Restriction map for
pFN21AA0424
(Circular) (Six-base) MAPSORT of: pFN21AA0424.seq Check: 6765 from: 1 to: 6253
pFN21AA0424 6253 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2106 4063 278
Size: 53 83 186 936 570 1957 2468
Fragments arranged by size:
2468 1957 936 570 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 3596 1937
Size: 843 816 4594
Fragments arranged by size:
4594 843 816
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 2459 3511 4316 5700 5968 1632
Size: 782 45 1052 805 1384 268 1917
Fragments arranged by size:
1917 1384 1052 805 782 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 2418 5152 6129 1203
Size: 1215 2734 977 1327
Fragments arranged by size:
2734 1327 1215 977
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AlwNI CAG_nnn'CTG
Cuts at: 3445 3996 5568 3445
Size: 551 1572 4130
Fragments arranged by size:
4130 1572 551
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 6253
ApaLI G'TGCA_C
Cuts at: 4310 5466 4310
Size: 1156 5097
ApoI r'AATT_y
Cuts at: 1493 3312 3562 3788 3940 6049 1493
Size: 1819 250 226 152 2109 1697
Fragments arranged by size:
2109 1819 1697 250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 3578 1870
Size: 73 1635 4545
Fragments arranged by size:
4545 1635 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3200 3233 3200
Size: 33 6220
BamHI G'GATC_C
Cuts at: 3583 3950 3583
Size: 367 5886
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3574 5029 618
Size: 325 217 627 1787 1455 1842
Fragments arranged by size:
1842 1787 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2628 2772 2954 3572 1391
Size: 841 396 144 182 618 4072
Fragments arranged by size:
4072 841 618 396 182 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3013 928
Size: 971 1114 4168
Fragments arranged by size:
4168 1114 971
BbvCI CC'TCA_GC
Cuts at: 2043 2534 2043
Size: 491 5762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4444 4478 1903
Size: 34 2507 34 3678
Fragments arranged by size:
3678 2507 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2760 4145 5355 2760
Size: 1385 1210 3658
Fragments arranged by size:
3658 1385 1210
BclI T'GATC_A
Cuts at: 1548 2016 1548
Size: 468 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4864 136
Size: 107 122 71 631 605 3192 1525
Fragments arranged by size:
3192 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2739 6248 2739
Size: 3509 2744
BlpI GC'TnA_GC
Cuts at: 2766 3670 2766
Size: 904 5349
Bme1580I G_kGCm'C
Cuts at: 1790 2232 4314 5470 1790
Size: 442 2082 1156 2573
Fragments arranged by size:
2573 2082 1156 442
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2932 2979 3984
Size: 416 53 83 399 24 1515 47 1005
Cuts at: 3984 4942 442
Size: 958 1753
Fragments arranged by size:
1753 1515 1005 958 416 399 83 53
47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2522 2712 3254 4913 1239
Size: 804 479 190 542 1659 2579
Fragments arranged by size:
2579 1659 804 542 479 190
Bpu10I CC'TnA_GC
Cuts at: 2043 2534 3254 3363 2043
Size: 491 720 109 4933
Fragments arranged by size:
4933 720 491 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 4378 5243 5541 2354
Size: 2024 865 298 3066
Fragments arranged by size:
3066 2024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2490 4916 882
Size: 617 991 2426 2219
Fragments arranged by size:
2426 2219 991 617
BsaAI yAC'GTr
Cuts at: 493 1204 4044 493
Size: 711 2840 2702
Fragments arranged by size:
2840 2702 711
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 4060 4442 5077
Size: 53 83 186 936 570 1957 382 635
Cuts at: 5077 275
Size: 1451
Fragments arranged by size:
1957 1451 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3069 4686 5084 5358 5505 5916 3069
Size: 1617 398 274 147 411 3406
Fragments arranged by size:
3406 1617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 2495 2525 1178
Size: 30 165 30 753 30 309 30 4906
Fragments arranged by size:
4906 753 309 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2237 3218 1186
Size: 991 60 981 4221
Fragments arranged by size:
4221 991 981 60
BseYI C'CCAG_C
Cuts at: 2231 5456 5952 2231
Size: 3225 496 2532
Fragments arranged by size:
3225 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 2753 1163
Size: 573 525 492 4663
Fragments arranged by size:
4663 573 525 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3703 4464 5492 1540
Size: 275 186 1003 699 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 699 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 2565 2628 2772 2954 4314 4399 5470 2345
Size: 220 63 144 182 1360 85 1071 3128
Fragments arranged by size:
3128 1360 1071 220 182 144 85 63
BsmI GAATG_Cn'
Cuts at: 3178 3764 3857 3178
Size: 586 93 5574
Fragments arranged by size:
5574 586 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2345 2565 2628 2772 2954 3572
Size: 399 442 113 220 63 144 182 618
Cuts at: 3572 4314 4399 5470 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 442 399 220 182
144 113 85 63
BspEI T'CCGG_A
Cuts at: 3069 5916 3069
Size: 2847 3406
BspHI T'CATG_A
Cuts at: 2832 4142 5872 2832
Size: 1310 1730 3213
Fragments arranged by size:
3213 1730 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3463 3522 3608 844
Size: 2619 59 86 3489
Fragments arranged by size:
3489 2619 86 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 4140 1394
Size: 429 1567 750 3507
Fragments arranged by size:
3507 1567 750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 3348 4748 4922 65
Size: 1183 1452 648 1400 174 1396
Fragments arranged by size:
1452 1400 1396 1183 648 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4897 5084 1700
Size: 110 3087 187 2869
Fragments arranged by size:
3087 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 4313 5325 4313
Size: 1012 5241
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6253
BstBI TT'CG_AA
Cuts at: 5057 5057
Size: 6253
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6253
BstXI CCAn_nnnn'nTGG
Cuts at: 3232 3232
Size: 6253
BstYI r'GATC_y
Cuts at: 1972 2739 2985 3583 3950 4335 4352 5804 5890
Size: 767 246 598 367 385 17 1452 86
Cuts at: 5890 5902 6248 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 767 598 385 367 346 246
86 17 12
BstZ17I GTA'TAC
Cuts at: 2781 2781
Size: 6253
BtgI C'CryG_G
Cuts at: 513 1065 2067 3433 513
Size: 552 1002 1366 3333
Fragments arranged by size:
3333 1366 1002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2250 3445 487
Size: 683 56 1024 1195 3295
Fragments arranged by size:
3295 1195 1024 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 3769 4563 4583 2405
Size: 1364 794 20 4075
Fragments arranged by size:
4075 1364 794 20
ClaI AT'CG_AT
Cuts at: 1554 2709 1554
Size: 1155 5098
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 2092 3557 2092
Size: 1465 4788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3158 5260 809
Size: 507 1842 2102 1802
Fragments arranged by size:
2102 1842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2288 3001
Size: 54 1425 50 275 18 240 218 713
Cuts at: 3001 3436 3700 4589 6218 8
Size: 435 264 889 1629 43
Fragments arranged by size:
1629 1425 889 713 435 275 264 240
218 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 3700 1537
Size: 275 1189 699 4090
Fragments arranged by size:
4090 1189 699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 3264 3323 4183 1353
Size: 1049 862 59 860 3423
Fragments arranged by size:
3423 1049 862 860 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 2770 2952 2626
Size: 144 182 5927
Fragments arranged by size:
5927 182 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2414 2459 2522 2712 3254 3511
Size: 393 411 371 45 63 190 542 257
Cuts at: 3511 4316 4913 5700 5968 1239
Size: 805 597 787 268 1524
Fragments arranged by size:
1524 805 787 597 542 411 393 371
268 257 190 63 45
EcoO109I rG'GnC_Cy
Cuts at: 2228 2546 3061 3421 2228
Size: 318 515 360 5060
Fragments arranged by size:
5060 515 360 318
EcoRI G'AATT_C
Cuts at: 3562 3940 3562
Size: 378 5875
EcoRV GAT'ATC
Cuts at: 2467 2467
Size: 6253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2770 3193 2464
Size: 306 423 5524
Fragments arranged by size:
5524 423 306
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 5400 1678
Size: 3722 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2016 2048 2936 2968 4894
Size: 32 135 32 444 32 888 32 1926
Cuts at: 4894 4926 4968 5000 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1926 888 444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3127 3379 3537 3597 3843 669
Size: 988 281 1189 252 158 60 246 3079
Fragments arranged by size:
3079 1189 988 281 252 246 158 60
HindIII A'AGCT_T
Cuts at: 748 3182 748
Size: 2434 3819
HpaI GTT'AAC
Cuts at: 3537 3843 3537
Size: 306 5947
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 5367 5551 1305
Size: 846 3216 184 2007
Fragments arranged by size:
3216 2007 846 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 4191
Fragments arranged by size:
4191 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2568 2610 3104 4211 4570 4729 518
Size: 648 1402 42 494 1107 359 159 2042
Fragments arranged by size:
2042 1402 1107 648 494 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2819 3016 3669 4346 5494 5739 1606
Size: 1213 197 653 677 1148 245 2120
Fragments arranged by size:
2120 1213 1148 677 653 245 197
NcoI C'CATG_G
Cuts at: 513 1065 2067 513
Size: 552 1002 4699
Fragments arranged by size:
4699 1002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2422 3611 5156 1132
Size: 97 1193 1189 1545 2229
Fragments arranged by size:
2229 1545 1193 1189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6253
PciI A'CATG_T
Cuts at: 2418 5152 2418
Size: 2734 3519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3043 3445 1108
Size: 1935 402 3916
Fragments arranged by size:
3916 1935 402
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 3061 3421 2546
Size: 515 360 5378
Fragments arranged by size:
5378 515 360
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 6253
PstI C_TGCA'G
Cuts at: 830 3605 830
Size: 2775 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
PvuII CAG'CTG
Cuts at: 2819 2819
Size: 6253
SacI G_AGCT'C
Cuts at: 2628 2772 2954 2628
Size: 144 182 5927
Fragments arranged by size:
5927 182 144
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SexAI A'CCwGG_T
Cuts at: 3444 3444
Size: 6253
SfcI C'TryA_G
Cuts at: 826 1045 2294 2470 2803 3601 4736 5417 5608
Size: 219 1249 176 333 798 1135 681 191
Cuts at: 5608 826
Size: 1471
Fragments arranged by size:
1471 1249 1135 798 681 333 219 191
176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 4357 5258 5520 820
Size: 197 926 426 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 426 262 197
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 3611 1132
Size: 2479 3774
SspI AAT'ATT
Cuts at: 5 52 2884 4177 5
Size: 47 2832 1293 2081
Fragments arranged by size:
2832 2081 1293 47
StuI AGG'CCT
Cuts at: 1517 2672 1517
Size: 1155 5098
StyI C'CwwG_G
Cuts at: 513 1065 2067 3692 513
Size: 552 1002 1625 3074
Fragments arranged by size:
3074 1625 1002 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 4280 4297 4450 4635 5080 325
Size: 1255 608 2092 17 153 185 445 1498
Fragments arranged by size:
2092 1498 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3084 4499 6211
Size: 274 80 33 51 1443 1107 1415 1712
Cuts at: 6211 96
Size: 138
Fragments arranged by size:
1712 1443 1415 1107 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 6213 6245 2126
Size: 32 4055 32 2134
Fragments arranged by size:
4055 2134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6253
XbaI T'CTAG_A
Cuts at: 3589 3589
Size: 6253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2080
Size: 6253
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 4382 4382
Size: 6253
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2104 4061 276
Size: 53 83 186 936 570 1957 2468
Fragments arranged by size:
2468 1957 936 570 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI