Restriction map for
pFN21AA0471
(Circular) (Six-base) MAPSORT of: pFN21AA0471.seq Check: 1943 from: 1 to: 5815
pFN21AA0471 5815 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2592 2608 3878 5262 5530 1632
Size: 707 253 16 1270 1384 268 1917
Fragments arranged by size:
1917 1384 1270 707 268 253 16
AflII C'TTAA_G
Cuts at: 820 1017 2885 820
Size: 197 1868 3750
Fragments arranged by size:
3750 1868 197
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 3085 4646 3085
Size: 1561 4254
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 2793 1166
Size: 1627 4188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2560 3558 5130 2560
Size: 998 1572 3245
Fragments arranged by size:
3245 1572 998
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2958 3124 3350 3502 5611 1493
Size: 1465 166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1465 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 3062 3136 4591 618
Size: 325 217 627 230 1045 74 1455 1842
Fragments arranged by size:
1842 1455 1045 627 325 230 217 74
BanII G_rGCy'C
Cuts at: 1391 2541 2989 3134 1391
Size: 1150 448 145 4072
Fragments arranged by size:
4072 1150 448 145
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2190 2278 2379 2517 2614 2669 928
Size: 971 291 88 101 138 97 55 4074
Fragments arranged by size:
4074 971 291 138 101 97 88 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2694 5810 2694
Size: 3116 2699
BlpI GC'TnA_GC
Cuts at: 2040 3232 2040
Size: 1192 4623
Bme1580I G_kGCm'C
Cuts at: 1790 2022 3065 3876 5032 1790
Size: 232 1043 811 1156 2573
Fragments arranged by size:
2573 1156 1043 811 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 4475 1239
Size: 1502 1734 2579
Fragments arranged by size:
2579 1734 1502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3085 4248 4646 4920 5067 5478 3085
Size: 1163 398 274 147 411 3422
Fragments arranged by size:
3422 1163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 1178
Size: 30 165 30 1533 30 4027
Fragments arranged by size:
4027 1533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2591 2762 2997 1186
Size: 915 490 171 235 4004
Fragments arranged by size:
4004 915 490 235 171
BseYI C'CCAG_C
Cuts at: 2026 2032 2607 3066 3093 5018 5514 2026
Size: 6 575 459 27 1925 496 2327
Fragments arranged by size:
2327 1925 575 496 459 27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3113 3876 3961 5032 2541
Size: 572 763 85 1071 3324
Fragments arranged by size:
3324 1071 763 572 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2541 2989 3065 3113 3134 3876
Size: 399 232 519 448 76 48 21 742
Cuts at: 3876 3961 5032 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 448 399 232 85
76 48 21
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 844
Size: 2326 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 3071 4310 4484 65
Size: 1183 1428 395 1239 174 1396
Fragments arranged by size:
1428 1396 1239 1183 395 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3085 4459 4646 1700
Size: 110 1275 1374 187 2869
Fragments arranged by size:
2869 1374 1275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2790 794
Size: 1996 3819
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 3088 1184
Size: 1904 3911
BstYI r'GATC_y
Cuts at: 1972 2694 3145 3512 3897 3914 5366 5452 5464
Size: 722 451 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 722 451 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3057 513
Size: 552 939 1053 3271
Fragments arranged by size:
3271 1053 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 2800 3331 4125 4145 2788
Size: 12 531 794 20 4458
Fragments arranged by size:
4458 794 531 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 2217 3119 2217
Size: 902 4913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3054 3262 4151
Size: 54 1425 50 275 18 1224 208 889
Cuts at: 4151 5780 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1224 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2568 2576 2592 3745 1353
Size: 1215 8 16 1153 3423
Fragments arranged by size:
3423 1215 1153 16 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 5815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2592 2608 2741 3878 4475 5262
Size: 393 707 253 16 133 1137 597 787
Cuts at: 5262 5530 1239
Size: 268 1524
Fragments arranged by size:
1524 1137 787 707 597 393 268 253
133 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 5815
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 5815
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 4456 4488 4530
Size: 32 135 32 556 32 2296 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 2296 556 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2412 4929 5113 1305
Size: 1107 2517 184 2007
Fragments arranged by size:
2517 2007 1107 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2793 3773 4132 4291 518
Size: 648 1627 980 359 159 2042
Fragments arranged by size:
2042 1627 980 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2362 2896 3231 3908 5056 5301 1606
Size: 430 326 534 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 430 335 326 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2077 2784 3173 4718 1132
Size: 97 848 707 389 1545 2229
Fragments arranged by size:
2229 1545 848 707 389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2196 2795 2937 3167 830
Size: 1366 599 142 230 3478
Fragments arranged by size:
3478 1366 599 230 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2036 2362 2896 2036
Size: 326 534 4955
Fragments arranged by size:
4955 534 326
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2192 2667 2791 2933 3163 4298 4979
Size: 219 1147 475 124 142 230 1135 681
Cuts at: 4979 5170 826
Size: 191 1471
Fragments arranged by size:
1471 1147 1135 681 475 230 219 191
142 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 3919 4820 5082 820
Size: 197 926 942 1034 901 262 1553
Fragments arranged by size:
1553 1034 942 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 2464 152
Size: 2312 3503
SphI G_CATG'C
Cuts at: 1132 2784 3173 1132
Size: 1652 389 3774
Fragments arranged by size:
3774 1652 389
SspI AAT'ATT
Cuts at: 5 52 2296 3739 5
Size: 47 2244 1443 2081
Fragments arranged by size:
2244 2081 1443 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 3102 3254 513
Size: 552 939 1098 152 3074
Fragments arranged by size:
3074 1098 939 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3080 3107 3115 3842 3859 4012 4197
Size: 1255 1500 27 8 727 17 153 185
Cuts at: 4197 4642 325
Size: 445 1498
Fragments arranged by size:
1500 1498 1255 727 445 185 153 27
17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 5775 5807 2936
Size: 32 2807 32 2944
Fragments arranged by size:
2944 2807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2557 2935 2428
Size: 129 378 5308
Fragments arranged by size:
5308 378 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 2187 3944 2187
Size: 1757 4058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI