Restriction map for
pFN21AA0428
(Circular) (Six-base) MAPSORT of: pFN21AA0428.seq Check: 3732 from: 1 to: 5815
pFN21AA0428 5815 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3878 5262 5530 1632
Size: 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2510 2519 2660 2996 3558 5130 2510
Size: 9 141 336 562 1572 3195
Fragments arranged by size:
3195 1572 562 336 141 9
ApaLI G'TGCA_C
Cuts at: 2975 3872 5028 2975
Size: 897 1156 3762
Fragments arranged by size:
3762 1156 897
ApoI r'AATT_y
Cuts at: 1493 2108 2814 3124 3350 3502 5611 1493
Size: 615 706 310 226 152 2109 1697
Fragments arranged by size:
2109 1697 706 615 310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2531 4369 160
Size: 872 1499 1838 1606
Fragments arranged by size:
1838 1606 1499 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2694 3140 1870
Size: 73 751 446 4545
Fragments arranged by size:
4545 751 446 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2873 3136 4591 618
Size: 325 217 627 543 543 263 1455 1842
Fragments arranged by size:
1842 1455 627 543 543 325 263 217
BanII G_rGCy'C
Cuts at: 1391 3134 1391
Size: 1743 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2103 2137 4006 4040 1903
Size: 34 166 34 1869 34 3678
Fragments arranged by size:
3678 1869 166 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BfrBI ATG'CAT
Cuts at: 2387 2387
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2291 4426 136
Size: 107 122 71 631 605 619 2135 1525
Fragments arranged by size:
2135 1525 631 619 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 5810 2448
Size: 3362 2453
BlpI GC'TnA_GC
Cuts at: 2374 3232 2374
Size: 858 4957
Bme1580I G_kGCm'C
Cuts at: 1790 2335 2979 3876 5032 1790
Size: 545 644 897 1156 2573
Fragments arranged by size:
2573 1156 897 644 545
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2709 2871 2709
Size: 162 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 4475 1239
Size: 934 2302 2579
Fragments arranged by size:
2579 2302 934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2805 3940 4805 5103 2570
Size: 235 1135 865 298 3282
Fragments arranged by size:
3282 1135 865 298 235
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 1186
Size: 1418 4397
BseYI C'CCAG_C
Cuts at: 2295 5018 5514 2295
Size: 2723 496 2596
Fragments arranged by size:
2723 2596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2445 2505 2709 2766 1163
Size: 573 709 60 204 57 4212
Fragments arranged by size:
4212 709 573 204 60 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2979 3876 3961 5032 2979
Size: 897 85 1071 3762
Fragments arranged by size:
3762 1071 897 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2335 2979 3134 3876 3961 5032 1391
Size: 399 545 644 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 545 399 155 85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2509 3170 844
Size: 1665 661 3489
Fragments arranged by size:
3489 1665 661
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2510 2519 2789 794
Size: 1716 9 270 3820
Fragments arranged by size:
3820 1716 270 9
BstBI TT'CG_AA
Cuts at: 2123 4619 2123
Size: 2496 3319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2448 3145 3512 3897 3914 5366 5452 5464
Size: 476 697 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 476 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2808 2970 513
Size: 552 939 804 162 3358
Fragments arranged by size:
3358 939 804 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 2229 3119 2229
Size: 890 4925
DraIII CAC_nnn'GTG
Cuts at: 2664 2664
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2654 4822 809
Size: 507 1338 2168 1802
Fragments arranged by size:
2168 1802 1338 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2283 3262
Size: 54 1425 50 275 18 260 193 979
Cuts at: 3262 4151 5780 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 979 889 275 260 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 3878 4475 5262 5530 1239
Size: 393 541 1705 597 787 268 1524
Fragments arranged by size:
1705 1524 787 597 541 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2417 2417
Size: 5815
EcoRI G'AATT_C
Cuts at: 2814 3124 3502 2814
Size: 310 378 5127
Fragments arranged by size:
5127 378 310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2846 2846
Size: 5815
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 4456 4488 4530
Size: 32 135 32 567 32 2285 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 2285 567 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 3159 3405 669
Size: 988 281 829 392 246 3079
Fragments arranged by size:
3079 988 829 392 281 246
HindIII A'AGCT_T
Cuts at: 748 2371 748
Size: 1623 4192
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 2578 3414 2578
Size: 836 4979
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 4929 5113 1305
Size: 915 2709 184 2007
Fragments arranged by size:
2709 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 10
Size: 54 1425 343 453 3540
Fragments arranged by size:
3540 1425 453 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3099 3773 4132 4291 518
Size: 648 1119 814 674 359 159 2042
Fragments arranged by size:
2042 1119 814 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2507 2516 2780 2804 3231 3908 5056 5301
Size: 901 9 264 24 427 677 1148 245
Cuts at: 5301 1606
Size: 2120
Fragments arranged by size:
2120 1148 901 677 427 264 245 24
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2808 2970 513
Size: 552 939 804 162 3358
Fragments arranged by size:
3358 939 804 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NsiI A_TGCA'T
Cuts at: 2389 2389
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2932 3173 4718 1132
Size: 97 1703 241 1545 2229
Fragments arranged by size:
2229 1703 1545 241 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2417 2417
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2506 2515 2779 2794 3167 830
Size: 1676 9 264 15 373 3478
Fragments arranged by size:
3478 1676 373 264 15 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2507 2516 2780 2804 2507
Size: 9 264 24 5518
Fragments arranged by size:
5518 264 24 9
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SexAI A'CCwGG_T
Cuts at: 2760 2760
Size: 5815
SfcI C'TryA_G
Cuts at: 826 1045 2502 2511 2775 2790 3163 4298 4979
Size: 219 1457 9 264 15 373 1135 681
Cuts at: 4979 5170 826
Size: 191 1471
Fragments arranged by size:
1471 1457 1135 681 373 264 219 191
15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2291 2291
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 2820 3919 4820 5082 820
Size: 197 926 606 271 1099 901 262 1553
Fragments arranged by size:
1553 1099 926 901 606 271 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 2851 1517
Size: 1334 4481
StyI C'CwwG_G
Cuts at: 513 1065 2004 2808 2970 3254 513
Size: 552 939 804 162 284 3074
Fragments arranged by size:
3074 939 804 552 284 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2827 4061 5773
Size: 274 80 33 51 1443 850 1234 1712
Cuts at: 5773 96
Size: 138
Fragments arranged by size:
1712 1443 1234 850 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 3013 3045 5775 5807 2473
Size: 32 508 32 2730 32 2481
Fragments arranged by size:
2730 2481 508 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2141 2141
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI