Restriction map for
pFN21AA0460
(Circular) (Six-base) MAPSORT of: pFN21AA0460.seq Check: 3730 from: 1 to: 8977
pFN21AA0460 8977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2662 3388 3394 2662
Size: 726 6 8245
Fragments arranged by size:
8245 726 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6787 278
Size: 53 83 186 936 5251 2468
Fragments arranged by size:
5251 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2289 4907 6320 1937
Size: 352 2618 1413 4594
Fragments arranged by size:
4594 2618 1413 352
Acc65I G'GTAC_C
Cuts at: 6298 6298
Size: 8977
AclI AA'CG_TT
Cuts at: 1563 7104 7477 1563
Size: 5541 373 3063
Fragments arranged by size:
5541 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2540 2862 3506 3561 3690 3711 3831
Size: 597 311 322 644 55 129 21 120
Cuts at: 3831 3846 4624 7040 8424 8692 1632
Size: 15 778 2416 1384 268 1917
Fragments arranged by size:
2416 1917 1384 778 644 597 322 311
268 129 120 55 21 15
AfeI AGC'GCT
Cuts at: 2498 2498
Size: 8977
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8780
AflIII A'CryG_T
Cuts at: 1203 7876 8853 1203
Size: 6673 977 1327
Fragments arranged by size:
6673 1327 977
AgeI A'CCGG_T
Cuts at: 4671 7808 4671
Size: 3137 5840
AhdI GACnn_n'nnGTC
Cuts at: 7706 7706
Size: 8977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8977
AlwNI CAG_nnn'CTG
Cuts at: 2840 3152 3494 5852 6720 8292 2840
Size: 312 342 2358 868 1572 3525
Fragments arranged by size:
3525 2358 1572 868 342 312
ApaI G_GGCC'C
Cuts at: 4579 5143 5948 6035 6233 4579
Size: 564 805 87 198 7323
Fragments arranged by size:
7323 805 564 198 87
ApaLI G'TGCA_C
Cuts at: 7034 8190 7034
Size: 1156 7821
ApoI r'AATT_y
Cuts at: 1493 2453 2618 2648 2718 4538 4916 5685 6286
Size: 960 165 30 70 1820 378 769 601
Cuts at: 6286 6512 6664 8773 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 960 769 601 378 226
165 152 70 30
AseI AT'TA_AT
Cuts at: 160 1032 7531 160
Size: 872 6499 1606
Fragments arranged by size:
6499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
AvaI C'yCGr_G
Cuts at: 1870 1943 4230 5577 6302 1870
Size: 73 2287 1347 725 4545
Fragments arranged by size:
4545 2287 1347 725 73
AvrII C'CTAG_G
Cuts at: 6161 6161
Size: 8977
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3081 3114 3081
Size: 33 8944
BamHI G'GATC_C
Cuts at: 2230 6307 6674 2230
Size: 4077 367 4533
Fragments arranged by size:
4533 4077 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4634 5404 6298 7753 618
Size: 325 217 627 2847 770 894 1455 1842
Fragments arranged by size:
2847 1842 1455 894 770 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3744 4239 4534 4579 5143 5948 6035 6233
Size: 2353 495 295 45 564 805 87 198
Cuts at: 6233 6296 1391
Size: 63 4072
Fragments arranged by size:
4072 2353 805 564 495 295 198 87
63 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3006 928
Size: 971 1107 6899
Fragments arranged by size:
6899 1107 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 7168 7202 1903
Size: 34 2087 34 3110 34 3678
Fragments arranged by size:
3678 3110 2087 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3097 6869 8079 3097
Size: 3772 1210 3995
Fragments arranged by size:
3995 3772 1210
BclI T'GATC_A
Cuts at: 1548 5050 1548
Size: 3502 5475
BfrBI ATG'CAT
Cuts at: 2765 2765
Size: 8977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3716 5373 5524
Size: 107 122 71 631 605 2044 1657 151
Cuts at: 5524 7588 136
Size: 2064 1525
Fragments arranged by size:
2064 2044 1657 1525 631 605 151 122
107 71
BglII A'GATC_T
Cuts at: 2096 8972 2096
Size: 6876 2101
BlpI GC'TnA_GC
Cuts at: 5904 6394 5904
Size: 490 8487
Bme1580I G_kGCm'C
Cuts at: 1790 3640 4579 4994 5143 5407 5948 6035 6233
Size: 1850 939 415 149 264 541 87 198
Cuts at: 6233 7038 8194 1790
Size: 805 1156 2573
Fragments arranged by size:
2573 1850 1156 939 805 541 415 264
198 149 87
BmgBI CAC'GTC
Cuts at: 1318 4863 4905 1318
Size: 3545 42 5390
Fragments arranged by size:
5390 3545 42
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 3140 3312
Size: 416 53 83 399 24 1509 214 172
Cuts at: 3312 3536 3543 4758 4790 6708 7666 442
Size: 224 7 1215 32 1918 958 1753
Fragments arranged by size:
1918 1753 1509 1215 958 416 399 224
214 172 83 53 32 24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 3901 6014 2650
Size: 1251 2113 5613
Fragments arranged by size:
5613 2113 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3315 3616 3890 5691 5820 7637 1239
Size: 2076 301 274 1801 129 1817 2579
Fragments arranged by size:
2579 2076 1817 1801 301 274 129
Bpu10I CC'TnA_GC
Cuts at: 3272 3968 5978 3272
Size: 696 2010 6271
Fragments arranged by size:
6271 2010 696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 7102 7967 8265 2417
Size: 4685 865 298 3129
Fragments arranged by size:
4685 3129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3859 4312 6177 6199 7640 882
Size: 617 2360 453 1865 22 1441 2219
Fragments arranged by size:
2360 2219 1865 1441 617 453 22
BsaAI yAC'GTr
Cuts at: 493 1204 4946 6768 493
Size: 711 3742 1822 2702
Fragments arranged by size:
3742 2702 1822 711
BsaBI GATnn'nnATC
Cuts at: 8971 8971
Size: 8977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6784 7166 7801 275
Size: 53 83 186 936 5251 382 635 1451
Fragments arranged by size:
5251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2091 4671 7410 7808 8082 8229 8640 2091
Size: 2580 2739 398 274 147 411 2428
Fragments arranged by size:
2739 2580 2428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 3154 3184 3863
Size: 30 165 30 1461 30 260 30 679
Cuts at: 3863 3893 5689 5719 1178
Size: 30 1796 30 4436
Fragments arranged by size:
4436 1796 1461 679 260 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4779 5086 5089 5655 5697 5700 5766 6060
Size: 3593 307 3 566 42 3 66 294
Cuts at: 6060 6063 1186
Size: 3 4100
Fragments arranged by size:
4100 3593 566 307 294 66 42 3
3 3
BseYI C'CCAG_C
Cuts at: 3214 3350 4184 4982 5033 5815 8180 8676 3214
Size: 136 834 798 51 782 2365 496 3515
Fragments arranged by size:
3515 2365 834 798 782 496 136 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2578 3366 3447 3480 1163
Size: 573 842 788 81 33 6660
Fragments arranged by size:
6660 842 788 573 81 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6427 7188 8216 1540
Size: 275 186 4426 761 1028 2301
Fragments arranged by size:
4426 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3575 4239 5073 5291 5679 5838 7038 7123 8194
Size: 664 834 218 388 159 1200 85 1071
Cuts at: 8194 3575
Size: 4358
Fragments arranged by size:
4358 1200 1071 834 664 388 218 159
85
BsmI GAATG_Cn'
Cuts at: 3453 6488 6581 3453
Size: 3035 93 5849
Fragments arranged by size:
5849 3035 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4613 5098 4613
Size: 485 8492
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3575 3640 3744 4239 4534 4579 4994
Size: 399 1785 65 104 495 295 45 415
Cuts at: 4994 5073 5143 5291 5407 5679 5838 5948 6035
Size: 79 70 148 116 272 159 110 87
Cuts at: 6035 6233 6296 7038 7123 8194 1391
Size: 198 63 742 85 1071 2174
Fragments arranged by size:
2174 1785 1071 742 495 415 399 295
272 198 159 148 116 110 104 87
85 79 70 65 63 45
BspEI T'CCGG_A
Cuts at: 2091 8640 2091
Size: 6549 2428
BspHI T'CATG_A
Cuts at: 3291 6866 8596 3291
Size: 3575 1730 3672
Fragments arranged by size:
3672 3575 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2662 3076 3388 3394 6332 844
Size: 1818 414 312 6 2938 3489
Fragments arranged by size:
3489 2938 1818 414 312 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4841 6864 1394
Size: 429 3018 2023 3507
Fragments arranged by size:
3507 3018 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7472 7646 65
Size: 1183 6224 174 1396
Fragments arranged by size:
6224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4671 7621 7808 1700
Size: 110 2861 2950 187 2869
Fragments arranged by size:
2950 2869 2861 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8977
BssSI C'ACGA_G
Cuts at: 7037 8049 7037
Size: 1012 7965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8977
BstBI TT'CG_AA
Cuts at: 5615 7781 5615
Size: 2166 6811
BstEII G'GTnAC_C
Cuts at: 1184 4674 4850 1184
Size: 3490 176 5311
Fragments arranged by size:
5311 3490 176
BstXI CCAn_nnnn'nTGG
Cuts at: 3778 4541 3778
Size: 763 8214
BstYI r'GATC_y
Cuts at: 1972 2096 2230 2836 3247 5608 6307 6674 7059
Size: 124 134 606 411 2361 699 367 385
Cuts at: 7059 7076 8528 8614 8626 8972 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2361 1977 1452 699 606 411 385 367
346 134 124 86 17 12
BstZ17I GTA'TAC
Cuts at: 2290 2290
Size: 8977
Bsu36I CC'TnA_GG
Cuts at: 5553 5553
Size: 8977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2943 4845 5938 6040 513
Size: 552 939 939 1902 1093 102 3450
Fragments arranged by size:
3450 1902 1093 939 939 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8238
Fragments arranged by size:
8238 683 56
BtsI GCAGTG_nn'
Cuts at: 2396 3497 4135 4285 5743 6082 6493 7287 7307
Size: 1101 638 150 1458 339 411 794 20
Cuts at: 7307 2396
Size: 4066
Fragments arranged by size:
4066 1458 1101 794 638 411 339 150
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8942
DraI TTT'AAA
Cuts at: 2266 2799 3760 5380 6201 6281 2266
Size: 533 961 1620 821 80 4962
Fragments arranged by size:
4962 1620 961 821 533 80
DraIII CAC_nnn'GTG
Cuts at: 5842 5842
Size: 8977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4269 5212 7984 809
Size: 507 2953 943 2772 1802
Fragments arranged by size:
2953 2772 1802 943 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4972 6146 6424
Size: 54 1425 50 275 18 3142 1174 278
Cuts at: 6424 7313 8942 8
Size: 889 1629 43
Fragments arranged by size:
3142 1629 1425 1174 889 278 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6424 1537
Size: 275 4612 4090
Fragments arranged by size:
4612 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2472 2619 3139 3183 3703 4345 5061 6907
Size: 1119 147 520 44 520 642 716 1846
Cuts at: 6907 1353
Size: 3423
Fragments arranged by size:
3423 1846 1119 716 642 520 520 147
44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7564 7938 8084 387
Size: 1064 138 5975 374 146 1280
Fragments arranged by size:
5975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4237 4237
Size: 8977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2540 2862 3315 3506 3561 3616
Size: 393 597 311 322 453 191 55 55
Cuts at: 3616 3690 3711 3831 3846 3890 4624 5691 5820
Size: 74 21 120 15 44 734 1067 129
Cuts at: 5820 7040 7637 8424 8692 1239
Size: 1220 597 787 268 1524
Fragments arranged by size:
1524 1220 1067 787 734 597 597 453
393 322 311 268 191 129 120 74
55 55 44 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3330 3428 3330
Size: 98 8879
EcoO109I rG'GnC_Cy
Cuts at: 2467 4412 4575 4576 5139 5140 5895 5927 5944
Size: 1945 163 1 563 1 755 32 17
Cuts at: 5944 5945 5959 6092 6229 6230 2467
Size: 1 14 133 137 1 5214
Fragments arranged by size:
5214 1945 755 563 163 137 133 32
17 14 1 1 1 1
EcoRI G'AATT_C
Cuts at: 5685 6286 6664 5685
Size: 601 378 7998
Fragments arranged by size:
7998 601 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3771 4573 3771
Size: 802 8175
FspI TGC'GCA
Cuts at: 7483 7483
Size: 8977
HaeII r_GCGC'y
Cuts at: 1678 2500 3990 8124 1678
Size: 822 1490 4134 2531
Fragments arranged by size:
4134 2531 1490 822
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2896 2971 3003 3017
Size: 32 135 32 1292 32 75 32 14
Cuts at: 3017 3049 3154 3186 3385 3417 3863 3895 4127
Size: 32 105 32 199 32 446 32 232
Cuts at: 4127 4159 4307 4339 4595 4627 5324 5356 6096
Size: 32 148 32 256 32 697 32 740
Cuts at: 6096 6128 7618 7650 7692 7724 1373
Size: 32 1490 32 42 32 2626
Fragments arranged by size:
2626 1490 1292 740 697 446 256 232
199 148 135 105 75 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2538 2826 4908 5791 6321 6567
Size: 988 281 600 288 2082 883 530 246
Cuts at: 6567 669
Size: 3079
Fragments arranged by size:
3079 2082 988 883 600 530 288 281
246
HindIII A'AGCT_T
Cuts at: 748 2322 3658 748
Size: 1574 1336 6067
Fragments arranged by size:
6067 1574 1336
HpaI GTT'AAC
Cuts at: 6567 6567
Size: 8977
KpnI G_GTAC'C
Cuts at: 6302 6302
Size: 8977
MfeI C'AATT_G
Cuts at: 2816 3723 6576 2816
Size: 907 2853 5217
Fragments arranged by size:
5217 2853 907
MluI A'CGCG_T
Cuts at: 8853 8853
Size: 8977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 3795 4743 5466 8091 8275 1305
Size: 1485 1005 948 723 2625 184 2007
Fragments arranged by size:
2625 2007 1485 1005 948 723 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4974 10
Size: 54 1425 343 3142 4013
Fragments arranged by size:
4013 3142 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5253 6935 7294 7453 518
Size: 648 4087 1682 359 159 2042
Fragments arranged by size:
4087 2042 1682 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2356 3083 5361 6393 7070 8218 8463
Size: 610 140 727 2278 1032 677 1148 245
Cuts at: 8463 1606
Size: 2120
Fragments arranged by size:
2278 2120 1148 1032 727 677 610 245
140
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 6040 513
Size: 552 939 939 3097 3450
Fragments arranged by size:
3450 3097 939 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8977
NotI GC'GGCC_GC
Cuts at: 6424 6424
Size: 8977
NsiI A_TGCA'T
Cuts at: 2767 2767
Size: 8977
NspI r_CATG'y
Cuts at: 1132 1229 6335 7880 1132
Size: 97 5106 1545 2229
Fragments arranged by size:
5106 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3737 4208 4622 5931 1348
Size: 2389 471 414 1309 4394
Fragments arranged by size:
4394 2389 1309 471 414
PciI A'CATG_T
Cuts at: 7876 7876
Size: 8977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4208 5003 6019 6041 1108
Size: 3100 795 1016 22 4044
Fragments arranged by size:
4044 3100 1016 795 22
PfoI T'CCnGG_A
Cuts at: 3331 4581 5668 3331
Size: 1250 1087 6640
Fragments arranged by size:
6640 1250 1087
PmeI GTTT'AAAC
Cuts at: 6281 6281
Size: 8977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6159 6191 7046 7078 8559 8591 6159
Size: 32 855 32 1481 32 6545
Fragments arranged by size:
6545 1481 855 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5895 5927 5959 6092 5895
Size: 32 32 133 8780
Fragments arranged by size:
8780 133 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8977
PsiI TTA'TAA
Cuts at: 6547 6547
Size: 8977
PspOMI G'GGCC_C
Cuts at: 4575 5139 5944 6031 6229 4575
Size: 564 805 87 198 7323
Fragments arranged by size:
7323 805 564 198 87
PstI C_TGCA'G
Cuts at: 830 3073 6329 830
Size: 2243 3256 3478
Fragments arranged by size:
3478 3256 2243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8977
PvuII CAG'CTG
Cuts at: 2216 2356 3083 2216
Size: 140 727 8110
Fragments arranged by size:
8110 727 140
SacI G_AGCT'C
Cuts at: 4239 4239
Size: 8977
SalI G'TCGA_C
Cuts at: 1936 4906 6319 1936
Size: 2970 1413 4594
Fragments arranged by size:
4594 2970 1413
SanDI GG'GwC_CC
Cuts at: 5895 6092 5895
Size: 197 8780
SapI GCTCTTCn'nnn_
Cuts at: 2472 5061 2472
Size: 2589 6388
SbfI CC_TGCA'GG
Cuts at: 6329 6329
Size: 8977
ScaI AGT'ACT
Cuts at: 2062 7225 8937 2062
Size: 5163 1712 2102
Fragments arranged by size:
5163 2102 1712
SfcI C'TryA_G
Cuts at: 826 1045 2250 3069 5145 6325 7460 8141 8332
Size: 219 1205 819 2076 1180 1135 681 191
Cuts at: 8332 826
Size: 1471
Fragments arranged by size:
2076 1471 1205 1180 1135 819 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8977
SmaI CCC'GGG
Cuts at: 1872 6304 1872
Size: 4432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 5577 7081 7982 8244 820
Size: 197 926 489 3145 1504 901 262 1553
Fragments arranged by size:
3145 1553 1504 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 6768 493
Size: 6275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8977
SphI G_CATG'C
Cuts at: 1132 6335 1132
Size: 5203 3774
SspI AAT'ATT
Cuts at: 5 52 6901 5
Size: 47 6849 2081
Fragments arranged by size:
6849 2081 47
StuI AGG'CCT
Cuts at: 1517 3131 3507 3719 5367 1517
Size: 1614 376 212 1648 5127
Fragments arranged by size:
5127 1648 1614 376 212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2943 3429 5154 5344 5487 5868
Size: 552 939 939 486 1725 190 143 381
Cuts at: 5868 6040 6087 6161 6416 513
Size: 172 47 74 255 3074
Fragments arranged by size:
3074 1725 939 939 552 486 381 255
190 172 143 74 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4105 4109 4198 4809 4869 4873 5042
Size: 1255 2525 4 89 611 60 4 169
Cuts at: 5042 5602 5966 7004 7021 7174 7359 7804 325
Size: 560 364 1038 17 153 185 445 1498
Fragments arranged by size:
2525 1498 1255 1038 611 560 445 364
185 169 153 89 60 17 4 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2060 2200 4190
Size: 274 80 33 51 1443 83 140 1990
Cuts at: 4190 7223 8935 96
Size: 3033 1712 138
Fragments arranged by size:
3033 1990 1712 1443 274 140 138 83
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5156 5188 8937 8969 5156
Size: 32 3749 32 5164
Fragments arranged by size:
5164 3749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3484 1316
Size: 2168 6809
XbaI T'CTAG_A
Cuts at: 6313 6313
Size: 8977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3865 4867 3865
Size: 1002 7975
XhoI C'TCGA_G
Cuts at: 1943 5577 1943
Size: 3634 5343
XmaI C'CCGG_G
Cuts at: 1870 6302 1870
Size: 4432 4545
XmnI GAAnn'nnTTC
Cuts at: 2606 5621 6171 7106 2606
Size: 3015 550 935 4477
Fragments arranged by size:
4477 3015 935 550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6785 276
Size: 53 83 186 936 5251 2468
Fragments arranged by size:
5251 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BbvCI BsiWI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI