Restriction map for
pFN21AA0439
(Circular) (Six-base) MAPSORT of: pFN21AA0439.seq Check: 6401 from: 1 to: 7207
pFN21AA0439 7207 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5017 278
Size: 53 83 186 936 3481 2468
Fragments arranged by size:
3481 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4550 1937
Size: 2613 4594
Acc65I G'GTAC_C
Cuts at: 4528 4528
Size: 7207
AclI AA'CG_TT
Cuts at: 1563 3272 5334 5707 1563
Size: 1709 2062 373 3063
Fragments arranged by size:
3063 2062 1709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 2730 3376 3644 5270 6654 6922 1632
Size: 938 160 646 268 1626 1384 268 1917
Fragments arranged by size:
1917 1626 1384 938 646 268 268 160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7010
AflIII A'CryG_T
Cuts at: 1203 2325 6106 7083 1203
Size: 1122 3781 977 1327
Fragments arranged by size:
3781 1327 1122 977
AgeI A'CCGG_T
Cuts at: 6038 6038
Size: 7207
AhdI GACnn_n'nnGTC
Cuts at: 3506 5936 3506
Size: 2430 4777
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 7175
AlwNI CAG_nnn'CTG
Cuts at: 2560 4950 6522 2560
Size: 2390 1572 3245
Fragments arranged by size:
3245 2390 1572
ApaLI G'TGCA_C
Cuts at: 5264 6420 5264
Size: 1156 6051
ApoI r'AATT_y
Cuts at: 1493 3173 3252 3368 4104 4516 4742 4894 7003
Size: 1680 79 116 736 412 226 152 2109
Cuts at: 7003 1493
Size: 1697
Fragments arranged by size:
2109 1697 1680 736 412 226 152 116
79
AseI AT'TA_AT
Cuts at: 160 1032 5761 160
Size: 872 4729 1606
Fragments arranged by size:
4729 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2231 2423 2450 4532 1870
Size: 73 118 170 192 27 2082 4545
Fragments arranged by size:
4545 2082 192 170 118 73 27
BamHI G'GATC_C
Cuts at: 3867 4537 4904 3867
Size: 670 367 6170
Fragments arranged by size:
6170 670 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2960 4528 5983 618
Size: 325 217 627 1173 1568 1455 1842
Fragments arranged by size:
1842 1568 1455 1173 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 2430 4399 4526 1391
Size: 1033 6 1969 127 4072
Fragments arranged by size:
4072 1969 1033 127 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 3318 928
Size: 971 519 900 4817
Fragments arranged by size:
4817 971 900 519
BbvCI CC'TCA_GC
Cuts at: 2921 4347 2921
Size: 1426 5781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2663 2697 5398 5432 1903
Size: 34 726 34 2701 34 3678
Fragments arranged by size:
3678 2701 726 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5099 6309 4277
Size: 822 1210 5175
Fragments arranged by size:
5175 1210 822
BclI T'GATC_A
Cuts at: 1548 3130 3283 1548
Size: 1582 153 5472
Fragments arranged by size:
5472 1582 153
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5818 136
Size: 107 122 71 631 605 4146 1525
Fragments arranged by size:
4146 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7202 7202
Size: 7207
BlpI GC'TnA_GC
Cuts at: 4624 4624
Size: 7207
Bme1580I G_kGCm'C
Cuts at: 1790 5268 6424 1790
Size: 3478 1156 2573
Fragments arranged by size:
3478 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2326 1318
Size: 1008 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4938 5896 442
Size: 416 53 83 399 24 3521 958 1753
Fragments arranged by size:
3521 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2516
Size: 7207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 2580 2975 3346 3893 4145 4189 5867
Size: 1272 69 395 371 547 252 44 1678
Cuts at: 5867 1239
Size: 2579
Fragments arranged by size:
2579 1678 1272 547 395 371 252 69
44
Bpu10I CC'TnA_GC
Cuts at: 2772 2921 4347 2772
Size: 149 1426 5632
Fragments arranged by size:
5632 1426 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 4470 5332 6197 6495 2632
Size: 1838 862 865 298 3344
Fragments arranged by size:
3344 1838 865 862 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 5870 882
Size: 617 957 3414 2219
Fragments arranged by size:
3414 2219 957 617
BsaAI yAC'GTr
Cuts at: 493 1204 4998 493
Size: 711 3794 2702
Fragments arranged by size:
3794 2702 711
BsaBI GATnn'nnATC
Cuts at: 7201 7201
Size: 7207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4254 5014 5396 6031
Size: 53 83 186 936 2721 760 382 635
Cuts at: 6031 275
Size: 1451
Fragments arranged by size:
2721 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2289 2858 4269 5640 6038 6312 6459 6870 2289
Size: 569 1411 1371 398 274 147 411 2626
Fragments arranged by size:
2626 1411 1371 569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6982
Fragments arranged by size:
6982 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2205 2208 2211 2635 1186
Size: 841 178 3 3 424 5758
Fragments arranged by size:
5758 841 424 178 3 3
BseYI C'CCAG_C
Cuts at: 2751 3087 3240 3331 6410 6906 2751
Size: 336 153 91 3079 496 3052
Fragments arranged by size:
3079 3052 496 336 153 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6634
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 4657 5418 6446 1540
Size: 275 186 1114 1542 761 1028 2301
Fragments arranged by size:
2301 1542 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4399 5268 5353 6424 4399
Size: 869 85 1071 5182
Fragments arranged by size:
5182 1071 869 85
BsiWI C'GTAC_G
Cuts at: 2977 2977
Size: 7207
BsmI GAATG_Cn'
Cuts at: 4177 4718 4811 4177
Size: 541 93 6573
Fragments arranged by size:
6573 541 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2430 4399 4526 5268 5353 6424
Size: 399 634 6 1969 127 742 85 1071
Cuts at: 6424 1391
Size: 2174
Fragments arranged by size:
2174 1969 1071 742 634 399 127 85
6
BspEI T'CCGG_A
Cuts at: 2858 6870 2858
Size: 4012 3195
BspHI T'CATG_A
Cuts at: 5096 6826 5096
Size: 1730 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3421 4562 844
Size: 2577 1141 3489
Fragments arranged by size:
3489 2577 1141
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 5094 1394
Size: 429 568 2703 3507
Fragments arranged by size:
3507 2703 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 5702 5876 65
Size: 1183 3125 1329 174 1396
Fragments arranged by size:
3125 1396 1329 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2524 2907 3114 5851 6038 1700
Size: 110 569 145 383 207 2737 187 2869
Fragments arranged by size:
2869 2737 569 383 207 187 145 110
BsrGI T'GTAC_A
Cuts at: 96 2722 96
Size: 2626 4581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7207
BssSI C'ACGA_G
Cuts at: 5267 6279 5267
Size: 1012 6195
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7207
BstBI TT'CG_AA
Cuts at: 6011 6011
Size: 7207
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7207
BstXI CCAn_nnnn'nTGG
Cuts at: 3087 3087
Size: 7207
BstYI r'GATC_y
Cuts at: 1972 2537 2901 3160 3867 4537 4904 5289 5306
Size: 565 364 259 707 670 367 385 17
Cuts at: 5306 6758 6844 6856 7202 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 707 670 565 385 367 364
346 259 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2553 2553
Size: 7207
BtgI C'CryG_G
Cuts at: 513 1065 2004 3642 4473 513
Size: 552 939 1638 831 3247
Fragments arranged by size:
3247 1638 939 831 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4281 487
Size: 683 56 3055 3413
Fragments arranged by size:
3413 3055 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 4723 5517 5537 2671
Size: 2052 794 20 4341
Fragments arranged by size:
4341 2052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7172
DraI TTT'AAA
Cuts at: 3207 4511 3207
Size: 1304 5903
DraIII CAC_nnn'GTG
Cuts at: 2301 2301
Size: 7207
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2049 6214 809
Size: 507 733 4165 1802
Fragments arranged by size:
4165 1802 733 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3112 3265 3570
Size: 54 1425 50 275 18 1282 153 305
Cuts at: 3570 3738 4470 4654 5543 7172 8
Size: 168 732 184 889 1629 43
Fragments arranged by size:
1629 1425 1282 889 732 305 275 184
168 153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 4654 1537
Size: 275 1300 1542 4090
Fragments arranged by size:
4090 1542 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3462 3718 5137 1353
Size: 2109 256 1419 3423
Fragments arranged by size:
3423 2109 1419 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2894 5794 6168 6314 387
Size: 1064 138 1305 2900 374 146 1280
Fragments arranged by size:
2900 1305 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4397 4397
Size: 7207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 2570 2580 2730 2975 3346 3376
Size: 393 879 59 10 150 245 371 30
Cuts at: 3376 3644 3893 4145 4189 5270 5867 6654 6922
Size: 268 249 252 44 1081 597 787 268
Cuts at: 6922 1239
Size: 1524
Fragments arranged by size:
1524 1081 879 787 597 393 371 268
268 252 249 245 150 59 44 30
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2647 3675 2647
Size: 1028 6179
EcoRI G'AATT_C
Cuts at: 3252 4516 4894 3252
Size: 1264 378 5565
Fragments arranged by size:
5565 1264 378
EcoRV GAT'ATC
Cuts at: 3421 3421
Size: 7207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2225 3017 2225
Size: 792 6415
FseI GG_CCGG'CC
Cuts at: 3118 3118
Size: 7207
FspI TGC'GCA
Cuts at: 5713 5713
Size: 7207
HaeII r_GCGC'y
Cuts at: 1678 2386 3107 6354 1678
Size: 708 721 3247 2531
Fragments arranged by size:
3247 2531 721 708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2536 2568 2840
Size: 32 135 32 743 32 189 32 272
Cuts at: 2840 2872 3492 3524 3556 3686 3718 3815 3847
Size: 32 620 32 32 130 32 97 32
Cuts at: 3847 5848 5880 5922 5954 1373
Size: 2001 32 42 32 2626
Fragments arranged by size:
2626 2001 743 620 272 189 135 130
97 42 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 2278 4551 4797 669
Size: 988 281 235 105 2273 246 3079
Fragments arranged by size:
3079 2273 988 281 246 235 105
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7207
HpaI GTT'AAC
Cuts at: 4797 4797
Size: 7207
KpnI G_GTAC'C
Cuts at: 4532 4532
Size: 7207
MfeI C'AATT_G
Cuts at: 4806 4806
Size: 7207
MluI A'CGCG_T
Cuts at: 7083 7083
Size: 7207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 3030 6321 6505 1305
Size: 1014 711 3291 184 2007
Fragments arranged by size:
3291 2007 1014 711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3572 10
Size: 54 1425 343 1740 3645
Fragments arranged by size:
3645 1740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3261 4472 5165 5524 5683 518
Size: 648 2095 1211 693 359 159 2042
Fragments arranged by size:
2095 2042 1211 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2953 4351 4623 5300 6448 6693 1606
Size: 1347 1398 272 677 1148 245 2120
Fragments arranged by size:
2120 1398 1347 1148 677 272 245
NaeI GCC'GGC
Cuts at: 2909 3116 2909
Size: 207 7000
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5716
Fragments arranged by size:
5716 939 552
NdeI CA'TA_TG
Cuts at: 387 3186 4432 387
Size: 2799 1246 3162
Fragments arranged by size:
3162 2799 1246
NgoMIV G'CCGG_C
Cuts at: 2907 3114 2907
Size: 207 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7207
NotI GC'GGCC_GC
Cuts at: 4654 4654
Size: 7207
NruI TCG'CGA
Cuts at: 4300 4300
Size: 7207
NspI r_CATG'y
Cuts at: 1132 1229 4407 4565 6110 1132
Size: 97 3178 158 1545 2229
Fragments arranged by size:
3178 2229 1545 158 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7207
PciI A'CATG_T
Cuts at: 6106 6106
Size: 7207
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2108 2301 2817 3579 1108
Size: 1000 193 516 762 4736
Fragments arranged by size:
4736 1000 762 516 193
PfoI T'CCnGG_A
Cuts at: 3870 3870
Size: 7207
PmeI GTTT'AAAC
Cuts at: 4511 4511
Size: 7207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5276 5308 6789 6821 5276
Size: 32 1481 32 5662
Fragments arranged by size:
5662 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7207
PsiI TTA'TAA
Cuts at: 4777 4777
Size: 7207
PstI C_TGCA'G
Cuts at: 830 3327 4281 4559 830
Size: 2497 954 278 3478
Fragments arranged by size:
3478 2497 954 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7207
PvuII CAG'CTG
Cuts at: 4351 4351
Size: 7207
SacI G_AGCT'C
Cuts at: 4399 4399
Size: 7207
SalI G'TCGA_C
Cuts at: 1936 4549 1936
Size: 2613 4594
SbfI CC_TGCA'GG
Cuts at: 4559 4559
Size: 7207
ScaI AGT'ACT
Cuts at: 3635 5455 7167 3635
Size: 1820 1712 3675
Fragments arranged by size:
3675 1820 1712
SfcI C'TryA_G
Cuts at: 826 1045 3323 4277 4555 5690 6371 6562 826
Size: 219 2278 954 278 1135 681 191 1471
Fragments arranged by size:
2278 1471 1135 954 681 278 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
SgrAI Cr'CCGG_yG
Cuts at: 1700 2379 1700
Size: 679 6528
SmaI CCC'GGG
Cuts at: 1872 2233 4534 1872
Size: 361 2301 4545
Fragments arranged by size:
4545 2301 361
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2061 2647 4485 5311 6212 6474
Size: 197 926 118 586 1838 826 901 262
Cuts at: 6474 820
Size: 1553
Fragments arranged by size:
1838 1553 926 901 826 586 262 197
118
SnaBI TAC'GTA
Cuts at: 493 4998 493
Size: 4505 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7207
SphI G_CATG'C
Cuts at: 1132 4565 1132
Size: 3433 3774
SspI AAT'ATT
Cuts at: 5 52 3848 5131 5
Size: 47 3796 1283 2081
Fragments arranged by size:
3796 2081 1283 47
StuI AGG'CCT
Cuts at: 1517 3681 1517
Size: 2164 5043
StyI C'CwwG_G
Cuts at: 513 1065 2004 2456 2964 3220 4646 513
Size: 552 939 452 508 256 1426 3074
Fragments arranged by size:
3074 1426 939 552 508 452 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 5234 5251 5404 5589 6034 325
Size: 1255 912 2742 17 153 185 445 1498
Fragments arranged by size:
2742 1498 1255 912 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2722 3181 3633
Size: 274 80 33 51 1443 745 459 452
Cuts at: 3633 5453 7165 96
Size: 1820 1712 138
Fragments arranged by size:
1820 1712 1443 745 459 452 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7167 7199 7167
Size: 32 7175
Tth111I GACn'n_nGTC
Cuts at: 1316 4010 1316
Size: 2694 4513
XbaI T'CTAG_A
Cuts at: 4543 4543
Size: 7207
XhoI C'TCGA_G
Cuts at: 1943 2061 1943
Size: 118 7089
XmaI C'CCGG_G
Cuts at: 1870 2231 4532 1870
Size: 361 2301 4545
Fragments arranged by size:
4545 2301 361
XmnI GAAnn'nnTTC
Cuts at: 3252 4058 4083 5336 3252
Size: 806 25 1253 5123
Fragments arranged by size:
5123 1253 806 25
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5015 276
Size: 53 83 186 936 3481 2468
Fragments arranged by size:
3481 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsmBI BstZ17I EcoO109I FspAI KasI NarI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI