Restriction map for
pFN21AA0476
(Circular) (Six-base) MAPSORT of: pFN21AA0476.seq Check: 7050 from: 1 to: 9193
pFN21AA0476 9193 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4729 6285 4729
Size: 1556 7637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7003 278
Size: 53 83 186 936 5467 2468
Fragments arranged by size:
5467 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3480 6536 1937
Size: 1543 3056 4594
Fragments arranged by size:
4594 3056 1543
Acc65I G'GTAC_C
Cuts at: 6514 6514
Size: 9193
AclI AA'CG_TT
Cuts at: 1563 3084 7320 7693 1563
Size: 1521 4236 373 3063
Fragments arranged by size:
4236 3063 1521 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4799 7256 8640 8908 1632
Size: 3167 2457 1384 268 1917
Fragments arranged by size:
3167 2457 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8996
AflIII A'CryG_T
Cuts at: 1203 8092 9069 1203
Size: 6889 977 1327
Fragments arranged by size:
6889 1327 977
AgeI A'CCGG_T
Cuts at: 8024 8024
Size: 9193
AhdI GACnn_n'nnGTC
Cuts at: 7922 7922
Size: 9193
AleI CACnn'nnGTG
Cuts at: 1166 2150 2630 2786 1166
Size: 984 480 156 7573
Fragments arranged by size:
7573 984 480 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2954 3137 4445 5879 1184
Size: 1770 183 1308 1434 4498
Fragments arranged by size:
4498 1770 1434 1308 183
AlwNI CAG_nnn'CTG
Cuts at: 3208 3217 3312 3598 3874 4277 4381 6035 6152
Size: 9 95 286 276 403 104 1654 117
Cuts at: 6152 6936 8508 3208
Size: 784 1572 3893
Fragments arranged by size:
3893 1654 1572 784 403 286 276 117
104 95 9
ApaI G_GGCC'C
Cuts at: 2115 2582 3224 3685 4174 4297 6258 2115
Size: 467 642 461 489 123 1961 5050
Fragments arranged by size:
5050 1961 642 489 467 461 123
ApaLI G'TGCA_C
Cuts at: 6218 7250 8406 6218
Size: 1032 1156 7005
Fragments arranged by size:
7005 1156 1032
ApoI r'AATT_y
Cuts at: 1493 3944 5457 6502 6728 6880 8989 1493
Size: 2451 1513 1045 226 152 2109 1697
Fragments arranged by size:
2451 2109 1697 1513 1045 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7747 160
Size: 872 6715 1606
Fragments arranged by size:
6715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9193
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2693 3837 4061 4368 4905 4974
Size: 73 474 276 1144 224 307 537 69
Cuts at: 4974 5304 6518 1870
Size: 330 1214 4545
Fragments arranged by size:
4545 1214 1144 537 474 330 307 276
224 73 69
BamHI G'GATC_C
Cuts at: 5318 5432 6523 6890 5318
Size: 114 1091 367 7621
Fragments arranged by size:
7621 1091 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 2822 3188 4299 4360
Size: 325 217 627 634 401 366 1111 61
Cuts at: 4360 5156 5177 5533 5651 6420 6514 7969 618
Size: 796 21 356 118 769 94 1455 1842
Fragments arranged by size:
1842 1455 1111 796 769 634 627 401
366 356 325 217 118 94 61 21
BanII G_rGCy'C
Cuts at: 1391 2115 2142 2582 3224 3685 3793 4041 4174
Size: 724 27 440 642 461 108 248 133
Cuts at: 4174 4297 4782 5066 5339 5739 5810 6237 6258
Size: 123 485 284 273 400 71 427 21
Cuts at: 6258 6512 1391
Size: 254 4072
Fragments arranged by size:
4072 724 642 485 461 440 427 400
284 273 254 248 133 123 108 71
27 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 2767 3745 5285 5387 928
Size: 971 802 66 978 1540 102 4734
Fragments arranged by size:
4734 1540 978 971 802 102 66
BbvCI CC'TCA_GC
Cuts at: 6416 6416
Size: 9193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4052 4086 5700 5734 7384 7418 1903
Size: 34 2115 34 1614 34 1650 34 3678
Fragments arranged by size:
3678 2115 1650 1614 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3175 3437 7085 8295 3175
Size: 262 3648 1210 4073
Fragments arranged by size:
4073 3648 1210 262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9193
BfrBI ATG'CAT
Cuts at: 2205 2205
Size: 9193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 4910 4937
Size: 107 122 71 631 605 354 2884 27
Cuts at: 4937 5061 7804 136
Size: 124 2743 1525
Fragments arranged by size:
2884 2743 1525 631 605 354 124 122
107 71 27
BglII A'GATC_T
Cuts at: 9188 9188
Size: 9193
BlpI GC'TnA_GC
Cuts at: 2136 2897 5071 5338 6610 2136
Size: 761 2174 267 1272 4719
Fragments arranged by size:
4719 2174 1272 761 267
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2173 2190 2424 2528 2582 2706 2772
Size: 325 58 17 234 104 54 124 66
Cuts at: 2772 2887 2923 3224 3359 3377 3406 3414 3685
Size: 115 36 301 135 18 29 8 271
Cuts at: 3685 4174 4297 4314 4542 4906 4915 4992 5086
Size: 489 123 17 228 364 9 77 94
Cuts at: 5086 5182 5536 5592 5757 6222 6258 7254 8410
Size: 96 354 56 165 465 36 996 1156
Cuts at: 8410 1790
Size: 2573
Fragments arranged by size:
2573 1156 996 489 465 364 354 325
301 271 234 228 165 135 124 123
115 104 96 94 77 66 58 56
54 36 36 29 18 17 17 9
8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 2183 2505
Size: 416 53 83 399 24 683 83 322
Cuts at: 2505 2897 2933 3401 4096 5080 5619 5940 6924
Size: 392 36 468 695 984 539 321 984
Cuts at: 6924 7882 442
Size: 958 1753
Fragments arranged by size:
1753 984 984 958 695 683 539 468
416 399 392 322 321 83 83 53
36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3928 3731
Size: 197 8996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2394 3418 3659 3710 3832 4535 5067 5852
Size: 1155 1024 241 51 122 703 532 785
Cuts at: 5852 6127 6468 7853 1239
Size: 275 341 1385 2579
Fragments arranged by size:
2579 1385 1155 1024 785 703 532 341
275 241 122 51
Bpu10I CC'TnA_GC
Cuts at: 3225 3993 4284 5018 5065 5241 5804 6416 3225
Size: 768 291 734 47 176 563 612 6002
Fragments arranged by size:
6002 768 734 612 563 291 176 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3722 4696 7318 8183 8481 3722
Size: 974 2622 865 298 4434
Fragments arranged by size:
4434 2622 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3100 4845 7856 882
Size: 617 1601 1745 3011 2219
Fragments arranged by size:
3011 2219 1745 1601 617
BsaAI yAC'GTr
Cuts at: 493 1204 6340 6984 493
Size: 711 5136 644 2702
Fragments arranged by size:
5136 2702 711 644
BsaBI GATnn'nnATC
Cuts at: 9187 9187
Size: 9193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4615 4873 7000 7382
Size: 53 83 186 936 3082 258 2127 382
Cuts at: 7382 8017 275
Size: 635 1451
Fragments arranged by size:
3082 2127 1451 936 635 382 258 186
83 53
BsaWI w'CCGG_w
Cuts at: 4635 7626 8024 8298 8445 8856 4635
Size: 2991 398 274 147 411 4972
Fragments arranged by size:
4972 2991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3008 3038 4727 4757 5819
Size: 30 165 30 1605 30 1689 30 1062
Cuts at: 5819 5849 1178
Size: 30 4522
Fragments arranged by size:
4522 1689 1605 1062 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2520 2681 3140 3515 3638 3920 4034
Size: 841 493 161 459 375 123 282 114
Cuts at: 4034 4079 4209 5048 6425 1186
Size: 45 130 839 1377 3954
Fragments arranged by size:
3954 1377 841 839 493 459 375 282
161 130 123 114 45
BseYI C'CCAG_C
Cuts at: 2051 2219 2317 2581 2650 4205 4306 4623 4835
Size: 168 98 264 69 1555 101 317 212
Cuts at: 4835 4855 5173 5266 5529 5633 5739 6129 8396
Size: 20 318 93 263 104 106 390 2267
Cuts at: 8396 8892 2051
Size: 496 2352
Fragments arranged by size:
2352 2267 1555 496 390 318 317 264
263 212 168 106 104 101 98 93
69 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2820 3143 3329 3860 4367 4394 5282
Size: 573 1084 323 186 531 507 27 888
Cuts at: 5282 6257 1163
Size: 975 4099
Fragments arranged by size:
4099 1084 975 888 573 531 507 323
186 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4358 6643 7404 8432 1540
Size: 275 186 2357 2285 761 1028 2301
Fragments arranged by size:
2357 2301 2285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 2793 3258 3267 3793 3914 4041 4434 5339
Size: 719 465 9 526 121 127 393 905
Cuts at: 5339 5703 6105 6222 6481 7254 7339 8410 2074
Size: 364 402 117 259 773 85 1071 2857
Fragments arranged by size:
2857 1071 905 773 719 526 465 402
393 364 259 127 121 117 85 9
BsmI GAATG_Cn'
Cuts at: 5229 6494 6704 6797 5229
Size: 1265 210 93 7625
Fragments arranged by size:
7625 1265 210 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3034 3331 3034
Size: 297 8896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2115 2142 2173 2190 2424 2528
Size: 399 284 41 27 31 17 234 104
Cuts at: 2528 2582 2706 2772 2793 2887 2923 3224 3258
Size: 54 124 66 21 94 36 301 34
Cuts at: 3258 3267 3359 3377 3406 3414 3685 3793 3914
Size: 9 92 18 29 8 271 108 121
Cuts at: 3914 4041 4174 4297 4314 4434 4542 4782 4906
Size: 127 133 123 17 120 108 240 124
Cuts at: 4906 4915 4992 5066 5086 5182 5339 5536 5592
Size: 9 77 74 20 96 157 197 56
Cuts at: 5592 5703 5739 5757 5810 6105 6222 6237 6258
Size: 111 36 18 53 295 117 15 21
Cuts at: 6258 6481 6512 7254 7339 8410 1391
Size: 223 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 295 284 271
240 234 223 197 157 133 127 124
124 123 121 120 117 111 108 108
104 96 94 92 85 77 74 66
56 54 53 41 36 36 34 31
31 29 27 21 21 20 18 18
17 17 15 9 9 8
BspEI T'CCGG_A
Cuts at: 4635 8856 4635
Size: 4221 4972
BspHI T'CATG_A
Cuts at: 7082 8812 7082
Size: 1730 7463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3306 4729 5265 5570 6166 6285 6548 844
Size: 2462 1423 536 305 596 119 263 3489
Fragments arranged by size:
3489 2462 1423 596 536 305 263 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 2911 4419 5041 7080 1394
Size: 429 194 894 1508 622 2039 3507
Fragments arranged by size:
3507 2039 1508 894 622 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 7688 7862 65
Size: 1183 6440 174 1396
Fragments arranged by size:
6440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 2544 2946 4931 6423 7837 8024
Size: 110 635 99 402 1985 1492 1414 187
Cuts at: 8024 1700
Size: 2869
Fragments arranged by size:
2869 1985 1492 1414 635 402 187 110
99
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9193
BssSI C'ACGA_G
Cuts at: 3792 7253 8265 3792
Size: 3461 1012 4720
Fragments arranged by size:
4720 3461 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3208 3271 4381 4492 794
Size: 2414 63 1110 111 5495
Fragments arranged by size:
5495 2414 1110 111 63
BstBI TT'CG_AA
Cuts at: 7997 7997
Size: 9193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9193
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 3348 6012 6400 2629
Size: 719 2664 388 5422
Fragments arranged by size:
5422 2664 719 388
BstYI r'GATC_y
Cuts at: 1972 5318 5432 6123 6523 6890 7275 7292 8744
Size: 3346 114 691 400 367 385 17 1452
Cuts at: 8744 8830 8842 9188 1972
Size: 86 12 346 1977
Fragments arranged by size:
3346 1977 1452 691 400 385 367 346
114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2082 3621 2082
Size: 1539 7654
BtgI C'CryG_G
Cuts at: 513 1065 2004 2721 2904 5283 513
Size: 552 939 717 183 2379 4423
Fragments arranged by size:
4423 2379 939 717 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2146 2941 487
Size: 683 56 920 795 6739
Fragments arranged by size:
6739 920 795 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 3239 3341 3359 3672 4093 4312 4379 4906
Size: 647 102 18 313 421 219 67 527
Cuts at: 4906 5322 5359 5863 6709 7503 7523 2592
Size: 416 37 504 846 794 20 4262
Fragments arranged by size:
4262 846 794 647 527 504 421 416
313 219 102 67 37 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5116 5151 529
Size: 35 4552 35 4571
Fragments arranged by size:
4571 4552 35 35
DraI TTT'AAA
Cuts at: 6497 6497
Size: 9193
DraIII CAC_nnn'GTG
Cuts at: 4610 4610
Size: 9193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8200 809
Size: 507 6884 1802
Fragments arranged by size:
6884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 3290 3570
Size: 54 1425 50 275 18 299 1161 280
Cuts at: 3570 3673 4592 6143 6390 6640 7529 9158 8
Size: 103 919 1551 247 250 889 1629 43
Fragments arranged by size:
1629 1551 1425 1161 919 889 299 280
275 250 247 103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6640 1537
Size: 275 4828 4090
Fragments arranged by size:
4828 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3506 4158 4818 4877 7123 1353
Size: 2153 652 660 59 2246 3423
Fragments arranged by size:
3423 2246 2153 660 652 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4655 7780 8154 8300 387
Size: 1064 138 435 2631 3125 374 146 1280
Fragments arranged by size:
3125 2631 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3791 4039 5337 3791
Size: 248 1298 7647
Fragments arranged by size:
7647 1298 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 3418 3659 3710 3832 4535 4799
Size: 393 762 1024 241 51 122 703 264
Cuts at: 4799 5067 5852 6127 6468 7256 7853 8640 8908
Size: 268 785 275 341 788 597 787 268
Cuts at: 8908 1239
Size: 1524
Fragments arranged by size:
1524 1024 788 787 785 762 703 597
393 341 275 268 268 264 241 122
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 4182 2615
Size: 1567 7626
EcoO109I rG'GnC_Cy
Cuts at: 2112 2414 2578 3035 3221 3319 3681 3775 4170
Size: 302 164 457 186 98 362 94 395
Cuts at: 4170 4171 4187 4963 5098 5123 5362 6001 6149
Size: 1 16 776 135 25 239 639 148
Cuts at: 6149 6254 6255 2112
Size: 105 1 5050
Fragments arranged by size:
5050 776 639 457 395 362 302 239
186 164 148 135 105 98 94 25
16 1 1
EcoRI G'AATT_C
Cuts at: 3944 6502 6880 3944
Size: 2558 378 6257
Fragments arranged by size:
6257 2558 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 6394 2374
Size: 4020 5173
FspI TGC'GCA
Cuts at: 2942 7699 2942
Size: 4757 4436
HaeII r_GCGC'y
Cuts at: 1678 2904 4203 4795 4822 5314 5416 8340 1678
Size: 1226 1299 592 27 492 102 2924 2531
Fragments arranged by size:
2924 2531 1299 1226 592 492 102 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 3099 3131 3617
Size: 32 135 32 489 32 1006 32 486
Cuts at: 3617 3649 4010 4042 4727 4759 4844 4876 5819
Size: 32 361 32 685 32 85 32 943
Cuts at: 5819 5851 6017 6049 6427 6459 7834 7866 7908
Size: 32 166 32 378 32 1375 32 42
Cuts at: 7908 7940 1373
Size: 32 2626
Fragments arranged by size:
2626 1375 1006 943 685 489 486 378
361 166 135 85 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3802 6537 6783 669
Size: 988 281 1864 2735 246 3079
Fragments arranged by size:
3079 2735 1864 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9193
HpaI GTT'AAC
Cuts at: 6783 6783
Size: 9193
KpnI G_GTAC'C
Cuts at: 6518 6518
Size: 9193
MfeI C'AATT_G
Cuts at: 6792 6792
Size: 9193
MluI A'CGCG_T
Cuts at: 9069 9069
Size: 9193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4560 5212 5953 6162 6184 8307 8491 1305
Size: 3255 652 741 209 22 2123 184 2007
Fragments arranged by size:
3255 2123 2007 741 652 209 184 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3572 6145 6392 10
Size: 54 1425 343 1740 2573 247 2811
Fragments arranged by size:
2811 2573 1740 1425 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2630 2786 2960 3119 3884 4317
Size: 648 984 480 156 174 159 765 433
Cuts at: 4317 7151 7510 7669 518
Size: 2834 359 159 2042
Fragments arranged by size:
2834 2042 984 765 648 480 433 359
174 159 159 156
MspA1I CmG'CkG
Cuts at: 1606 2135 2689 2804 2973 3205 3379 3598 3871
Size: 529 554 115 169 232 174 219 273
Cuts at: 3871 4255 4277 5194 5472 6133 6609 7286 8434
Size: 384 22 917 278 661 476 677 1148
Cuts at: 8434 8679 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 917 677 661 554 529 476
384 278 273 245 232 219 174 169
115 22
NaeI GCC'GGC
Cuts at: 2546 2948 2546
Size: 402 8791
NcoI C'CATG_G
Cuts at: 513 1065 2004 2721 5283 513
Size: 552 939 717 2562 4423
Fragments arranged by size:
4423 2562 939 717 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9193
NgoMIV G'CCGG_C
Cuts at: 2544 2946 2544
Size: 402 8791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9193
NotI GC'GGCC_GC
Cuts at: 6640 6640
Size: 9193
NsiI A_TGCA'T
Cuts at: 2207 2207
Size: 9193
NspI r_CATG'y
Cuts at: 1132 1229 2205 2477 2575 6551 8096 1132
Size: 97 976 272 98 3976 1545 2229
Fragments arranged by size:
3976 2229 1545 976 272 98 97
PasI CC'CwG_GG
Cuts at: 1348 2191 2479 5057 1348
Size: 843 288 2578 5484
Fragments arranged by size:
5484 2578 843 288
PciI A'CATG_T
Cuts at: 8092 8092
Size: 9193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 3598 6136 6266 1108
Size: 1125 1365 2538 130 4035
Fragments arranged by size:
4035 2538 1365 1125 130
PfoI T'CCnGG_A
Cuts at: 4965 4965
Size: 9193
PmeI GTTT'AAAC
Cuts at: 6497 6497
Size: 9193
PmlI CAC'GTG
Cuts at: 6340 6340
Size: 9193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7262 7294 8775 8807 7262
Size: 32 1481 32 7648
Fragments arranged by size:
7648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4963 5123 5362 4963
Size: 160 239 8794
Fragments arranged by size:
8794 239 160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9193
PsiI TTA'TAA
Cuts at: 6763 6763
Size: 9193
PspOMI G'GGCC_C
Cuts at: 2111 2578 3220 3681 4170 4293 6254 2111
Size: 467 642 461 489 123 1961 5050
Fragments arranged by size:
5050 1961 642 489 467 461 123
PstI C_TGCA'G
Cuts at: 830 2835 3204 3213 3822 6282 6545 830
Size: 2005 369 9 609 2460 263 3478
Fragments arranged by size:
3478 2460 2005 609 369 263 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9193
PvuII CAG'CTG
Cuts at: 2135 2804 3205 3598 3871 4277 5194 5472 2135
Size: 669 401 393 273 406 917 278 5856
Fragments arranged by size:
5856 917 669 406 401 393 278 273
SacI G_AGCT'C
Cuts at: 3793 4041 5339 3793
Size: 248 1298 7647
Fragments arranged by size:
7647 1298 248
SalI G'TCGA_C
Cuts at: 1936 6535 1936
Size: 4599 4594
SanDI GG'GwC_CC
Cuts at: 5123 5123
Size: 9193
SapI GCTCTTCn'nnn_
Cuts at: 3506 3506
Size: 9193
SbfI CC_TGCA'GG
Cuts at: 3822 6545 3822
Size: 2723 6470
ScaI AGT'ACT
Cuts at: 4106 7441 9153 4106
Size: 3335 1712 4146
Fragments arranged by size:
4146 3335 1712
SexAI A'CCwGG_T
Cuts at: 4606 4606
Size: 9193
SfcI C'TryA_G
Cuts at: 826 1045 2383 2831 3200 3209 3818 6278 6541
Size: 219 1338 448 369 9 609 2460 263
Cuts at: 6541 7676 8357 8548 826
Size: 1135 681 191 1471
Fragments arranged by size:
2460 1471 1338 1135 681 609 448 369
263 219 191 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4937 4937
Size: 9193
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9193
SgrAI Cr'CCGG_yG
Cuts at: 1700 6423 1700
Size: 4723 4470
SmaI CCC'GGG
Cuts at: 1872 2419 3839 4976 5306 6520 1872
Size: 547 1420 1137 330 1214 4545
Fragments arranged by size:
4545 1420 1214 1137 547 330
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3737 4675 7297 8198 8460 820
Size: 197 926 1794 938 2622 901 262 1553
Fragments arranged by size:
2622 1794 1553 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6984 493
Size: 6491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9193
SphI G_CATG'C
Cuts at: 1132 2477 6551 1132
Size: 1345 4074 3774
Fragments arranged by size:
4074 3774 1345
SspI AAT'ATT
Cuts at: 5 52 7117 5
Size: 47 7065 2081
Fragments arranged by size:
7065 2081 47
StuI AGG'CCT
Cuts at: 1517 4955 5961 1517
Size: 3438 1006 4749
Fragments arranged by size:
4749 3438 1006
StyI C'CwwG_G
Cuts at: 513 1065 2004 2232 2721 2852 3051 3703 4956
Size: 552 939 228 489 131 199 652 1253
Cuts at: 4956 5127 5283 6011 6447 6632 513
Size: 171 156 728 436 185 3074
Fragments arranged by size:
3074 1253 939 728 652 552 489 436
228 199 185 171 156 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 2246 3785 3990 5165 5724 6277
Size: 1255 509 157 1539 205 1175 559 553
Cuts at: 6277 7220 7237 7390 7575 8020 325
Size: 943 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 1175 943 559 553 509
445 205 185 157 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4104 6061 6366
Size: 274 80 33 51 1443 2127 1957 305
Cuts at: 6366 7439 9151 96
Size: 1073 1712 138
Fragments arranged by size:
2127 1957 1712 1443 1073 305 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4574 4606 4863 4895 4971 5003 9153 9185 4574
Size: 32 257 32 76 32 4150 32 4582
Fragments arranged by size:
4582 4150 257 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9193
XbaI T'CTAG_A
Cuts at: 6529 6529
Size: 9193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2216 3611 5098 2149
Size: 67 1395 1487 6244
Fragments arranged by size:
6244 1487 1395 67
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9193
XmaI C'CCGG_G
Cuts at: 1870 2417 3837 4974 5304 6518 1870
Size: 547 1420 1137 330 1214 4545
Fragments arranged by size:
4545 1420 1214 1137 547 330
XmnI GAAnn'nnTTC
Cuts at: 7322 7322
Size: 9193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7001 276
Size: 53 83 186 936 5467 2468
Fragments arranged by size:
5467 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI PspXI
PsrI RsrII SacII SfoI SrfI SwaI