Restriction map for
pFN21AA0474
(Circular) (Six-base) MAPSORT of: pFN21AA0474.seq Check: 803 from: 1 to: 6748
pFN21AA0474 6748 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 2391
Size: 6748
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2496 4558 278
Size: 53 83 186 936 960 2062 2468
Fragments arranged by size:
2468 2062 960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4091 1937
Size: 2154 4594
Acc65I G'GTAC_C
Cuts at: 4069 4069
Size: 6748
AclI AA'CG_TT
Cuts at: 1563 4875 5248 1563
Size: 3312 373 3063
Fragments arranged by size:
3312 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2620 4811 6195 6463 1632
Size: 988 2191 1384 268 1917
Fragments arranged by size:
2191 1917 1384 988 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6551
AflIII A'CryG_T
Cuts at: 1203 2805 5647 6624 1203
Size: 1602 2842 977 1327
Fragments arranged by size:
2842 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5579 5579
Size: 6748
AhdI GACnn_n'nnGTC
Cuts at: 5477 5477
Size: 6748
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3195 3227 3195
Size: 32 6716
AlwNI CAG_nnn'CTG
Cuts at: 4491 6063 4491
Size: 1572 5176
ApaI G_GGCC'C
Cuts at: 2984 3557 2984
Size: 573 6175
ApaLI G'TGCA_C
Cuts at: 2102 4805 5961 2102
Size: 2703 1156 2889
Fragments arranged by size:
2889 2703 1156
ApoI r'AATT_y
Cuts at: 1493 2678 3092 4057 4283 4435 6544 1493
Size: 1185 414 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 1185 965 414 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5302 160
Size: 872 4270 1606
Fragments arranged by size:
4270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2204 2246 3992 4073 1870
Size: 73 152 109 42 1746 81 4545
Fragments arranged by size:
4545 1746 152 109 81 73 42
BamHI G'GATC_C
Cuts at: 4078 4445 4078
Size: 367 6381
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 4069 5524 618
Size: 325 217 627 390 1892 1455 1842
Fragments arranged by size:
1892 1842 1455 627 390 325 217
BanII G_rGCy'C
Cuts at: 1391 2984 3536 3557 3629 3678 3707 4067 1391
Size: 1593 552 21 72 49 29 360 4072
Fragments arranged by size:
4072 1593 552 360 72 49 29 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 2496 2884 3650 928
Size: 971 436 161 388 766 4026
Fragments arranged by size:
4026 971 766 436 388 161
BbvCI CC'TCA_GC
Cuts at: 2970 2970
Size: 6748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4939 4973 1903
Size: 34 3002 34 3678
Fragments arranged by size:
3678 3002 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4640 5850 4640
Size: 1210 5538
BclI T'GATC_A
Cuts at: 1548 3111 1548
Size: 1563 5185
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2988 3422 3561
Size: 107 122 71 631 605 1316 434 139
Cuts at: 3561 5359 136
Size: 1798 1525
Fragments arranged by size:
1798 1525 1316 631 605 434 139 122
107 71
BglII A'GATC_T
Cuts at: 6743 6743
Size: 6748
BlpI GC'TnA_GC
Cuts at: 3344 4165 3344
Size: 821 5927
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2180 2922 2984 3033 3557 3663 4809
Size: 316 74 742 62 49 524 106 1146
Cuts at: 4809 5965 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1146 742 524 316 106 74
62 49
BmgBI CAC'GTC
Cuts at: 1318 2737 3664 1318
Size: 1419 927 4402
Fragments arranged by size:
4402 1419 927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 4479 5437
Size: 416 53 83 399 24 729 2333 958
Cuts at: 5437 442
Size: 1753
Fragments arranged by size:
2333 1753 958 729 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3179 3206 3878 3941 5408 1239
Size: 1940 27 672 63 1467 2579
Fragments arranged by size:
2579 1940 1467 672 63 27
Bpu10I CC'TnA_GC
Cuts at: 2396 2970 2396
Size: 574 6174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3752 4873 5738 6036 2336
Size: 1416 1121 865 298 3048
Fragments arranged by size:
3048 1416 1121 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3010 3734 5411 882
Size: 617 1511 724 1677 2219
Fragments arranged by size:
2219 1677 1511 724 617
BsaAI yAC'GTr
Cuts at: 493 1204 2806 3915 4539 493
Size: 711 1602 1109 624 2702
Fragments arranged by size:
2702 1602 1109 711 624
BsaBI GATnn'nnATC
Cuts at: 4001 6742 4001
Size: 2741 4007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 2592 2838 3472
Size: 53 83 186 936 960 99 246 634
Cuts at: 3472 3963 4555 4937 5572 275
Size: 491 592 382 635 1451
Fragments arranged by size:
1451 960 936 635 634 592 491 382
246 186 99 83 53
BsaWI w'CCGG_w
Cuts at: 2274 3309 5181 5579 5853 6000 6411 2274
Size: 1035 1872 398 274 147 411 2611
Fragments arranged by size:
2611 1872 1035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2783 2813 3033 3063 3665
Size: 30 165 30 1380 30 220 30 602
Cuts at: 3665 3695 3851 3881 1178
Size: 30 156 30 4045
Fragments arranged by size:
4045 1380 602 220 165 156 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2739 3176 3775 3836 1186
Size: 904 649 437 599 61 4098
Fragments arranged by size:
4098 904 649 599 437 61
BseYI C'CCAG_C
Cuts at: 2841 4035 4039 5951 6447 2841
Size: 1194 4 1912 496 3142
Fragments arranged by size:
3142 1912 1194 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2984 3629 1163
Size: 573 1248 645 4282
Fragments arranged by size:
4282 1248 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4198 4959 5987 1540
Size: 275 186 2197 761 1028 2301
Fragments arranged by size:
2301 2197 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2382 2523 3707 4809 4894 5965 2106
Size: 276 141 1184 1102 85 1071 2889
Fragments arranged by size:
2889 1184 1102 1071 276 141 85
BsmI GAATG_Cn'
Cuts at: 4259 4352 4259
Size: 93 6655
BsmBI CGTCTCn'nnnn_
Cuts at: 3182 3670 3818 3182
Size: 488 148 6112
Fragments arranged by size:
6112 488 148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2180 2382 2523 2922 2984 3033
Size: 399 316 74 202 141 399 62 49
Cuts at: 3033 3536 3557 3629 3663 3678 3707 4067 4809
Size: 503 21 72 34 15 29 360 742
Cuts at: 4809 4894 5965 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 503 399 399 360 316
202 141 85 74 72 62 49 34
29 21 15
BspEI T'CCGG_A
Cuts at: 3309 6411 3309
Size: 3102 3646
BspHI T'CATG_A
Cuts at: 4637 6367 4637
Size: 1730 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 4103 844
Size: 1547 1712 3489
Fragments arranged by size:
3489 1712 1547
BsrBI CCG'CTC
Cuts at: 1394 1823 3166 3737 3783 4635 1394
Size: 429 1343 571 46 852 3507
Fragments arranged by size:
3507 1343 852 571 429 46
BsrDI GCAATG_nn'
Cuts at: 65 1248 3434 5243 5417 65
Size: 1183 2186 1809 174 1396
Fragments arranged by size:
2186 1809 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3965 5392 5579 1700
Size: 110 2155 1427 187 2869
Fragments arranged by size:
2869 2155 1427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6748
BssSI C'ACGA_G
Cuts at: 2105 3538 4808 5820 2105
Size: 1433 1270 1012 3033
Fragments arranged by size:
3033 1433 1270 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4030 794
Size: 3236 3512
BstBI TT'CG_AA
Cuts at: 5552 5552
Size: 6748
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6748
BstXI CCAn_nnnn'nTGG
Cuts at: 3817 3817
Size: 6748
BstYI r'GATC_y
Cuts at: 1972 4002 4078 4445 4830 4847 6299 6385 6397
Size: 2030 76 367 385 17 1452 86 12
Cuts at: 6397 6743 1972
Size: 346 1977
Fragments arranged by size:
2030 1977 1452 385 367 346 86 76
17 12
Bsu36I CC'TnA_GG
Cuts at: 3874 3874
Size: 6748
BtgI C'CryG_G
Cuts at: 513 1065 2743 3327 3336 3501 513
Size: 552 1678 584 9 165 3760
Fragments arranged by size:
3760 1678 584 552 165 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6009
Fragments arranged by size:
6009 683 56
BtsI GCAGTG_nn'
Cuts at: 2216 3012 3274 3487 4264 5058 5078 2216
Size: 796 262 213 777 794 20 3886
Fragments arranged by size:
3886 796 794 777 262 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6713
DraI TTT'AAA
Cuts at: 4052 4052
Size: 6748
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2564 5755 809
Size: 507 1248 3191 1802
Fragments arranged by size:
3191 1802 1248 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 2686 3414
Size: 54 1425 50 275 18 502 354 728
Cuts at: 3414 4195 5084 6713 8
Size: 781 889 1629 43
Fragments arranged by size:
1629 1425 889 781 728 502 354 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4195 1537
Size: 275 2383 4090
Fragments arranged by size:
4090 2383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 3501 3524 3611 4678 1353
Size: 1286 862 23 87 1067 3423
Fragments arranged by size:
3423 1286 1067 862 87 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5335 5709 5855 387
Size: 1064 138 3746 374 146 1280
Fragments arranged by size:
3746 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3705 3705
Size: 6748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2620 3179 3206 3878 3941 4811 5408
Size: 393 988 559 27 672 63 870 597
Cuts at: 5408 6195 6463 1239
Size: 787 268 1524
Fragments arranged by size:
1524 988 870 787 672 597 559 393
268 63 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2999 3416 2999
Size: 417 6331
EcoO109I rG'GnC_Cy
Cuts at: 2126 2980 2981 3077 3979 2126
Size: 854 1 96 902 4895
Fragments arranged by size:
4895 902 854 96 1
EcoRI G'AATT_C
Cuts at: 4057 4435 4057
Size: 378 6370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2676 2676
Size: 6748
FspI TGC'GCA
Cuts at: 5254 5254
Size: 6748
HaeII r_GCGC'y
Cuts at: 1678 3582 3767 5895 1678
Size: 1904 185 2128 2531
Fragments arranged by size:
2531 2128 1904 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3033 3065 3665 3677 3697
Size: 32 135 32 1461 32 600 12 20
Cuts at: 3697 3709 3851 3883 5389 5421 5463 5495 1373
Size: 12 142 32 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 1461 600 142 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4092 4338 669
Size: 988 281 2154 246 3079
Fragments arranged by size:
3079 2154 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2606 748
Size: 1858 4890
HpaI GTT'AAC
Cuts at: 4338 4338
Size: 6748
KpnI G_GTAC'C
Cuts at: 4073 4073
Size: 6748
MfeI C'AATT_G
Cuts at: 4347 4347
Size: 6748
MluI A'CGCG_T
Cuts at: 6624 6624
Size: 6748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2961 3663 5862 6046 1305
Size: 1656 702 2199 184 2007
Fragments arranged by size:
2199 2007 1656 702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2688 3416 10
Size: 54 1425 343 856 728 3342
Fragments arranged by size:
3342 1425 856 728 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4706 5065 5224 518
Size: 648 3540 359 159 2042
Fragments arranged by size:
3540 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2854 3062 3384 3657 4009 4039 4164 4841
Size: 1248 208 322 273 352 30 125 677
Cuts at: 4841 5989 6234 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1248 1148 677 352 322 273 245
208 125 30
NaeI GCC'GGC
Cuts at: 3967 3967
Size: 6748
NcoI C'CATG_G
Cuts at: 513 1065 3327 3336 513
Size: 552 2262 9 3925
Fragments arranged by size:
3925 2262 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6748
NgoMIV G'CCGG_C
Cuts at: 3965 3965
Size: 6748
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6748
NotI GC'GGCC_GC
Cuts at: 4195 4195
Size: 6748
NspI r_CATG'y
Cuts at: 1132 1229 2567 2951 4033 4106 5651 1132
Size: 97 1338 384 1082 73 1545 2229
Fragments arranged by size:
2229 1545 1338 1082 384 97 73
PasI CC'CwG_GG
Cuts at: 1348 3524 1348
Size: 2176 4572
PciI A'CATG_T
Cuts at: 5647 5647
Size: 6748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3328 1108
Size: 2220 4528
PfoI T'CCnGG_A
Cuts at: 2895 3051 3087 3183 2895
Size: 156 36 96 6460
Fragments arranged by size:
6460 156 96 36
PmeI GTTT'AAAC
Cuts at: 4052 4052
Size: 6748
PmlI CAC'GTG
Cuts at: 2806 3915 2806
Size: 1109 5639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4817 4849 6330 6362 4817
Size: 32 1481 32 5203
Fragments arranged by size:
5203 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 3979 2126
Size: 1853 4895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6748
PsiI TTA'TAA
Cuts at: 4318 4318
Size: 6748
PspOMI G'GGCC_C
Cuts at: 2980 3553 2980
Size: 573 6175
PspXI vC'TCGA_Gb
Cuts at: 2246 2246
Size: 6748
PstI C_TGCA'G
Cuts at: 830 2223 2706 4100 830
Size: 1393 483 1394 3478
Fragments arranged by size:
3478 1394 1393 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6748
PvuII CAG'CTG
Cuts at: 4009 4039 4009
Size: 30 6718
SacI G_AGCT'C
Cuts at: 3707 3707
Size: 6748
SalI G'TCGA_C
Cuts at: 1936 4090 1936
Size: 2154 4594
SanDI GG'GwC_CC
Cuts at: 3979 3979
Size: 6748
SapI GCTCTTCn'nnn_
Cuts at: 3524 3524
Size: 6748
SbfI CC_TGCA'GG
Cuts at: 4100 4100
Size: 6748
ScaI AGT'ACT
Cuts at: 4996 6708 4996
Size: 1712 5036
SfcI C'TryA_G
Cuts at: 826 1045 2219 2702 3907 4096 5231 5912 6103
Size: 219 1174 483 1205 189 1135 681 191
Cuts at: 6103 826
Size: 1471
Fragments arranged by size:
1471 1205 1174 1135 681 483 219 191
189
SfiI GGCCn_nnn'nGGCC
Cuts at: 2988 2988
Size: 6748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6748
SmaI CCC'GGG
Cuts at: 1872 4075 1872
Size: 2203 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 2351 3767 4852 5753 6015
Size: 197 926 303 105 1416 1085 901 262
Cuts at: 6015 820
Size: 1553
Fragments arranged by size:
1553 1416 1085 926 901 303 262 197
105
SnaBI TAC'GTA
Cuts at: 493 4539 493
Size: 4046 2702
SpeI A'CTAG_T
Cuts at: 152 3941 152
Size: 3789 2959
SphI G_CATG'C
Cuts at: 1132 2951 4106 1132
Size: 1819 1155 3774
Fragments arranged by size:
3774 1819 1155
SspI AAT'ATT
Cuts at: 5 52 4672 5
Size: 47 4620 2081
Fragments arranged by size:
4620 2081 47
StuI AGG'CCT
Cuts at: 1517 2730 3855 1517
Size: 1213 1125 4410
Fragments arranged by size:
4410 1213 1125
StyI C'CwwG_G
Cuts at: 513 1065 2235 2529 3327 3336 3417 4187 513
Size: 552 1170 294 798 9 81 770 3074
Fragments arranged by size:
3074 1170 798 770 552 294 81 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2774 4775 4792 4945 5130 5575 325
Size: 1255 1194 2001 17 153 185 445 1498
Fragments arranged by size:
2001 1498 1255 1194 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 4994 6706
Size: 274 80 33 51 1443 792 2225 1712
Cuts at: 6706 96
Size: 138
Fragments arranged by size:
2225 1712 1443 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 3504 3536 3971 4003 6708 6740 3206
Size: 32 266 32 435 32 2705 32 3214
Fragments arranged by size:
3214 2705 435 266 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3929 1316
Size: 2613 4135
XbaI T'CTAG_A
Cuts at: 4084 4084
Size: 6748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3232 3334 3232
Size: 102 6646
XhoI C'TCGA_G
Cuts at: 1943 2246 1943
Size: 303 6445
XmaI C'CCGG_G
Cuts at: 1870 4073 1870
Size: 2203 4545
XmnI GAAnn'nnTTC
Cuts at: 4877 4877
Size: 6748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2494 4556 276
Size: 53 83 186 936 960 2062 2468
Fragments arranged by size:
2468 2062 960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PsrI RsrII SacII SexAI SfoI SrfI
SwaI