Restriction map for
pFN21AA0405
(Circular) (Six-base) MAPSORT of: pFN21AA0405.seq Check: 4982 from: 1 to: 6685
pFN21AA0405 6685 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3605 4495 278
Size: 53 83 186 936 2069 890 2468
Fragments arranged by size:
2468 2069 936 890 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 2283 4028 1937
Size: 208 138 1745 4594
Fragments arranged by size:
4594 1745 208 138
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2665 2930 4748 6132 6400 1632
Size: 440 593 265 1818 1384 268 1917
Fragments arranged by size:
1917 1818 1384 593 440 268 265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 5584 6561 1203
Size: 4381 977 1327
Fragments arranged by size:
4381 1327 977
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 3365 1166
Size: 2199 4486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2937 1184
Size: 1753 4932
AlwNI CAG_nnn'CTG
Cuts at: 4428 6000 4428
Size: 1572 5113
ApaLI G'TGCA_C
Cuts at: 2273 4742 5898 2273
Size: 2469 1156 3060
Fragments arranged by size:
3060 2469 1156
ApoI r'AATT_y
Cuts at: 1493 2309 2705 3994 4220 4372 6481 1493
Size: 816 396 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 816 396 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2229 3906 5239 160
Size: 872 1197 1677 1333 1606
Fragments arranged by size:
1677 1606 1333 1197 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 4010 1870
Size: 73 2067 4545
Fragments arranged by size:
4545 2067 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2647 2680 2647
Size: 33 6652
BamHI G'GATC_C
Cuts at: 2184 3276 4015 4382 2184
Size: 1092 739 367 4487
Fragments arranged by size:
4487 1092 739 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3779 4006 5461 618
Size: 325 217 627 888 1104 227 1455 1842
Fragments arranged by size:
1842 1455 1104 888 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2082 3459 3620 4004 1391
Size: 691 1377 161 384 4072
Fragments arranged by size:
4072 1377 691 384 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 3525 928
Size: 971 564 1062 4088
Fragments arranged by size:
4088 1062 971 564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 2766 1548
Size: 1218 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2533 5296 136
Size: 107 122 71 631 605 861 2763 1525
Fragments arranged by size:
2763 1525 861 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6680 6680
Size: 6685
BlpI GC'TnA_GC
Cuts at: 3680 4102 3680
Size: 422 6263
Bme1580I G_kGCm'C
Cuts at: 1790 2277 2678 4746 5902 1790
Size: 487 401 2068 1156 2573
Fragments arranged by size:
2573 2068 1156 487 401
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3244 4416 5374
Size: 416 53 83 399 24 1827 1172 958
Cuts at: 5374 442
Size: 1753
Fragments arranged by size:
1827 1753 1172 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3824 3989 5345 1239
Size: 2585 165 1356 2579
Fragments arranged by size:
2585 2579 1356 165
Bpu10I CC'TnA_GC
Cuts at: 2516 3437 2516
Size: 921 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 2865 4810 5675 5973 2684
Size: 181 1945 865 298 3396
Fragments arranged by size:
3396 1945 865 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5348 882
Size: 617 3849 2219
Fragments arranged by size:
3849 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2720 4476 493
Size: 711 1516 1756 2702
Fragments arranged by size:
2702 1756 1516 711
BsaBI GATnn'nnATC
Cuts at: 2571 6679 2571
Size: 4108 2577
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3602 4492 4874 5509
Size: 53 83 186 936 2069 890 382 635
Cuts at: 5509 275
Size: 1451
Fragments arranged by size:
2069 1451 936 890 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5118 5516 5790 5937 6348 5118
Size: 398 274 147 411 5455
Fragments arranged by size:
5455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 3121 3136 3151
Size: 30 165 30 793 30 895 15 15
Cuts at: 3151 3166 1178
Size: 15 4697
Fragments arranged by size:
4697 895 793 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2730 1186
Size: 1544 5141
BseYI C'CCAG_C
Cuts at: 2030 2394 3118 5888 6384 2030
Size: 364 724 2770 496 2331
Fragments arranged by size:
2770 2331 724 496 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 2402 1163
Size: 573 504 162 5446
Fragments arranged by size:
5446 573 504 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3975 4746 4831 5902 2277
Size: 1698 771 85 1071 3060
Fragments arranged by size:
3060 1698 1071 771 85
BsmI GAATG_Cn'
Cuts at: 4196 4289 4196
Size: 93 6592
BsmBI CGTCTCn'nnnn_
Cuts at: 3838 3838
Size: 6685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2277 2678 3459 3620 3975 4004
Size: 399 292 195 401 781 161 355 29
Cuts at: 4004 4746 4831 5902 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 401 399 355 292
195 161 85 29
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 4574 6304 4574
Size: 1730 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4040 844
Size: 3196 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4572 1394
Size: 429 2749 3507
Fragments arranged by size:
3507 2749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5180 5354 65
Size: 1183 3932 174 1396
Fragments arranged by size:
3932 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3750 5329 5516 1700
Size: 110 1940 1579 187 2869
Fragments arranged by size:
2869 1940 1579 187 110
BsrGI T'GTAC_A
Cuts at: 96 2263 96
Size: 2167 4518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 2614 4745 5757 2614
Size: 2131 1012 3542
Fragments arranged by size:
3542 2131 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6685
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2104
Size: 6685
BstYI r'GATC_y
Cuts at: 1972 2184 3276 4015 4382 4767 4784 6236 6322
Size: 212 1092 739 367 385 17 1452 86
Cuts at: 6322 6334 6680 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1092 739 385 367 346 212
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2035 513
Size: 552 939 31 5163
Fragments arranged by size:
5163 939 552 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5946
Fragments arranged by size:
5946 683 56
BtsI GCAGTG_nn'
Cuts at: 2527 2919 3973 4201 4995 5015 2527
Size: 392 1054 228 794 20 4197
Fragments arranged by size:
4197 1054 794 392 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6650
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DraIII CAC_nnn'GTG
Cuts at: 2025 2025
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5692 809
Size: 507 4376 1802
Fragments arranged by size:
4376 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3048 4132 5021
Size: 54 1425 50 275 18 1218 1084 889
Cuts at: 5021 6650 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1218 1084 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 3116 3341 4615 1353
Size: 1053 710 225 1274 3423
Fragments arranged by size:
3423 1274 1053 710 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3769 5272 5646 5792 387
Size: 1064 138 2180 1503 374 146 1280
Fragments arranged by size:
2180 1503 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2665 2930 3824 3989 4748 5345
Size: 393 440 593 265 894 165 759 597
Cuts at: 5345 6132 6400 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 787 759 597 593 440 393
268 265 165
EcoO109I rG'GnC_Cy
Cuts at: 2463 3026 2463
Size: 563 6122
EcoRI G'AATT_C
Cuts at: 2309 3994 4372 2309
Size: 1685 378 4622
Fragments arranged by size:
4622 1685 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3150 3150
Size: 6685
FspI TGC'GCA
Cuts at: 5191 5191
Size: 6685
HaeII r_GCGC'y
Cuts at: 1678 3718 5832 1678
Size: 2040 2114 2531
Fragments arranged by size:
2531 2114 2040
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2863 2885 2895
Size: 32 135 32 845 32 414 22 10
Cuts at: 2895 2917 3446 3478 5326 5358 5400 5432 1373
Size: 22 529 32 1848 32 42 32 2626
Fragments arranged by size:
2626 1848 845 529 414 135 42 32
32 32 32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3448 4029 4275 669
Size: 988 281 1510 581 246 3079
Fragments arranged by size:
3079 1510 988 581 281 246
HindIII A'AGCT_T
Cuts at: 748 2161 2405 748
Size: 1413 244 5028
Fragments arranged by size:
5028 1413 244
HpaI GTT'AAC
Cuts at: 3448 4275 3448
Size: 827 5858
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 2712 4284 2712
Size: 1572 5113
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2616 2880 2928 3164 3221 5799 5983 1305
Size: 1311 264 48 236 57 2578 184 2007
Fragments arranged by size:
2578 2007 1311 264 236 184 57 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4863
Fragments arranged by size:
4863 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 3344 3365 4643 5002 5161 518
Size: 648 1258 920 21 1278 359 159 2042
Fragments arranged by size:
2042 1278 1258 920 648 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2127 3324 3420 4101 4778 5926 6171 1606
Size: 521 1197 96 681 677 1148 245 2120
Fragments arranged by size:
2120 1197 1148 681 677 521 245 96
NaeI GCC'GGC
Cuts at: 3752 3752
Size: 6685
NcoI C'CATG_G
Cuts at: 513 1065 2004 2035 513
Size: 552 939 31 5163
Fragments arranged by size:
5163 939 552 31
NdeI CA'TA_TG
Cuts at: 387 3699 387
Size: 3312 3373
NgoMIV G'CCGG_C
Cuts at: 3750 3750
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 3940 4043 5588 1132
Size: 97 2711 103 1545 2229
Fragments arranged by size:
2711 2229 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 2074 1348
Size: 726 5959
PciI A'CATG_T
Cuts at: 5584 5584
Size: 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2025 3613 1108
Size: 917 1588 4180
Fragments arranged by size:
4180 1588 917
PfoI T'CCnGG_A
Cuts at: 2187 2469 3801 2187
Size: 282 1332 5071
Fragments arranged by size:
5071 1332 282
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3026 3026
Size: 6685
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 6653
PstI C_TGCA'G
Cuts at: 830 2251 2954 3879 4037 830
Size: 1421 703 925 158 3478
Fragments arranged by size:
3478 1421 925 703 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
PvuII CAG'CTG
Cuts at: 3324 3324
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SapI GCTCTTCn'nnn_
Cuts at: 2406 2406
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SfcI C'TryA_G
Cuts at: 826 1045 2012 2247 2950 3875 4033 5168 5849
Size: 219 967 235 703 925 158 1135 681
Cuts at: 5849 6040 826
Size: 191 1471
Fragments arranged by size:
1471 1135 967 925 703 681 235 219
191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 2880 4789 5690 5952 820
Size: 197 926 756 181 1909 901 262 1553
Fragments arranged by size:
1909 1553 926 901 756 262 197 181
SnaBI TAC'GTA
Cuts at: 493 2720 4476 493
Size: 2227 1756 2702
Fragments arranged by size:
2702 2227 1756
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 4043 1132
Size: 2911 3774
SspI AAT'ATT
Cuts at: 5 52 2365 4609 5
Size: 47 2313 2244 2081
Fragments arranged by size:
2313 2244 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2004 2035 2936 3022 4124 513
Size: 552 939 31 901 86 1102 3074
Fragments arranged by size:
3074 1102 939 901 552 86 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3355 3560 4712 4729 4882 5067 5512
Size: 1255 1775 205 1152 17 153 185 445
Cuts at: 5512 325
Size: 1498
Fragments arranged by size:
1775 1498 1255 1152 445 205 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2263 3128 4931
Size: 274 80 33 51 1443 286 865 1803
Cuts at: 4931 6643 96
Size: 1712 138
Fragments arranged by size:
1803 1712 1443 865 286 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3136 3168 6645 6677 3136
Size: 32 3477 32 3144
Fragments arranged by size:
3477 3144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3601 1316
Size: 2285 4400
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2449 2443
Size: 6 6679
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 4814 4814
Size: 6685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3603 4493 276
Size: 53 83 186 936 2069 890 2468
Fragments arranged by size:
2468 2069 936 890 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI