Restriction map for
pFN21AA0437
(Circular) (Six-base) MAPSORT of: pFN21AA0437.seq Check: 4182 from: 1 to: 9493
pFN21AA0437 9493 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5361 5361
Size: 9493
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7303 278
Size: 53 83 186 936 5767 2468
Fragments arranged by size:
5767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5699 6836 1937
Size: 3762 1137 4594
Fragments arranged by size:
4594 3762 1137
Acc65I G'GTAC_C
Cuts at: 6814 6814
Size: 9493
AclI AA'CG_TT
Cuts at: 1563 7620 7993 1563
Size: 6057 373 3063
Fragments arranged by size:
6057 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2921 4094 5019 6209 6326 7556 8940
Size: 701 588 1173 925 1190 117 1230 1384
Cuts at: 8940 9208 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1230 1190 1173 925 701 588
268 117
AflII C'TTAA_G
Cuts at: 820 1017 2023 820
Size: 197 1006 8290
Fragments arranged by size:
8290 1006 197
AflIII A'CryG_T
Cuts at: 1203 2820 5841 8392 9369 1203
Size: 1617 3021 2551 977 1327
Fragments arranged by size:
3021 2551 1617 1327 977
AgeI A'CCGG_T
Cuts at: 2609 8324 2609
Size: 5715 3778
AhdI GACnn_n'nnGTC
Cuts at: 5044 5695 8222 5044
Size: 651 2527 6315
Fragments arranged by size:
6315 2527 651
AleI CACnn'nnGTG
Cuts at: 1166 4528 1166
Size: 3362 6131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6461 1184
Size: 5277 4216
AlwNI CAG_nnn'CTG
Cuts at: 2135 2726 4864 7236 8808 2135
Size: 591 2138 2372 1572 2820
Fragments arranged by size:
2820 2372 2138 1572 591
ApaLI G'TGCA_C
Cuts at: 3529 7550 8706 3529
Size: 4021 1156 4316
Fragments arranged by size:
4316 4021 1156
ApoI r'AATT_y
Cuts at: 1493 2273 2359 3578 3944 6802 7028 7180 9289
Size: 780 86 1219 366 2858 226 152 2109
Cuts at: 9289 1493
Size: 1697
Fragments arranged by size:
2858 2109 1697 1219 780 366 226 152
86
AseI AT'TA_AT
Cuts at: 160 1032 4143 4968 8047 160
Size: 872 3111 825 3079 1606
Fragments arranged by size:
3111 3079 1606 872 825
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9493
AvaI C'yCGr_G
Cuts at: 1870 1943 6552 6648 6765 6818 1870
Size: 73 4609 96 117 53 4545
Fragments arranged by size:
4609 4545 117 96 73 53
AvrII C'CTAG_G
Cuts at: 5494 5494
Size: 9493
BamHI G'GATC_C
Cuts at: 2145 3238 3385 5490 6823 7190 2145
Size: 1093 147 2105 1333 367 4448
Fragments arranged by size:
4448 2105 1333 1093 367 147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4990 5098 6559 6814
Size: 325 217 627 1439 1764 108 1461 255
Cuts at: 6814 8269 618
Size: 1455 1842
Fragments arranged by size:
1842 1764 1461 1455 1439 627 325 255
217 108
BanII G_rGCy'C
Cuts at: 1391 2772 2979 3678 4379 4661 5595 6482 6498
Size: 1381 207 699 701 282 934 887 16
Cuts at: 6498 6812 1391
Size: 314 4072
Fragments arranged by size:
4072 1381 934 887 701 699 314 282
207 16
BbeI G_GCGC'C
Cuts at: 6563 6563
Size: 9493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3992 4019 4308 5490 928
Size: 971 2093 27 289 1182 4931
Fragments arranged by size:
4931 2093 1182 971 289 27
BbvCI CC'TCA_GC
Cuts at: 2074 2074
Size: 9493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6147 6181 6331 6365 7684 7718 1903
Size: 34 4210 34 150 34 1319 34 3678
Fragments arranged by size:
4210 3678 1319 150 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3348 7385 8595 3348
Size: 4037 1210 4246
Fragments arranged by size:
4246 4037 1210
BclI T'GATC_A
Cuts at: 1548 3741 4975 1548
Size: 2193 1234 6066
Fragments arranged by size:
6066 2193 1234
BfrBI ATG'CAT
Cuts at: 6438 6438
Size: 9493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3140 6557 8104
Size: 107 122 71 631 605 1468 3417 1547
Cuts at: 8104 136
Size: 1525
Fragments arranged by size:
3417 1547 1525 1468 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2851 4044 9488 2851
Size: 1193 5444 2856
Fragments arranged by size:
5444 2856 1193
BlpI GC'TnA_GC
Cuts at: 5325 6687 6910 5325
Size: 1362 223 7908
Fragments arranged by size:
7908 1362 223
Bme1580I G_kGCm'C
Cuts at: 1790 3136 3533 6072 7554 8710 1790
Size: 1346 397 2539 1482 1156 2573
Fragments arranged by size:
2573 2539 1482 1346 1156 397
BmgBI CAC'GTC
Cuts at: 1318 5842 5887 1318
Size: 4524 45 4924
Fragments arranged by size:
4924 4524 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4139 5127 6125
Size: 416 53 83 399 24 2722 988 998
Cuts at: 6125 6385 7224 8182 442
Size: 260 839 958 1753
Fragments arranged by size:
2722 1753 998 988 958 839 416 399
260 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3448 4558 4675 3448
Size: 1110 117 8266
Fragments arranged by size:
8266 1110 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 3692 3732 4412 4841 4895 5533 5985
Size: 873 1580 40 680 429 54 638 452
Cuts at: 5985 6391 6512 8153 1239
Size: 406 121 1641 2579
Fragments arranged by size:
2579 1641 1580 873 680 638 452 429
406 121 54 40
Bpu10I CC'TnA_GC
Cuts at: 2074 2197 4373 5561 6476 2074
Size: 123 2176 1188 915 5091
Fragments arranged by size:
5091 2176 1188 915 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3178 4004 4787 5776 7618 8483 8781 2786
Size: 392 826 783 989 1842 865 298 3498
Fragments arranged by size:
3498 1842 989 865 826 783 392 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 4088 6405 8156 882
Size: 617 575 2014 2317 1751 2219
Fragments arranged by size:
2317 2219 2014 1751 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 2823 6292 7284 493
Size: 711 1619 3469 992 2702
Fragments arranged by size:
3469 2702 1619 992 711
BsaBI GATnn'nnATC
Cuts at: 2393 2399 3212 9487 2393
Size: 6 813 6275 2399
Fragments arranged by size:
6275 2399 813 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4313 5718 6370 6560
Size: 53 83 186 936 2780 1405 652 190
Cuts at: 6560 7300 7682 8317 275
Size: 740 382 635 1451
Fragments arranged by size:
2780 1451 1405 936 740 652 635 382
190 186 83 53
BsaWI w'CCGG_w
Cuts at: 2609 3204 4917 5315 5487 7926 8324 8598 8745
Size: 595 1713 398 172 2439 398 274 147
Cuts at: 8745 9156 2609
Size: 411 2946
Fragments arranged by size:
2946 2439 1713 595 411 398 398 274
172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3665 3695 5282 5312 1178
Size: 30 165 30 2262 30 1587 30 5359
Fragments arranged by size:
5359 2262 1587 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2192 2195 2483 2976 4604 4892 5920
Size: 876 130 3 288 493 1628 288 1028
Cuts at: 5920 5980 6172 6749 1186
Size: 60 192 577 3930
Fragments arranged by size:
3930 1628 1028 876 577 493 288 288
192 130 60 3
BseYI C'CCAG_C
Cuts at: 2086 2909 3903 4601 4952 5212 5721 6449 6470
Size: 823 994 698 351 260 509 728 21
Cuts at: 6470 8696 9192 2086
Size: 2226 496 2387
Fragments arranged by size:
2387 2226 994 823 728 698 509 496
351 260 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2832 5340 1163
Size: 573 388 708 2508 5316
Fragments arranged by size:
5316 2508 708 573 388
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6943 7704 8732 1540
Size: 275 186 4942 761 1028 2301
Fragments arranged by size:
4942 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3533 4444 4661 5595 7554 7639 8710 3533
Size: 911 217 934 1959 85 1071 4316
Fragments arranged by size:
4316 1959 1071 934 911 217 85
BsmI GAATG_Cn'
Cuts at: 3079 5166 7004 7097 3079
Size: 2087 1838 93 5475
Fragments arranged by size:
5475 2087 1838 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3794 4586 3794
Size: 792 8701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2772 2979 3136 3533 3678 4379 4444
Size: 399 982 207 157 397 145 701 65
Cuts at: 4444 4661 5595 6072 6482 6498 6812 7554 7639
Size: 217 934 477 410 16 314 742 85
Cuts at: 7639 8710 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 982 934 742 701 477 410
399 397 314 217 207 157 145 85
65 16
BspEI T'CCGG_A
Cuts at: 3204 9156 3204
Size: 5952 3541
BspHI T'CATG_A
Cuts at: 3513 3808 5259 7382 9112 3513
Size: 295 1451 2123 1730 3894
Fragments arranged by size:
3894 2123 1730 1451 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5361 6453 6848 844
Size: 4517 1092 395 3489
Fragments arranged by size:
4517 3489 1092 395
BsrBI CCG'CTC
Cuts at: 1394 1823 2762 5569 6686 7380 1394
Size: 429 939 2807 1117 694 3507
Fragments arranged by size:
3507 2807 1117 939 694 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7988 8162 65
Size: 1183 6740 174 1396
Fragments arranged by size:
6740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2609 4743 4755 5654 6421 8137
Size: 110 255 544 2134 12 899 767 1716
Cuts at: 8137 8324 1700
Size: 187 2869
Fragments arranged by size:
2869 2134 1716 899 767 544 255 187
110 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9493
BssSI C'ACGA_G
Cuts at: 4937 5821 7553 8565 4937
Size: 884 1732 1012 5865
Fragments arranged by size:
5865 1732 1012 884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3131 3787 794
Size: 2337 656 6500
Fragments arranged by size:
6500 2337 656
BstBI TT'CG_AA
Cuts at: 5864 8297 5864
Size: 2433 7060
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9493
BstXI CCAn_nnnn'nTGG
Cuts at: 4507 4507
Size: 9493
BstYI r'GATC_y
Cuts at: 1972 2145 2754 2851 3238 3385 4044 5490 6261
Size: 173 609 97 387 147 659 1446 771
Cuts at: 6261 6300 6823 7190 7575 7592 9044 9130 9142
Size: 39 523 367 385 17 1452 86 12
Cuts at: 9142 9488 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1446 771 659 609 523 387
385 367 346 173 147 97 86 39
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2772 3494 3507 513
Size: 552 939 768 722 13 6499
Fragments arranged by size:
6499 939 768 722 552 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4863 487
Size: 683 56 3637 5117
Fragments arranged by size:
5117 3637 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3343 5425 5767 5806 6048 7009 7803 7823
Size: 528 2082 342 39 242 961 794 20
Cuts at: 7823 2815
Size: 4485
Fragments arranged by size:
4485 2082 961 794 528 342 242 39
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5497 5532 529
Size: 35 4933 35 4490
Fragments arranged by size:
4933 4490 35 35
DraI TTT'AAA
Cuts at: 6797 6797
Size: 9493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8500 809
Size: 507 7184 1802
Fragments arranged by size:
7184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3648 4509 4750
Size: 54 1425 50 275 18 1818 861 241
Cuts at: 4750 5656 6522 6940 7829 9458 8
Size: 906 866 418 889 1629 43
Fragments arranged by size:
1818 1629 1425 906 889 866 861 418
275 241 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6940 1537
Size: 275 5128 4090
Fragments arranged by size:
5128 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 4449 4649 6188 6353 6726 7423 1353
Size: 906 2190 200 1539 165 373 697 3423
Fragments arranged by size:
3423 2190 1539 906 697 373 200 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2172 6669 8080 8454 8600 387
Size: 1064 138 583 4497 1411 374 146 1280
Fragments arranged by size:
4497 1411 1280 1064 583 374 146 138
EcoICRI GAG'CTC
Cuts at: 4659 5593 4659
Size: 934 8559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2333 2921 3692 3732 4094 4412
Size: 393 480 221 588 771 40 362 318
Cuts at: 4412 4841 4895 5019 5533 5985 6209 6326 6391
Size: 429 54 124 514 452 224 117 65
Cuts at: 6391 6512 7556 8153 8940 9208 1239
Size: 121 1044 597 787 268 1524
Fragments arranged by size:
1524 1044 787 771 597 588 514 480
452 429 393 362 318 268 224 221
124 121 117 65 54 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 6725 2597
Size: 4128 5365
EcoO109I rG'GnC_Cy
Cuts at: 2132 2556 5360 5499 6679 6784 2132
Size: 424 2804 139 1180 105 4841
Fragments arranged by size:
4841 2804 1180 424 139 105
EcoRI G'AATT_C
Cuts at: 6802 7180 6802
Size: 378 9115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3618 5695 3618
Size: 2077 7416
FspI TGC'GCA
Cuts at: 7999 7999
Size: 9493
HaeII r_GCGC'y
Cuts at: 1678 6563 8640 1678
Size: 4885 2077 2531
Fragments arranged by size:
4885 2531 2077
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2746 2778 3410
Size: 32 135 32 632 32 510 32 632
Cuts at: 3410 3442 3665 3697 4679 4711 5030 5062 5681
Size: 32 223 32 982 32 319 32 619
Cuts at: 5681 5713 5998 6030 6389 6421 8134 8166 8208
Size: 32 285 32 359 32 1713 32 42
Cuts at: 8208 8240 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 982 632 632 619 510 359
319 285 223 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3349 6106 6837 7083 669
Size: 988 281 1411 2757 731 246 3079
Fragments arranged by size:
3079 2757 1411 988 731 281 246
HindIII A'AGCT_T
Cuts at: 748 5664 748
Size: 4916 4577
HpaI GTT'AAC
Cuts at: 3349 7083 3349
Size: 3734 5759
KasI G'GCGC_C
Cuts at: 6559 6559
Size: 9493
KpnI G_GTAC'C
Cuts at: 6818 6818
Size: 9493
MfeI C'AATT_G
Cuts at: 7092 7092
Size: 9493
MluI A'CGCG_T
Cuts at: 9369 9369
Size: 9493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 2247 3266 3387 3548 3940 3957 4317
Size: 936 6 1019 121 161 392 17 360
Cuts at: 4317 4472 4749 5739 6147 8607 8791 1305
Size: 155 277 990 408 2460 184 2007
Fragments arranged by size:
2460 2007 1019 990 936 408 392 360
277 184 161 155 121 17 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3650 4511 4752 6524 10
Size: 54 1425 343 1818 861 241 1772 2979
Fragments arranged by size:
2979 1818 1772 1425 861 343 241 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3512 4528 5174 5309 6077 6518 6545
Size: 648 2346 1016 646 135 768 441 27
Cuts at: 6545 7451 7810 7969 518
Size: 906 359 159 2042
Fragments arranged by size:
2346 2042 1016 906 768 648 646 441
359 159 135 27
MspA1I CmG'CkG
Cuts at: 1606 2090 2688 3496 4611 5599 6484 6909 7586
Size: 484 598 808 1115 988 885 425 677
Cuts at: 7586 8734 8979 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1115 988 885 808 677 598
484 425 245
NaeI GCC'GGC
Cuts at: 5656 5656
Size: 9493
NarI GG'CG_CC
Cuts at: 6560 6560
Size: 9493
NcoI C'CATG_G
Cuts at: 513 1065 2004 3507 513
Size: 552 939 1503 6499
Fragments arranged by size:
6499 1503 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9493
NgoMIV G'CCGG_C
Cuts at: 5654 5654
Size: 9493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9493
NotI GC'GGCC_GC
Cuts at: 6940 6940
Size: 9493
NsiI A_TGCA'T
Cuts at: 6440 6440
Size: 9493
NspI r_CATG'y
Cuts at: 1132 1229 5389 6851 8396 1132
Size: 97 4160 1462 1545 2229
Fragments arranged by size:
4160 2229 1545 1462 97
PasI CC'CwG_GG
Cuts at: 1348 2794 4103 1348
Size: 1446 1309 6738
Fragments arranged by size:
6738 1446 1309
PciI A'CATG_T
Cuts at: 8392 8392
Size: 9493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2135 2726 4864 1108
Size: 1027 591 2138 5737
Fragments arranged by size:
5737 2138 1027 591
PfoI T'CCnGG_A
Cuts at: 2128 2128
Size: 9493
PmeI GTTT'AAAC
Cuts at: 6797 6797
Size: 9493
PmlI CAC'GTG
Cuts at: 2823 6292 2823
Size: 3469 6024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 2135 6047 6079 7562 7594 9075 9107 2103
Size: 32 3912 32 1483 32 1481 32 2489
Fragments arranged by size:
3912 2489 1483 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2132 5360 5499 6784 2132
Size: 3228 139 1285 4841
Fragments arranged by size:
4841 3228 1285 139
PshAI GACnn'nnGTC
Cuts at: 1613 3195 1613
Size: 1582 7911
PsiI TTA'TAA
Cuts at: 7063 7063
Size: 9493
PstI C_TGCA'G
Cuts at: 830 5358 6160 6324 6845 830
Size: 4528 802 164 521 3478
Fragments arranged by size:
4528 3478 802 521 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9493
PvuII CAG'CTG
Cuts at: 2090 2090
Size: 9493
SacI G_AGCT'C
Cuts at: 4661 5595 4661
Size: 934 8559
SacII CC_GC'GG
Cuts at: 3497 3497
Size: 9493
SalI G'TCGA_C
Cuts at: 1936 6835 1936
Size: 4899 4594
SanDI GG'GwC_CC
Cuts at: 5499 5499
Size: 9493
SapI GCTCTTCn'nnn_
Cuts at: 2259 4449 4649 2259
Size: 2190 200 7103
Fragments arranged by size:
7103 2190 200
SbfI CC_TGCA'GG
Cuts at: 5358 6845 5358
Size: 1487 8006
ScaI AGT'ACT
Cuts at: 7741 9453 7741
Size: 1712 7781
SfcI C'TryA_G
Cuts at: 826 1045 5354 6156 6320 6841 7976 8657 8848
Size: 219 4309 802 164 521 1135 681 191
Cuts at: 8848 826
Size: 1471
Fragments arranged by size:
4309 1471 1135 802 681 521 219 191
164
SfoI GGC'GCC
Cuts at: 6561 6561
Size: 9493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9493
SgrAI Cr'CCGG_yG
Cuts at: 1700 2609 1700
Size: 909 8584
SmaI CCC'GGG
Cuts at: 1872 6554 6820 1872
Size: 4682 266 4545
Fragments arranged by size:
4682 4545 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2023 2765 3193 4019 4802 5755
Size: 197 926 80 742 428 826 783 953
Cuts at: 5755 7597 8498 8760 820
Size: 1842 901 262 1553
Fragments arranged by size:
1842 1553 953 926 901 826 783 742
428 262 197 80
SnaBI TAC'GTA
Cuts at: 493 7284 493
Size: 6791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9493
SphI G_CATG'C
Cuts at: 1132 6851 1132
Size: 5719 3774
SspI AAT'ATT
Cuts at: 5 52 7417 5
Size: 47 7365 2081
Fragments arranged by size:
7365 2081 47
StuI AGG'CCT
Cuts at: 1517 4255 5068 1517
Size: 2738 813 5942
Fragments arranged by size:
5942 2738 813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2943 3312 3507 5191 5377 5409
Size: 552 939 939 369 195 1684 186 32
Cuts at: 5409 5494 5739 6932 513
Size: 85 245 1193 3074
Fragments arranged by size:
3074 1684 1193 939 939 552 369 245
195 186 85 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 2923 3520 4350 4513 6404 7520
Size: 1255 653 690 597 830 163 1891 1116
Cuts at: 7520 7537 7690 7875 8320 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1891 1498 1255 1116 830 690 653 597
445 185 163 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5347 7739 9451
Size: 274 80 33 51 1443 3370 2392 1712
Cuts at: 9451 96
Size: 138
Fragments arranged by size:
3370 2392 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 5271 5282 5303 5314 9453 9485 3530
Size: 32 1709 11 21 11 4139 32 3538
Fragments arranged by size:
4139 3538 1709 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9493
XbaI T'CTAG_A
Cuts at: 6829 6829
Size: 9493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3060 3280 6280 3060
Size: 220 3000 6273
Fragments arranged by size:
6273 3000 220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9493
XmaI C'CCGG_G
Cuts at: 1870 6552 6818 1870
Size: 4682 266 4545
Fragments arranged by size:
4682 4545 266
XmnI GAAnn'nnTTC
Cuts at: 2708 6197 7622 2708
Size: 3489 1425 4579
Fragments arranged by size:
4579 3489 1425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7301 276
Size: 53 83 186 936 5767 2468
Fragments arranged by size:
5767 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BsiWI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI NruI PacI PspOMI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI