Restriction map for
pFN21AA0440
(Circular) (Six-base) MAPSORT of: pFN21AA0440.seq Check: 9610 from: 1 to: 10114
pFN21AA0440 10114 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7323 7323
Size: 10114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6843 7924 278
Size: 53 83 186 936 5307 1081 2468
Fragments arranged by size:
5307 2468 1081 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 3677 7457 1937
Size: 1413 327 3780 4594
Fragments arranged by size:
4594 3780 1413 327
Acc65I G'GTAC_C
Cuts at: 7435 7435
Size: 10114
AclI AA'CG_TT
Cuts at: 1563 8241 8614 1563
Size: 6678 373 3063
Fragments arranged by size:
6678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 3897 4620 6229 6832 7265 7280 7391
Size: 861 1404 723 1609 603 433 15 111
Cuts at: 7391 8177 9561 9829 1632
Size: 786 1384 268 1917
Fragments arranged by size:
1917 1609 1404 1384 861 786 723 603
433 268 111 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9917
AflIII A'CryG_T
Cuts at: 1203 2981 3454 9013 9990 1203
Size: 1778 473 5559 977 1327
Fragments arranged by size:
5559 1778 1327 977 473
AgeI A'CCGG_T
Cuts at: 8945 8945
Size: 10114
AhdI GACnn_n'nnGTC
Cuts at: 8843 8843
Size: 10114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 3933 3965 2438
Size: 32 1463 32 8587
Fragments arranged by size:
8587 1463 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2976 3548 3760 4105 4251 7165 7315 7857 9429
Size: 572 212 345 146 2914 150 542 1572
Cuts at: 9429 2976
Size: 3661
Fragments arranged by size:
3661 2914 1572 572 542 345 212 150
146
ApaLI G'TGCA_C
Cuts at: 4226 8171 9327 4226
Size: 3945 1156 5013
Fragments arranged by size:
5013 3945 1156
ApoI r'AATT_y
Cuts at: 1493 3181 3307 3485 3923 5174 7423 7649 7801
Size: 1688 126 178 438 1251 2249 226 152
Cuts at: 7801 9910 1493
Size: 2109 1697
Fragments arranged by size:
2249 2109 1697 1688 1251 438 226 178
152 126
AseI AT'TA_AT
Cuts at: 160 1032 4371 5783 8668 160
Size: 872 3339 1412 2885 1606
Fragments arranged by size:
3339 2885 1606 1412 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10114
AvaI C'yCGr_G
Cuts at: 1870 1943 2491 5693 5848 7439 1870
Size: 73 548 3202 155 1591 4545
Fragments arranged by size:
4545 3202 1591 548 155 73
AvrII C'CTAG_G
Cuts at: 6413 6413
Size: 10114
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6259 6292 6259
Size: 33 10081
BamHI G'GATC_C
Cuts at: 5794 7444 7811 5794
Size: 1650 367 8097
Fragments arranged by size:
8097 1650 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 5361 5966 6082 7435
Size: 325 217 627 291 3283 605 116 1353
Cuts at: 7435 8890 618
Size: 1455 1842
Fragments arranged by size:
3283 1842 1455 1353 627 605 325 291
217 116
BanII G_rGCy'C
Cuts at: 1391 3636 3747 4563 4941 5771 6287 7433 1391
Size: 2245 111 816 378 830 516 1146 4072
Fragments arranged by size:
4072 2245 1146 830 816 516 378 111
BbeI G_GCGC'C
Cuts at: 5365 5365
Size: 10114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2967 4608 4684 5457 6165 7287 7384
Size: 971 1068 1641 76 773 708 1122 97
Cuts at: 7384 928
Size: 3658
Fragments arranged by size:
3658 1641 1122 1068 971 773 708 97
76
BbvCI CC'TCA_GC
Cuts at: 3197 4967 3197
Size: 1770 8344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4961 4995 8305 8339 1903
Size: 34 3024 34 3310 34 3678
Fragments arranged by size:
3678 3310 3024 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8006 9216 8006
Size: 1210 8904
BclI T'GATC_A
Cuts at: 1548 7144 1548
Size: 5596 4518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4958 5614 6210
Size: 107 122 71 631 605 3286 656 596
Cuts at: 6210 8725 136
Size: 2515 1525
Fragments arranged by size:
3286 2515 1525 656 631 605 596 122
107 71
BglII A'GATC_T
Cuts at: 2938 3460 4447 4992 6622 10109 2938
Size: 522 987 545 1630 3487 2943
Fragments arranged by size:
3487 2943 1630 987 545 522
BlpI GC'TnA_GC
Cuts at: 4562 7531 4562
Size: 2969 7145
Bme1580I G_kGCm'C
Cuts at: 1790 3402 4230 5498 5896 6325 8175 9331 1790
Size: 1612 828 1268 398 429 1850 1156 2573
Fragments arranged by size:
2573 1850 1612 1268 1156 828 429 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5431 5569 5766
Size: 416 53 83 399 24 4014 138 197
Cuts at: 5766 6299 7845 8803 442
Size: 533 1546 958 1753
Fragments arranged by size:
4014 1753 1546 958 533 416 399 197
138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2834 3971 4577 2517
Size: 317 1137 606 8054
Fragments arranged by size:
8054 1137 606 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2440 2679 2895 3108 3680 3746 4179 5301
Size: 1201 239 216 213 572 66 433 1122
Cuts at: 5301 5335 5842 5860 6085 6787 7172 8774 1239
Size: 34 507 18 225 702 385 1602 2579
Fragments arranged by size:
2579 1602 1201 1122 702 572 507 433
385 239 225 216 213 66 34 18
Bpu10I CC'TnA_GC
Cuts at: 2140 3197 3741 4967 6058 6179 6591 2140
Size: 1057 544 1226 1091 121 412 5663
Fragments arranged by size:
5663 1226 1091 1057 544 412 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 2834 5226 7144 8239 9104 9402 2296
Size: 538 2392 1918 1095 865 298 3008
Fragments arranged by size:
3008 2392 1918 1095 865 538 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3124 5416 6567 6965 8777 882
Size: 617 1625 2292 1151 398 1812 2219
Fragments arranged by size:
2292 2219 1812 1625 1151 617 398
BsaAI yAC'GTr
Cuts at: 493 1204 3457 3733 6858 7905 493
Size: 711 2253 276 3125 1047 2702
Fragments arranged by size:
3125 2702 2253 1047 711 276
BsaBI GATnn'nnATC
Cuts at: 3531 10108 3531
Size: 6577 3537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3666 5362 6809 6840
Size: 53 83 186 936 2133 1696 1447 31
Cuts at: 6840 7921 8303 8938 275
Size: 1081 382 635 1451
Fragments arranged by size:
2133 1696 1451 1447 1081 936 635 382
186 83 53 31
BsaWI w'CCGG_w
Cuts at: 2121 6159 8547 8945 9219 9366 9777 2121
Size: 4038 2388 398 274 147 411 2458
Fragments arranged by size:
4038 2458 2388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 2471 2501 3372
Size: 30 165 30 1035 30 3 30 871
Cuts at: 3372 3402 3933 3963 4086 4116 4758 4788 6677
Size: 30 531 30 123 30 642 30 1889
Cuts at: 6677 6707 1178
Size: 30 4585
Fragments arranged by size:
4585 1889 1035 871 642 531 165 123
30 30 30 30 30 30 30 30
30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2872 3085 4779 4817 5122 5304 6376 6379
Size: 1686 213 1694 38 305 182 1072 3
Cuts at: 6379 6388 6391 6394 6685 6690 6904 7189 7358
Size: 9 3 3 291 5 214 285 169
Cuts at: 7358 1186
Size: 3942
Fragments arranged by size:
3942 1694 1686 1072 305 291 285 214
213 182 169 38 9 5 3 3
3
BseYI C'CCAG_C
Cuts at: 2483 3910 5548 5731 6008 6031 6716 9317 9813
Size: 1427 1638 183 277 23 685 2601 496
Cuts at: 9813 2483
Size: 2784
Fragments arranged by size:
2784 2601 1638 1427 685 496 277 183
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 5080 5279 7328 1163
Size: 573 858 2486 199 2049 3949
Fragments arranged by size:
3949 2486 2049 858 573 199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7564 8325 9353 1540
Size: 275 186 5563 761 1028 2301
Fragments arranged by size:
5563 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3636 3732 3747 3875 3982 4165 4230 4241 4563
Size: 96 15 128 107 183 65 11 322
Cuts at: 4563 4665 6287 6792 8175 8260 9331 3636
Size: 102 1622 505 1383 85 1071 4419
Fragments arranged by size:
4419 1622 1383 1071 505 322 183 128
107 102 96 85 65 15 11
BsmI GAATG_Cn'
Cuts at: 3555 5290 5830 7625 7718 3555
Size: 1735 540 1795 93 5951
Fragments arranged by size:
5951 1795 1735 540 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3402 3636 3732 3747 3875 3982 4165
Size: 399 1612 234 96 15 128 107 183
Cuts at: 4165 4230 4241 4563 4665 4941 5498 5771 5896
Size: 65 11 322 102 276 557 273 125
Cuts at: 5896 6287 6325 6792 7433 8175 8260 9331 1391
Size: 391 38 467 641 742 85 1071 2174
Fragments arranged by size:
2174 1612 1071 742 641 557 467 399
391 322 276 273 234 183 128 125
107 102 96 85 65 38 15 11
BspEI T'CCGG_A
Cuts at: 9777 9777
Size: 10114
BspHI T'CATG_A
Cuts at: 3636 3801 8003 9733 3636
Size: 165 4202 1730 4017
Fragments arranged by size:
4202 4017 1730 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7323 7469 844
Size: 6479 146 3489
Fragments arranged by size:
6479 3489 146
BsrBI CCG'CTC
Cuts at: 1394 1823 8001 1394
Size: 429 6178 3507
Fragments arranged by size:
6178 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2911 3232 3364 5634 5887 6541 8609
Size: 1183 1663 321 132 2270 253 654 2068
Cuts at: 8609 8783 65
Size: 174 1396
Fragments arranged by size:
2270 2068 1663 1396 1183 654 321 253
174 132
BsrFI r'CCGG_y
Cuts at: 1700 1810 6209 8758 8945 1700
Size: 110 4399 2549 187 2869
Fragments arranged by size:
4399 2869 2549 187 110
BsrGI T'GTAC_A
Cuts at: 96 3888 4023 96
Size: 3792 135 6187
Fragments arranged by size:
6187 3792 135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10114
BssSI C'ACGA_G
Cuts at: 6508 8174 9186 6508
Size: 1666 1012 7436
Fragments arranged by size:
7436 1666 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 4382 6988 7315 794
Size: 1449 2139 2606 327 3593
Fragments arranged by size:
3593 2606 2139 1449 327
BstBI TT'CG_AA
Cuts at: 3559 8918 3559
Size: 5359 4755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10114
BstXI CCAn_nnnn'nTGG
Cuts at: 4157 6307 4157
Size: 2150 7964
BstYI r'GATC_y
Cuts at: 1972 2938 3460 3593 4447 4641 4992 5432 5543
Size: 966 522 133 854 194 351 440 111
Cuts at: 5543 5794 6622 7444 7811 8196 8213 9665 9751
Size: 251 828 822 367 385 17 1452 86
Cuts at: 9751 9763 10109 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 966 854 828 822 522 440
385 367 351 346 251 194 133 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2819 4106 5670 6907 7331 2819
Size: 1287 1564 1237 424 5602
Fragments arranged by size:
5602 1564 1287 1237 424
BtgI C'CryG_G
Cuts at: 513 1065 3105 4413 4617 5102 5418 6013 6117
Size: 552 2040 1308 204 485 316 595 104
Cuts at: 6117 6148 6492 513
Size: 31 344 4135
Fragments arranged by size:
4135 2040 1308 595 552 485 344 316
204 104 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6543 7100 487
Size: 683 56 5317 557 3501
Fragments arranged by size:
5317 3501 683 557 56
BtsI GCAGTG_nn'
Cuts at: 2606 7020 7630 8424 8444 2606
Size: 4414 610 794 20 4276
Fragments arranged by size:
4414 4276 794 610 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4668 4703 529
Size: 35 4104 35 5940
Fragments arranged by size:
5940 4104 35 35
DraI TTT'AAA
Cuts at: 7418 7418
Size: 10114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 9121 809
Size: 507 1121 6684 1802
Fragments arranged by size:
6684 1802 1121 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 7561 8450 10079
Size: 54 1425 50 275 18 5731 889 1629
Cuts at: 10079 8
Size: 43
Fragments arranged by size:
5731 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7561 1537
Size: 275 5749 4090
Fragments arranged by size:
5749 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3876 6336 6397 6665 6851 8044 1353
Size: 1155 1368 2460 61 268 186 1193 3423
Fragments arranged by size:
3423 2460 1368 1193 1155 268 186 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4943 7316 8701 9075 9221 387
Size: 1064 138 3354 2373 1385 374 146 1280
Fragments arranged by size:
3354 2373 1385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3634 3745 4561 6285 3634
Size: 111 816 1724 7463
Fragments arranged by size:
7463 1724 816 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2440 2493 2679 2895 3108 3680 3746
Size: 393 808 53 186 216 213 572 66
Cuts at: 3746 3897 4179 4620 5301 5335 5842 5860 6085
Size: 151 282 441 681 34 507 18 225
Cuts at: 6085 6229 6787 6832 7172 7265 7280 7391 8177
Size: 144 558 45 340 93 15 111 786
Cuts at: 8177 8774 9561 9829 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 808 787 786 681 597 572 558
507 441 393 340 282 268 225 216
213 186 151 144 111 93 66 53
45 34 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 6057 7319 6057
Size: 1262 8852
EcoO109I rG'GnC_Cy
Cuts at: 2055 2168 2973 3175 5228 5666 2055
Size: 113 805 202 2053 438 6503
Fragments arranged by size:
6503 2053 805 438 202 113
EcoRI G'AATT_C
Cuts at: 3181 3923 5174 7423 7801 3181
Size: 742 1251 2249 378 5494
Fragments arranged by size:
5494 2249 1251 742 378
EcoRV GAT'ATC
Cuts at: 2536 4132 2536
Size: 1596 8518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3427 3944 5203 6362 7310 3427
Size: 517 1259 1159 948 6231
Fragments arranged by size:
6231 1259 1159 948 517
FspI TGC'GCA
Cuts at: 2903 8620 2903
Size: 5717 4397
HaeII r_GCGC'y
Cuts at: 1678 2119 5365 6441 9261 1678
Size: 441 3246 1076 2820 2531
Fragments arranged by size:
3246 2820 2531 1076 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2315 2347 2687
Size: 32 135 32 470 32 241 32 340
Cuts at: 2687 2719 2939 2971 3639 3671 5299 5331 5981
Size: 32 220 32 668 32 1628 32 650
Cuts at: 5981 6013 6502 6534 8755 8787 8829 8861 1373
Size: 32 489 32 2221 32 42 32 2626
Fragments arranged by size:
2626 2221 1628 668 650 489 470 340
241 220 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 4909 7458 7704 669
Size: 988 281 1740 1231 2549 246 3079
Fragments arranged by size:
3079 2549 1740 1231 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2665 748
Size: 1917 8197
HpaI GTT'AAC
Cuts at: 7704 7704
Size: 10114
KasI G'GCGC_C
Cuts at: 5361 5361
Size: 10114
KpnI G_GTAC'C
Cuts at: 7439 7439
Size: 10114
MfeI C'AATT_G
Cuts at: 2637 4140 4696 7713 2637
Size: 1503 556 3017 5038
Fragments arranged by size:
5038 3017 1503 556
MluI A'CGCG_T
Cuts at: 9990 9990
Size: 10114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 2741 2747 5423 6738 7068 7161 9228
Size: 1116 320 6 2676 1315 330 93 2067
Cuts at: 9228 9412 1305
Size: 184 2007
Fragments arranged by size:
2676 2067 2007 1315 1116 330 320 184
93 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 8292
Fragments arranged by size:
8292 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2097 3006 5135 5402 5901 6088 7406
Size: 648 931 909 2129 267 499 187 1318
Cuts at: 7406 8072 8431 8590 518
Size: 666 359 159 2042
Fragments arranged by size:
2129 2042 1318 931 909 666 648 499
359 267 187 159
MspA1I CmG'CkG
Cuts at: 1606 3101 3905 5104 5595 6356 7150 7231 7530
Size: 1495 804 1199 491 761 794 81 299
Cuts at: 7530 8207 9355 9600 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1495 1199 1148 804 794 761 677
491 299 245 81
NaeI GCC'GGC
Cuts at: 6211 6211
Size: 10114
NarI GG'CG_CC
Cuts at: 5362 5362
Size: 10114
NcoI C'CATG_G
Cuts at: 513 1065 4413 4617 5418 6117 513
Size: 552 3348 204 801 699 4510
Fragments arranged by size:
4510 3348 801 699 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10114
NgoMIV G'CCGG_C
Cuts at: 6209 6209
Size: 10114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10114
NotI GC'GGCC_GC
Cuts at: 7561 7561
Size: 10114
NspI r_CATG'y
Cuts at: 1132 1229 2113 2985 5134 5724 7472 9017 1132
Size: 97 884 872 2149 590 1748 1545 2229
Fragments arranged by size:
2229 2149 1748 1545 884 872 590 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10114
PciI A'CATG_T
Cuts at: 2981 9013 2981
Size: 6032 4082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4584 6154 1108
Size: 3476 1570 5068
Fragments arranged by size:
5068 3476 1570
PfoI T'CCnGG_A
Cuts at: 3657 4549 6966 3657
Size: 892 2417 6805
Fragments arranged by size:
6805 2417 892
PmeI GTTT'AAAC
Cuts at: 7418 7418
Size: 10114
PmlI CAC'GTG
Cuts at: 3733 3733
Size: 10114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 3933 3965 4003 4035 8183 8215 9696
Size: 32 1463 32 38 32 4148 32 1481
Cuts at: 9696 9728 2438
Size: 32 2824
Fragments arranged by size:
4148 2824 1481 1463 38 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2168 3175 5666 2168
Size: 1007 2491 6616
Fragments arranged by size:
6616 2491 1007
PshAI GACnn'nnGTC
Cuts at: 1613 3130 1613
Size: 1517 8597
PsiI TTA'TAA
Cuts at: 7684 7684
Size: 10114
PspXI vC'TCGA_Gb
Cuts at: 5693 5848 5693
Size: 155 9959
PstI C_TGCA'G
Cuts at: 830 7030 7466 830
Size: 6200 436 3478
Fragments arranged by size:
6200 3478 436
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10114
PvuII CAG'CTG
Cuts at: 3101 3905 6356 7150 7231 3101
Size: 804 2451 794 81 5984
Fragments arranged by size:
5984 2451 804 794 81
SacI G_AGCT'C
Cuts at: 3636 3747 4563 6287 3636
Size: 111 816 1724 7463
Fragments arranged by size:
7463 1724 816 111
SacII CC_GC'GG
Cuts at: 5105 5105
Size: 10114
SalI G'TCGA_C
Cuts at: 1936 3676 7456 1936
Size: 1740 3780 4594
Fragments arranged by size:
4594 3780 1740
SapI GCTCTTCn'nnn_
Cuts at: 2508 6336 2508
Size: 3828 6286
SbfI CC_TGCA'GG
Cuts at: 7466 7466
Size: 10114
ScaI AGT'ACT
Cuts at: 3390 8362 10074 3390
Size: 4972 1712 3430
Fragments arranged by size:
4972 3430 1712
SexAI A'CCwGG_T
Cuts at: 4271 7047 4271
Size: 2776 7338
SfcI C'TryA_G
Cuts at: 826 1045 2163 4705 7026 7337 7462 8597 9278
Size: 219 1118 2542 2321 311 125 1135 681
Cuts at: 9278 9469 826
Size: 191 1471
Fragments arranged by size:
2542 2321 1471 1135 1118 681 311 219
191 125
SfoI GGC'GCC
Cuts at: 5363 5363
Size: 10114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10114
SmaI CCC'GGG
Cuts at: 1872 7441 1872
Size: 5569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2311 2849 5241 5693 5848 7123
Size: 197 926 368 538 2392 452 155 1275
Cuts at: 7123 8218 9119 9381 820
Size: 1095 901 262 1553
Fragments arranged by size:
2392 1553 1275 1095 926 901 538 452
368 262 197 155
SnaBI TAC'GTA
Cuts at: 493 7905 493
Size: 7412 2702
SpeI A'CTAG_T
Cuts at: 152 3626 152
Size: 3474 6640
SphI G_CATG'C
Cuts at: 1132 5134 7472 1132
Size: 4002 2338 3774
Fragments arranged by size:
4002 3774 2338
SspI AAT'ATT
Cuts at: 5 52 8038 5
Size: 47 7986 2081
Fragments arranged by size:
7986 2081 47
StuI AGG'CCT
Cuts at: 1517 2038 3705 6895 1517
Size: 521 1667 3190 4736
Fragments arranged by size:
4736 3190 1667 521
StyI C'CwwG_G
Cuts at: 513 1065 3114 3402 3783 4413 4617 5418 6117
Size: 552 2049 288 381 630 204 801 699
Cuts at: 6117 6413 7553 513
Size: 296 1140 3074
Fragments arranged by size:
3074 2049 1140 801 699 630 552 381
296 288 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3643 3796 4099 5588 8141 8158
Size: 1255 1875 188 153 303 1489 2553 17
Cuts at: 8158 8311 8496 8941 325
Size: 153 185 445 1498
Fragments arranged by size:
2553 1875 1498 1489 1255 445 303 188
185 153 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3388 3852 3888
Size: 274 80 33 51 1443 1411 464 36
Cuts at: 3888 4023 5145 8360 10072 96
Size: 135 1122 3215 1712 138
Fragments arranged by size:
3215 1712 1443 1411 1122 464 274 138
135 80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5229 5261 6677 6709 10074 10106 5229
Size: 32 1416 32 3365 32 5237
Fragments arranged by size:
5237 3365 1416 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2966 4247 1316
Size: 1650 1281 7183
Fragments arranged by size:
7183 1650 1281
XbaI T'CTAG_A
Cuts at: 7450 7450
Size: 10114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3409 5582 6490 3409
Size: 2173 908 7033
Fragments arranged by size:
7033 2173 908
XhoI C'TCGA_G
Cuts at: 1943 5693 5848 1943
Size: 3750 155 6209
Fragments arranged by size:
6209 3750 155
XmaI C'CCGG_G
Cuts at: 1870 7439 1870
Size: 5569 4545
XmnI GAAnn'nnTTC
Cuts at: 3927 4015 6345 7376 8243 3927
Size: 88 2330 1031 867 5798
Fragments arranged by size:
5798 2330 1031 867 88
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6841 7922 276
Size: 53 83 186 936 5307 1081 2468
Fragments arranged by size:
5307 2468 1081 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BfrBI BsiWI BsmBI BstZ17I DraIII
FseI FspAI NruI NsiI PacI PspOMI PsrI RsrII
SanDI SfiI SrfI SwaI