Restriction map for
pFN21AA0409
(Circular) (Six-base) MAPSORT of: pFN21AA0409.seq Check: 4864 from: 1 to: 6073
pFN21AA0409 6073 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3416 1937
Size: 1479 4594
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2406 3170 3332 4136 5520 5788 1632
Size: 651 123 764 162 804 1384 268 1917
Fragments arranged by size:
1917 1384 804 764 651 268 162 123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 2446 2740 3816 5388 2446
Size: 294 1076 1572 3131
Fragments arranged by size:
3131 1572 1076 294
ApaI G_GGCC'C
Cuts at: 2061 2143 2306 2752 2790 3306 3321 2061
Size: 82 163 446 38 516 15 4813
Fragments arranged by size:
4813 516 446 163 82 38 15
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2431 3382 3608 3760 5869 1493
Size: 938 951 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 938 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2703 3398 1870
Size: 73 760 695 4545
Fragments arranged by size:
4545 760 695 73
AvrII C'CTAG_G
Cuts at: 3023 3023
Size: 6073
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2623 3394 4849 618
Size: 325 217 627 836 771 1455 1842
Fragments arranged by size:
1842 1455 836 771 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2108 2143 2306 2725 2752 2790 3306
Size: 670 47 35 163 419 27 38 516
Cuts at: 3306 3321 3392 1391
Size: 15 71 4072
Fragments arranged by size:
4072 670 516 419 163 71 47 38
35 27 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2666 2826 928
Size: 971 767 160 4175
Fragments arranged by size:
4175 971 767 160
BbvCI CC'TCA_GC
Cuts at: 2643 3177 2643
Size: 534 5539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2990 3965 5175 2990
Size: 975 1210 3888
Fragments arranged by size:
3888 1210 975
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2147 2729
Size: 107 122 71 631 605 361 114 582
Cuts at: 2729 4684 136
Size: 1955 1525
Fragments arranged by size:
1955 1525 631 605 582 361 122 114
107 71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3327 3490 3327
Size: 163 5910
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2143 2306 2752 2790 3306 3321 4134
Size: 271 82 163 446 38 516 15 813
Cuts at: 4134 5290 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 813 516 446 271 163 82
38 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2033 3309 3804
Size: 416 53 83 399 24 616 1276 495
Cuts at: 3804 4762 442
Size: 958 1753
Fragments arranged by size:
1753 1276 958 616 495 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3080 4733 1239
Size: 1841 1653 2579
Fragments arranged by size:
2579 1841 1653
Bpu10I CC'TnA_GC
Cuts at: 2643 3177 2643
Size: 534 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3167 4736 882
Size: 617 1668 1569 2219
Fragments arranged by size:
2219 1668 1569 617
BsaAI yAC'GTr
Cuts at: 493 1204 3864 493
Size: 711 2660 2702
Fragments arranged by size:
2702 2660 711
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2982 4506 4904 5178 5325 5736 2982
Size: 1524 398 274 147 411 3319
Fragments arranged by size:
3319 1524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 3053 3083 1178
Size: 30 165 30 687 30 933 30 4168
Fragments arranged by size:
4168 933 687 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2213 2216 2219 2222 3146 1186
Size: 894 133 3 3 3 924 4113
Fragments arranged by size:
4113 924 894 133 3 3 3
BseYI C'CCAG_C
Cuts at: 2163 2174 2439 2638 2789 5276 5772 2163
Size: 11 265 199 151 2487 496 2464
Fragments arranged by size:
2487 2464 496 265 199 151 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2784 1163
Size: 573 1048 4452
Fragments arranged by size:
4452 1048 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4134 4219 5290 4134
Size: 85 1071 4917
Fragments arranged by size:
4917 1071 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
BsmBI CGTCTCn'nnnn_
Cuts at: 2813 2813
Size: 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2108 2143 2306 2725 2752 2790
Size: 399 271 47 35 163 419 27 38
Cuts at: 2790 3306 3321 3392 4134 4219 5290 1391
Size: 516 15 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 419 399 271 163
85 71 47 38 35 27 15
BspEI T'CCGG_A
Cuts at: 2982 5736 2982
Size: 2754 3319
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2995 4568 4742 65
Size: 1183 1747 1573 174 1396
Fragments arranged by size:
1747 1573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 4717 4904 1700
Size: 110 764 2143 187 2869
Fragments arranged by size:
2869 2143 764 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 794
Size: 1452 4621
BstBI TT'CG_AA
Cuts at: 2010 3205 4877 2010
Size: 1195 1672 3206
Fragments arranged by size:
3206 1672 1195
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 3299 3299
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 2908 3403 3770 4155 4172 5624 5710 5722
Size: 936 495 367 385 17 1452 86 12
Cuts at: 5722 6068 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 936 495 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2619 2826 2619
Size: 207 5866
BtgI C'CryG_G
Cuts at: 513 1065 2046 2848 513
Size: 552 981 802 3738
Fragments arranged by size:
3738 981 802 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2884 487
Size: 683 56 1658 3676
Fragments arranged by size:
3676 1658 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2380 2570 2800 2879 3589 4383 4403 2320
Size: 60 190 230 79 710 794 20 3990
Fragments arranged by size:
3990 794 710 230 190 79 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DraIII CAC_nnn'GTG
Cuts at: 3036 3036
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2668 3026 5080 809
Size: 507 1352 358 2054 1802
Fragments arranged by size:
2054 1802 1352 507 358
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2876 3520
Size: 54 1425 50 275 18 297 749 644
Cuts at: 3520 4409 6038 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 749 644 297 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2816 4003 1353
Size: 654 809 1187 3423
Fragments arranged by size:
3423 1187 809 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2067 2584 4660 5034 5180 387
Size: 1064 138 478 517 2076 374 146 1280
Fragments arranged by size:
2076 1280 1064 517 478 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2406 3080 3170 3332 4136 4733
Size: 393 651 123 674 90 162 804 597
Cuts at: 4733 5520 5788 1239
Size: 787 268 1524
Fragments arranged by size:
1524 804 787 674 651 597 393 268
162 123 90
EcoNI CCTnn'n_nnAGG
Cuts at: 3131 3312 3131
Size: 181 5892
EcoO109I rG'GnC_Cy
Cuts at: 2140 2302 2468 2635 2647 2748 2786 3256 3303
Size: 162 166 167 12 101 38 470 47
Cuts at: 3303 3317 2140
Size: 14 4896
Fragments arranged by size:
4896 470 167 166 162 101 47 38
14 12
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3253 3253
Size: 6073
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 2116 5220 1678
Size: 438 3104 2531
Fragments arranged by size:
3104 2531 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 3053 3085 4714
Size: 32 135 32 566 32 883 32 1629
Cuts at: 4714 4746 4788 4820 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1629 883 566 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 3417 3663 669
Size: 988 281 520 959 246 3079
Fragments arranged by size:
3079 988 959 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5187 5371 1305
Size: 3882 184 2007
Fragments arranged by size:
3882 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 2878 10
Size: 54 1425 343 297 749 3205
Fragments arranged by size:
3205 1425 749 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4031 4390 4549 518
Size: 648 2865 359 159 2042
Fragments arranged by size:
2865 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2642 2737 2850 2920 3489 4166 5314
Size: 442 594 95 113 70 569 677 1148
Cuts at: 5314 5559 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 594 569 442 245 113
95 70
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5521
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 2423 3431 4976 1132
Size: 97 1194 1008 1545 2229
Fragments arranged by size:
2229 1545 1194 1008 97
PasI CC'CwG_GG
Cuts at: 1348 2519 1348
Size: 1171 4902
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2125 2446 2478 1108
Size: 1017 321 32 4703
Fragments arranged by size:
4703 1017 321 32
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2468 3256 2468
Size: 788 5285
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PspOMI G'GGCC_C
Cuts at: 2057 2139 2302 2748 2786 3302 3317 2057
Size: 82 163 446 38 516 15 4813
Fragments arranged by size:
4813 516 446 163 82 38 15
PstI C_TGCA'G
Cuts at: 830 2886 3345 3425 830
Size: 2056 459 80 3478
Fragments arranged by size:
3478 2056 459 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2642 2642
Size: 6073
SacII CC_GC'GG
Cuts at: 2049 2851 2049
Size: 802 5271
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SapI GCTCTTCn'nnn_
Cuts at: 2007 2007
Size: 6073
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 2491 3147 4321 6033 2491
Size: 656 1174 1712 2531
Fragments arranged by size:
2531 1712 1174 656
SfcI C'TryA_G
Cuts at: 826 1045 2882 3341 3421 4556 5237 5428 826
Size: 219 1837 459 80 1135 681 191 1471
Fragments arranged by size:
1837 1471 1135 681 459 219 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2033 2033
Size: 6073
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4177 5078 5340 820
Size: 197 926 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 3339 1517
Size: 1822 4251
StyI C'CwwG_G
Cuts at: 513 1065 2053 3023 3252 3321 3512 513
Size: 552 988 970 229 69 191 3074
Fragments arranged by size:
3074 988 970 552 229 191 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4100 4117 4270 4455 4900 325
Size: 1255 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2489 2778 3145
Size: 274 80 33 51 1443 512 289 367
Cuts at: 3145 4319 6031 96
Size: 1174 1712 138
Fragments arranged by size:
1712 1443 1174 512 367 289 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 4202 4202
Size: 6073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI