 1545 2229
Fragments arranged by size:
2229 1545 1112 988 973 632 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7576
PciI A'CATG_T
Cuts at: 2830 6475 2830
Size: 3645 3931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 2320 3091 3475 1108
Size: 1182 30 771 384 5209
Fragments arranged by size:
5209 1182 771 384 30
PfoI T'CCnGG_A
Cuts at: 4050 4422 4560 4050
Size: 372 138 7066
Fragments arranged by size:
7066 372 138
PmeI GTTT'AAAC
Cuts at: 4880 4880
Size: 7576
PmlI CAC'GTG
Cuts at: 2581 2581
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 5677 7158 7190 5645
Size: 32 1481 32 6031
Fragments arranged by size:
6031 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7576
PsiI TTA'TAA
Cuts at: 2238 4263 5146 2238
Size: 2025 883 4668
Fragments arranged by size:
4668 2025 883
PspOMI G'GGCC_C
Cuts at: 4752 4752
Size: 7576
PstI C_TGCA'G
Cuts at: 830 2894 4928 830
Size: 2064 2034 3478
Fragments arranged by size:
3478 2064 2034
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7576
PvuII CAG'CTG
Cuts at: 3325 3547 3895 4156 4555 3325
Size: 222 348 261 399 6346
Fragments arranged by size:
6346 399 348 261 222
SacII CC_GC'GG
Cuts at: 2080 2080
Size: 7576
SalI G'TCGA_C
Cuts at: 1936 4918 1936
Size: 2982 4594
SapI GCTCTTCn'nnn_
Cuts at: 3996 4855 3996
Size: 859 6717
SbfI CC_TGCA'GG
Cuts at: 4928 4928
Size: 7576
ScaI AGT'ACT
Cuts at: 5824 7536 5824
Size: 1712 5864
SfcI C'TryA_G
Cuts at: 826 1045 2890 3234 3277 4924 6059 6740 6931
Size: 219 1845 344 43 1647 1135 681 191
Cuts at: 6931 826
Size: 1471
Fragments arranged by size:
1845 1647 1471 1135 681 344 219 191
43
SfiI GGCCn_nnn'nGGCC
Cuts at: 4730 4751 4730
Size: 21 7555
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7576
SmaI CCC'GGG
Cuts at: 1872 3533 4903 1872
Size: 1661 1370 4545
Fragments arranged by size:
4545 1661 1370
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3827 5680 6581 6843 820
Size: 197 926 1884 1853 901 262 1553
Fragments arranged by size:
1884 1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5367 493
Size: 4874 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7576
SphI G_CATG'C
Cuts at: 1132 4934 1132
Size: 3802 3774
SspI AAT'ATT
Cuts at: 5 52 2641 3208 5500 5
Size: 47 2589 567 2292 2081
Fragments arranged by size:
2589 2292 2081 567 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7576
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2370 3156 3444 3759 4662
Size: 552 939 285 81 786 288 315 903
Cuts at: 4662 5015 513
Size: 353 3074
Fragments arranged by size:
3074 939 903 786 552 353 315 288
285 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2870 5603 5620 5773 5958 6403 325
Size: 1255 1290 2733 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1290 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3771 5822 7534
Size: 274 80 33 51 1443 1794 2051 1712
Cuts at: 7534 96
Size: 138
Fragments arranged by size:
2051 1794 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3114 3146 7536 7568 3114
Size: 32 4390 32 3122
Fragments arranged by size:
4390 3122 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2947 4375 1316
Size: 1631 1428 4517
Fragments arranged by size:
4517 1631 1428
XbaI T'CTAG_A
Cuts at: 2612 4379 4912 2612
Size: 1767 533 5276
Fragments arranged by size:
5276 1767 533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2461
Size: 7576
XhoI C'TCGA_G
Cuts at: 1943 3827 1943
Size: 1884 5692
XmaI C'CCGG_G
Cuts at: 1870 3531 4901 1870
Size: 1661 1370 4545
Fragments arranged by size:
4545 1661 1370
XmnI GAAnn'nnTTC
Cuts at: 5705 5705
Size: 7576
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5384 276
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI
RsrII SacI SanDI SexAI SfoI SrfI SwaI