Restriction map for
pFN21AA0432
(Circular) (Six-base) MAPSORT of: pFN21AA0432.seq Check: 7756 from: 1 to: 7111
pFN21AA0432 7111 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2548 4921 278
Size: 53 83 186 936 1012 2373 2468
Fragments arranged by size:
2468 2373 1012 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4454 1937
Size: 2517 4594
Acc65I G'GTAC_C
Cuts at: 3821 4432 3821
Size: 611 6500
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2484 3285 5174 6558 6826 1632
Size: 852 801 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 852 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 6010 6987 1203
Size: 4807 977 1327
Fragments arranged by size:
4807 1327 977
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 5840 5840
Size: 7111
AleI CACnn'nnGTG
Cuts at: 1166 3125 1166
Size: 1959 5152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2155 2305 1184
Size: 971 150 5990
Fragments arranged by size:
5990 971 150
AlwNI CAG_nnn'CTG
Cuts at: 4854 6426 4854
Size: 1572 5539
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 2055 2306 2595 2864 2995 3087 4158 4406
Size: 562 251 289 269 131 92 1071 248
Cuts at: 4406 4420 4646 4798 6907 1493
Size: 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 562 289 269 251 248
226 152 131 92 14
AseI AT'TA_AT
Cuts at: 160 1032 3354 5665 160
Size: 872 2322 2311 1606
Fragments arranged by size:
2322 2311 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 3359 3420 4091 4436 1870
Size: 73 1416 61 671 345 4545
Fragments arranged by size:
4545 1416 671 345 73 61
BamHI G'GATC_C
Cuts at: 2167 4441 4808 2167
Size: 2274 367 4470
Fragments arranged by size:
4470 2274 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3821 4432 5887 618
Size: 325 217 627 2034 611 1455 1842
Fragments arranged by size:
2034 1842 1455 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2476 3960 4197 4430 1391
Size: 1085 1484 237 233 4072
Fragments arranged by size:
4072 1484 1085 237 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2189 4326 928
Size: 971 290 2137 3713
Fragments arranged by size:
3713 2137 971 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5302 5336 1903
Size: 34 50 34 3281 34 3678
Fragments arranged by size:
3678 3281 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5003 6213 5003
Size: 1210 5901
BclI T'GATC_A
Cuts at: 1548 2019 3741 1548
Size: 471 1722 4918
Fragments arranged by size:
4918 1722 471
BfrBI ATG'CAT
Cuts at: 3616 3616
Size: 7111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2480 4049 4130
Size: 107 122 71 631 605 808 1569 81
Cuts at: 4130 5722 136
Size: 1592 1525
Fragments arranged by size:
1592 1569 1525 808 631 605 122 107
81 71
BglII A'GATC_T
Cuts at: 2759 3634 7106 2759
Size: 875 3472 2764
Fragments arranged by size:
3472 2764 875
BlpI GC'TnA_GC
Cuts at: 4528 4528
Size: 7111
Bme1580I G_kGCm'C
Cuts at: 1790 5172 6328 1790
Size: 3382 1156 2573
Fragments arranged by size:
3382 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2966 1318
Size: 1648 5463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2281 4842 5800
Size: 416 53 83 399 24 864 2561 958
Cuts at: 5800 442
Size: 1753
Fragments arranged by size:
2561 1753 958 864 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3446
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2909 4403 5771 1239
Size: 1161 509 1494 1368 2579
Fragments arranged by size:
2579 1494 1368 1161 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 3946 4253 5236 6101 6399 2471
Size: 1475 307 983 865 298 3183
Fragments arranged by size:
3183 1475 983 865 307 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5774 882
Size: 617 4275 2219
Fragments arranged by size:
4275 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3397 4902 493
Size: 711 2193 1505 2702
Fragments arranged by size:
2702 2193 1505 711
BsaBI GATnn'nnATC
Cuts at: 2394 7105 2394
Size: 4711 2400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2545 4918 5300 5935
Size: 53 83 186 936 1012 2373 382 635
Cuts at: 5935 275
Size: 1451
Fragments arranged by size:
2373 1451 1012 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5544 5942 6216 6363 6774 5544
Size: 398 274 147 411 5881
Fragments arranged by size:
5881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2245 2275 1178
Size: 30 165 30 842 30 6014
Fragments arranged by size:
6014 842 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2637 3321 1186
Size: 1451 684 4976
Fragments arranged by size:
4976 1451 684
BseYI C'CCAG_C
Cuts at: 6314 6810 6314
Size: 496 6615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3935 1163
Size: 573 2199 4339
Fragments arranged by size:
4339 2199 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4561 5322 6350 1540
Size: 275 186 2560 761 1028 2301
Fragments arranged by size:
2560 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3840 3960 5172 5257 6328 3840
Size: 120 1212 85 1071 4623
Fragments arranged by size:
4623 1212 1071 120 85
BsmI GAATG_Cn'
Cuts at: 3618 3998 4622 4715 3618
Size: 380 624 93 6014
Fragments arranged by size:
6014 624 380 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2476 3840 3960 4197 4430 5172 5257
Size: 399 686 1364 120 237 233 742 85
Cuts at: 5257 6328 1391
Size: 1071 2174
Fragments arranged by size:
2174 1364 1071 742 686 399 237 233
120 85
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 4197 5000 6730 4197
Size: 803 1730 4578
Fragments arranged by size:
4578 1730 803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4466 844
Size: 3622 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4998 1394
Size: 429 3175 3507
Fragments arranged by size:
3507 3175 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2111 2256 3161 5606 5780 65
Size: 1183 863 145 905 2445 174 1396
Fragments arranged by size:
2445 1396 1183 905 863 174 145
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3208 96
Size: 3112 3999
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 4182 5171 6183 4182
Size: 989 1012 5110
Fragments arranged by size:
5110 1012 989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7111
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 4172 1184
Size: 2988 4123
BstXI CCAn_nnnn'nTGG
Cuts at: 2247 2247
Size: 7111
BstYI r'GATC_y
Cuts at: 1972 2167 2389 2740 2759 3634 4441 4808 5193
Size: 195 222 351 19 875 807 367 385
Cuts at: 5193 5210 6662 6748 6760 7106 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 875 807 385 367 351 346
222 195 86 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3949 513
Size: 552 2884 3675
Fragments arranged by size:
3675 2884 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6372
Fragments arranged by size:
6372 683 56
BtsI GCAGTG_nn'
Cuts at: 2144 4627 5421 5441 2144
Size: 2483 794 20 3814
Fragments arranged by size:
3814 2483 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4398 4415 4398
Size: 17 7094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2191 4399 6118 809
Size: 507 875 2208 1719 1802
Fragments arranged by size:
2208 1802 1719 875 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 4558 5447
Size: 54 1425 50 275 18 396 2332 889
Cuts at: 5447 7076 8
Size: 1629 43
Fragments arranged by size:
2332 1629 1425 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2337 2563 2612 2770 3339 3879 5041
Size: 846 138 226 49 158 569 540 1162
Cuts at: 5041 1353
Size: 3423
Fragments arranged by size:
3423 1162 846 569 540 226 158 138
49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5698 6072 6218 387
Size: 1064 138 4109 374 146 1280
Fragments arranged by size:
4109 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3958 3958
Size: 7111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2484 2909 3285 4403 5174 5771
Size: 393 768 84 425 376 1118 771 597
Cuts at: 5771 6558 6826 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1118 787 771 768 597 425 393
376 268 84
EcoRI G'AATT_C
Cuts at: 3087 4420 4798 3087
Size: 1333 378 5400
Fragments arranged by size:
5400 1333 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3167 3987 3167
Size: 820 6291
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 6258 1678
Size: 4580 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2245 2259 2277 2744
Size: 32 135 32 655 18 14 18 467
Cuts at: 2744 2776 2816 2848 5752 5784 5826 5858 1373
Size: 32 40 32 2904 32 42 32 2626
Fragments arranged by size:
2904 2626 655 467 135 42 40 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4455 4701 669
Size: 988 281 2517 246 3079
Fragments arranged by size:
3079 2517 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3969 748
Size: 3221 3890
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KpnI G_GTAC'C
Cuts at: 3825 4436 3825
Size: 611 6500
MfeI C'AATT_G
Cuts at: 2427 2528 4710 2427
Size: 101 2182 4828
Fragments arranged by size:
4828 2182 101
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6225 6409 1305
Size: 4920 184 2007
Fragments arranged by size:
4920 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 10
Size: 54 1425 343 396 4893
Fragments arranged by size:
4893 1425 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3125 3171 3777 4365 5069 5428 5587
Size: 648 1959 46 606 588 704 359 159
Cuts at: 5587 518
Size: 2042
Fragments arranged by size:
2042 1959 704 648 606 588 359 159
46
MspA1I CmG'CkG
Cuts at: 1606 2069 2585 4527 5204 6352 6597 1606
Size: 463 516 1942 677 1148 245 2120
Fragments arranged by size:
2120 1942 1148 677 516 463 245
NcoI C'CATG_G
Cuts at: 513 1065 3949 513
Size: 552 2884 3675
Fragments arranged by size:
3675 2884 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NsiI A_TGCA'T
Cuts at: 3618 3618
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 4469 6014 1132
Size: 97 3240 1545 2229
Fragments arranged by size:
3240 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2270 1108
Size: 1162 5949
PfoI T'CCnGG_A
Cuts at: 2925 2925
Size: 7111
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 2634 3975 4681 2634
Size: 1341 706 5064
Fragments arranged by size:
5064 1341 706
PstI C_TGCA'G
Cuts at: 830 3096 4463 830
Size: 2266 1367 3478
Fragments arranged by size:
3478 2266 1367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 2585 2585
Size: 7111
SacI G_AGCT'C
Cuts at: 3960 3960
Size: 7111
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SapI GCTCTTCn'nnn_
Cuts at: 3879 3879
Size: 7111
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 2892 5359 7071 2892
Size: 2467 1712 2932
Fragments arranged by size:
2932 2467 1712
SexAI A'CCwGG_T
Cuts at: 3978 3978
Size: 7111
SfcI C'TryA_G
Cuts at: 826 1045 3092 3379 3810 3997 4459 5594 6275
Size: 219 2047 287 431 187 462 1135 681
Cuts at: 6275 6466 826
Size: 191 1471
Fragments arranged by size:
2047 1471 1135 681 462 431 287 219
191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 4438 1872
Size: 2566 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 3961 4091 4268 5215 6116
Size: 197 926 507 1511 130 177 947 901
Cuts at: 6116 6378 820
Size: 262 1553
Fragments arranged by size:
1553 1511 947 926 901 507 262 197
177 130
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 2849 2887 152
Size: 2697 38 4376
Fragments arranged by size:
4376 2697 38
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SspI AAT'ATT
Cuts at: 5 52 5035 5
Size: 47 4983 2081
Fragments arranged by size:
4983 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7111
StyI C'CwwG_G
Cuts at: 513 1065 2668 3949 4550 513
Size: 552 1603 1281 601 3074
Fragments arranged by size:
3074 1603 1281 601 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5138 5155 5308 5493 5938 325
Size: 1255 3558 17 153 185 445 1498
Fragments arranged by size:
3558 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2890 3008 3024
Size: 274 80 33 51 1443 913 118 16
Cuts at: 3024 3208 5357 7069 96
Size: 184 2149 1712 138
Fragments arranged by size:
2149 1712 1443 913 274 184 138 118
80 51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7071 7103 7071
Size: 32 7079
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 4447 4447
Size: 7111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 4091 1943
Size: 2148 4963
XmaI C'CCGG_G
Cuts at: 1870 4436 1870
Size: 2566 4545
XmnI GAAnn'nnTTC
Cuts at: 2310 3480 3666 4323 5240 2310
Size: 1170 186 657 917 4181
Fragments arranged by size:
4181 1170 917 657 186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2546 4919 276
Size: 53 83 186 936 1012 2373 2468
Fragments arranged by size:
2468 2373 1012 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI