Restriction map for
pFN21AA0431
(Circular) (Six-base) MAPSORT of: pFN21AA0431.seq Check: 1647 from: 1 to: 6706
pFN21AA0431 6706 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4516 278
Size: 53 83 186 936 2980 2468
Fragments arranged by size:
2980 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4049 1937
Size: 2112 4594
Acc65I G'GTAC_C
Cuts at: 4027 4027
Size: 6706
AclI AA'CG_TT
Cuts at: 1563 4833 5206 1563
Size: 3270 373 3063
Fragments arranged by size:
3270 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 3510 3783 4769 6153 6421 1632
Size: 675 1203 273 986 1384 268 1917
Fragments arranged by size:
1917 1384 1203 986 675 273 268
AflII C'TTAA_G
Cuts at: 820 1017 2621 820
Size: 197 1604 4905
Fragments arranged by size:
4905 1604 197
AflIII A'CryG_T
Cuts at: 1203 2739 2741 5605 6582 1203
Size: 1536 2 2864 977 1327
Fragments arranged by size:
2864 1536 1327 977 2
AgeI A'CCGG_T
Cuts at: 5537 5537
Size: 6706
AhdI GACnn_n'nnGTC
Cuts at: 5435 5435
Size: 6706
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6706
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6706
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 6674
AlwNI CAG_nnn'CTG
Cuts at: 2555 4449 6021 2555
Size: 1894 1572 3240
Fragments arranged by size:
3240 1894 1572
ApaLI G'TGCA_C
Cuts at: 2113 2235 2558 3243 4763 5919 2113
Size: 122 323 685 1520 1156 2900
Fragments arranged by size:
2900 1520 1156 685 323 122
ApoI r'AATT_y
Cuts at: 1493 3235 3607 4015 4241 4393 6502 1493
Size: 1742 372 408 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 408 372 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5260 160
Size: 872 4228 1606
Fragments arranged by size:
4228 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6706
AvaI C'yCGr_G
Cuts at: 1870 1943 4031 1870
Size: 73 2088 4545
Fragments arranged by size:
4545 2088 73
BamHI G'GATC_C
Cuts at: 4036 4403 4036
Size: 367 6339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4027 5482 618
Size: 325 217 627 2240 1455 1842
Fragments arranged by size:
2240 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2543 4025 1391
Size: 1152 1482 4072
Fragments arranged by size:
4072 1482 1152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 928
Size: 971 459 5276
Fragments arranged by size:
5276 971 459
BbvCI CC'TCA_GC
Cuts at: 2506 2506
Size: 6706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2168 2202 4897 4931 1903
Size: 34 231 34 2695 34 3678
Fragments arranged by size:
3678 2695 231 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4598 5808 4598
Size: 1210 5496
BclI T'GATC_A
Cuts at: 1548 2533 1548
Size: 985 5721
BfrBI ATG'CAT
Cuts at: 2014 2969 2014
Size: 955 5751
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2552 5317 136
Size: 107 122 71 631 605 880 2765 1525
Fragments arranged by size:
2765 1525 880 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2782 6701 2782
Size: 3919 2787
BlpI GC'TnA_GC
Cuts at: 4123 4123
Size: 6706
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2178 2239 2562 3247 4767 5923 1790
Size: 327 61 61 323 685 1520 1156 2573
Fragments arranged by size:
2573 1520 1156 685 327 323 61 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2181 4437 5395
Size: 416 53 83 399 24 764 2256 958
Cuts at: 5395 442
Size: 1753
Fragments arranged by size:
2256 1753 958 764 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6706
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2424 2632 2652 2424
Size: 208 20 6478
Fragments arranged by size:
6478 208 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2989 3700 5366 1239
Size: 1750 711 1666 2579
Fragments arranged by size:
2579 1750 1666 711
Bpu10I CC'TnA_GC
Cuts at: 2506 2506
Size: 6706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 2868 3647 4831 5696 5994 2292
Size: 576 779 1184 865 298 3004
Fragments arranged by size:
3004 1184 865 779 576 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3007 3746 5369 882
Size: 617 1508 739 1623 2219
Fragments arranged by size:
2219 1623 1508 739 617
BsaAI yAC'GTr
Cuts at: 493 1204 2742 4497 493
Size: 711 1538 1755 2702
Fragments arranged by size:
2702 1755 1538 711
BsaBI GATnn'nnATC
Cuts at: 6700 6700
Size: 6706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4513 4895 5530 275
Size: 53 83 186 936 2980 382 635 1451
Fragments arranged by size:
2980 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2109 5139 5537 5811 5958 6369 2109
Size: 3030 398 274 147 411 2446
Fragments arranged by size:
3030 2446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3935 3965 3980 4010 1178
Size: 30 165 30 2532 30 15 30 3874
Fragments arranged by size:
3874 2532 165 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2494 1186
Size: 1308 5398
BseYI C'CCAG_C
Cuts at: 2511 2927 3402 3418 5909 6405 2511
Size: 416 475 16 2491 496 2812
Fragments arranged by size:
2812 2491 496 475 416 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2795 2802 3207 1163
Size: 573 329 730 7 405 4662
Fragments arranged by size:
4662 730 573 405 329 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4156 4917 5945 1540
Size: 275 186 2155 761 1028 2301
Fragments arranged by size:
2301 2155 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2239 2562 3247 4767 4852 5923 2117
Size: 122 323 685 1520 85 1071 2900
Fragments arranged by size:
2900 1520 1071 685 323 122 85
BsiWI C'GTAC_G
Cuts at: 2055 2055
Size: 6706
BsmI GAATG_Cn'
Cuts at: 4217 4310 4217
Size: 93 6613
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2178 2239 2543 2562 3247 4025
Size: 399 327 61 61 304 19 685 778
Cuts at: 4025 4767 4852 5923 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 685 399 327 304
85 61 61 19
BspEI T'CCGG_A
Cuts at: 6369 6369
Size: 6706
BspHI T'CATG_A
Cuts at: 4595 6325 4595
Size: 1730 4976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 3799 4061 844
Size: 1350 1605 262 3489
Fragments arranged by size:
3489 1605 1350 262
BsrBI CCG'CTC
Cuts at: 1394 1823 4593 1394
Size: 429 2770 3507
Fragments arranged by size:
3507 2770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3906 5201 5375 65
Size: 1183 2658 1295 174 1396
Fragments arranged by size:
2658 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5350 5537 1700
Size: 110 3540 187 2869
Fragments arranged by size:
3540 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3052 96
Size: 2956 3750
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6706
BssSI C'ACGA_G
Cuts at: 2177 4766 5778 2177
Size: 2589 1012 3105
Fragments arranged by size:
3105 2589 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 794
Size: 1772 4934
BstBI TT'CG_AA
Cuts at: 5510 5510
Size: 6706
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6706
BstXI CCAn_nnnn'nTGG
Cuts at: 2996 2996
Size: 6706
BstYI r'GATC_y
Cuts at: 1972 2782 4036 4403 4788 4805 6257 6343 6355
Size: 810 1254 367 385 17 1452 86 12
Cuts at: 6355 6701 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1254 810 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5967
Fragments arranged by size:
5967 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 2829 3774 4222 5016 5036 2147
Size: 682 945 448 794 20 3817
Fragments arranged by size:
3817 945 794 682 448 20
ClaI AT'CG_AT
Cuts at: 1554 3438 1554
Size: 1884 4822
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6671
DraI TTT'AAA
Cuts at: 3201 4010 3201
Size: 809 5897
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2785 5713 809
Size: 507 1469 2928 1802
Fragments arranged by size:
2928 1802 1469 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4153 5042 6671
Size: 54 1425 50 275 18 2323 889 1629
Cuts at: 6671 8
Size: 43
Fragments arranged by size:
2323 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4153 1537
Size: 275 2341 4090
Fragments arranged by size:
4090 2341 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2898 3447 3486 4636 1353
Size: 1545 549 39 1150 3423
Fragments arranged by size:
3423 1545 1150 549 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5293 5667 5813 387
Size: 1064 138 3704 374 146 1280
Fragments arranged by size:
3704 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2989 3510 3700 3783 4769 5366
Size: 393 675 682 521 190 83 986 597
Cuts at: 5366 6153 6421 1239
Size: 787 268 1524
Fragments arranged by size:
1524 986 787 682 675 597 521 393
268 190 83
EcoO109I rG'GnC_Cy
Cuts at: 2071 2200 2071
Size: 129 6577
EcoRI G'AATT_C
Cuts at: 4015 4393 4015
Size: 378 6328
EcoRV GAT'ATC
Cuts at: 2405 3448 2405
Size: 1043 5663
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2909 2909
Size: 6706
FspI TGC'GCA
Cuts at: 5212 5212
Size: 6706
HaeII r_GCGC'y
Cuts at: 1678 5853 1678
Size: 4175 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2525 2557 3019 3051 3148
Size: 32 135 32 953 32 462 32 97
Cuts at: 3148 3180 3187 3219 3268 3300 3343 3375 3931
Size: 32 7 32 49 32 43 32 556
Cuts at: 3931 3963 5347 5379 5421 5453 1373
Size: 32 1384 32 42 32 2626
Fragments arranged by size:
2626 1384 953 556 462 135 97 49
43 42 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4050 4296 669
Size: 988 281 2112 246 3079
Fragments arranged by size:
3079 2112 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2319 748
Size: 1571 5135
HpaI GTT'AAC
Cuts at: 4296 4296
Size: 6706
KpnI G_GTAC'C
Cuts at: 4031 4031
Size: 6706
MfeI C'AATT_G
Cuts at: 4305 4305
Size: 6706
MluI A'CGCG_T
Cuts at: 6582 6582
Size: 6706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 3062 4023 5820 6004 1305
Size: 975 782 961 1797 184 2007
Fragments arranged by size:
2007 1797 975 961 782 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4884
Fragments arranged by size:
4884 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4664 5023 5182 518
Size: 648 843 2655 359 159 2042
Fragments arranged by size:
2655 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4122 4799 5947 6192 1606
Size: 2516 677 1148 245 2120
Fragments arranged by size:
2516 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6154
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6706
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6706
NotI GC'GGCC_GC
Cuts at: 4153 4153
Size: 6706
NsiI A_TGCA'T
Cuts at: 2016 2971 2016
Size: 955 5751
NspI r_CATG'y
Cuts at: 1132 1229 2018 2087 2121 4064 5609 1132
Size: 97 789 69 34 1943 1545 2229
Fragments arranged by size:
2229 1943 1545 789 97 69 34
PasI CC'CwG_GG
Cuts at: 1348 3859 1348
Size: 2511 4195
PciI A'CATG_T
Cuts at: 5605 5605
Size: 6706
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 3355 1108
Size: 1410 837 4459
Fragments arranged by size:
4459 1410 837
PfoI T'CCnGG_A
Cuts at: 3388 3388
Size: 6706
PmeI GTTT'AAAC
Cuts at: 4010 4010
Size: 6706
PmlI CAC'GTG
Cuts at: 2742 2742
Size: 6706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 4775 4807 6288 6320 3935
Size: 32 808 32 1481 32 4321
Fragments arranged by size:
4321 1481 808 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2200 2071
Size: 129 6577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6706
PsiI TTA'TAA
Cuts at: 3134 3233 4276 3134
Size: 99 1043 5564
Fragments arranged by size:
5564 1043 99
PstI C_TGCA'G
Cuts at: 830 2154 2191 4058 830
Size: 1324 37 1867 3478
Fragments arranged by size:
3478 1867 1324 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6706
SalI G'TCGA_C
Cuts at: 1936 4048 1936
Size: 2112 4594
SanDI GG'GwC_CC
Cuts at: 2200 2200
Size: 6706
SbfI CC_TGCA'GG
Cuts at: 4058 4058
Size: 6706
ScaI AGT'ACT
Cuts at: 2680 3733 4954 6666 2680
Size: 1053 1221 1712 2720
Fragments arranged by size:
2720 1712 1221 1053
SfcI C'TryA_G
Cuts at: 826 1045 2150 2187 2493 2949 3120 3960 4054
Size: 219 1105 37 306 456 171 840 94
Cuts at: 4054 5189 5870 6061 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 840 681 456 306 219
191 171 94 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6706
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6706
SmaI CCC'GGG
Cuts at: 1872 4033 1872
Size: 2161 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 2621 2883 3626 4810 5711
Size: 197 926 364 314 262 743 1184 901
Cuts at: 5711 5973 820
Size: 262 1553
Fragments arranged by size:
1553 1184 926 901 743 364 314 262
262 197
SnaBI TAC'GTA
Cuts at: 493 4497 493
Size: 4004 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6706
SphI G_CATG'C
Cuts at: 1132 2018 4064 1132
Size: 886 2046 3774
Fragments arranged by size:
3774 2046 886
SspI AAT'ATT
Cuts at: 5 52 4630 5
Size: 47 4578 2081
Fragments arranged by size:
4578 2081 47
StuI AGG'CCT
Cuts at: 1517 3624 1517
Size: 2107 4599
StyI C'CwwG_G
Cuts at: 513 1065 4145 513
Size: 552 3080 3074
Fragments arranged by size:
3080 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3585 4733 4750 4903 5088 5533
Size: 1255 1836 169 1148 17 153 185 445
Cuts at: 5533 325
Size: 1498
Fragments arranged by size:
1836 1498 1255 1148 445 185 169 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2678 3052 3731
Size: 274 80 33 51 1443 701 374 679
Cuts at: 3731 3821 4952 6664 96
Size: 90 1131 1712 138
Fragments arranged by size:
1712 1443 1131 701 679 374 274 138
90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3980 4012 6666 6698 3980
Size: 32 2654 32 3988
Fragments arranged by size:
3988 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6706
XbaI T'CTAG_A
Cuts at: 3796 4042 3796
Size: 246 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2440 3410 2440
Size: 970 5736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6706
XmaI C'CCGG_G
Cuts at: 1870 4031 1870
Size: 2161 4545
XmnI GAAnn'nnTTC
Cuts at: 4835 4835
Size: 6706
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4514 276
Size: 53 83 186 936 2980 2468
Fragments arranged by size:
2980 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI