Restriction map for
pFN21AA0520
(Circular) (Six-base) MAPSORT of: pFN21AA0520.seq Check: 1231 from: 1 to: 8764
pFN21AA0520 8764 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4424 5415 4424
Size: 991 7773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4548 6574 278
Size: 53 83 186 936 3012 2026 2468
Fragments arranged by size:
3012 2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4385 4644 6107 1937
Size: 2448 259 1463 4594
Fragments arranged by size:
4594 2448 1463 259
Acc65I G'GTAC_C
Cuts at: 6085 6085
Size: 8764
AclI AA'CG_TT
Cuts at: 1563 6048 6891 7264 1563
Size: 4485 843 373 3063
Fragments arranged by size:
4485 3063 843 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3948 4342 4445 4459 5156 5188 6827 8211
Size: 2316 394 103 14 697 32 1639 1384
Cuts at: 8211 8479 1632
Size: 268 1917
Fragments arranged by size:
2316 1917 1639 1384 697 394 268 103
32 14
AfeI AGC'GCT
Cuts at: 2324 2324
Size: 8764
AflII C'TTAA_G
Cuts at: 820 1017 3884 820
Size: 197 2867 5700
Fragments arranged by size:
5700 2867 197
AflIII A'CryG_T
Cuts at: 1203 2520 3528 7663 8640 1203
Size: 1317 1008 4135 977 1327
Fragments arranged by size:
4135 1327 1317 1008 977
AgeI A'CCGG_T
Cuts at: 3627 7595 3627
Size: 3968 4796
AhdI GACnn_n'nnGTC
Cuts at: 2432 3532 7493 2432
Size: 1100 3961 3703
Fragments arranged by size:
3961 3703 1100
AleI CACnn'nnGTG
Cuts at: 1166 2051 4781 5606 1166
Size: 885 2730 825 4324
Fragments arranged by size:
4324 2730 885 825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2102 2708 2876 1184
Size: 918 606 168 7072
Fragments arranged by size:
7072 918 606 168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 8732
AlwNI CAG_nnn'CTG
Cuts at: 2029 2032 2271 4279 4755 5416 6507 8079 2029
Size: 3 239 2008 476 661 1091 1572 2714
Fragments arranged by size:
2714 2008 1572 1091 661 476 239 3
ApaI G_GGCC'C
Cuts at: 2833 5517 2833
Size: 2684 6080
ApaLI G'TGCA_C
Cuts at: 3473 4724 5737 6821 7977 3473
Size: 1251 1013 1084 1156 4260
Fragments arranged by size:
4260 1251 1156 1084 1013
ApoI r'AATT_y
Cuts at: 1493 3070 3521 4867 5000 6073 6299 6451 8560
Size: 1577 451 1346 133 1073 226 152 2109
Cuts at: 8560 1493
Size: 1697
Fragments arranged by size:
2109 1697 1577 1346 1073 451 226 152
133
AseI AT'TA_AT
Cuts at: 160 1032 4245 7318 160
Size: 872 3213 3073 1606
Fragments arranged by size:
3213 3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
AvaI C'yCGr_G
Cuts at: 1870 1943 3094 3359 3366 6089 1870
Size: 73 1151 265 7 2723 4545
Fragments arranged by size:
4545 2723 1151 265 73 7
AvrII C'CTAG_G
Cuts at: 3812 3812
Size: 8764
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2714 2747 2714
Size: 33 8731
BamHI G'GATC_C
Cuts at: 6094 6461 6094
Size: 367 8397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3482 3935 4903 5537 5848
Size: 325 217 627 1695 453 968 634 311
Cuts at: 5848 6085 7540 618
Size: 237 1455 1842
Fragments arranged by size:
1842 1695 1455 968 634 627 453 325
311 237 217
BanII G_rGCy'C
Cuts at: 1391 2833 3101 3366 4032 5268 5289 5517 5949
Size: 1442 268 265 666 1236 21 228 432
Cuts at: 5949 6083 1391
Size: 134 4072
Fragments arranged by size:
4072 1442 1236 666 432 268 265 228
134 21
BbeI G_GCGC'C
Cuts at: 3939 3939
Size: 8764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3114 3710 4212 4343 4465 4951 5723
Size: 971 1215 596 502 131 122 486 772
Cuts at: 5723 928
Size: 3969
Fragments arranged by size:
3969 1215 971 772 596 502 486 131
122
BbvCI CC'TCA_GC
Cuts at: 4034 4148 5099 4034
Size: 114 951 7699
Fragments arranged by size:
7699 951 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6955 6989 1903
Size: 34 5018 34 3678
Fragments arranged by size:
5018 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6656 7866 6656
Size: 1210 7554
BclI T'GATC_A
Cuts at: 1548 3411 1548
Size: 1863 6901
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4466 4938 7375
Size: 107 122 71 631 605 2794 472 2437
Cuts at: 7375 136
Size: 1525
Fragments arranged by size:
2794 2437 1525 631 605 472 122 107
71
BglII A'GATC_T
Cuts at: 4086 8759 4086
Size: 4673 4091
BlpI GC'TnA_GC
Cuts at: 2849 4748 5127 6181 2849
Size: 1899 379 1054 5432
Fragments arranged by size:
5432 1899 1054 379
Bme1580I G_kGCm'C
Cuts at: 1790 2833 3477 3485 4728 4826 5517 5741 6825
Size: 1043 644 8 1243 98 691 224 1084
Cuts at: 6825 7981 1790
Size: 1156 2573
Fragments arranged by size:
2573 1243 1156 1084 1043 691 644 224
98 8
BmgBI CAC'GTC
Cuts at: 1318 3529 4598 5038 1318
Size: 2211 1069 440 5044
Fragments arranged by size:
5044 2211 1069 440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2848 4682 6495
Size: 416 53 83 399 24 1431 1834 1813
Cuts at: 6495 7453 442
Size: 958 1753
Fragments arranged by size:
1834 1813 1753 1431 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 8764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 2248 2324 2339 4020 4301 4514 4559
Size: 811 198 76 15 1681 281 213 45
Cuts at: 4559 4592 7424 1239
Size: 33 2832 2579
Fragments arranged by size:
2832 2579 1681 811 281 213 198 76
45 33 15
Bpu10I CC'TnA_GC
Cuts at: 4034 4148 5099 5291 4034
Size: 114 951 192 7507
Fragments arranged by size:
7507 951 192 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2646 2921 5671 6889 7754 8052 2646
Size: 275 2750 1218 865 298 3358
Fragments arranged by size:
3358 2750 1218 865 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 3260 3293 4664 5826 5926 7427
Size: 617 1269 492 33 1371 1162 100 1501
Cuts at: 7427 882
Size: 2219
Fragments arranged by size:
2219 1501 1371 1269 1162 617 492 100
33
BsaAI yAC'GTr
Cuts at: 493 1204 5122 5343 6555 493
Size: 711 3918 221 1212 2702
Fragments arranged by size:
3918 2702 1212 711 221
BsaBI GATnn'nnATC
Cuts at: 8758 8758
Size: 8764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 2591 3936 4179
Size: 53 83 186 936 657 401 1345 243
Cuts at: 4179 4545 5354 6571 6953 7588 275
Size: 366 809 1217 382 635 1451
Fragments arranged by size:
1451 1345 1217 936 809 657 635 401
382 366 243 186 83 53
BsaWI w'CCGG_w
Cuts at: 2352 3627 7197 7595 7869 8016 8427 2352
Size: 1275 3570 398 274 147 411 2689
Fragments arranged by size:
3570 2689 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 3290 3320 3901
Size: 30 165 30 735 30 1122 30 581
Cuts at: 3901 3931 4432 4462 4487 4517 5559 5589 1178
Size: 30 501 30 25 30 1042 30 4353
Fragments arranged by size:
4353 1122 1042 735 581 501 165 30
30 30 30 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2321 3074 3311 3526 3960 4022 4064
Size: 960 175 753 237 215 434 62 42
Cuts at: 4064 5234 1186
Size: 1170 4716
Fragments arranged by size:
4716 1170 960 753 434 237 215 175
62 42
BseYI C'CCAG_C
Cuts at: 2244 2340 3571 3664 7967 8463 2244
Size: 96 1231 93 4303 496 2545
Fragments arranged by size:
4303 2545 1231 496 96 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 3774 4500 4540 1163
Size: 573 1083 955 726 40 5387
Fragments arranged by size:
5387 1083 955 726 573 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 3286 4707 5526 6214 6975
Size: 275 186 1276 9 1421 819 688 761
Cuts at: 6975 8003 1540
Size: 1028 2301
Fragments arranged by size:
2301 1421 1276 1028 819 761 688 275
186 9
BsiHKAI G_wGCw'C
Cuts at: 2697 2930 3477 3952 4728 5268 5289 5406 5741
Size: 233 547 475 776 540 21 117 335
Cuts at: 5741 6825 6910 7981 2697
Size: 1084 85 1071 3480
Fragments arranged by size:
3480 1084 1071 776 547 540 475 335
233 117 85 21
BsmI GAATG_Cn'
Cuts at: 6275 6368 6275
Size: 93 8671
BsmBI CGTCTCn'nnnn_
Cuts at: 4819 5028 4819
Size: 209 8555
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2697 2833 2930 3101 3366 3477 3485
Size: 399 907 136 97 171 265 111 8
Cuts at: 3485 3952 4032 4728 4826 5268 5289 5406 5517
Size: 467 80 696 98 442 21 117 111
Cuts at: 5517 5741 5949 6083 6825 6910 7981 1391
Size: 224 208 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 696 467 442 399
265 224 208 171 136 134 117 111
111 98 97 85 80 21 8
BspEI T'CCGG_A
Cuts at: 8427 8427
Size: 8764
BspHI T'CATG_A
Cuts at: 6653 8383 6653
Size: 1730 7034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3899 4424 5415 6119 844
Size: 3055 525 991 704 3489
Fragments arranged by size:
3489 3055 991 704 525
BsrBI CCG'CTC
Cuts at: 1394 1823 6651 1394
Size: 429 4828 3507
Fragments arranged by size:
4828 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7259 7433 65
Size: 1183 6011 174 1396
Fragments arranged by size:
6011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 2346 3581 3627 3932 4583 5603
Size: 110 407 129 1235 46 305 651 1020
Cuts at: 5603 7408 7595 1700
Size: 1805 187 2869
Fragments arranged by size:
2869 1805 1235 1020 651 407 305 187
129 110 46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8764
BssSI C'ACGA_G
Cuts at: 6824 7836 6824
Size: 1012 7752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 5244 794
Size: 1238 3212 4314
Fragments arranged by size:
4314 3212 1238
BstBI TT'CG_AA
Cuts at: 7568 7568
Size: 8764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8764
BstXI CCAn_nnnn'nTGG
Cuts at: 3562 4525 4783 5347 5593 5608 5804 3562
Size: 963 258 564 246 15 196 6522
Fragments arranged by size:
6522 963 564 258 246 196 15
BstYI r'GATC_y
Cuts at: 1972 2420 4086 5047 5064 5412 6094 6461 6846
Size: 448 1666 961 17 348 682 367 385
Cuts at: 6846 6863 8315 8401 8413 8759 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1666 1452 961 682 448 385 367
348 346 86 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 3805 3805
Size: 8764
BtgI C'CryG_G
Cuts at: 513 1065 2004 2866 5029 5509 5936 513
Size: 552 939 862 2163 480 427 3341
Fragments arranged by size:
3341 2163 939 862 552 480 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5566 487
Size: 683 56 4340 3685
Fragments arranged by size:
4340 3685 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 4600 6280 7074 7094 2137
Size: 2463 1680 794 20 3807
Fragments arranged by size:
3807 2463 1680 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6042 6077 529
Size: 35 5478 35 3216
Fragments arranged by size:
5478 3216 35 35
DraI TTT'AAA
Cuts at: 3520 6068 3520
Size: 2548 6216
DraIII CAC_nnn'GTG
Cuts at: 4189 4189
Size: 8764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5792 7771 809
Size: 507 4476 1979 1802
Fragments arranged by size:
4476 1979 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 3552 3579
Size: 54 1425 50 275 18 798 924 27
Cuts at: 3579 4437 4638 4704 4939 5523 6211 7100 8729
Size: 858 201 66 235 584 688 889 1629
Cuts at: 8729 8
Size: 43
Fragments arranged by size:
1629 1425 924 889 858 798 688 584
275 235 201 66 54 50 43 27
18
EagI C'GGCC_G
Cuts at: 1537 1812 4704 5523 6211 1537
Size: 275 2892 819 688 4090
Fragments arranged by size:
4090 2892 819 688 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 4305 5628 6694 1353
Size: 1016 1936 1323 1066 3423
Fragments arranged by size:
3423 1936 1323 1066 1016
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2226 3140 7351 7725 7871 387
Size: 1064 138 637 914 4211 374 146 1280
Fragments arranged by size:
4211 1280 1064 914 637 374 146 138
EcoICRI GAG'CTC
Cuts at: 5266 5287 5266
Size: 21 8743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2248 2324 2339 3948 4020 4301
Size: 393 418 198 76 15 1609 72 281
Cuts at: 4301 4342 4445 4459 4514 4559 4592 5156 5188
Size: 41 103 14 55 45 33 564 32
Cuts at: 5188 6827 7424 8211 8479 1239
Size: 1639 597 787 268 1524
Fragments arranged by size:
1639 1609 1524 787 597 564 418 393
281 268 198 103 76 72 55 45
41 33 32 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2598 3293 4271 4759 2306
Size: 292 695 978 488 6311
Fragments arranged by size:
6311 978 695 488 292
EcoO109I rG'GnC_Cy
Cuts at: 2259 2278 2829 2830 2968 3809 4004 4764 5254
Size: 19 551 1 138 841 195 760 490
Cuts at: 5254 5514 2259
Size: 260 5509
Fragments arranged by size:
5509 841 760 551 490 260 195 138
19 1
EcoRI G'AATT_C
Cuts at: 3070 6073 6451 3070
Size: 3003 378 5383
Fragments arranged by size:
5383 3003 378
EcoRV GAT'ATC
Cuts at: 3202 3717 3202
Size: 515 8249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5066 5066
Size: 8764
FspI TGC'GCA
Cuts at: 2225 2574 7270 2225
Size: 349 4696 3719
Fragments arranged by size:
4696 3719 349
HaeII r_GCGC'y
Cuts at: 1678 2326 2347 3939 4567 7911 1678
Size: 648 21 1592 628 3344 2531
Fragments arranged by size:
3344 2531 1592 648 628 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 2728 2760 3518
Size: 32 135 32 846 32 278 32 758
Cuts at: 3518 3550 3706 3738 4288 4320 4487 4519 5166
Size: 32 156 32 550 32 167 32 647
Cuts at: 5166 5198 5559 5591 5861 5893 7405 7437 7479
Size: 32 361 32 270 32 1512 32 42
Cuts at: 7479 7511 1373
Size: 32 2626
Fragments arranged by size:
2626 1512 846 758 647 550 361 278
270 167 156 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 5200 5578 6108 6354 669
Size: 988 281 2448 814 378 530 246 3079
Fragments arranged by size:
3079 2448 988 814 530 378 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8764
HpaI GTT'AAC
Cuts at: 6354 6354
Size: 8764
KasI G'GCGC_C
Cuts at: 3935 3935
Size: 8764
KpnI G_GTAC'C
Cuts at: 6089 6089
Size: 8764
MfeI C'AATT_G
Cuts at: 6363 6363
Size: 8764
MluI A'CGCG_T
Cuts at: 2520 8640 2520
Size: 6120 2644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2643 3561 7878 8062 1305
Size: 840 498 918 4317 184 2007
Fragments arranged by size:
4317 2007 918 840 498 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3554 10
Size: 54 1425 343 1722 5220
Fragments arranged by size:
5220 1722 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2690 3218 3395 4523 4619 4781
Size: 648 885 639 528 177 1128 96 162
Cuts at: 4781 5345 5606 5624 6722 7081 7240 518
Size: 564 261 18 1098 359 159 2042
Fragments arranged by size:
2042 1128 1098 885 648 639 564 528
359 261 177 162 159 96 18
MspA1I CmG'CkG
Cuts at: 1606 2029 2070 2113 2248 2271 3571 3750 4147
Size: 423 41 43 135 23 1300 179 397
Cuts at: 4147 4798 5134 5172 5770 6180 6857 8005 8250
Size: 651 336 38 598 410 677 1148 245
Cuts at: 8250 1606
Size: 2120
Fragments arranged by size:
2120 1300 1148 677 651 598 423 410
397 336 245 179 135 43 41 38
23
NaeI GCC'GGC
Cuts at: 2219 3583 3934 4585 2219
Size: 1364 351 651 6398
Fragments arranged by size:
6398 1364 651 351
NarI GG'CG_CC
Cuts at: 3936 3936
Size: 8764
NcoI C'CATG_G
Cuts at: 513 1065 2004 5509 513
Size: 552 939 3505 3768
Fragments arranged by size:
3768 3505 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8764
NgoMIV G'CCGG_C
Cuts at: 2217 3581 3932 4583 2217
Size: 1364 351 651 6398
Fragments arranged by size:
6398 1364 651 351
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8764
NotI GC'GGCC_GC
Cuts at: 6211 6211
Size: 8764
NruI TCG'CGA
Cuts at: 4998 4998
Size: 8764
NspI r_CATG'y
Cuts at: 1132 1229 3089 5077 5473 6122 7667 1132
Size: 97 1860 1988 396 649 1545 2229
Fragments arranged by size:
2229 1988 1860 1545 649 396 97
PasI CC'CwG_GG
Cuts at: 1348 3325 1348
Size: 1977 6787
PciI A'CATG_T
Cuts at: 7663 7663
Size: 8764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2271 3247 3676 4189 4948 5866 1108
Size: 1163 976 429 513 759 918 4006
Fragments arranged by size:
4006 1163 976 918 759 513 429
PfoI T'CCnGG_A
Cuts at: 2793 2921 4164 4266 4368 4536 4754 2793
Size: 128 1243 102 102 168 218 6803
Fragments arranged by size:
6803 1243 218 168 128 102 102
PmeI GTTT'AAAC
Cuts at: 6068 6068
Size: 8764
PmlI CAC'GTG
Cuts at: 5122 5122
Size: 8764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6833 6865 8346 8378 6833
Size: 32 1481 32 7219
Fragments arranged by size:
7219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2968 4004 4764 5254 2278
Size: 690 1036 760 490 5788
Fragments arranged by size:
5788 1036 760 690 490
PshAI GACnn'nnGTC
Cuts at: 1613 2594 1613
Size: 981 7783
PsiI TTA'TAA
Cuts at: 6334 6334
Size: 8764
PspOMI G'GGCC_C
Cuts at: 2829 5513 2829
Size: 2684 6080
PspXI vC'TCGA_Gb
Cuts at: 3094 3094
Size: 8764
PstI C_TGCA'G
Cuts at: 830 2168 3749 5412 6116 830
Size: 1338 1581 1663 704 3478
Fragments arranged by size:
3478 1663 1581 1338 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8764
PvuII CAG'CTG
Cuts at: 2029 2113 2248 3571 3750 5134 5770 2029
Size: 84 135 1323 179 1384 636 5023
Fragments arranged by size:
5023 1384 1323 636 179 135 84
SacI G_AGCT'C
Cuts at: 5268 5289 5268
Size: 21 8743
SalI G'TCGA_C
Cuts at: 1936 4384 6106 1936
Size: 2448 1722 4594
Fragments arranged by size:
4594 2448 1722
SanDI GG'GwC_CC
Cuts at: 2278 2278
Size: 8764
SapI GCTCTTCn'nnn_
Cuts at: 2369 2369
Size: 8764
SbfI CC_TGCA'GG
Cuts at: 6116 6116
Size: 8764
ScaI AGT'ACT
Cuts at: 7012 8724 7012
Size: 1712 7052
SexAI A'CCwGG_T
Cuts at: 5844 5844
Size: 8764
SfcI C'TryA_G
Cuts at: 826 1045 2164 3141 3745 4291 5305 5408 5677
Size: 219 1119 977 604 546 1014 103 269
Cuts at: 5677 6112 7247 7928 8119 826
Size: 435 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 1014 977 681 604 546
435 269 219 191 103
SfoI GGC'GCC
Cuts at: 3937 3937
Size: 8764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
SgrAI Cr'CCGG_yG
Cuts at: 1700 5603 1700
Size: 3903 4861
SmaI CCC'GGG
Cuts at: 1872 3368 6091 1872
Size: 1496 2723 4545
Fragments arranged by size:
4545 2723 1496
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 2936 3094 3884 5650 6868
Size: 197 926 718 275 158 790 1766 1218
Cuts at: 6868 7769 8031 820
Size: 901 262 1553
Fragments arranged by size:
1766 1553 1218 926 901 790 718 275
262 197 158
SnaBI TAC'GTA
Cuts at: 493 6555 493
Size: 6062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8764
SphI G_CATG'C
Cuts at: 1132 3089 5077 6122 1132
Size: 1957 1988 1045 3774
Fragments arranged by size:
3774 1988 1957 1045
SspI AAT'ATT
Cuts at: 5 52 6688 5
Size: 47 6636 2081
Fragments arranged by size:
6636 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 3761 1517
Size: 795 1449 6520
Fragments arranged by size:
6520 1449 795
StyI C'CwwG_G
Cuts at: 513 1065 2004 3732 3812 3990 5430 5509 5907
Size: 552 939 1728 80 178 1440 79 398
Cuts at: 5907 6203 513
Size: 296 3074
Fragments arranged by size:
3074 1728 1440 939 552 398 296 178
80 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3263 6791 6808 6961 7146 7591 325
Size: 1255 1683 3528 17 153 185 445 1498
Fragments arranged by size:
3528 1683 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4861 7010 8722
Size: 274 80 33 51 1443 2884 2149 1712
Cuts at: 8722 96
Size: 138
Fragments arranged by size:
2884 2149 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 3290 3322 3832 3864 3901 3933 4432
Size: 32 853 32 510 32 37 32 499
Cuts at: 4432 4464 5756 5788 8724 8756 2405
Size: 32 1292 32 2936 32 2413
Fragments arranged by size:
2936 2413 1292 853 510 499 37 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3829 4405 5573 1316
Size: 2513 576 1168 4507
Fragments arranged by size:
4507 2513 1168 576
XbaI T'CTAG_A
Cuts at: 6100 6100
Size: 8764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4567 4567
Size: 8764
XhoI C'TCGA_G
Cuts at: 1943 3094 1943
Size: 1151 7613
XmaI C'CCGG_G
Cuts at: 1870 3366 6089 1870
Size: 1496 2723 4545
Fragments arranged by size:
4545 2723 1496
XmnI GAAnn'nnTTC
Cuts at: 6893 6893
Size: 8764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4546 6572 276
Size: 53 83 186 936 3012 2026 2468
Fragments arranged by size:
3012 2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BfrBI BsiWI BstZ17I FseI FspAI NsiI PacI
PsrI RsrII SacII SfiI SrfI SwaI