Restriction map for
pFN21AA0408
(Circular) (Six-base) MAPSORT of: pFN21AA0408.seq Check: 2232 from: 1 to: 7546
pFN21AA0408 7546 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3957 3957
Size: 7546
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5356 278
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4889 1937
Size: 2952 4594
Acc65I G'GTAC_C
Cuts at: 4867 4867
Size: 7546
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2397 3599 3869 4139 5609 6993 7261 1632
Size: 765 1202 270 270 1470 1384 268 1917
Fragments arranged by size:
1917 1470 1384 1202 765 270 270 268
AfeI AGC'GCT
Cuts at: 4208 4208
Size: 7546
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7349
AflIII A'CryG_T
Cuts at: 1203 6445 7422 1203
Size: 5242 977 1327
Fragments arranged by size:
5242 1327 977
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 6275 6275
Size: 7546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3536 4178 4673 1184
Size: 2352 642 495 4057
Fragments arranged by size:
4057 2352 642 495
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3657 3689 3657
Size: 32 7514
AlwNI CAG_nnn'CTG
Cuts at: 2072 2227 2589 3949 5289 6861 2072
Size: 155 362 1360 1340 1572 2757
Fragments arranged by size:
2757 1572 1360 1340 362 155
ApaI G_GGCC'C
Cuts at: 2957 4332 4416 2957
Size: 1375 84 6087
Fragments arranged by size:
6087 1375 84
ApaLI G'TGCA_C
Cuts at: 5603 6759 5603
Size: 1156 6390
ApoI r'AATT_y
Cuts at: 1493 4855 5081 5233 7342 1493
Size: 3362 226 152 2109 1697
Fragments arranged by size:
3362 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4127 4127
Size: 7546
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 2432 2445 2474 4409 4679 4871 1870
Size: 73 489 13 29 1935 270 192 4545
Fragments arranged by size:
4545 1935 489 270 192 73 29 13
BamHI G'GATC_C
Cuts at: 3225 4876 5243 3225
Size: 1651 367 5528
Fragments arranged by size:
5528 1651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 4077 4405 4684 4709
Size: 325 217 627 542 1748 328 279 25
Cuts at: 4709 4867 6322 618
Size: 158 1455 1842
Fragments arranged by size:
1842 1748 1455 627 542 328 325 279
217 158 25
BanII G_rGCy'C
Cuts at: 1391 2122 2781 2957 3675 3765 4035 4149 4332
Size: 731 659 176 718 90 270 114 183
Cuts at: 4332 4416 4722 4865 1391
Size: 84 306 143 4072
Fragments arranged by size:
4072 731 718 659 306 270 183 176
143 114 90 84
BbeI G_GCGC'C
Cuts at: 2333 4409 4713 2333
Size: 2076 304 5166
Fragments arranged by size:
5166 2076 304
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3969 4810 928
Size: 971 2070 841 3664
Fragments arranged by size:
3664 2070 971 841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3149 3183 3799 3833 4471 4505 5737
Size: 34 1212 34 616 34 638 34 1232
Cuts at: 5737 5771 1903
Size: 34 3678
Fragments arranged by size:
3678 1232 1212 638 616 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5438 6648 5438
Size: 1210 6336
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2315 6157
Size: 107 122 71 631 605 403 240 3842
Cuts at: 6157 136
Size: 1525
Fragments arranged by size:
3842 1525 631 605 403 240 122 107
71
BglII A'GATC_T
Cuts at: 3574 7541 3574
Size: 3967 3579
BlpI GC'TnA_GC
Cuts at: 2613 4148 4963 2613
Size: 1535 815 5196
Fragments arranged by size:
5196 1535 815
Bme1580I G_kGCm'C
Cuts at: 1790 2466 2957 4332 4416 4689 5607 6763 1790
Size: 676 491 1375 84 273 918 1156 2573
Fragments arranged by size:
2573 1375 1156 918 676 491 273 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 5277 6235
Size: 416 53 83 399 24 760 3100 958
Cuts at: 6235 442
Size: 1753
Fragments arranged by size:
3100 1753 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7546
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 4366 2662
Size: 1704 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3335 3659 3848 4022 6206 1239
Size: 2096 324 189 174 2184 2579
Fragments arranged by size:
2579 2184 2096 324 189 174
Bpu10I CC'TnA_GC
Cuts at: 3776 3776
Size: 7546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 3086 3311 3749 5671 6536 6834 2780
Size: 306 225 438 1922 865 298 3492
Fragments arranged by size:
3492 1922 865 438 306 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 6209 882
Size: 617 1401 3309 2219
Fragments arranged by size:
3309 2219 1401 617
BsaAI yAC'GTr
Cuts at: 493 1204 4543 5337 493
Size: 711 3339 794 2702
Fragments arranged by size:
3339 2702 794 711
BsaBI GATnn'nnATC
Cuts at: 7540 7540
Size: 7546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2321 2330 4260 4406
Size: 53 83 186 936 788 9 1930 146
Cuts at: 4406 4710 5353 5735 6370 275
Size: 304 643 382 635 1451
Fragments arranged by size:
1930 1451 936 788 643 635 382 304
186 146 83 53 9
BsaWI w'CCGG_w
Cuts at: 2705 5979 6377 6651 6798 7209 2705
Size: 3274 398 274 147 411 3042
Fragments arranged by size:
3274 3042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 3657 3687 1178
Size: 30 165 30 1038 30 1186 30 5037
Fragments arranged by size:
5037 1186 1038 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2307 2450 2520 2538 2541 2653 3080 3176
Size: 1121 143 70 18 3 112 427 96
Cuts at: 3176 3575 4019 4127 4370 4592 4730 1186
Size: 399 444 108 243 222 138 4002
Fragments arranged by size:
4002 1121 444 427 399 243 222 143
138 112 108 96 70 18 3
BseYI C'CCAG_C
Cuts at: 2958 3743 6749 7245 2958
Size: 785 3006 496 3259
Fragments arranged by size:
3259 3006 785 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 6640
Fragments arranged by size:
6640 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2826 4438 4495 4675 4705 4996
Size: 275 186 825 1612 57 180 30 291
Cuts at: 4996 5757 6785 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1612 1028 825 761 291 275 186
180 57 30
BsiHKAI G_wGCw'C
Cuts at: 2122 2781 3675 3765 4035 4149 4517 4608 5607
Size: 659 894 90 270 114 368 91 999
Cuts at: 5607 5692 6763 2122
Size: 85 1071 2905
Fragments arranged by size:
2905 1071 999 894 659 368 270 114
91 90 85
BsiWI C'GTAC_G
Cuts at: 2605 2605
Size: 7546
BsmI GAATG_Cn'
Cuts at: 5057 5150 5057
Size: 93 7453
BsmBI CGTCTCn'nnnn_
Cuts at: 3962 3962
Size: 7546
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2466 2781 2957 3675 3765 4035
Size: 399 332 344 315 176 718 90 270
Cuts at: 4035 4149 4332 4416 4517 4608 4689 4722 4865
Size: 114 183 84 101 91 81 33 143
Cuts at: 4865 5607 5692 6763 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 718 399 344 332 315
270 183 176 143 114 101 91 90
85 84 81 33
BspEI T'CCGG_A
Cuts at: 2705 7209 2705
Size: 4504 3042
BspHI T'CATG_A
Cuts at: 3906 5435 7165 3906
Size: 1529 1730 4287
Fragments arranged by size:
4287 1730 1529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3957 4901 844
Size: 3113 944 3489
Fragments arranged by size:
3489 3113 944
BsrBI CCG'CTC
Cuts at: 1394 1823 2345 2491 2806 2985 3904 4461 5433
Size: 429 522 146 315 179 919 557 972
Cuts at: 5433 1394
Size: 3507
Fragments arranged by size:
3507 972 919 557 522 429 315 179
146
BsrDI GCAATG_nn'
Cuts at: 65 1248 6041 6215 65
Size: 1183 4793 174 1396
Fragments arranged by size:
4793 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2531 2690 4494 6190 6377 1700
Size: 110 382 339 159 1804 1696 187 2869
Fragments arranged by size:
2869 1804 1696 382 339 187 159 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7546
BssHII G'CGCG_C
Cuts at: 1925 4071 4127 4532 1925
Size: 2146 56 405 4939
Fragments arranged by size:
4939 2146 405 56
BssSI C'ACGA_G
Cuts at: 3194 3668 4510 4607 5606 6618 3194
Size: 474 842 97 999 1012 4122
Fragments arranged by size:
4122 1012 999 842 474 97
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 3187 3949 4612 794
Size: 1433 960 762 663 3728
Fragments arranged by size:
3728 1433 960 762 663
BstBI TT'CG_AA
Cuts at: 6350 6350
Size: 7546
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7546
BstXI CCAn_nnnn'nTGG
Cuts at: 3805 3805
Size: 7546
BstYI r'GATC_y
Cuts at: 1972 3225 3574 3686 3700 4876 5243 5628 5645
Size: 1253 349 112 14 1176 367 385 17
Cuts at: 5645 7097 7183 7195 7541 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1253 1176 385 367 349 346
112 86 17 14 12
Bsu36I CC'TnA_GG
Cuts at: 3869 3869
Size: 7546
BtgI C'CryG_G
Cuts at: 513 1065 2826 4237 4705 513
Size: 552 1761 1411 468 3354
Fragments arranged by size:
3354 1761 1411 552 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3891 4486 487
Size: 683 56 2665 595 3547
Fragments arranged by size:
3547 2665 683 595 56
BtsI GCAGTG_nn'
Cuts at: 5062 5856 5876 5062
Size: 794 20 6732
Fragments arranged by size:
6732 794 20
ClaI AT'CG_AT
Cuts at: 1554 3114 1554
Size: 1560 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7511
DraI TTT'AAA
Cuts at: 4810 4850 4810
Size: 40 7506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2400 2823
Size: 54 1425 50 275 18 339 231 423
Cuts at: 2823 2913 3633 3894 3993 4060 4545 4993 5882
Size: 90 720 261 99 67 485 448 889
Cuts at: 5882 7511 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 720 485 448 423 339
275 261 231 99 90 67 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2823 4993 1537
Size: 275 1011 2170 4090
Fragments arranged by size:
4090 2170 1011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2671 3499 5476 1353
Size: 1318 828 1977 3423
Fragments arranged by size:
3423 1977 1318 828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 2914 2963 3869 4043 6133
Size: 1064 138 724 601 49 906 174 2090
Cuts at: 6133 6507 6653 387
Size: 374 146 1280
Fragments arranged by size:
2090 1280 1064 906 724 601 374 174
146 138 49
EcoICRI GAG'CTC
Cuts at: 2120 2779 3673 3763 4033 4147 2120
Size: 659 894 90 270 114 5519
Fragments arranged by size:
5519 894 659 270 114 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2397 3335 3599 3659 3848 3869 4022
Size: 393 765 938 264 60 189 21 153
Cuts at: 4022 4139 5609 6206 6993 7261 1239
Size: 117 1470 597 787 268 1524
Fragments arranged by size:
1524 1470 938 787 765 597 393 268
264 189 153 117 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 4109 3317
Size: 792 6754
EcoO109I rG'GnC_Cy
Cuts at: 2643 2953 2954 3322 3737 4102 4328 4413 4555
Size: 310 1 368 415 365 226 85 142
Cuts at: 4555 2643
Size: 5634
Fragments arranged by size:
5634 415 368 365 310 226 142 85
1
EcoRI G'AATT_C
Cuts at: 4855 5233 4855
Size: 378 7168
FspI TGC'GCA
Cuts at: 3308 4364 6052 3308
Size: 1056 1688 4802
Fragments arranged by size:
4802 1688 1056
HaeII r_GCGC'y
Cuts at: 1678 2031 2333 2565 3011 4210 4409 4477 4713
Size: 353 302 232 446 1199 199 68 236
Cuts at: 4713 6693 1678
Size: 1980 2531
Fragments arranged by size:
2531 1980 1199 446 353 302 236 232
199 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2441 2473 4205
Size: 32 135 32 576 32 261 32 1732
Cuts at: 4205 4237 4283 4315 4445 4477 6187 6219 6261
Size: 32 46 32 130 32 1710 32 42
Cuts at: 6261 6293 1373
Size: 32 2626
Fragments arranged by size:
2626 1732 1710 576 261 135 130 46
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3176 4890 5136 669
Size: 988 281 1238 1714 246 3079
Fragments arranged by size:
3079 1714 1238 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3456 748
Size: 2708 4838
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KasI G'GCGC_C
Cuts at: 2329 4405 4709 2329
Size: 2076 304 5166
Fragments arranged by size:
5166 2076 304
KpnI G_GTAC'C
Cuts at: 4871 4871
Size: 7546
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 3912 4221 6660 6844 1305
Size: 1875 732 309 2439 184 2007
Fragments arranged by size:
2439 2007 1875 732 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3635 4547 10
Size: 54 1425 343 1803 912 3009
Fragments arranged by size:
3009 1803 1425 912 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4541 4727 5504 5863 6022 518
Size: 648 3375 186 777 359 159 2042
Fragments arranged by size:
3375 2042 777 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2227 2631 2733 2816 2828 3589 3955
Size: 457 164 404 102 83 12 761 366
Cuts at: 3955 4152 4168 4239 4374 4707 4962 5639 6787
Size: 197 16 71 135 333 255 677 1148
Cuts at: 6787 7032 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 761 677 457 404 366 333
255 245 197 164 135 102 83 71
16 12
NaeI GCC'GGC
Cuts at: 2194 2533 2194
Size: 339 7207
NarI GG'CG_CC
Cuts at: 2330 4406 4710 2330
Size: 2076 304 5166
Fragments arranged by size:
5166 2076 304
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6994
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7546
NgoMIV G'CCGG_C
Cuts at: 2192 2531 2192
Size: 339 7207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7546
NotI GC'GGCC_GC
Cuts at: 2823 4993 2823
Size: 2170 5376
NspI r_CATG'y
Cuts at: 1132 1229 2998 4204 4904 6449 1132
Size: 97 1769 1206 700 1545 2229
Fragments arranged by size:
2229 1769 1545 1206 700 97
PasI CC'CwG_GG
Cuts at: 1348 2583 1348
Size: 1235 6311
PciI A'CATG_T
Cuts at: 6445 6445
Size: 7546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2365 2589 3199 1108
Size: 1257 224 610 5455
Fragments arranged by size:
5455 1257 610 224
PfoI T'CCnGG_A
Cuts at: 2347 4599 2347
Size: 2252 5294
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2163 3657 3689 5615 5647 7128 7160 2131
Size: 32 1494 32 1926 32 1481 32 2517
Fragments arranged by size:
2517 1926 1494 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3322 4555 3322
Size: 1233 6313
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7546
PsiI TTA'TAA
Cuts at: 5116 5116
Size: 7546
PspOMI G'GGCC_C
Cuts at: 2953 4328 4412 2953
Size: 1375 84 6087
Fragments arranged by size:
6087 1375 84
PspXI vC'TCGA_Gb
Cuts at: 4679 4679
Size: 7546
PstI C_TGCA'G
Cuts at: 830 2068 2232 2772 3183 3234 3594 3954 4110
Size: 1238 164 540 411 51 360 360 156
Cuts at: 4110 4137 4173 4662 4898 830
Size: 27 36 489 236 3478
Fragments arranged by size:
3478 1238 540 489 411 360 360 236
164 156 51 36 27
PvuI CG_AT'CG
Cuts at: 2001 4438 2001
Size: 2437 5109
PvuII CAG'CTG
Cuts at: 2227 3589 3955 4168 2227
Size: 1362 366 213 5605
Fragments arranged by size:
5605 1362 366 213
RsrII CG'GwC_CG
Cuts at: 2426 2426
Size: 7546
SacI G_AGCT'C
Cuts at: 2122 2781 3675 3765 4035 4149 2122
Size: 659 894 90 270 114 5519
Fragments arranged by size:
5519 894 659 270 114 90
SacII CC_GC'GG
Cuts at: 2829 4240 4708 2829
Size: 1411 468 5667
Fragments arranged by size:
5667 1411 468
SalI G'TCGA_C
Cuts at: 1936 4888 1936
Size: 2952 4594
SbfI CC_TGCA'GG
Cuts at: 4110 4898 4110
Size: 788 6758
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SfcI C'TryA_G
Cuts at: 826 1045 2064 2228 2768 3179 3230 3590 3950
Size: 219 1019 164 540 411 51 360 360
Cuts at: 3950 4106 4133 4169 4658 4894 6029 6710 6901
Size: 156 27 36 489 236 1135 681 191
Cuts at: 6901 826
Size: 1471
Fragments arranged by size:
1471 1135 1019 681 540 489 411 360
360 236 219 191 164 156 51 36
27
SfoI GGC'GCC
Cuts at: 2331 4407 4711 2331
Size: 2076 304 5166
Fragments arranged by size:
5166 2076 304
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 4411 4873 1872
Size: 2539 462 4545
Fragments arranged by size:
4545 2539 462
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2795 3101 3326 3764 4679 5650
Size: 197 926 852 306 225 438 915 971
Cuts at: 5650 6551 6813 820
Size: 901 262 1553
Fragments arranged by size:
1553 971 926 915 901 852 438 306
262 225 197
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 2998 4904 1132
Size: 1866 1906 3774
Fragments arranged by size:
3774 1906 1866
SrfI GCCC'GGGC
Cuts at: 4411 4411
Size: 7546
SspI AAT'ATT
Cuts at: 5 52 4806 5470 5
Size: 47 4754 664 2081
Fragments arranged by size:
4754 2081 664 47
StuI AGG'CCT
Cuts at: 1517 3846 4643 1517
Size: 2329 797 4420
Fragments arranged by size:
4420 2329 797
StyI C'CwwG_G
Cuts at: 513 1065 4985 513
Size: 552 3920 3074
Fragments arranged by size:
3920 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2152 4112 5573 5590 5743 5928 6373
Size: 1255 572 1960 1461 17 153 185 445
Cuts at: 6373 325
Size: 1498
Fragments arranged by size:
1960 1498 1461 1255 572 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3681 4041 4047
Size: 274 80 33 51 1443 1704 360 6
Cuts at: 4047 4840 5792 7504 96
Size: 793 952 1712 138
Fragments arranged by size:
1712 1704 1443 952 793 360 274 138
80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7506 7538 7506
Size: 32 7514
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7546
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 7546
XhoI C'TCGA_G
Cuts at: 1943 4679 1943
Size: 2736 4810
XmaI C'CCGG_G
Cuts at: 1870 4409 4871 1870
Size: 2539 462 4545
Fragments arranged by size:
4545 2539 462
XmnI GAAnn'nnTTC
Cuts at: 4643 5675 4643
Size: 1032 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5354 276
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbvCI BfrBI BstZ17I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PsrI SanDI
SapI SexAI SfiI SwaI