Restriction map for
pFN21AA0404
(Circular) (Six-base) MAPSORT of: pFN21AA0404.seq Check: 1710 from: 1 to: 10519
pFN21AA0404 10519 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2871 4103 6238 2871
Size: 1232 2135 7152
Fragments arranged by size:
7152 2135 1232
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3329 8329 278
Size: 53 83 186 936 1793 5000 2468
Fragments arranged by size:
5000 2468 1793 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 4491 5943 7862 1937
Size: 1228 1326 1452 1919 4594
Fragments arranged by size:
4594 1919 1452 1326 1228
Acc65I G'GTAC_C
Cuts at: 4898 7840 4898
Size: 2942 7577
AclI AA'CG_TT
Cuts at: 1563 8646 9019 1563
Size: 7083 373 3063
Fragments arranged by size:
7083 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3305 4823 4891 5889 6979 7145 8582
Size: 1247 426 1518 68 998 1090 166 1437
Cuts at: 8582 9966 10234 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1518 1437 1384 1247 1090 998 426
268 166 68
AfeI AGC'GCT
Cuts at: 6690 7502 6690
Size: 812 9707
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10322
AflIII A'CryG_T
Cuts at: 1203 5136 5659 9418 10395 1203
Size: 3933 523 3759 977 1327
Fragments arranged by size:
3933 3759 1327 977 523
AgeI A'CCGG_T
Cuts at: 7410 9350 7410
Size: 1940 8579
AhdI GACnn_n'nnGTC
Cuts at: 3516 7459 9248 3516
Size: 3943 1789 4787
Fragments arranged by size:
4787 3943 1789
AleI CACnn'nnGTG
Cuts at: 1166 2528 3485 5150 5670 6257 6639 1166
Size: 1362 957 1665 520 587 382 5046
Fragments arranged by size:
5046 1665 1362 957 587 520 382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3503 3563 5688 6233 1184
Size: 2319 60 2125 545 5470
Fragments arranged by size:
5470 2319 2125 545 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 10487
AlwNI CAG_nnn'CTG
Cuts at: 2348 2452 2596 2902 3004 3016 3035 3694 6515
Size: 104 144 306 102 12 19 659 2821
Cuts at: 6515 6535 6580 7388 8262 9834 2348
Size: 20 45 808 874 1572 3033
Fragments arranged by size:
3033 2821 1572 874 808 659 306 144
104 102 45 20 19 12
ApaI G_GGCC'C
Cuts at: 3402 3802 5974 6002 7253 3402
Size: 400 2172 28 1251 6668
Fragments arranged by size:
6668 2172 1251 400 28
ApaLI G'TGCA_C
Cuts at: 3548 4865 5663 8576 9732 3548
Size: 1317 798 2913 1156 4335
Fragments arranged by size:
4335 2913 1317 1156 798
ApoI r'AATT_y
Cuts at: 1493 7828 8054 8206 10315 1493
Size: 6335 226 152 2109 1697
Fragments arranged by size:
6335 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9073 160
Size: 872 8041 1606
Fragments arranged by size:
8041 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10519
AvaI C'yCGr_G
Cuts at: 1870 1943 2875 3411 3588 3921 4582 5104 5246
Size: 73 932 536 177 333 661 522 142
Cuts at: 5246 6126 6255 6869 6876 7739 7844 1870
Size: 880 129 614 7 863 105 4545
Fragments arranged by size:
4545 932 880 863 661 614 536 522
333 177 142 129 105 73 7
BamHI G'GATC_C
Cuts at: 7849 8216 7849
Size: 367 10152
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 2972 3593 4898 6226
Size: 325 217 627 848 337 621 1305 1328
Cuts at: 6226 6288 7655 7840 9295 618
Size: 62 1367 185 1455 1842
Fragments arranged by size:
1842 1455 1367 1328 1305 848 627 621
337 325 217 185 62
BanII G_rGCy'C
Cuts at: 1391 2240 2433 2721 2748 2855 3042 3150 3402
Size: 849 193 288 27 107 187 108 252
Cuts at: 3402 3647 3802 3876 4032 4326 5974 6002 6365
Size: 245 155 74 156 294 1648 28 363
Cuts at: 6365 6883 7158 7253 7838 1391
Size: 518 275 95 585 4072
Fragments arranged by size:
4072 1648 849 585 518 363 294 288
275 252 245 193 187 156 155 108
107 95 74 28 27
BbeI G_GCGC'C
Cuts at: 2639 7659 2639
Size: 5020 5499
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 4754 928
Size: 971 1086 1769 6693
Fragments arranged by size:
6693 1769 1086 971
BbvCI CC'TCA_GC
Cuts at: 2102 3044 3133 3655 6858 2102
Size: 942 89 522 3203 5763
Fragments arranged by size:
5763 3203 942 522 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3365 3399 5666 5700 8710 8744 1903
Size: 34 1428 34 2267 34 3010 34 3678
Fragments arranged by size:
3678 3010 2267 1428 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7588 8411 9621 7588
Size: 823 1210 8486
Fragments arranged by size:
8486 1210 823
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10519
BfrBI ATG'CAT
Cuts at: 2376 2376
Size: 10519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3998 4602 4751
Size: 107 122 71 631 605 2326 604 149
Cuts at: 4751 5115 6006 6760 7721 9130 136
Size: 364 891 754 961 1409 1525
Fragments arranged by size:
2326 1525 1409 961 891 754 631 605
604 364 149 122 107 71
BglII A'GATC_T
Cuts at: 4546 10514 4546
Size: 5968 4551
BlpI GC'TnA_GC
Cuts at: 2117 2906 3036 3846 4002 4049 5049 6461 7936
Size: 789 130 810 156 47 1000 1412 1475
Cuts at: 7936 2117
Size: 4700
Fragments arranged by size:
4700 1475 1412 1000 810 789 156 130
47
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3402 3482 3552 3596 3802 4869 5667
Size: 469 1143 80 70 44 206 1067 798
Cuts at: 5667 5689 5816 5974 6002 6229 6249 6664 7076
Size: 22 127 158 28 227 20 415 412
Cuts at: 7076 7253 7813 8580 9736 1790
Size: 177 560 767 1156 2573
Fragments arranged by size:
2573 1156 1143 1067 798 767 560 469
415 412 227 206 177 158 127 80
70 44 28 22 20
BmgBI CAC'GTC
Cuts at: 1318 3787 5287 6659 7014 7054 7618 7728 1318
Size: 2469 1500 1372 355 40 564 110 4109
Fragments arranged by size:
4109 2469 1500 1372 564 355 110 40
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4027 4064 4194
Size: 416 53 83 399 24 2610 37 130
Cuts at: 4194 4225 4452 4912 5007 5025 5260 5384 5813
Size: 31 227 460 95 18 235 124 429
Cuts at: 5813 6236 8250 9208 442
Size: 423 2014 958 1753
Fragments arranged by size:
2610 2014 1753 958 460 429 423 416
399 235 227 130 124 95 83 53
37 31 24 18
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6614 6614
Size: 10519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2234 2291 2474 2483 2618 2724 2870
Size: 974 21 57 183 9 135 106 146
Cuts at: 2870 3584 3593 3837 4037 4301 4401 4877 5413
Size: 714 9 244 200 264 100 476 536
Cuts at: 5413 5690 6557 6602 9179 1239
Size: 277 867 45 2577 2579
Fragments arranged by size:
2579 2577 974 867 714 536 476 277
264 244 200 183 146 135 106 100
57 45 21 9 9
Bpu10I CC'TnA_GC
Cuts at: 2102 2770 3044 3133 3655 3966 4320 5465 6013
Size: 668 274 89 522 311 354 1145 548
Cuts at: 6013 6858 2102
Size: 845 5763
Fragments arranged by size:
5763 1145 845 668 548 522 354 311
274 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6779 7775 8644 9509 9807 6779
Size: 996 869 865 298 7491
Fragments arranged by size:
7491 996 869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4377 4823 5408 5513 6280 6585 6842
Size: 617 2878 446 585 105 767 305 257
Cuts at: 6842 6917 7436 9182 882
Size: 75 519 1746 2219
Fragments arranged by size:
2878 2219 1746 767 617 585 519 446
305 257 105 75
BsaAI yAC'GTr
Cuts at: 493 1204 5662 5713 6269 8310 493
Size: 711 4458 51 556 2041 2702
Fragments arranged by size:
4458 2702 2041 711 556 51
BsaBI GATnn'nnATC
Cuts at: 2652 4373 10513 2652
Size: 1721 6140 2658
Fragments arranged by size:
6140 2658 1721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2636 3326 5859 5927
Size: 53 83 186 936 1103 690 2533 68
Cuts at: 5927 7656 7770 8326 8708 9343 275
Size: 1729 114 556 382 635 1451
Fragments arranged by size:
2533 1729 1451 1103 936 690 635 556
382 186 114 83 68 53
BsaWI w'CCGG_w
Cuts at: 2425 3341 4038 7410 8952 9350 9624 9771 10182
Size: 916 697 3372 1542 398 274 147 411
Cuts at: 10182 2425
Size: 2762
Fragments arranged by size:
3372 2762 1542 916 697 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3158 3182 3188 3212 3566
Size: 30 165 30 1755 24 6 24 354
Cuts at: 3566 3596 5339 5369 5370 5400 6950 6980 7712
Size: 30 1743 30 1 30 1550 30 732
Cuts at: 7712 7742 1178
Size: 30 3955
Fragments arranged by size:
3955 1755 1743 1550 732 354 165 30
30 30 30 30 30 30 24 24
6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 3166 4327 4379 4406 6044 6068 6554
Size: 1543 437 1161 52 27 1638 24 486
Cuts at: 6554 6595 7079 1186
Size: 41 484 4626
Fragments arranged by size:
4626 1638 1543 1161 486 484 437 52
41 27 24
BseYI C'CCAG_C
Cuts at: 2364 2848 3226 3640 3793 4478 4575 5305 5753
Size: 484 378 414 153 685 97 730 448
Cuts at: 5753 5898 6248 6493 6524 6882 6887 7177 7237
Size: 145 350 245 31 358 5 290 60
Cuts at: 7237 9722 10218 2364
Size: 2485 496 2665
Fragments arranged by size:
2665 2485 730 685 496 484 448 414
378 358 350 290 245 153 145 97
60 31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2269 2609 2657 2690 2843 3509
Size: 573 315 218 340 48 33 153 666
Cuts at: 3509 3900 5324 5717 6593 1163
Size: 391 1424 393 876 5089
Fragments arranged by size:
5089 1424 876 666 573 393 391 340
315 218 153 48 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4992 6200 6204 6548 7969 8730
Size: 275 186 2991 1208 4 344 1421 761
Cuts at: 8730 9758 1540
Size: 1028 2301
Fragments arranged by size:
2991 2301 1421 1208 1028 761 344 275
186 4
BsiHKAI G_wGCw'C
Cuts at: 2100 2544 2721 3150 3552 4184 4778 4869 4896
Size: 444 177 429 402 632 594 91 27
Cuts at: 4896 5603 5667 5723 6365 8580 8665 9736 2100
Size: 707 64 56 642 2215 85 1071 2883
Fragments arranged by size:
2883 2215 1071 707 642 632 594 444
429 402 177 91 85 64 56 27
BsmI GAATG_Cn'
Cuts at: 5260 8030 8123 5260
Size: 2770 93 7656
Fragments arranged by size:
7656 2770 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4918 5819 6748 7483 4918
Size: 901 929 735 7954
Fragments arranged by size:
7954 929 901 735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2240 2259 2433 2544 2721 2748
Size: 399 310 140 19 174 111 177 27
Cuts at: 2748 2855 3042 3150 3402 3482 3552 3596 3647
Size: 107 187 108 252 80 70 44 51
Cuts at: 3647 3802 3876 4032 4184 4326 4778 4869 4896
Size: 155 74 156 152 142 452 91 27
Cuts at: 4896 5603 5667 5689 5723 5816 5974 6002 6229
Size: 707 64 22 34 93 158 28 227
Cuts at: 6229 6249 6365 6664 6883 7076 7158 7253 7813
Size: 20 116 299 219 193 82 95 560
Cuts at: 7813 7838 8580 8665 9736 1391
Size: 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 560 452 399 310
299 252 227 219 193 187 177 174
158 156 155 152 142 140 116 111
108 107 95 93 91 85 82 80
74 70 64 51 44 34 28 27
27 25 22 20 19
BspEI T'CCGG_A
Cuts at: 3341 10182 3341
Size: 6841 3678
BspHI T'CATG_A
Cuts at: 8408 10138 8408
Size: 1730 8789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2871 3850 3966 4103 4533 5322 5538 5709
Size: 2027 979 116 137 430 789 216 171
Cuts at: 5709 6238 7302 7551 7874 844
Size: 529 1064 249 323 3489
Fragments arranged by size:
3489 2027 1064 979 789 529 430 323
249 216 171 137 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 3970 4988 5095 6646 7485 7705
Size: 429 224 1923 1018 107 1551 839 220
Cuts at: 7705 8406 1394
Size: 701 3507
Fragments arranged by size:
3507 1923 1551 1018 839 701 429 224
220 107
BsrDI GCAATG_nn'
Cuts at: 65 1248 3739 3948 9014 9188 65
Size: 1183 2491 209 5066 174 1396
Fragments arranged by size:
5066 2491 1396 1183 209 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2641 3204 3525 3807 3857 3880 5771
Size: 110 831 563 321 282 50 23 1891
Cuts at: 5771 6390 6594 6821 7410 9163 9350 1700
Size: 619 204 227 589 1753 187 2869
Fragments arranged by size:
2869 1891 1753 831 619 589 563 321
282 227 204 187 110 50 23
BsrGI T'GTAC_A
Cuts at: 96 2130 96
Size: 2034 8485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10519
BssSI C'ACGA_G
Cuts at: 2930 5809 6482 6637 8579 9591 2930
Size: 2879 673 155 1942 1012 3858
Fragments arranged by size:
3858 2879 1942 1012 673 155
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2680 794
Size: 1886 8633
BstBI TT'CG_AA
Cuts at: 9323 9323
Size: 10519
BstEII G'GTnAC_C
Cuts at: 1184 5086 1184
Size: 3902 6617
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 3230 4084 4168 4925 5020 5653 7033 2248
Size: 982 854 84 757 95 633 1380 5734
Fragments arranged by size:
5734 1380 982 854 757 633 95 84
BstYI r'GATC_y
Cuts at: 1972 3618 3690 4546 6453 7849 8216 8601 8618
Size: 1646 72 856 1907 1396 367 385 17
Cuts at: 8618 10070 10156 10168 10514 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1907 1646 1452 1396 856 385 367
346 86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 4389 4600 4749 5922 4389
Size: 211 149 1173 8986
Fragments arranged by size:
8986 1173 211 149
BtgI C'CryG_G
Cuts at: 513 1065 2024 3105 4992 5409 5760 6834 7042
Size: 552 959 1081 1887 417 351 1074 208
Cuts at: 7042 7659 7716 513
Size: 617 57 3316
Fragments arranged by size:
3316 1887 1081 1074 959 617 552 417
351 208 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3899 6075 7608 487
Size: 683 56 2673 2176 1533 3398
Fragments arranged by size:
3398 2673 2176 1533 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 2622 3036 3130 4060 5145 5201 8035 8829
Size: 341 414 94 930 1085 56 2834 794
Cuts at: 8829 8849 2281
Size: 20 3951
Fragments arranged by size:
3951 2834 1085 930 794 414 341 94
56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3108 3143 6700 6735 529
Size: 35 2544 35 3557 35 4313
Fragments arranged by size:
4313 3557 2544 35 35 35
DraI TTT'AAA
Cuts at: 7823 7823
Size: 10519
DraIII CAC_nnn'GTG
Cuts at: 4248 6487 4248
Size: 2239 8280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9526 809
Size: 507 8210 1802
Fragments arranged by size:
8210 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2250 2571
Size: 54 1425 50 275 18 191 229 321
Cuts at: 2571 3768 3813 3831 4650 4927 4989 5773 5853
Size: 1197 45 18 819 277 62 784 80
Cuts at: 5853 5911 6197 6325 6819 7330 7557 7713 7966
Size: 58 286 128 494 511 227 156 253
Cuts at: 7966 8855 10484 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1197 889 819 784 511 494
321 286 277 275 253 229 227 191
156 128 80 62 58 54 50 45
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4989 6197 7966 1537
Size: 275 3177 1208 1769 4090
Fragments arranged by size:
4090 3177 1769 1208 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 3101 4910 6045 6791 7280 8449 1353
Size: 1374 374 1809 1135 746 489 1169 3423
Fragments arranged by size:
3423 1809 1374 1169 1135 746 489 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2690 3544 3797 4825 5476 7664
Size: 1064 138 1101 854 253 1028 651 2188
Cuts at: 7664 9106 9480 9626 387
Size: 1442 374 146 1280
Fragments arranged by size:
2188 1442 1280 1101 1064 1028 854 651
374 253 146 138
EcoICRI GAG'CTC
Cuts at: 2719 3148 6363 2719
Size: 429 3215 6875
Fragments arranged by size:
6875 3215 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2234 2291 2474 2483 2618 2724
Size: 393 581 21 57 183 9 135 106
Cuts at: 2724 2870 2879 3305 3584 3593 3837 4037 4301
Size: 146 9 426 279 9 244 200 264
Cuts at: 4301 4401 4823 4877 4891 5413 5690 5889 6557
Size: 100 422 54 14 522 277 199 668
Cuts at: 6557 6602 6979 7145 8582 9179 9966 10234 1239
Size: 45 377 166 1437 597 787 268 1524
Fragments arranged by size:
1524 1437 787 668 597 581 522 426
422 393 377 279 277 268 264 244
200 199 183 166 146 135 106 100
57 54 45 21 14 9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4205 4342 5921 7547 4205
Size: 137 1579 1626 7177
Fragments arranged by size:
7177 1626 1579 137
EcoO109I rG'GnC_Cy
Cuts at: 2623 2935 3001 3399 3447 3798 3799 3973 4753
Size: 312 66 398 48 351 1 174 780
Cuts at: 4753 5129 5970 5971 5998 5999 6353 6512 6943
Size: 376 841 1 27 1 354 159 431
Cuts at: 6943 7021 7250 7291 2623
Size: 78 229 41 5851
Fragments arranged by size:
5851 841 780 431 398 376 354 351
312 229 174 159 78 66 48 41
27 1 1 1
EcoRI G'AATT_C
Cuts at: 7828 8206 7828
Size: 378 10141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3310 4657 6490 6820 3310
Size: 1347 1833 330 7009
Fragments arranged by size:
7009 1833 1347 330
FspI TGC'GCA
Cuts at: 3185 5156 7520 7751 9025 3185
Size: 1971 2364 231 1274 4679
Fragments arranged by size:
4679 2364 1971 1274 231
FspAI rTGC'GCAy
Cuts at: 5156 5156
Size: 10519
HaeII r_GCGC'y
Cuts at: 1678 2342 2639 2913 3727 6692 7504 7659 7801
Size: 664 297 274 814 2965 812 155 142
Cuts at: 7801 9666 1678
Size: 1865 2531
Fragments arranged by size:
2965 2531 1865 814 812 664 297 274
155 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3158 3182 3190 3214 3436
Size: 32 135 32 1586 24 8 24 222
Cuts at: 3436 3468 3502 3534 4667 4699 5407 5439 5529
Size: 32 34 32 1133 32 708 32 90
Cuts at: 5529 5561 7445 7477 7487 7519 7572 7604 7607
Size: 32 1884 32 10 32 53 32 3
Cuts at: 7607 7639 7712 7744 9160 9192 9234 9266 1373
Size: 32 73 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1884 1586 1416 1133 708 222 135
90 73 53 42 34 32 32 32
32 32 32 32 32 32 32 32
32 32 32 24 24 10 8 3
HincII GTy'rAC
Cuts at: 669 1657 1938 5563 5698 7863 8109 669
Size: 988 281 3625 135 2165 246 3079
Fragments arranged by size:
3625 3079 2165 988 281 246 135
HindIII A'AGCT_T
Cuts at: 748 6472 748
Size: 5724 4795
HpaI GTT'AAC
Cuts at: 8109 8109
Size: 10519
KasI G'GCGC_C
Cuts at: 2635 7655 2635
Size: 5020 5499
KpnI G_GTAC'C
Cuts at: 4902 7844 4902
Size: 2942 7577
MfeI C'AATT_G
Cuts at: 8118 8118
Size: 10519
MluI A'CGCG_T
Cuts at: 10395 10395
Size: 10519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 4125 5253 5526 5670 9633 9817 1305
Size: 1506 1314 1128 273 144 3963 184 2007
Fragments arranged by size:
3963 2007 1506 1314 1128 273 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2023 3770 3833 4652 4929
Size: 54 1425 343 191 1747 63 819 277
Cuts at: 4929 5913 7559 7715 10
Size: 984 1646 156 2814
Fragments arranged by size:
2814 1747 1646 1425 984 819 343 277
191 156 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2528 3485 3545 5150 5178 5441
Size: 648 1080 282 957 60 1605 28 263
Cuts at: 5441 5670 6173 6257 6294 6639 8477 8836 8995
Size: 229 503 84 37 345 1838 359 159
Cuts at: 8995 518
Size: 2042
Fragments arranged by size:
2042 1838 1605 1080 957 648 503 359
345 282 263 229 159 84 60 37
28
MspA1I CmG'CkG
Cuts at: 1606 2212 2395 2689 2766 2848 3016 3230 3493
Size: 606 183 294 77 82 168 214 263
Cuts at: 3493 3562 4434 4742 5008 5053 5411 5670 6154
Size: 69 872 308 266 45 358 259 484
Cuts at: 6154 6375 6426 6460 6497 6550 6857 7225 7439
Size: 221 51 34 37 53 307 368 214
Cuts at: 7439 7681 7935 8612 9760 10005 1606
Size: 242 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 606 484 368 358
308 307 294 266 263 259 254 245
242 221 214 214 183 168 82 77
69 53 51 45 37 34
NaeI GCC'GGC
Cuts at: 3206 3527 3859 3882 5773 6823 3206
Size: 321 332 23 1891 1050 6902
Fragments arranged by size:
6902 1891 1050 332 321 23
NarI GG'CG_CC
Cuts at: 2636 7656 2636
Size: 5020 5499
NcoI C'CATG_G
Cuts at: 513 1065 2024 3105 7042 513
Size: 552 959 1081 3937 3990
Fragments arranged by size:
3990 3937 1081 959 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10519
NgoMIV G'CCGG_C
Cuts at: 3204 3525 3857 3880 5771 6821 3204
Size: 321 332 23 1891 1050 6902
Fragments arranged by size:
6902 1891 1050 332 321 23
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10519
NotI GC'GGCC_GC
Cuts at: 7966 7966
Size: 10519
NsiI A_TGCA'T
Cuts at: 2378 2378
Size: 10519
NspI r_CATG'y
Cuts at: 1132 1229 5140 6676 6698 7750 7877 9422 1132
Size: 97 3911 1536 22 1052 127 1545 2229
Fragments arranged by size:
3911 2229 1545 1536 1052 127 97 22
PasI CC'CwG_GG
Cuts at: 1348 2259 3978 6842 1348
Size: 911 1719 2864 5025
Fragments arranged by size:
5025 2864 1719 911
PciI A'CATG_T
Cuts at: 5136 9418 5136
Size: 4282 6237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4213 4940 5236 5272 6535 6953 1108
Size: 3105 727 296 36 1263 418 4674
Fragments arranged by size:
4674 3105 1263 727 418 296 36
PfoI T'CCnGG_A
Cuts at: 2517 3255 5865 7287 2517
Size: 738 2610 1422 5749
Fragments arranged by size:
5749 2610 1422 738
PmeI GTTT'AAAC
Cuts at: 7823 7823
Size: 10519
PmlI CAC'GTG
Cuts at: 5662 5662
Size: 10519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 8588 8620 10101 10133 4019
Size: 32 4537 32 1481 32 4405
Fragments arranged by size:
4537 4405 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2623 3001 3973 5129 6353 7021 7291 2623
Size: 378 972 1156 1224 668 270 5851
Fragments arranged by size:
5851 1224 1156 972 668 378 270
PshAI GACnn'nnGTC
Cuts at: 1613 6550 1613
Size: 4937 5582
PsiI TTA'TAA
Cuts at: 8089 8089
Size: 10519
PspOMI G'GGCC_C
Cuts at: 3398 3798 5970 5998 7249 3398
Size: 400 2172 28 1251 6668
Fragments arranged by size:
6668 2172 1251 400 28
PspXI vC'TCGA_Gb
Cuts at: 6876 6876
Size: 10519
PstI C_TGCA'G
Cuts at: 830 2394 2688 3021 4439 4546 7206 7353 7494
Size: 1564 294 333 1418 107 2660 147 141
Cuts at: 7494 7871 830
Size: 377 3478
Fragments arranged by size:
3478 2660 1564 1418 377 333 294 147
141 107
PvuI CG_AT'CG
Cuts at: 2001 6204 2001
Size: 4203 6316
PvuII CAG'CTG
Cuts at: 2395 2689 2766 2848 3016 3230 3493 3562 5053
Size: 294 77 82 168 214 263 69 1491
Cuts at: 5053 5670 6154 6460 7225 7439 7681 2395
Size: 617 484 306 765 214 242 5233
Fragments arranged by size:
5233 1491 765 617 484 306 294 263
242 214 214 168 82 77 69
SacI G_AGCT'C
Cuts at: 2721 3150 6365 2721
Size: 429 3215 6875
Fragments arranged by size:
6875 3215 429
SacII CC_GC'GG
Cuts at: 5412 5412
Size: 10519
SalI G'TCGA_C
Cuts at: 1936 7861 1936
Size: 5925 4594
SanDI GG'GwC_CC
Cuts at: 2623 3973 5129 2623
Size: 1350 1156 8013
Fragments arranged by size:
8013 1350 1156
SapI GCTCTTCn'nnn_
Cuts at: 2727 7280 2727
Size: 4553 5966
SbfI CC_TGCA'GG
Cuts at: 7494 7871 7494
Size: 377 10142
ScaI AGT'ACT
Cuts at: 2603 4196 4922 8767 10479 2603
Size: 1593 726 3845 1712 2643
Fragments arranged by size:
3845 2643 1712 1593 726
SexAI A'CCwGG_T
Cuts at: 7065 7065
Size: 10519
SfcI C'TryA_G
Cuts at: 826 1045 2390 2684 3017 3457 4435 4542 5371
Size: 219 1345 294 333 440 978 107 829
Cuts at: 5371 7202 7349 7490 7867 9002 9683 9874 826
Size: 1831 147 141 377 1135 681 191 1471
Fragments arranged by size:
1831 1471 1345 1135 978 829 681 440
377 333 294 219 191 147 141 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 5115 7721 5115
Size: 2606 7913
SfoI GGC'GCC
Cuts at: 2637 7657 2637
Size: 5020 5499
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10519
SgrAI Cr'CCGG_yG
Cuts at: 1700 2641 1700
Size: 941 9578
SmaI CCC'GGG
Cuts at: 1872 3590 3923 7846 1872
Size: 1718 333 3923 4545
Fragments arranged by size:
4545 3923 1718 333
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6794 6876 7790 8623 9524 9786
Size: 197 926 4851 82 914 833 901 262
Cuts at: 9786 820
Size: 1553
Fragments arranged by size:
4851 1553 926 914 901 833 262 197
82
SnaBI TAC'GTA
Cuts at: 493 5713 8310 493
Size: 5220 2597 2702
Fragments arranged by size:
5220 2702 2597
SpeI A'CTAG_T
Cuts at: 152 6809 152
Size: 6657 3862
SphI G_CATG'C
Cuts at: 1132 7750 7877 1132
Size: 6618 127 3774
Fragments arranged by size:
6618 3774 127
SspI AAT'ATT
Cuts at: 5 52 8443 5
Size: 47 8391 2081
Fragments arranged by size:
8391 2081 47
StuI AGG'CCT
Cuts at: 1517 2813 3653 7565 1517
Size: 1296 840 3912 4471
Fragments arranged by size:
4471 3912 1296 840
StyI C'CwwG_G
Cuts at: 513 1065 2024 3105 3294 3390 4833 7042 7279
Size: 552 959 1081 189 96 1443 2209 237
Cuts at: 7279 7560 7958 513
Size: 281 398 3074
Fragments arranged by size:
3074 2209 1443 1081 959 552 398 281
237 189 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3240 4094 5105 6707 7151 8546 8563
Size: 1255 1660 854 1011 1602 444 1395 17
Cuts at: 8563 8716 8901 9346 325
Size: 153 185 445 1498
Fragments arranged by size:
1660 1602 1498 1395 1255 1011 854 445
444 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2601 3609
Size: 274 80 33 51 1443 153 471 1008
Cuts at: 3609 4194 4920 7317 8765 10477 96
Size: 585 726 2397 1448 1712 138
Fragments arranged by size:
2397 1712 1448 1443 1008 726 585 471
274 153 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 5126 5158 5339 5370 5371 5402 6950
Size: 32 1528 32 181 31 1 31 1548
Cuts at: 6950 6982 7283 7315 10479 10511 3566
Size: 32 301 32 3164 32 3574
Fragments arranged by size:
3574 3164 1548 1528 301 181 32 32
32 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 2525 1316
Size: 1209 9310
XbaI T'CTAG_A
Cuts at: 7855 7855
Size: 10519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2833 3253 4937 5906 6256 6532 6712 6895 7615
Size: 420 1684 969 350 276 180 183 720
Cuts at: 7615 2833
Size: 5737
Fragments arranged by size:
5737 1684 969 720 420 350 276 183
180
XhoI C'TCGA_G
Cuts at: 1943 6876 1943
Size: 4933 5586
XmaI C'CCGG_G
Cuts at: 1870 3588 3921 7844 1870
Size: 1718 333 3923 4545
Fragments arranged by size:
4545 3923 1718 333
XmnI GAAnn'nnTTC
Cuts at: 5189 8648 5189
Size: 3459 7060
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3327 8327 276
Size: 53 83 186 936 1793 5000 2468
Fragments arranged by size:
5000 2468 1793 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I EcoRV FseI NruI
PacI PsrI RsrII SrfI SwaI