Restriction map for
pFN21AA0406
(Circular) (Six-base) MAPSORT of: pFN21AA0406.seq Check: 5210 from: 1 to: 7972
pFN21AA0406 7972 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3815 3815
Size: 7972
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5782 278
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5315 1937
Size: 3378 4594
Acc65I G'GTAC_C
Cuts at: 5293 5293
Size: 7972
AclI AA'CG_TT
Cuts at: 1563 6099 6472 1563
Size: 4536 373 3063
Fragments arranged by size:
4536 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2132 2204 3023 4443 4673 4676 6035
Size: 491 9 72 819 1420 230 3 1359
Cuts at: 6035 7419 7687 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1420 1384 1359 819 491 268 230
72 9 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7775
AflIII A'CryG_T
Cuts at: 1203 2258 6871 7848 1203
Size: 1055 4613 977 1327
Fragments arranged by size:
4613 1327 1055 977
AgeI A'CCGG_T
Cuts at: 6803 6803
Size: 7972
AhdI GACnn_n'nnGTC
Cuts at: 2193 3124 6701 2193
Size: 931 3577 3464
Fragments arranged by size:
3577 3464 931
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 3162 1184
Size: 1227 751 5994
Fragments arranged by size:
5994 1227 751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4503 4535 4599 4631 4503
Size: 32 64 32 7844
Fragments arranged by size:
7844 64 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3602 4072 4972 5715 7287 3602
Size: 470 900 743 1572 4287
Fragments arranged by size:
4287 1572 900 743 470
ApaI G_GGCC'C
Cuts at: 3242 4979 3242
Size: 1737 6235
ApaLI G'TGCA_C
Cuts at: 6029 7185 6029
Size: 1156 6816
ApoI r'AATT_y
Cuts at: 1493 3194 4084 5281 5507 5659 7768 1493
Size: 1701 890 1197 226 152 2109 1697
Fragments arranged by size:
2109 1701 1697 1197 890 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2393 6526 160
Size: 872 1361 4133 1606
Fragments arranged by size:
4133 1606 1361 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
AvaI C'yCGr_G
Cuts at: 1870 1943 5297 1870
Size: 73 3354 4545
Fragments arranged by size:
4545 3354 73
AvrII C'CTAG_G
Cuts at: 4999 4999
Size: 7972
BamHI G'GATC_C
Cuts at: 5302 5669 5302
Size: 367 7605
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4338 5293 6748 618
Size: 325 217 627 2551 955 1455 1842
Fragments arranged by size:
2551 1842 1455 955 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2439 3242 4979 5291 1391
Size: 1048 803 1737 312 4072
Fragments arranged by size:
4072 1737 1048 803 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2192 4431 5086 928
Size: 971 293 2239 655 3814
Fragments arranged by size:
3814 2239 971 655 293
BbvCI CC'TCA_GC
Cuts at: 3391 3391
Size: 7972
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5227 5261 6163 6197 1903
Size: 34 3290 34 902 34 3678
Fragments arranged by size:
3678 3290 902 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5864 7074 5864
Size: 1210 6762
BclI T'GATC_A
Cuts at: 1548 4074 1548
Size: 2526 5446
BfrBI ATG'CAT
Cuts at: 3888 3888
Size: 7972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4775 6583 136
Size: 107 122 71 631 605 3103 1808 1525
Fragments arranged by size:
3103 1808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2715 7967 2715
Size: 5252 2720
BlpI GC'TnA_GC
Cuts at: 5389 5389
Size: 7972
Bme1580I G_kGCm'C
Cuts at: 1790 2137 3242 4341 4979 6033 7189 1790
Size: 347 1105 1099 638 1054 1156 2573
Fragments arranged by size:
2573 1156 1105 1099 1054 638 347
BmgBI CAC'GTC
Cuts at: 1318 3322 3792 4600 1318
Size: 2004 470 808 4690
Fragments arranged by size:
4690 2004 808 470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3046 4303 5703
Size: 416 53 83 399 24 1629 1257 1400
Cuts at: 5703 6661 442
Size: 958 1753
Fragments arranged by size:
1753 1629 1400 1257 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 3263 3632 3995 4005 5234 6632 1239
Size: 1028 996 369 363 10 1229 1398 2579
Fragments arranged by size:
2579 1398 1229 1028 996 369 363 10
Bpu10I CC'TnA_GC
Cuts at: 3391 3391
Size: 7972
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3487 4421 5243 6097 6962 7260 3487
Size: 934 822 854 865 298 4199
Fragments arranged by size:
4199 934 865 854 822 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3981 6635 882
Size: 617 2482 2654 2219
Fragments arranged by size:
2654 2482 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4805 5763 493
Size: 711 3601 958 2702
Fragments arranged by size:
3601 2702 958 711
BsaBI GATnn'nnATC
Cuts at: 4635 7966 4635
Size: 3331 4641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4767 5779 6161 6796
Size: 53 83 186 936 3234 1012 382 635
Cuts at: 6796 275
Size: 1451
Fragments arranged by size:
3234 1451 1012 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3558 6405 6803 7077 7224 7635 3558
Size: 2847 398 274 147 411 3895
Fragments arranged by size:
3895 2847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5108 5138 1178
Size: 30 165 30 3705 30 4012
Fragments arranged by size:
4012 3705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2369 3436 3767 1186
Size: 856 327 1067 331 5391
Fragments arranged by size:
5391 1067 856 331 327
BseYI C'CCAG_C
Cuts at: 2324 2608 3605 5183 7175 7671 2324
Size: 284 997 1578 1992 496 2625
Fragments arranged by size:
2625 1992 1578 997 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2634 2986 5183 5189 5219 1163
Size: 573 898 352 2197 6 30 3916
Fragments arranged by size:
3916 2197 898 573 352 30 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5422 6183 7211 1540
Size: 275 186 3421 761 1028 2301
Fragments arranged by size:
3421 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 3771 4412 5250 6033 6118 7189 2802
Size: 969 641 838 783 85 1071 3585
Fragments arranged by size:
3585 1071 969 838 783 641 85
BsmI GAATG_Cn'
Cuts at: 2237 3139 3379 3888 5483 5576 2237
Size: 902 240 509 1595 93 4633
Fragments arranged by size:
4633 1595 902 509 240 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2439 2802 3242 3771 4341 4412
Size: 399 347 302 363 440 529 570 71
Cuts at: 4412 4979 5250 5291 6033 6118 7189 1391
Size: 567 271 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 567 529 440 399
363 347 302 271 85 71 41
BspEI T'CCGG_A
Cuts at: 7635 7635
Size: 7972
BspHI T'CATG_A
Cuts at: 5861 7591 5861
Size: 1730 6242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2347 2797 3463 3815 4200 5260 5327 844
Size: 1503 450 666 352 385 1060 67 3489
Fragments arranged by size:
3489 1503 1060 666 450 385 352 67
BsrBI CCG'CTC
Cuts at: 1394 1823 5859 1394
Size: 429 4036 3507
Fragments arranged by size:
4036 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 6467 6641 65
Size: 1183 3326 1893 174 1396
Fragments arranged by size:
3326 1893 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4842 6616 6803 1700
Size: 110 3032 1774 187 2869
Fragments arranged by size:
3032 2869 1774 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7972
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7972
BssSI C'ACGA_G
Cuts at: 6032 7044 6032
Size: 1012 6960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2789 794
Size: 1995 5977
BstBI TT'CG_AA
Cuts at: 6776 6776
Size: 7972
BstEII G'GTnAC_C
Cuts at: 1184 3721 5009 1184
Size: 2537 1288 4147
Fragments arranged by size:
4147 2537 1288
BstYI r'GATC_y
Cuts at: 1972 2715 3622 4107 4581 4672 5302 5669 6054
Size: 743 907 485 474 91 630 367 385
Cuts at: 6054 6071 7523 7609 7621 7967 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 907 743 630 485 474 385
367 346 91 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 2025
Size: 7972
BtgI C'CryG_G
Cuts at: 513 1065 2004 4593 513
Size: 552 939 2589 3892
Fragments arranged by size:
3892 2589 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7233
Fragments arranged by size:
7233 683 56
BtsI GCAGTG_nn'
Cuts at: 2561 4568 4785 5488 6282 6302 2561
Size: 2007 217 703 794 20 4231
Fragments arranged by size:
4231 2007 794 703 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4557 4592 529
Size: 35 3993 35 3909
Fragments arranged by size:
3993 3909 35 35
DraI TTT'AAA
Cuts at: 2680 4116 5276 2680
Size: 1436 1160 5376
Fragments arranged by size:
5376 1436 1160
DraIII CAC_nnn'GTG
Cuts at: 5197 5197
Size: 7972
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6979 809
Size: 507 5663 1802
Fragments arranged by size:
5663 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4230 5419
Size: 54 1425 50 275 18 2077 323 1189
Cuts at: 5419 6308 7937 8
Size: 889 1629 43
Fragments arranged by size:
2077 1629 1425 1189 889 323 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5419 1537
Size: 275 3607 4090
Fragments arranged by size:
4090 3607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 3162 4359 4400 4500 5902 1353
Size: 815 994 1197 41 100 1402 3423
Fragments arranged by size:
3423 1402 1197 994 815 100 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4637 6559 6933 7079 387
Size: 1064 138 3048 1922 374 146 1280
Fragments arranged by size:
3048 1922 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2132 2204 2267 3023 3263 3632
Size: 393 491 9 72 63 756 240 369
Cuts at: 3632 3995 4005 4443 4673 4676 5234 6035 6632
Size: 363 10 438 230 3 558 801 597
Cuts at: 6632 7419 7687 1239
Size: 787 268 1524
Fragments arranged by size:
1524 801 787 756 597 558 491 438
393 369 363 268 240 230 72 63
10 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 5003 3002
Size: 2001 5971
EcoO109I rG'GnC_Cy
Cuts at: 2195 2950 4156 4894 4969 4976 2195
Size: 755 1206 738 75 7 5191
Fragments arranged by size:
5191 1206 755 738 75 7
EcoRI G'AATT_C
Cuts at: 5281 5659 5281
Size: 378 7594
FspI TGC'GCA
Cuts at: 6478 6478
Size: 7972
HaeII r_GCGC'y
Cuts at: 1678 7119 1678
Size: 5441 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 3110 3142 4499
Size: 32 135 32 607 32 899 32 1357
Cuts at: 4499 4531 5108 5140 6613 6645 6687 6719 1373
Size: 32 577 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 1357 899 607 577 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 5316 5562 669
Size: 988 281 927 2451 246 3079
Fragments arranged by size:
3079 2451 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7972
HpaI GTT'AAC
Cuts at: 5562 5562
Size: 7972
KpnI G_GTAC'C
Cuts at: 5297 5297
Size: 7972
MfeI C'AATT_G
Cuts at: 5571 5571
Size: 7972
MluI A'CGCG_T
Cuts at: 7848 7848
Size: 7972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3335 3876 7086 7270 1305
Size: 2030 541 3210 184 2007
Fragments arranged by size:
3210 2030 2007 541 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3909 4232 10
Size: 54 1425 343 2077 323 3750
Fragments arranged by size:
3750 2077 1425 343 323 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 4028 4598 5930 6289 6448 518
Size: 648 1227 1635 570 1332 359 159 2042
Fragments arranged by size:
2042 1635 1332 1227 648 570 359 159
MspA1I CmG'CkG
Cuts at: 1606 2608 3283 4421 4711 4954 5203 5217 5254
Size: 1002 675 1138 290 243 249 14 37
Cuts at: 5254 5388 6065 7213 7458 1606
Size: 134 677 1148 245 2120
Fragments arranged by size:
2120 1148 1138 1002 677 675 290 249
245 243 134 37 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 4593 513
Size: 552 939 2589 3892
Fragments arranged by size:
3892 2589 939 552
NdeI CA'TA_TG
Cuts at: 387 3919 387
Size: 3532 4440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7972
NotI GC'GGCC_GC
Cuts at: 5419 5419
Size: 7972
NsiI A_TGCA'T
Cuts at: 3890 3890
Size: 7972
NspI r_CATG'y
Cuts at: 1132 1229 2262 4289 5330 6875 1132
Size: 97 1033 2027 1041 1545 2229
Fragments arranged by size:
2229 2027 1545 1041 1033 97
PasI CC'CwG_GG
Cuts at: 1348 4810 1348
Size: 3462 4510
PciI A'CATG_T
Cuts at: 2258 6871 2258
Size: 4613 3359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 2224 1108
Size: 975 141 6856
Fragments arranged by size:
6856 975 141
PfoI T'CCnGG_A
Cuts at: 2283 4158 2283
Size: 1875 6097
PmeI GTTT'AAAC
Cuts at: 5276 5276
Size: 7972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6041 6073 7554 7586 6041
Size: 32 1481 32 6427
Fragments arranged by size:
6427 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2950 4156 2195
Size: 755 1206 6011
Fragments arranged by size:
6011 1206 755
PshAI GACnn'nnGTC
Cuts at: 1613 4548 1613
Size: 2935 5037
PsiI TTA'TAA
Cuts at: 5542 5542
Size: 7972
PspOMI G'GGCC_C
Cuts at: 3238 4975 3238
Size: 1737 6235
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5135 5167 5135
Size: 32 7940
PstI C_TGCA'G
Cuts at: 830 2106 2794 4068 5271 5324 830
Size: 1276 688 1274 1203 53 3478
Fragments arranged by size:
3478 1276 1274 1203 688 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7972
PvuII CAG'CTG
Cuts at: 2608 4421 4711 4954 5203 5254 2608
Size: 1813 290 243 249 51 5326
Fragments arranged by size:
5326 1813 290 249 243 51
SalI G'TCGA_C
Cuts at: 1936 5314 1936
Size: 3378 4594
SapI GCTCTTCn'nnn_
Cuts at: 4400 4400
Size: 7972
SbfI CC_TGCA'GG
Cuts at: 5324 5324
Size: 7972
ScaI AGT'ACT
Cuts at: 3972 6220 7932 3972
Size: 2248 1712 4012
Fragments arranged by size:
4012 2248 1712
SexAI A'CCwGG_T
Cuts at: 5129 5129
Size: 7972
SfcI C'TryA_G
Cuts at: 826 1045 2102 2790 4064 5267 5320 6455 7136
Size: 219 1057 688 1274 1203 53 1135 681
Cuts at: 7136 7327 826
Size: 191 1471
Fragments arranged by size:
1471 1274 1203 1135 1057 688 681 219
191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7972
SmaI CCC'GGG
Cuts at: 1872 5299 1872
Size: 3427 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3466 4400 5258 6076 6977 7239
Size: 197 926 1523 934 858 818 901 262
Cuts at: 7239 820
Size: 1553
Fragments arranged by size:
1553 1523 934 926 901 858 818 262
197
SnaBI TAC'GTA
Cuts at: 493 4805 5763 493
Size: 4312 958 2702
Fragments arranged by size:
4312 2702 958
SpeI A'CTAG_T
Cuts at: 152 3019 152
Size: 2867 5105
SphI G_CATG'C
Cuts at: 1132 4289 5330 1132
Size: 3157 1041 3774
Fragments arranged by size:
3774 3157 1041
SspI AAT'ATT
Cuts at: 5 52 5896 5
Size: 47 5844 2081
Fragments arranged by size:
5844 2081 47
StuI AGG'CCT
Cuts at: 1517 2033 2484 3008 3777 4733 1517
Size: 516 451 524 769 956 4756
Fragments arranged by size:
4756 956 769 524 516 451
StyI C'CwwG_G
Cuts at: 513 1065 2004 2576 3404 4593 4719 4999 5411
Size: 552 939 572 828 1189 126 280 412
Cuts at: 5411 513
Size: 3074
Fragments arranged by size:
3074 1189 939 828 572 552 412 280
126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2922 3786 4904 5999 6016 6169
Size: 1255 758 584 864 1118 1095 17 153
Cuts at: 6169 6354 6799 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1118 1095 864 758 584 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 3970 6218
Size: 274 80 33 51 1443 177 1816 2248
Cuts at: 6218 7930 96
Size: 1712 138
Fragments arranged by size:
2248 1816 1712 1443 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3142 3174 7932 7964 3142
Size: 32 4758 32 3150
Fragments arranged by size:
4758 3150 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7972
XbaI T'CTAG_A
Cuts at: 3310 5308 3310
Size: 1998 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4027 4600 5127 4027
Size: 573 527 6872
Fragments arranged by size:
6872 573 527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7972
XmaI C'CCGG_G
Cuts at: 1870 5297 1870
Size: 3427 4545
XmnI GAAnn'nnTTC
Cuts at: 6101 6101
Size: 7972
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5780 276
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BplI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI