Restriction map for
pFN21AA0519
(Circular) (Six-base) MAPSORT of: pFN21AA0519.seq Check: 6316 from: 1 to: 7462
pFN21AA0519 7462 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3209 3209
Size: 7462
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5272 278
Size: 53 83 186 936 3736 2468
Fragments arranged by size:
3736 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 4805 1937
Size: 655 2213 4594
Fragments arranged by size:
4594 2213 655
Acc65I G'GTAC_C
Cuts at: 4783 4783
Size: 7462
AclI AA'CG_TT
Cuts at: 1563 2679 5589 5962 1563
Size: 1116 2910 373 3063
Fragments arranged by size:
3063 2910 1116 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 3059 3173 3299 5525 6909 7177 1632
Size: 728 699 114 126 2226 1384 268 1917
Fragments arranged by size:
2226 1917 1384 728 699 268 126 114
AfeI AGC'GCT
Cuts at: 3963 3963
Size: 7462
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7265
AflIII A'CryG_T
Cuts at: 1203 6361 7338 1203
Size: 5158 977 1327
Fragments arranged by size:
5158 1327 977
AgeI A'CCGG_T
Cuts at: 6293 6293
Size: 7462
AhdI GACnn_n'nnGTC
Cuts at: 6191 6191
Size: 7462
AleI CACnn'nnGTG
Cuts at: 1166 3998 1166
Size: 2832 4630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3960 3992 3960
Size: 32 7430
AlwNI CAG_nnn'CTG
Cuts at: 2386 3187 3905 4409 4600 5205 6777 2386
Size: 801 718 504 191 605 1572 3071
Fragments arranged by size:
3071 1572 801 718 605 504 191
ApaLI G'TGCA_C
Cuts at: 5519 6675 5519
Size: 1156 6306
ApoI r'AATT_y
Cuts at: 1493 3194 3887 3950 4093 4771 4997 5149 7258
Size: 1701 693 63 143 678 226 152 2109
Cuts at: 7258 1493
Size: 1697
Fragments arranged by size:
2109 1701 1697 693 678 226 152 143
63
AseI AT'TA_AT
Cuts at: 160 1032 6016 160
Size: 872 4984 1606
Fragments arranged by size:
4984 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7462
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2526 3311 3981 4296 4787 1870
Size: 73 262 321 785 670 315 491 4545
Fragments arranged by size:
4545 785 670 491 321 315 262 73
BamHI G'GATC_C
Cuts at: 2635 4792 5159 2635
Size: 2157 367 4938
Fragments arranged by size:
4938 2157 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 3377 3457 4783 6238
Size: 325 217 627 1220 370 80 1326 1455
Cuts at: 6238 618
Size: 1842
Fragments arranged by size:
1842 1455 1326 1220 627 370 325 217
80
BanII G_rGCy'C
Cuts at: 1391 2283 2439 2982 3899 4781 1391
Size: 892 156 543 917 882 4072
Fragments arranged by size:
4072 917 892 882 543 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 928
Size: 971 220 6271
Fragments arranged by size:
6271 971 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5653 5687 1903
Size: 34 3716 34 3678
Fragments arranged by size:
3716 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5354 6564 5354
Size: 1210 6252
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2800 2897 6073
Size: 107 122 71 631 605 1128 97 3176
Cuts at: 6073 136
Size: 1525
Fragments arranged by size:
3176 1525 1128 631 605 122 107 97
71
BglII A'GATC_T
Cuts at: 7457 7457
Size: 7462
BlpI GC'TnA_GC
Cuts at: 2099 2766 4879 2099
Size: 667 2113 4682
Fragments arranged by size:
4682 2113 667
Bme1580I G_kGCm'C
Cuts at: 1790 3244 5523 6679 1790
Size: 1454 2279 1156 2573
Fragments arranged by size:
2573 2279 1454 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2896 5193 6151
Size: 416 53 83 399 24 1479 2297 958
Cuts at: 6151 442
Size: 1753
Fragments arranged by size:
2297 1753 1479 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 4311 3103
Size: 1208 6254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2375 2792 3810 6122 1239
Size: 1073 63 417 1018 2312 2579
Fragments arranged by size:
2579 2312 1073 1018 417 63
Bpu10I CC'TnA_GC
Cuts at: 3898 3898
Size: 7462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 5587 6452 6750 2381
Size: 3206 865 298 3093
Fragments arranged by size:
3206 3093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6125 882
Size: 617 4626 2219
Fragments arranged by size:
4626 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2094 2239 2719 2843 5253 493
Size: 711 890 145 480 124 2410 2702
Fragments arranged by size:
2702 2410 890 711 480 145 124
BsaBI GATnn'nnATC
Cuts at: 7456 7456
Size: 7462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4140 5269 5651 6286
Size: 53 83 186 936 2607 1129 382 635
Cuts at: 6286 275
Size: 1451
Fragments arranged by size:
2607 1451 1129 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3135 5895 6293 6567 6714 7125 3135
Size: 2760 398 274 147 411 3472
Fragments arranged by size:
3472 2760 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 3960 3990 1178
Size: 30 165 30 1362 30 1165 30 4650
Fragments arranged by size:
4650 1362 1165 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7462
BseYI C'CCAG_C
Cuts at: 3221 3394 3625 3932 6665 7161 3221
Size: 173 231 307 2733 496 3522
Fragments arranged by size:
3522 2733 496 307 231 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3182 1163
Size: 573 1446 5443
Fragments arranged by size:
5443 1446 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4912 5673 6701 1540
Size: 275 186 2911 761 1028 2301
Fragments arranged by size:
2911 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2439 3826 4713 5523 5608 6679 2283
Size: 156 1387 887 810 85 1071 3066
Fragments arranged by size:
3066 1387 1071 887 810 156 85
BsmI GAATG_Cn'
Cuts at: 2422 4973 5066 2422
Size: 2551 93 4818
Fragments arranged by size:
4818 2551 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3734 3734
Size: 7462
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2439 2982 3244 3826 3899 4713
Size: 399 493 156 543 262 582 73 814
Cuts at: 4713 4781 5523 5608 6679 1391
Size: 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 814 742 582 543 493 399
262 156 85 73 68
BspEI T'CCGG_A
Cuts at: 7125 7125
Size: 7462
BspHI T'CATG_A
Cuts at: 4606 5351 7081 4606
Size: 745 1730 4987
Fragments arranged by size:
4987 1730 745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 2394 3209 3394 4817 844
Size: 1222 328 815 185 1423 3489
Fragments arranged by size:
3489 1423 1222 815 328 185
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 3087 3256 3632 4018 4630 5349
Size: 429 314 950 169 376 386 612 719
Cuts at: 5349 1394
Size: 3507
Fragments arranged by size:
3507 950 719 612 429 386 376 314
169
BsrDI GCAATG_nn'
Cuts at: 65 1248 3141 3979 4503 5957 6131 65
Size: 1183 1893 838 524 1454 174 1396
Fragments arranged by size:
1893 1454 1396 1183 838 524 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2501 2535 2746 3010 6106 6293 1700
Size: 110 691 34 211 264 3096 187 2869
Fragments arranged by size:
3096 2869 691 264 211 187 110 34
BsrGI T'GTAC_A
Cuts at: 96 4683 96
Size: 4587 2875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7462
BssSI C'ACGA_G
Cuts at: 2670 3468 4625 5522 6534 2670
Size: 798 1157 897 1012 3598
Fragments arranged by size:
3598 1157 1012 897 798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 794
Size: 1592 5870
BstBI TT'CG_AA
Cuts at: 3093 3360 6266 3093
Size: 267 2906 4289
Fragments arranged by size:
4289 2906 267
BstEII G'GTnAC_C
Cuts at: 1184 4555 1184
Size: 3371 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 2989 3892 4153 2989
Size: 903 261 6298
Fragments arranged by size:
6298 903 261
BstYI r'GATC_y
Cuts at: 1972 2245 2635 3621 3660 4792 5159 5544 5561
Size: 273 390 986 39 1132 367 385 17
Cuts at: 5561 7013 7099 7111 7457 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1132 986 390 385 367 346
273 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 4593 4593
Size: 7462
BtgI C'CryG_G
Cuts at: 513 1065 2892 4392 513
Size: 552 1827 1500 3583
Fragments arranged by size:
3583 1827 1500 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 2689 487
Size: 683 56 899 564 5260
Fragments arranged by size:
5260 899 683 564 56
BtsI GCAGTG_nn'
Cuts at: 3440 3567 4978 5772 5792 3440
Size: 127 1411 794 20 5110
Fragments arranged by size:
5110 1411 794 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7427
DraI TTT'AAA
Cuts at: 4766 4766
Size: 7462
DraIII CAC_nnn'GTG
Cuts at: 3248 3248
Size: 7462
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2615 4139 6469 809
Size: 507 1299 1524 2330 1802
Fragments arranged by size:
2330 1802 1524 1299 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 4321 4909
Size: 54 1425 50 275 18 1054 1437 588
Cuts at: 4909 5798 7427 8
Size: 889 1629 43
Fragments arranged by size:
1629 1437 1425 1054 889 588 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4909 1537
Size: 275 3097 4090
Fragments arranged by size:
4090 3097 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2354 3035 3090 3362 3639 4718 5392
Size: 918 83 681 55 272 277 1079 674
Cuts at: 5392 1353
Size: 3423
Fragments arranged by size:
3423 1079 918 681 674 277 272 83
55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 2818 4241 6049 6423 6569
Size: 1064 138 486 743 1423 1808 374 146
Cuts at: 6569 387
Size: 1280
Fragments arranged by size:
1808 1423 1280 1064 743 486 374 146
138
EcoICRI GAG'CTC
Cuts at: 2281 2437 2281
Size: 156 7306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2360 2375 2792 3059 3173 3299
Size: 393 680 48 15 417 267 114 126
Cuts at: 3299 3810 5525 6122 6909 7177 1239
Size: 511 1715 597 787 268 1524
Fragments arranged by size:
1715 1524 787 680 597 511 417 393
268 267 126 114 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3077 4073 3077
Size: 996 6466
EcoO109I rG'GnC_Cy
Cuts at: 2383 3184 3316 3718 3830 4117 4597 2383
Size: 801 132 402 112 287 480 5248
Fragments arranged by size:
5248 801 480 402 287 132 112
EcoRI G'AATT_C
Cuts at: 3194 4093 4771 5149 3194
Size: 899 678 378 5507
Fragments arranged by size:
5507 899 678 378
FspI TGC'GCA
Cuts at: 5968 5968
Size: 7462
HaeII r_GCGC'y
Cuts at: 1678 2305 3965 6609 1678
Size: 627 1660 2644 2531
Fragments arranged by size:
2644 2531 1660 627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 3043 3075 3595
Size: 32 135 32 613 32 826 32 520
Cuts at: 3595 3627 6103 6135 6177 6209 1373
Size: 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 826 613 520 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4008 4806 5052 669
Size: 988 281 2070 798 246 3079
Fragments arranged by size:
3079 2070 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7462
HpaI GTT'AAC
Cuts at: 5052 5052
Size: 7462
KpnI G_GTAC'C
Cuts at: 4787 4787
Size: 7462
MfeI C'AATT_G
Cuts at: 5061 5061
Size: 7462
MluI A'CGCG_T
Cuts at: 7338 7338
Size: 7462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2500 4395 6576 6760 1305
Size: 795 400 1895 2181 184 2007
Fragments arranged by size:
2181 2007 1895 795 400 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5640
Fragments arranged by size:
5640 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2820 3998 4151 4508 4553 4576 5420
Size: 648 1654 1178 153 357 45 23 844
Cuts at: 5420 5779 5938 518
Size: 359 159 2042
Fragments arranged by size:
2042 1654 1178 844 648 359 357 159
153 45 23
MspA1I CmG'CkG
Cuts at: 1606 2290 2959 3656 3813 4878 5555 6703 6948
Size: 684 669 697 157 1065 677 1148 245
Cuts at: 6948 1606
Size: 2120
Fragments arranged by size:
2120 1148 1065 697 684 677 669 245
157
NaeI GCC'GGC
Cuts at: 2503 2537 2503
Size: 34 7428
NcoI C'CATG_G
Cuts at: 513 1065 2892 4392 513
Size: 552 1827 1500 3583
Fragments arranged by size:
3583 1827 1500 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7462
NgoMIV G'CCGG_C
Cuts at: 2501 2535 2501
Size: 34 7428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7462
NotI GC'GGCC_GC
Cuts at: 4909 4909
Size: 7462
NspI r_CATG'y
Cuts at: 1132 1229 2065 3430 4675 4820 6365 1132
Size: 97 836 1365 1245 145 1545 2229
Fragments arranged by size:
2229 1545 1365 1245 836 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7462
PciI A'CATG_T
Cuts at: 6361 6361
Size: 7462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7462
PfoI T'CCnGG_A
Cuts at: 2259 3639 4467 2259
Size: 1380 828 5254
Fragments arranged by size:
5254 1380 828
PmeI GTTT'AAAC
Cuts at: 4766 4766
Size: 7462
PmlI CAC'GTG
Cuts at: 2094 2239 2094
Size: 145 7317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3960 3992 5531 5563 7044 7076 3960
Size: 32 1539 32 1481 32 4346
Fragments arranged by size:
4346 1539 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2383 3184 3316 3718 4117 2383
Size: 801 132 402 399 5728
Fragments arranged by size:
5728 801 402 399 132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7462
PsiI TTA'TAA
Cuts at: 5032 5032
Size: 7462
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 7430
PstI C_TGCA'G
Cuts at: 830 2289 3435 4814 830
Size: 1459 1146 1379 3478
Fragments arranged by size:
3478 1459 1379 1146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7462
PvuII CAG'CTG
Cuts at: 2290 3656 3813 2290
Size: 1366 157 5939
Fragments arranged by size:
5939 1366 157
SacI G_AGCT'C
Cuts at: 2283 2439 2283
Size: 156 7306
SalI G'TCGA_C
Cuts at: 1936 4804 1936
Size: 2868 4594
SanDI GG'GwC_CC
Cuts at: 3316 3316
Size: 7462
SapI GCTCTTCn'nnn_
Cuts at: 2271 2354 3362 4718 2271
Size: 83 1008 1356 5015
Fragments arranged by size:
5015 1356 1008 83
SbfI CC_TGCA'GG
Cuts at: 4814 4814
Size: 7462
ScaI AGT'ACT
Cuts at: 2881 5710 7422 2881
Size: 2829 1712 2921
Fragments arranged by size:
2921 2829 1712
SfcI C'TryA_G
Cuts at: 826 1045 2285 3431 4810 5945 6626 6817 826
Size: 219 1240 1146 1379 1135 681 191 1471
Fragments arranged by size:
1471 1379 1240 1146 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7462
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7462
SmaI CCC'GGG
Cuts at: 1872 2207 3313 4789 1872
Size: 335 1106 1476 4545
Fragments arranged by size:
4545 1476 1106 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 5566 6467 6729 820
Size: 197 926 453 3170 901 262 1553
Fragments arranged by size:
3170 1553 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 5253 493
Size: 4760 2702
SpeI A'CTAG_T
Cuts at: 152 2725 152
Size: 2573 4889
SphI G_CATG'C
Cuts at: 1132 2065 4820 1132
Size: 933 2755 3774
Fragments arranged by size:
3774 2755 933
SspI AAT'ATT
Cuts at: 5 52 5386 5
Size: 47 5334 2081
Fragments arranged by size:
5334 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7462
StyI C'CwwG_G
Cuts at: 513 1065 2640 2892 4392 4901 513
Size: 552 1575 252 1500 509 3074
Fragments arranged by size:
3074 1575 1500 552 509 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 3330 3778 5489 5506 5659 5844
Size: 1255 852 898 448 1711 17 153 185
Cuts at: 5844 6289 325
Size: 445 1498
Fragments arranged by size:
1711 1498 1255 898 852 448 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2879 2925 4683
Size: 274 80 33 51 1443 902 46 1758
Cuts at: 4683 5708 7420 96
Size: 1025 1712 138
Fragments arranged by size:
1758 1712 1443 1025 902 274 138 80
51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2946 2978 4109 4141 7422 7454 2946
Size: 32 1131 32 3281 32 2954
Fragments arranged by size:
3281 2954 1131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 1316
Size: 1629 5833
XbaI T'CTAG_A
Cuts at: 4798 4798
Size: 7462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2618 2930 4471 2618
Size: 312 1541 5609
Fragments arranged by size:
5609 1541 312
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7462
XmaI C'CCGG_G
Cuts at: 1870 2205 3311 4787 1870
Size: 335 1106 1476 4545
Fragments arranged by size:
4545 1476 1106 335
XmnI GAAnn'nnTTC
Cuts at: 5591 5591
Size: 7462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5270 276
Size: 53 83 186 936 3736 2468
Fragments arranged by size:
3736 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI RsrII SacII SexAI SfiI
SfoI SrfI SwaI