Restriction map for
pFN21AA0433
(Circular) (Six-base) MAPSORT of: pFN21AA0433.seq Check: 7080 from: 1 to: 8434
pFN21AA0433 8434 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5569 5569
Size: 8434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6244 278
Size: 53 83 186 936 4708 2468
Fragments arranged by size:
4708 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5555 5777 1937
Size: 3618 222 4594
Fragments arranged by size:
4594 3618 222
Acc65I G'GTAC_C
Cuts at: 4967 5755 4967
Size: 788 7646
AclI AA'CG_TT
Cuts at: 1563 4578 6561 6934 1563
Size: 3015 1983 373 3063
Fragments arranged by size:
3063 3015 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2802 2962 3117 3577 3594 3869 3930
Size: 822 348 160 155 460 17 275 61
Cuts at: 3930 4358 6497 7881 8149 1632
Size: 428 2139 1384 268 1917
Fragments arranged by size:
2139 1917 1384 822 460 428 348 275
268 160 155 61 17
AflII C'TTAA_G
Cuts at: 820 1017 3389 820
Size: 197 2372 5865
Fragments arranged by size:
5865 2372 197
AflIII A'CryG_T
Cuts at: 1203 3856 7333 8310 1203
Size: 2653 3477 977 1327
Fragments arranged by size:
3477 2653 1327 977
AgeI A'CCGG_T
Cuts at: 7265 7265
Size: 8434
AhdI GACnn_n'nnGTC
Cuts at: 7163 7163
Size: 8434
AleI CACnn'nnGTG
Cuts at: 1166 5204 1166
Size: 4038 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5222 1184
Size: 4038 4396
AlwNI CAG_nnn'CTG
Cuts at: 6177 7749 6177
Size: 1572 6862
ApaLI G'TGCA_C
Cuts at: 6491 7647 6491
Size: 1156 7278
ApoI r'AATT_y
Cuts at: 1493 2190 2992 3221 4359 4717 5438 5493 5743
Size: 697 802 229 1138 358 721 55 250
Cuts at: 5743 5969 6121 8230 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1138 802 721 697 358 250
229 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 2879 6988 160
Size: 872 1847 4109 1606
Fragments arranged by size:
4109 1847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
AvaI C'yCGr_G
Cuts at: 1870 1943 5210 5759 1870
Size: 73 3267 549 4545
Fragments arranged by size:
4545 3267 549 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2727 2760 2727
Size: 33 8401
BamHI G'GATC_C
Cuts at: 4630 5764 6131 4630
Size: 1134 367 6933
Fragments arranged by size:
6933 1134 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4520 4967 5377 5755 7210
Size: 325 217 627 2733 447 410 378 1455
Cuts at: 7210 618
Size: 1842
Fragments arranged by size:
2733 1842 1455 627 447 410 378 325
217
BanII G_rGCy'C
Cuts at: 1391 4593 4937 5753 1391
Size: 3202 344 816 4072
Fragments arranged by size:
4072 3202 816 344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 3992 4716 4916 5017 5489 928
Size: 971 201 1892 724 200 101 472 3873
Fragments arranged by size:
3873 1892 971 724 472 201 200 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6625 6659 1903
Size: 34 4688 34 3678
Fragments arranged by size:
4688 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3615 6326 7536 3615
Size: 2711 1210 4513
Fragments arranged by size:
4513 2711 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8434
BfrBI ATG'CAT
Cuts at: 3964 3964
Size: 8434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7045 136
Size: 107 122 71 631 605 5373 1525
Fragments arranged by size:
5373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4901 8429 4901
Size: 3528 4906
BlpI GC'TnA_GC
Cuts at: 5851 5851
Size: 8434
Bme1580I G_kGCm'C
Cuts at: 1790 6495 7651 1790
Size: 4705 1156 2573
Fragments arranged by size:
4705 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5010 5730 6165
Size: 416 53 83 399 24 3593 720 435
Cuts at: 6165 7123 442
Size: 958 1753
Fragments arranged by size:
3593 1753 958 720 435 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2658 2781 3630 4340 7094 1239
Size: 1419 123 849 710 2754 2579
Fragments arranged by size:
2754 2579 1419 849 710 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6559 7424 7722 6559
Size: 865 298 7271
Fragments arranged by size:
7271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4765 5181 7097 882
Size: 617 3266 416 1916 2219
Fragments arranged by size:
3266 2219 1916 617 416
BsaAI yAC'GTr
Cuts at: 493 1204 4109 5553 6225 493
Size: 711 2905 1444 672 2702
Fragments arranged by size:
2905 2702 1444 711 672
BsaBI GATnn'nnATC
Cuts at: 3256 4613 8428 3256
Size: 1357 3815 3262
Fragments arranged by size:
3815 3262 1357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5005 5295 6241 6623
Size: 53 83 186 936 3472 290 946 382
Cuts at: 6623 7258 275
Size: 635 1451
Fragments arranged by size:
3472 1451 946 936 635 382 290 186
83 53
BsaWI w'CCGG_w
Cuts at: 6867 7265 7539 7686 8097 6867
Size: 398 274 147 411 7204
Fragments arranged by size:
7204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8209
Fragments arranged by size:
8209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2258 3503 4335 1186
Size: 931 141 1245 832 5285
Fragments arranged by size:
5285 1245 931 832 141
BseYI C'CCAG_C
Cuts at: 7637 8133 7637
Size: 496 7938
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2595 5664 1163
Size: 573 859 3069 3933
Fragments arranged by size:
3933 3069 859 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5884 6645 7673 1540
Size: 275 186 3883 761 1028 2301
Fragments arranged by size:
3883 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4593 5211 5603 6495 6580 7651 4593
Size: 618 392 892 85 1071 5376
Fragments arranged by size:
5376 1071 892 618 392 85
BsmI GAATG_Cn'
Cuts at: 5945 6038 5945
Size: 93 8341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4593 4937 5211 5603 5753 6495 6580
Size: 399 2803 344 274 392 150 742 85
Cuts at: 6580 7651 1391
Size: 1071 2174
Fragments arranged by size:
2803 2174 1071 742 399 392 344 274
150 85
BspEI T'CCGG_A
Cuts at: 8097 8097
Size: 8434
BspHI T'CATG_A
Cuts at: 3874 6323 8053 3874
Size: 2449 1730 4255
Fragments arranged by size:
4255 2449 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5133 5569 5789 844
Size: 4289 436 220 3489
Fragments arranged by size:
4289 3489 436 220
BsrBI CCG'CTC
Cuts at: 1394 1823 6321 1394
Size: 429 4498 3507
Fragments arranged by size:
4498 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 4537 5451 6929 7103 65
Size: 1183 1441 1848 914 1478 174 1396
Fragments arranged by size:
1848 1478 1441 1396 1183 914 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7078 7265 1700
Size: 110 5268 187 2869
Fragments arranged by size:
5268 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4489 96
Size: 4393 4041
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8434
BssSI C'ACGA_G
Cuts at: 2868 6494 7506 2868
Size: 3626 1012 3796
Fragments arranged by size:
3796 3626 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8434
BstBI TT'CG_AA
Cuts at: 4259 7238 4259
Size: 2979 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8434
BstXI CCAn_nnnn'nTGG
Cuts at: 4214 4993 5062 4214
Size: 779 69 7586
Fragments arranged by size:
7586 779 69
BstYI r'GATC_y
Cuts at: 1972 2202 4051 4630 4642 4901 5568 5764 6131
Size: 230 1849 579 12 259 667 196 367
Cuts at: 6131 6516 6533 7985 8071 8083 8429 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1849 1452 667 579 385 367 346
259 230 196 86 17 12 12
BstZ17I GTA'TAC
Cuts at: 5556 5556
Size: 8434
Bsu36I CC'TnA_GG
Cuts at: 4287 4287
Size: 8434
BtgI C'CryG_G
Cuts at: 513 1065 2154 3074 5373 5477 513
Size: 552 1089 920 2299 104 3470
Fragments arranged by size:
3470 2299 1089 920 552 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7695
Fragments arranged by size:
7695 683 56
BtsI GCAGTG_nn'
Cuts at: 5950 6744 6764 5950
Size: 794 20 7620
Fragments arranged by size:
7620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8399
DraI TTT'AAA
Cuts at: 2215 4138 4575 4780 5424 5547 5738 2215
Size: 1923 437 205 644 123 191 4911
Fragments arranged by size:
4911 1923 644 437 205 191 123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7441 809
Size: 507 6125 1802
Fragments arranged by size:
6125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5881 6770 8399
Size: 54 1425 50 275 18 4051 889 1629
Cuts at: 8399 8
Size: 43
Fragments arranged by size:
4051 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5881 1537
Size: 275 4069 4090
Fragments arranged by size:
4090 4069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2709 3480 3496 3580 4923 5281 5409
Size: 882 474 771 16 84 1343 358 128
Cuts at: 5409 6364 1353
Size: 955 3423
Fragments arranged by size:
3423 1343 955 882 771 474 358 128
84 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7021 7395 7541 387
Size: 1064 138 5432 374 146 1280
Fragments arranged by size:
5432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4591 4591
Size: 8434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2658 2781 2802 2962 3117 3577
Size: 393 822 204 123 21 160 155 460
Cuts at: 3577 3594 3630 3869 3930 4340 4358 6497 7094
Size: 17 36 239 61 410 18 2139 597
Cuts at: 7094 7881 8149 1239
Size: 787 268 1524
Fragments arranged by size:
2139 1524 822 787 597 460 410 393
268 239 204 160 155 123 61 36
21 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 5169 5169
Size: 8434
EcoRI G'AATT_C
Cuts at: 5743 6121 5743
Size: 378 8056
EcoRV GAT'ATC
Cuts at: 3103 3103
Size: 8434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2915 3782 5652 2915
Size: 867 1870 5697
Fragments arranged by size:
5697 1870 867
FspI TGC'GCA
Cuts at: 6940 6940
Size: 8434
HaeII r_GCGC'y
Cuts at: 1678 7581 1678
Size: 5903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3397 3429 3666 3698 3818
Size: 32 135 32 1825 32 237 32 120
Cuts at: 3818 3832 3850 3864 4043 4075 4634 4666 7075
Size: 14 18 14 179 32 559 32 2409
Cuts at: 7075 7107 7149 7181 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2409 1825 559 237 179 135 120
42 32 32 32 32 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 5778 6024 669
Size: 988 281 1509 2331 246 3079
Fragments arranged by size:
3079 2331 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3923 748
Size: 3175 5259
HpaI GTT'AAC
Cuts at: 6024 6024
Size: 8434
KpnI G_GTAC'C
Cuts at: 4971 5759 4971
Size: 788 7646
MfeI C'AATT_G
Cuts at: 3108 6033 3108
Size: 2925 5509
MluI A'CGCG_T
Cuts at: 8310 8310
Size: 8434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2011 2122 7548 7732 1305
Size: 706 111 5426 184 2007
Fragments arranged by size:
5426 2007 706 184 111
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6612
Fragments arranged by size:
6612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2088 5204 6392 6751 6910 518
Size: 648 922 3116 1188 359 159 2042
Fragments arranged by size:
3116 2042 1188 922 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3728 5489 5850 6527 7675 7920 1606
Size: 2122 1761 361 677 1148 245 2120
Fragments arranged by size:
2122 2120 1761 1148 677 361 245
NcoI C'CATG_G
Cuts at: 513 1065 2154 3074 5373 513
Size: 552 1089 920 2299 3574
Fragments arranged by size:
3574 2299 1089 920 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8434
NotI GC'GGCC_GC
Cuts at: 5881 5881
Size: 8434
NsiI A_TGCA'T
Cuts at: 3966 3966
Size: 8434
NspI r_CATG'y
Cuts at: 1132 1229 5792 7337 1132
Size: 97 4563 1545 2229
Fragments arranged by size:
4563 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 3972 3972
Size: 8434
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8434
PciI A'CATG_T
Cuts at: 7333 7333
Size: 8434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5354 1108
Size: 4246 4188
PfoI T'CCnGG_A
Cuts at: 2056 3607 4687 2056
Size: 1551 1080 5803
Fragments arranged by size:
5803 1551 1080
PmeI GTTT'AAAC
Cuts at: 5738 5738
Size: 8434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6503 6535 8016 8048 6503
Size: 32 1481 32 6889
Fragments arranged by size:
6889 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8434
PsiI TTA'TAA
Cuts at: 6004 6004
Size: 8434
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3501 3533 3501
Size: 32 8402
PstI C_TGCA'G
Cuts at: 830 3559 3727 5786 830
Size: 2729 168 2059 3478
Fragments arranged by size:
3478 2729 2059 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8434
PvuII CAG'CTG
Cuts at: 3728 3728
Size: 8434
SacI G_AGCT'C
Cuts at: 4593 4593
Size: 8434
SalI G'TCGA_C
Cuts at: 1936 5776 1936
Size: 3840 4594
SapI GCTCTTCn'nnn_
Cuts at: 3580 3580
Size: 8434
SbfI CC_TGCA'GG
Cuts at: 3559 5786 3559
Size: 2227 6207
ScaI AGT'ACT
Cuts at: 6682 8394 6682
Size: 1712 6722
SfcI C'TryA_G
Cuts at: 826 1045 3555 3670 3723 5169 5237 5502 5782
Size: 219 2510 115 53 1446 68 265 280
Cuts at: 5782 6917 7598 7789 826
Size: 1135 681 191 1471
Fragments arranged by size:
2510 1471 1446 1135 681 280 265 219
191 115 68 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8434
SmaI CCC'GGG
Cuts at: 1872 5761 1872
Size: 3889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3389 6538 7439 7701 820
Size: 197 926 1446 3149 901 262 1553
Fragments arranged by size:
3149 1553 1446 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4109 5553 6225 493
Size: 3616 1444 672 2702
Fragments arranged by size:
3616 2702 1444 672
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8434
SphI G_CATG'C
Cuts at: 1132 5792 1132
Size: 4660 3774
SspI AAT'ATT
Cuts at: 5 52 3037 6358 5
Size: 47 2985 3321 2081
Fragments arranged by size:
3321 2985 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8434
StyI C'CwwG_G
Cuts at: 513 1065 2154 3074 5373 5873 513
Size: 552 1089 920 2299 500 3074
Fragments arranged by size:
3074 2299 1089 920 552 500
SwaI ATTT'AAAT
Cuts at: 2215 2215
Size: 8434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3787 5361 6461 6478 6631 6816 7261
Size: 1255 2207 1574 1100 17 153 185 445
Cuts at: 7261 325
Size: 1498
Fragments arranged by size:
2207 1574 1498 1255 1100 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4340 4489 6680
Size: 274 80 33 51 1443 2363 149 2191
Cuts at: 6680 8392 96
Size: 1712 138
Fragments arranged by size:
2363 2191 1712 1443 274 149 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4535 4567 8394 8426 4535
Size: 32 3827 32 4543
Fragments arranged by size:
4543 3827 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8434
XbaI T'CTAG_A
Cuts at: 4424 5395 5770 4424
Size: 971 375 7088
Fragments arranged by size:
7088 971 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2271 2271
Size: 8434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8434
XmaI C'CCGG_G
Cuts at: 1870 5759 1870
Size: 3889 4545
XmnI GAAnn'nnTTC
Cuts at: 2149 2541 3882 3943 4662 5403 6563 2149
Size: 392 1341 61 719 741 1160 4020
Fragments arranged by size:
4020 1341 1160 741 719 392 61
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6242 276
Size: 53 83 186 936 4708 2468
Fragments arranged by size:
4708 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI DraIII EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SfoI SrfI