Restriction map for
pFN21AA0447
(Circular) (Six-base) MAPSORT of: pFN21AA0447.seq Check: 4168 from: 1 to: 5920
pFN21AA0447 5920 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2844 3730 278
Size: 53 83 186 936 1308 886 2468
Fragments arranged by size:
2468 1308 936 886 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3263 1937
Size: 1326 4594
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 4047 4420 1563
Size: 2484 373 3063
Fragments arranged by size:
3063 2484 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 2857 2915 3983 5367 5635 1632
Size: 610 615 58 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1068 615 610 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 4819 5796 1203
Size: 3616 977 1327
Fragments arranged by size:
3616 1327 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 2112 1166
Size: 946 4974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 5888
AlwNI CAG_nnn'CTG
Cuts at: 2265 2341 3145 3158 3663 5235 2265
Size: 76 804 13 505 1572 2950
Fragments arranged by size:
2950 1572 804 505 76 13
ApaI G_GGCC'C
Cuts at: 3169 3169
Size: 5920
ApaLI G'TGCA_C
Cuts at: 2641 3977 5133 2641
Size: 1336 1156 3428
Fragments arranged by size:
3428 1336 1156
ApoI r'AATT_y
Cuts at: 1493 3229 3455 3607 5716 1493
Size: 1736 226 152 2109 1697
Fragments arranged by size:
2109 1736 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4474 160
Size: 872 3442 1606
Fragments arranged by size:
3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 2823 3245 1870
Size: 73 880 422 4545
Fragments arranged by size:
4545 880 422 73
BamHI G'GATC_C
Cuts at: 3250 3617 3250
Size: 367 5553
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3241 4696 618
Size: 325 217 627 1454 1455 1842
Fragments arranged by size:
1842 1455 1454 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2222 2316 2537 3169 3239 1391
Size: 705 126 94 221 632 70 4072
Fragments arranged by size:
4072 705 632 221 126 94 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4111 4145 1903
Size: 34 2174 34 3678
Fragments arranged by size:
3678 2174 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 2556 1548
Size: 1008 4912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4531 136
Size: 107 122 71 631 605 2859 1525
Fragments arranged by size:
2859 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5915 5915
Size: 5920
BlpI GC'TnA_GC
Cuts at: 2741 3023 3337 2741
Size: 282 314 5324
Fragments arranged by size:
5324 314 282
Bme1580I G_kGCm'C
Cuts at: 1790 2645 3169 3981 5137 1790
Size: 855 524 812 1156 2573
Fragments arranged by size:
2573 1156 855 812 524
BmgBI CAC'GTC
Cuts at: 1318 2890 1318
Size: 1572 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2612 2840 3651
Size: 416 53 83 399 24 1195 228 811
Cuts at: 3651 4609 442
Size: 958 1753
Fragments arranged by size:
1753 1195 958 811 416 399 228 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4580 1239
Size: 3341 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4045 4910 5208 4045
Size: 865 298 4757
Fragments arranged by size:
4757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 4583 882
Size: 617 678 2406 2219
Fragments arranged by size:
2406 2219 678 617
BsaAI yAC'GTr
Cuts at: 493 1204 3711 493
Size: 711 2507 2702
Fragments arranged by size:
2702 2507 711
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2841 3727 4109 4744
Size: 53 83 186 936 1308 886 382 635
Cuts at: 4744 275
Size: 1451
Fragments arranged by size:
1451 1308 936 886 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4353 4751 5025 5172 5583 4353
Size: 398 274 147 411 4690
Fragments arranged by size:
4690 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 1178
Size: 30 165 30 1362 30 4303
Fragments arranged by size:
4303 1362 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2872 1186
Size: 1686 4234
BseYI C'CCAG_C
Cuts at: 2026 2088 2302 2315 5123 5619 2026
Size: 62 214 13 2808 496 2327
Fragments arranged by size:
2808 2327 496 214 62 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2251 2354 1163
Size: 573 515 103 4729
Fragments arranged by size:
4729 573 515 103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2685 3066 3370 4131 5159 1540
Size: 275 186 684 381 304 761 1028 2301
Fragments arranged by size:
2301 1028 761 684 381 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2222 2537 2645 3981 4066 5137 2222
Size: 315 108 1336 85 1071 3005
Fragments arranged by size:
3005 1336 1071 315 108 85
BsiWI C'GTAC_G
Cuts at: 2946 2946
Size: 5920
BsmI GAATG_Cn'
Cuts at: 3431 3524 3431
Size: 93 5827
BsmBI CGTCTCn'nnnn_
Cuts at: 2516 2516
Size: 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2222 2316 2537 2645 3169 3239
Size: 399 306 126 94 221 108 524 70
Cuts at: 3239 3981 4066 5137 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 399 306 221 126
108 94 85 70
BspEI T'CCGG_A
Cuts at: 5583 5583
Size: 5920
BspHI T'CATG_A
Cuts at: 3809 5539 3809
Size: 1730 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 3275 844
Size: 1220 1211 3489
Fragments arranged by size:
3489 1220 1211
BsrBI CCG'CTC
Cuts at: 1394 1823 2862 3807 1394
Size: 429 1039 945 3507
Fragments arranged by size:
3507 1039 945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4415 4589 65
Size: 1183 3167 174 1396
Fragments arranged by size:
3167 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4564 4751 1700
Size: 110 2754 187 2869
Fragments arranged by size:
2869 2754 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 3980 4992 3980
Size: 1012 4908
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5920
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 2105 3082 1184
Size: 921 977 4022
Fragments arranged by size:
4022 977 921
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 2704
Size: 5920
BstYI r'GATC_y
Cuts at: 1972 3250 3617 4002 4019 5471 5557 5569 5915
Size: 1278 367 385 17 1452 86 12 346
Cuts at: 5915 1972
Size: 1977
Fragments arranged by size:
1977 1452 1278 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2187 2349 513
Size: 552 1122 162 4084
Fragments arranged by size:
4084 1122 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5181
Fragments arranged by size:
5181 683 56
BtsI GCAGTG_nn'
Cuts at: 3436 4230 4250 3436
Size: 794 20 5106
Fragments arranged by size:
5106 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3224 3224
Size: 5920
DraIII CAC_nnn'GTG
Cuts at: 2247 2247
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4927 809
Size: 507 3611 1802
Fragments arranged by size:
3611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2682 2805
Size: 54 1425 50 275 18 816 36 123
Cuts at: 2805 3063 3160 3367 4256 5885 8
Size: 258 97 207 889 1629 43
Fragments arranged by size:
1629 1425 889 816 275 258 207 123
97 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2682 3063 3367 1537
Size: 275 870 381 304 4090
Fragments arranged by size:
4090 870 381 304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2052 2279 2525 2861 3850 1353
Size: 678 21 227 246 336 989 3423
Fragments arranged by size:
3423 989 678 336 246 227 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2527 4507 4881 5027 387
Size: 1064 138 938 1980 374 146 1280
Fragments arranged by size:
1980 1280 1064 938 374 146 138
EcoICRI GAG'CTC
Cuts at: 2220 2535 2220
Size: 315 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2857 2915 3983 4580 5367 5635
Size: 393 610 615 58 1068 597 787 268
Cuts at: 5635 1239
Size: 1524
Fragments arranged by size:
1524 1068 787 615 610 597 393 268
58
EcoO109I rG'GnC_Cy
Cuts at: 3094 3166 3202 3094
Size: 72 36 5812
Fragments arranged by size:
5812 72 36
EcoRI G'AATT_C
Cuts at: 3229 3607 3229
Size: 378 5542
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 2231 2639 2796 3137 5067 1678
Size: 553 408 157 341 1930 2531
Fragments arranged by size:
2531 1930 553 408 341 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2548 2580 4561 4593 4635
Size: 32 135 32 976 32 1981 32 42
Cuts at: 4635 4667 1373
Size: 32 2626
Fragments arranged by size:
2626 1981 976 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2602 3264 3510 669
Size: 988 281 664 662 246 3079
Fragments arranged by size:
3079 988 664 662 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5034 5218 1305
Size: 3729 184 2007
Fragments arranged by size:
3729 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2807 10
Size: 54 1425 343 975 3123
Fragments arranged by size:
3123 1425 975 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 3878 4237 4396 518
Size: 648 946 1766 359 159 2042
Fragments arranged by size:
2042 1766 946 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2745 2798 3190 3336 4013 5161 5406
Size: 482 657 53 392 146 677 1148 245
Cuts at: 5406 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 657 482 392 245 146
53
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5368
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5920
NotI GC'GGCC_GC
Cuts at: 3367 3367
Size: 5920
NspI r_CATG'y
Cuts at: 1132 1229 2323 3008 3278 4823 1132
Size: 97 1094 685 270 1545 2229
Fragments arranged by size:
2229 1545 1094 685 270 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5920
PciI A'CATG_T
Cuts at: 4819 4819
Size: 5920
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2890 1108
Size: 1782 4138
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 3989 4021 5502 5534 2765
Size: 32 1192 32 1481 32 3151
Fragments arranged by size:
3151 1481 1192 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 3202 3094
Size: 108 5812
PshAI GACnn'nnGTC
Cuts at: 1613 2189 1613
Size: 576 5344
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PspOMI G'GGCC_C
Cuts at: 3165 3165
Size: 5920
PstI C_TGCA'G
Cuts at: 830 2781 3141 3154 3272 830
Size: 1951 360 13 118 3478
Fragments arranged by size:
3478 1951 360 118 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
PvuII CAG'CTG
Cuts at: 2088 2088
Size: 5920
SacI G_AGCT'C
Cuts at: 2222 2537 2222
Size: 315 5605
SalI G'TCGA_C
Cuts at: 1936 3262 1936
Size: 1326 4594
SapI GCTCTTCn'nnn_
Cuts at: 2031 2279 2525 2861 2031
Size: 248 246 336 5090
Fragments arranged by size:
5090 336 248 246
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SfcI C'TryA_G
Cuts at: 826 1045 2777 3137 3150 3268 4403 5084 5275
Size: 219 1732 360 13 118 1135 681 191
Cuts at: 5275 826
Size: 1471
Fragments arranged by size:
1732 1471 1135 681 360 219 191 118
13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 2825 3247 1872
Size: 953 422 4545
Fragments arranged by size:
4545 953 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4024 4925 5187 820
Size: 197 926 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 2323 3278 1132
Size: 1191 955 3774
Fragments arranged by size:
3774 1191 955
SspI AAT'ATT
Cuts at: 5 52 3844 5
Size: 47 3792 2081
Fragments arranged by size:
3792 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5920
StyI C'CwwG_G
Cuts at: 513 1065 3359 513
Size: 552 2294 3074
Fragments arranged by size:
3074 2294 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2198 2437 3947 3964 4117 4302 4747
Size: 1255 618 239 1510 17 153 185 445
Cuts at: 4747 325
Size: 1498
Fragments arranged by size:
1510 1498 1255 618 445 239 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 2583 4166
Size: 274 80 33 51 1443 309 297 1583
Cuts at: 4166 5878 96
Size: 1712 138
Fragments arranged by size:
1712 1583 1443 309 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2412 2444 5880 5912 2412
Size: 32 3436 32 2420
Fragments arranged by size:
3436 2420 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2915 1316
Size: 1599 4321
XbaI T'CTAG_A
Cuts at: 3256 3256
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2701 2701
Size: 5920
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 2823 3245 1870
Size: 953 422 4545
Fragments arranged by size:
4545 953 422
XmnI GAAnn'nnTTC
Cuts at: 2870 4049 2870
Size: 1179 4741
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2842 3728 276
Size: 53 83 186 936 1308 886 2468
Fragments arranged by size:
2468 1308 936 886 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI