Restriction map for
pFN21AA0411
(Circular) (Six-base) MAPSORT of: pFN21AA0411.seq Check: 2849 from: 1 to: 7930
pFN21AA0411 7930 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5740 278
Size: 53 83 186 936 4204 2468
Fragments arranged by size:
4204 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4748 5273 1937
Size: 2811 525 4594
Fragments arranged by size:
4594 2811 525
Acc65I G'GTAC_C
Cuts at: 5251 5251
Size: 7930
AclI AA'CG_TT
Cuts at: 1563 6057 6430 1563
Size: 4494 373 3063
Fragments arranged by size:
4494 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2486 2567 3538 4946 5993 7377 7645 1632
Size: 854 81 971 1408 1047 1384 268 1917
Fragments arranged by size:
1917 1408 1384 1047 971 854 268 81
AfeI AGC'GCT
Cuts at: 2222 3043 4690 2222
Size: 821 1647 5462
Fragments arranged by size:
5462 1647 821
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7733
AflIII A'CryG_T
Cuts at: 1203 2508 2964 6829 7806 1203
Size: 1305 456 3865 977 1327
Fragments arranged by size:
3865 1327 1305 977 456
AgeI A'CCGG_T
Cuts at: 5130 6761 5130
Size: 1631 6299
AhdI GACnn_n'nnGTC
Cuts at: 3949 4438 4789 6659 3949
Size: 489 351 1870 5220
Fragments arranged by size:
5220 1870 489 351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2624 4862 1184
Size: 1440 2238 4252
Fragments arranged by size:
4252 2238 1440
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2561 2593 2243
Size: 32 286 32 7580
Fragments arranged by size:
7580 286 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3403 3662 5673 7245 3403
Size: 259 2011 1572 4088
Fragments arranged by size:
4088 2011 1572 259
ApaI G_GGCC'C
Cuts at: 4574 4574
Size: 7930
ApaLI G'TGCA_C
Cuts at: 3770 5987 7143 3770
Size: 2217 1156 4557
Fragments arranged by size:
4557 2217 1156
ApoI r'AATT_y
Cuts at: 1493 3329 3595 5239 5465 5617 7726 1493
Size: 1836 266 1644 226 152 2109 1697
Fragments arranged by size:
2109 1836 1697 1644 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6484 160
Size: 872 5452 1606
Fragments arranged by size:
5452 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7930
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2247 4023 4560 4593 4638 4865
Size: 73 243 61 1776 537 33 45 227
Cuts at: 4865 5152 5255 1870
Size: 287 103 4545
Fragments arranged by size:
4545 1776 537 287 243 227 103 73
61 45 33
AvrII C'CTAG_G
Cuts at: 4777 4777
Size: 7930
BamHI G'GATC_C
Cuts at: 3883 5260 5627 3883
Size: 1377 367 6186
Fragments arranged by size:
6186 1377 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3431 4891 5078 5222 5251
Size: 325 217 627 1644 1460 187 144 29
Cuts at: 5251 6706 618
Size: 1455 1842
Fragments arranged by size:
1842 1644 1460 1455 627 325 217 187
144 29
BanII G_rGCy'C
Cuts at: 1391 2187 2463 2484 4574 4658 4923 4959 5249
Size: 796 276 21 2090 84 265 36 290
Cuts at: 5249 1391
Size: 4072
Fragments arranged by size:
4072 2090 796 290 276 265 84 36
21
BbeI G_GCGC'C
Cuts at: 4895 5082 4895
Size: 187 7743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 3621 4824 4835 928
Size: 971 1072 650 1203 11 4023
Fragments arranged by size:
4023 1203 1072 971 650 11
BbvCI CC'TCA_GC
Cuts at: 2597 2597
Size: 7930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4163 4197 4979 5013 6121 6155 1903
Size: 34 2226 34 782 34 1108 34 3678
Fragments arranged by size:
3678 2226 1108 782 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5822 7032 5822
Size: 1210 6720
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7930
BfrBI ATG'CAT
Cuts at: 4056 4056
Size: 7930
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2834 5072 5076
Size: 107 122 71 631 605 1162 2238 4
Cuts at: 5076 5113 6541 136
Size: 37 1428 1525
Fragments arranged by size:
2238 1525 1428 1162 631 605 122 107
71 37 4
BglII A'GATC_T
Cuts at: 7925 7925
Size: 7930
BlpI GC'TnA_GC
Cuts at: 2217 3045 5206 5347 2217
Size: 828 2161 141 4800
Fragments arranged by size:
4800 2161 828 141
Bme1580I G_kGCm'C
Cuts at: 1790 3774 4574 4737 5225 5991 7147 1790
Size: 1984 800 163 488 766 1156 2573
Fragments arranged by size:
2573 1984 1156 800 766 488 163
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7930
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3853 5661 6619
Size: 416 53 83 399 24 2436 1808 958
Cuts at: 6619 442
Size: 1753
Fragments arranged by size:
2436 1808 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7930
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 4474 4860 2242
Size: 2232 386 5312
Fragments arranged by size:
5312 2232 386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2135 2225 2897 3521 3770 4083 4421
Size: 890 6 90 672 624 249 313 338
Cuts at: 4421 4817 4937 6590 1239
Size: 396 120 1653 2579
Fragments arranged by size:
2579 1653 890 672 624 396 338 313
249 120 90 6
Bpu10I CC'TnA_GC
Cuts at: 2597 4364 2597
Size: 1767 6163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3095 3377 6055 6920 7218 2978
Size: 117 282 2678 865 298 3690
Fragments arranged by size:
3690 2678 865 298 282 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2331 2586 2873 3248 6593 882
Size: 617 832 255 287 375 3345 2219
Fragments arranged by size:
3345 2219 832 617 375 287 255
BsaAI yAC'GTr
Cuts at: 493 1204 5721 493
Size: 711 4517 2702
Fragments arranged by size:
4517 2702 711
BsaBI GATnn'nnATC
Cuts at: 7924 7924
Size: 7930
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4528 4892 5079 5177
Size: 53 83 186 936 2995 364 187 98
Cuts at: 5177 5737 6119 6754 275
Size: 560 382 635 1451
Fragments arranged by size:
2995 1451 936 635 560 382 364 187
186 98 83 53
BsaWI w'CCGG_w
Cuts at: 2847 5130 6363 6761 7035 7182 7593 2847
Size: 2283 1233 398 274 147 411 3184
Fragments arranged by size:
3184 2283 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 2561 2591 3839
Size: 30 165 30 840 30 288 30 1248
Cuts at: 3839 3869 4259 4289 1178
Size: 30 390 30 4819
Fragments arranged by size:
4819 1248 840 390 288 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 2270 2471 2582 3140 3301 5014 1186
Size: 1032 52 201 111 558 161 1713 4102
Fragments arranged by size:
4102 1713 1032 558 201 161 111 52
BseYI C'CCAG_C
Cuts at: 2631 3757 4016 4574 4610 5082 7133 7629 2631
Size: 1126 259 558 36 472 2051 496 2932
Fragments arranged by size:
2932 2051 1126 558 496 472 259 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4832 1163
Size: 573 3096 4261
Fragments arranged by size:
4261 3096 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3224 5117 5172 5380 6141 7169
Size: 275 186 1223 1893 55 208 761 1028
Cuts at: 7169 1540
Size: 2301
Fragments arranged by size:
2301 1893 1223 1028 761 275 208 186
55
BsiHKAI G_wGCw'C
Cuts at: 2187 2256 2463 2484 3774 4455 4841 5991 6076
Size: 69 207 21 1290 681 386 1150 85
Cuts at: 6076 7147 2187
Size: 1071 2970
Fragments arranged by size:
2970 1290 1150 1071 681 386 207 85
69 21
BsmI GAATG_Cn'
Cuts at: 2920 3431 5441 5534 2920
Size: 511 2010 93 5316
Fragments arranged by size:
5316 2010 511 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2256 2463 2484 3774 4455 4574
Size: 399 397 69 207 21 1290 681 119
Cuts at: 4574 4658 4737 4841 4923 4959 5225 5249 5991
Size: 84 79 104 82 36 266 24 742
Cuts at: 5991 6076 7147 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1290 1071 742 681 399 397 266
207 119 104 85 84 82 79 69
36 24 21
BspEI T'CCGG_A
Cuts at: 2847 7593 2847
Size: 4746 3184
BspHI T'CATG_A
Cuts at: 2364 2493 3822 4000 5819 7549 2364
Size: 129 1329 178 1819 1730 2745
Fragments arranged by size:
2745 1819 1730 1329 178 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2616 4760 5285 844
Size: 1772 2144 525 3489
Fragments arranged by size:
3489 2144 1772 525
BsrBI CCG'CTC
Cuts at: 1394 1823 5817 1394
Size: 429 3994 3507
Fragments arranged by size:
3994 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2043 3276 4400 6425 6599 65
Size: 1183 795 1233 1124 2025 174 1396
Fragments arranged by size:
2025 1396 1233 1183 1124 795 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 4284 5116 5130 6574 6761 1700
Size: 110 815 1659 832 14 1444 187 2869
Fragments arranged by size:
2869 1659 1444 832 815 187 110 14
BsrGI T'GTAC_A
Cuts at: 96 4330 96
Size: 4234 3696
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7930
BssSI C'ACGA_G
Cuts at: 2453 2618 4850 5990 7002 2453
Size: 165 2232 1140 1012 3381
Fragments arranged by size:
3381 2232 1140 1012 165
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4855 794
Size: 4061 3869
BstBI TT'CG_AA
Cuts at: 6734 6734
Size: 7930
BstEII G'GTnAC_C
Cuts at: 1184 2470 1184
Size: 1286 6644
BstXI CCAn_nnnn'nTGG
Cuts at: 4130 4130
Size: 7930
BstYI r'GATC_y
Cuts at: 1972 2073 3549 3566 3777 3883 5260 5627 6012
Size: 101 1476 17 211 106 1377 367 385
Cuts at: 6012 6029 7481 7567 7579 7925 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1476 1452 1377 385 367 346 211
106 101 86 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 3711 3711
Size: 7930
BtgI C'CryG_G
Cuts at: 513 1065 4446 5226 513
Size: 552 3381 780 3217
Fragments arranged by size:
3381 3217 780 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4167 487
Size: 683 56 2941 4250
Fragments arranged by size:
4250 2941 683 56
BtsI GCAGTG_nn'
Cuts at: 2925 4399 5446 6240 6260 2925
Size: 1474 1047 794 20 4595
Fragments arranged by size:
4595 1474 1047 794 20
ClaI AT'CG_AT
Cuts at: 1554 2802 4110 1554
Size: 1248 1308 5374
Fragments arranged by size:
5374 1308 1248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7895
DraI TTT'AAA
Cuts at: 3334 3672 5234 3334
Size: 338 1562 6030
Fragments arranged by size:
6030 1562 338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3194 6937 809
Size: 507 1878 3743 1802
Fragments arranged by size:
3743 1878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3102 3897 5114
Size: 54 1425 50 275 18 1272 795 1217
Cuts at: 5114 5169 5377 6266 7895 8
Size: 55 208 889 1629 43
Fragments arranged by size:
1629 1425 1272 1217 889 795 275 208
55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5114 5169 5377 1537
Size: 275 3302 55 208 4090
Fragments arranged by size:
4090 3302 275 208 55
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2669 3839 4426 5860 1353
Size: 1070 246 1170 587 1434 3423
Fragments arranged by size:
3423 1434 1170 1070 587 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 2372 5053 5078 6517 6891
Size: 1064 138 564 219 2681 25 1439 374
Cuts at: 6891 7037 387
Size: 146 1280
Fragments arranged by size:
2681 1439 1280 1064 564 374 219 146
138 25
EcoICRI GAG'CTC
Cuts at: 2185 2461 2482 2185
Size: 276 21 7633
Fragments arranged by size:
7633 276 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2135 2225 2486 2567 2897 3521
Size: 393 497 6 90 261 81 330 624
Cuts at: 3521 3538 3770 4083 4421 4817 4937 4946 5993
Size: 17 232 313 338 396 120 9 1047
Cuts at: 5993 6590 7377 7645 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1047 787 624 597 497 396 393
338 330 313 268 261 232 120 90
81 17 9 6
EcoO109I rG'GnC_Cy
Cuts at: 3943 4037 4236 4564 4571 4807 4940 3943
Size: 94 199 328 7 236 133 6933
Fragments arranged by size:
6933 328 236 199 133 94 7
EcoRI G'AATT_C
Cuts at: 5239 5617 5239
Size: 378 7552
EcoRV GAT'ATC
Cuts at: 3649 3735 3649
Size: 86 7844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3638 3638
Size: 7930
FspI TGC'GCA
Cuts at: 6436 6436
Size: 7930
HaeII r_GCGC'y
Cuts at: 1678 2224 2638 3045 4692 4724 4895 5082 7077
Size: 546 414 407 1647 32 171 187 1995
Cuts at: 7077 1678
Size: 2531
Fragments arranged by size:
2531 1995 1647 546 414 407 187 171
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 3719 3751 3935
Size: 32 135 32 530 32 1585 32 184
Cuts at: 3935 3967 4337 4369 4403 4424 4435 4456 4503
Size: 32 370 32 34 21 11 21 47
Cuts at: 4503 4535 4775 4807 6571 6603 6645 6677 1373
Size: 32 240 32 1764 32 42 32 2626
Fragments arranged by size:
2626 1764 1585 530 370 240 184 135
47 42 34 32 32 32 32 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4120 4749 5274 5520 669
Size: 988 281 2182 629 525 246 3079
Fragments arranged by size:
3079 2182 988 629 525 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7930
HpaI GTT'AAC
Cuts at: 5520 5520
Size: 7930
KasI G'GCGC_C
Cuts at: 4891 5078 4891
Size: 187 7743
KpnI G_GTAC'C
Cuts at: 5255 5255
Size: 7930
MfeI C'AATT_G
Cuts at: 5529 5529
Size: 7930
MluI A'CGCG_T
Cuts at: 7806 7806
Size: 7930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3235 3805 4380 7044 7228 1305
Size: 1930 570 575 2664 184 2007
Fragments arranged by size:
2664 2007 1930 575 570 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 3899 10
Size: 54 1425 343 1272 795 4041
Fragments arranged by size:
4041 1425 1272 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2492 2957 2963 3969 4128 5033
Size: 648 1197 129 465 6 1006 159 905
Cuts at: 5033 5888 6247 6406 518
Size: 855 359 159 2042
Fragments arranged by size:
2042 1197 1006 905 855 648 465 359
159 159 129 6
MspA1I CmG'CkG
Cuts at: 1606 2086 2137 2864 3088 5210 5346 6023 7171
Size: 480 51 727 224 2122 136 677 1148
Cuts at: 7171 7416 1606
Size: 245 2120
Fragments arranged by size:
2122 2120 1148 727 677 480 245 224
136 51
NaeI GCC'GGC
Cuts at: 4286 4286
Size: 7930
NarI GG'CG_CC
Cuts at: 4892 5079 4892
Size: 187 7743
NcoI C'CATG_G
Cuts at: 513 1065 5226 513
Size: 552 4161 3217
Fragments arranged by size:
4161 3217 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7930
NgoMIV G'CCGG_C
Cuts at: 4284 4284
Size: 7930
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7930
NotI GC'GGCC_GC
Cuts at: 5377 5377
Size: 7930
NruI TCG'CGA
Cuts at: 2128 2128
Size: 7930
NsiI A_TGCA'T
Cuts at: 4058 4058
Size: 7930
NspI r_CATG'y
Cuts at: 1132 1229 2512 2968 3175 5288 6833 1132
Size: 97 1283 456 207 2113 1545 2229
Fragments arranged by size:
2229 2113 1545 1283 456 207 97
PasI CC'CwG_GG
Cuts at: 1348 3403 1348
Size: 2055 5875
PciI A'CATG_T
Cuts at: 2508 2964 6829 2508
Size: 456 3865 3609
Fragments arranged by size:
3865 3609 456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7930
PfoI T'CCnGG_A
Cuts at: 2151 2151
Size: 7930
PmeI GTTT'AAAC
Cuts at: 5234 5234
Size: 7930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 2561 2593 5177 5209 5999 6031 7512
Size: 32 286 32 2584 32 790 32 1481
Cuts at: 7512 7544 2243
Size: 32 2629
Fragments arranged by size:
2629 2584 1481 790 286 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3943 4037 3943
Size: 94 7836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7930
PsiI TTA'TAA
Cuts at: 5500 5500
Size: 7930
PspOMI G'GGCC_C
Cuts at: 4570 4570
Size: 7930
PspXI vC'TCGA_Gb
Cuts at: 2186 2186
Size: 7930
PstI C_TGCA'G
Cuts at: 830 2136 2448 3093 3375 5282 830
Size: 1306 312 645 282 1907 3478
Fragments arranged by size:
3478 1907 1306 645 312 282
PvuI CG_AT'CG
Cuts at: 2001 3224 2001
Size: 1223 6707
PvuII CAG'CTG
Cuts at: 2086 2137 2864 3088 2086
Size: 51 727 224 6928
Fragments arranged by size:
6928 727 224 51
SacI G_AGCT'C
Cuts at: 2187 2463 2484 2187
Size: 276 21 7633
Fragments arranged by size:
7633 276 21
SalI G'TCGA_C
Cuts at: 1936 4747 5272 1936
Size: 2811 525 4594
Fragments arranged by size:
4594 2811 525
SbfI CC_TGCA'GG
Cuts at: 5282 5282
Size: 7930
ScaI AGT'ACT
Cuts at: 2192 2690 6178 7890 2192
Size: 498 3488 1712 2232
Fragments arranged by size:
3488 2232 1712 498
SfcI C'TryA_G
Cuts at: 826 1045 2132 2444 3089 3371 5278 6413 7094
Size: 219 1087 312 645 282 1907 1135 681
Cuts at: 7094 7285 826
Size: 191 1471
Fragments arranged by size:
1907 1471 1135 1087 681 645 312 282
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5113 5113
Size: 7930
SfoI GGC'GCC
Cuts at: 4893 5080 4893
Size: 187 7743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7930
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7930
SmaI CCC'GGG
Cuts at: 1872 2249 4025 4562 4595 4640 5257 1872
Size: 377 1776 537 33 45 617 4545
Fragments arranged by size:
4545 1776 617 537 377 45 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 2993 3110 3392 4865 6034
Size: 197 926 243 807 117 282 1473 1169
Cuts at: 6034 6935 7197 820
Size: 901 262 1553
Fragments arranged by size:
1553 1473 1169 926 901 807 282 262
243 197 117
SnaBI TAC'GTA
Cuts at: 493 5721 493
Size: 5228 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7930
SphI G_CATG'C
Cuts at: 1132 5288 1132
Size: 4156 3774
SspI AAT'ATT
Cuts at: 5 52 4076 5854 5
Size: 47 4024 1778 2081
Fragments arranged by size:
4024 2081 1778 47
StuI AGG'CCT
Cuts at: 1517 4418 1517
Size: 2901 5029
StyI C'CwwG_G
Cuts at: 513 1065 4777 5226 5369 513
Size: 552 3712 449 143 3074
Fragments arranged by size:
3712 3074 552 449 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5957 5974 6127 6312 6757 325
Size: 1255 4377 17 153 185 445 1498
Fragments arranged by size:
4377 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2688 4320
Size: 274 80 33 51 1443 213 498 1632
Cuts at: 4320 4330 6176 7888 96
Size: 10 1846 1712 138
Fragments arranged by size:
1846 1712 1632 1443 498 274 213 138
80 51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3558 3590 4259 4291 7890 7922 3558
Size: 32 669 32 3599 32 3566
Fragments arranged by size:
3599 3566 669 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3229 1316
Size: 1913 6017
XbaI T'CTAG_A
Cuts at: 5266 5266
Size: 7930
XhoI C'TCGA_G
Cuts at: 1943 2186 4865 1943
Size: 243 2679 5008
Fragments arranged by size:
5008 2679 243
XmaI C'CCGG_G
Cuts at: 1870 2247 4023 4560 4593 4638 5255 1870
Size: 377 1776 537 33 45 617 4545
Fragments arranged by size:
4545 1776 617 537 377 45 33
XmnI GAAnn'nnTTC
Cuts at: 2576 3720 5192 6059 2576
Size: 1144 1472 867 4447
Fragments arranged by size:
4447 1472 1144 867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5738 276
Size: 53 83 186 936 4204 2468
Fragments arranged by size:
4204 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BsiWI BsmBI BstZ17I DraIII EcoNI
FseI FspAI PacI PmlI PsrI RsrII SacII SanDI
SapI SexAI SrfI SwaI XcmI