Restriction map for
pFN21AA0397
(Circular) (Six-base) MAPSORT of: pFN21AA0397.seq Check: 6010 from: 1 to: 7723
pFN21AA0397 7723 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3090 3775 3808 3090
Size: 685 33 7005
Fragments arranged by size:
7005 685 33
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3729 4470 5533 278
Size: 53 83 186 936 2193 741 1063 2468
Fragments arranged by size:
2468 2193 1063 936 741 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5066 1937
Size: 3129 4594
Acc65I G'GTAC_C
Cuts at: 3810 5044 3810
Size: 1234 6489
AclI AA'CG_TT
Cuts at: 1563 5850 6223 1563
Size: 4287 373 3063
Fragments arranged by size:
4287 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 2953 4038 4567 5786 7170 7438 1632
Size: 1265 56 1085 529 1219 1384 268 1917
Fragments arranged by size:
1917 1384 1265 1219 1085 529 268 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7526
AflIII A'CryG_T
Cuts at: 1203 2389 3627 4482 6622 7599 1203
Size: 1186 1238 855 2140 977 1327
Fragments arranged by size:
2140 1327 1238 1186 977 855
AgeI A'CCGG_T
Cuts at: 3444 6554 3444
Size: 3110 4613
AhdI GACnn_n'nnGTC
Cuts at: 6452 6452
Size: 7723
AleI CACnn'nnGTG
Cuts at: 1166 2225 3020 4073 1166
Size: 1059 795 1053 4816
Fragments arranged by size:
4816 1059 1053 795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 3038 4971 1184
Size: 1690 164 1933 3936
Fragments arranged by size:
3936 1933 1690 164
AlwNI CAG_nnn'CTG
Cuts at: 2278 2498 3426 4675 5466 7038 2278
Size: 220 928 1249 791 1572 2963
Fragments arranged by size:
2963 1572 1249 928 791 220
ApaI G_GGCC'C
Cuts at: 3330 4239 3330
Size: 909 6814
ApaLI G'TGCA_C
Cuts at: 3566 5780 6936 3566
Size: 2214 1156 4353
Fragments arranged by size:
4353 2214 1156
ApoI r'AATT_y
Cuts at: 1493 5032 5258 5410 7519 1493
Size: 3539 226 152 2109 1697
Fragments arranged by size:
3539 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6277 160
Size: 872 5245 1606
Fragments arranged by size:
5245 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 3495 3598 4055 4153 4451 4980
Size: 73 1180 372 103 457 98 298 529
Cuts at: 4980 5048 1870
Size: 68 4545
Fragments arranged by size:
4545 1180 529 457 372 298 103 98
73 68
BamHI G'GATC_C
Cuts at: 2676 4273 5053 5420 2676
Size: 1597 780 367 4979
Fragments arranged by size:
4979 1597 780 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2616 2962 3345 3810 4067
Size: 325 217 627 829 346 383 465 257
Cuts at: 4067 4534 4628 5044 6499 618
Size: 467 94 416 1455 1842
Fragments arranged by size:
1842 1455 829 627 467 465 416 383
346 325 257 217 94
BanII G_rGCy'C
Cuts at: 1391 2345 3141 3330 3357 3374 4008 4239 4269
Size: 954 796 189 27 17 634 231 30
Cuts at: 4269 5042 1391
Size: 773 4072
Fragments arranged by size:
4072 954 796 773 634 231 189 30
27 17
BbeI G_GCGC'C
Cuts at: 4538 4538
Size: 7723
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 2240 928
Size: 971 216 125 6411
Fragments arranged by size:
6411 971 216 125
BbvCI CC'TCA_GC
Cuts at: 2369 2369
Size: 7723
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 5914 5948 1903
Size: 34 1961 34 1982 34 3678
Fragments arranged by size:
3678 1982 1961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5615 6825 5615
Size: 1210 6513
BclI T'GATC_A
Cuts at: 1548 4561 1548
Size: 3013 4710
BfrBI ATG'CAT
Cuts at: 4702 4702
Size: 7723
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3039 3343 3457
Size: 107 122 71 631 605 1367 304 114
Cuts at: 3457 6334 136
Size: 2877 1525
Fragments arranged by size:
2877 1525 1367 631 605 304 122 114
107 71
BglII A'GATC_T
Cuts at: 7718 7718
Size: 7723
BlpI GC'TnA_GC
Cuts at: 2344 4944 5140 2344
Size: 2600 196 4927
Fragments arranged by size:
4927 2600 196
Bme1580I G_kGCm'C
Cuts at: 1790 2243 2734 2967 3233 3330 3570 4070 4239
Size: 453 491 233 266 97 240 500 169
Cuts at: 4239 5784 6940 1790
Size: 1545 1156 2573
Fragments arranged by size:
2573 1545 1156 500 491 453 266 240
233 169 97
BmgBI CAC'GTC
Cuts at: 1318 2238 3628 4501 1318
Size: 920 1390 873 4540
Fragments arranged by size:
4540 1390 920 873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2237 2953 5454
Size: 416 53 83 399 24 820 716 2501
Cuts at: 5454 6412 442
Size: 958 1753
Fragments arranged by size:
2501 1753 958 820 716 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2889 4123 4243 2889
Size: 1234 120 6369
Fragments arranged by size:
6369 1234 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3125 3371 3379 3692 4095 4213 4328 6383
Size: 1886 246 8 313 403 118 115 2055
Cuts at: 6383 1239
Size: 2579
Fragments arranged by size:
2579 2055 1886 403 313 246 118 115
8
Bpu10I CC'TnA_GC
Cuts at: 2369 3455 4038 2369
Size: 1086 583 6054
Fragments arranged by size:
6054 1086 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2677 5848 6713 7011 2546
Size: 131 3171 865 298 3258
Fragments arranged by size:
3258 3171 865 298 131
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6386 882
Size: 617 4887 2219
Fragments arranged by size:
4887 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2797 3928 4483 5514 493
Size: 711 1188 405 1131 555 1031 2702
Fragments arranged by size:
2702 1188 1131 1031 711 555 405
BsaBI GATnn'nnATC
Cuts at: 7717 7717
Size: 7723
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2173 3726 4467 4535
Size: 53 83 186 936 640 1553 741 68
Cuts at: 4535 5530 5912 6547 275
Size: 995 382 635 1451
Fragments arranged by size:
1553 1451 995 936 741 640 635 382
186 83 68 53
BsaWI w'CCGG_w
Cuts at: 2679 3258 3444 6156 6554 6828 6975 7386 2679
Size: 579 186 2712 398 274 147 411 3016
Fragments arranged by size:
3016 2712 579 411 398 274 186 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3344 3374 3935 3965 4643
Size: 30 165 30 1941 30 561 30 678
Cuts at: 4643 4673 1178
Size: 30 4228
Fragments arranged by size:
4228 1941 678 561 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 3236 3364 3382 3892 4120 4217 4334
Size: 907 1143 128 18 510 228 97 117
Cuts at: 4334 1186
Size: 4575
Fragments arranged by size:
4575 1143 907 510 228 128 117 97
18
BseYI C'CCAG_C
Cuts at: 2665 3153 3329 3419 3880 4277 6926 7422 2665
Size: 488 176 90 461 397 2649 496 2966
Fragments arranged by size:
2966 2649 496 488 461 397 176 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 2738 2825 2834 3382 3950 4370
Size: 573 424 578 87 9 548 568 420
Cuts at: 4370 4427 4495 4532 5021 1163
Size: 57 68 37 489 3865
Fragments arranged by size:
3865 578 573 568 548 489 424 420
87 68 57 37 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 5173 5934 6962 1540
Size: 275 186 1444 1728 761 1028 2301
Fragments arranged by size:
2301 1728 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 3570 3711 4494 4839 5784 5869 6940 3291
Size: 279 141 783 345 945 85 1071 4074
Fragments arranged by size:
4074 1071 945 783 345 279 141 85
BsmI GAATG_Cn'
Cuts at: 3201 4090 5234 5327 3201
Size: 889 1144 93 5597
Fragments arranged by size:
5597 1144 889 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2164 4458 2164
Size: 2294 5429
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2345 2734 2967 3141 3233 3291
Size: 399 453 102 389 233 174 92 58
Cuts at: 3291 3330 3357 3374 3570 3711 4008 4070 4239
Size: 39 27 17 196 141 297 62 169
Cuts at: 4239 4269 4494 4839 5042 5784 5869 6940 1391
Size: 30 225 345 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 389 345 297
233 225 203 196 174 169 141 102
92 85 62 58 39 30 27 17
BspEI T'CCGG_A
Cuts at: 2679 3258 7386 2679
Size: 579 4128 3016
Fragments arranged by size:
4128 3016 579
BspHI T'CATG_A
Cuts at: 2898 4593 5612 7342 2898
Size: 1695 1019 1730 3279
Fragments arranged by size:
3279 1730 1695 1019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3090 3775 3808 4392 5078 844
Size: 2246 685 33 584 686 3489
Fragments arranged by size:
3489 2246 686 685 584 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3009 3454 3868 5610 1394
Size: 429 1186 445 414 1742 3507
Fragments arranged by size:
3507 1742 1186 445 429 414
BsrDI GCAATG_nn'
Cuts at: 65 1248 2124 6218 6392 65
Size: 1183 876 4094 174 1396
Fragments arranged by size:
4094 1396 1183 876 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3444 3591 6367 6554 1700
Size: 110 1634 147 2776 187 2869
Fragments arranged by size:
2869 2776 1634 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7723
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7723
BssSI C'ACGA_G
Cuts at: 2957 3284 3710 5783 6795 2957
Size: 327 426 2073 1012 3885
Fragments arranged by size:
3885 2073 1012 426 327
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2991 794
Size: 2197 5526
BstBI TT'CG_AA
Cuts at: 4197 6527 4197
Size: 2330 5393
BstEII G'GTnAC_C
Cuts at: 1184 4546 1184
Size: 3362 4361
BstYI r'GATC_y
Cuts at: 1972 2676 3714 4273 4671 4965 5053 5420 5805
Size: 704 1038 559 398 294 88 367 385
Cuts at: 5805 5822 7274 7360 7372 7718 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1038 704 559 398 385 367
346 294 88 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2308 2376 3904 4293 2308
Size: 68 1528 389 5738
Fragments arranged by size:
5738 1528 389 68
BtgI C'CryG_G
Cuts at: 513 1065 2004 3210 3537 3609 4074 4095 4146
Size: 552 939 1206 327 72 465 21 51
Cuts at: 4146 4632 513
Size: 486 3604
Fragments arranged by size:
3604 1206 939 552 486 465 327 72
51 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4837 487
Size: 683 56 3611 3373
Fragments arranged by size:
3611 3373 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2203 2483 2989 3520 3801 5239 6033 6053
Size: 55 280 506 531 281 1438 794 20
Cuts at: 6053 2148
Size: 3818
Fragments arranged by size:
3818 1438 794 531 506 281 280 55
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7688
DraI TTT'AAA
Cuts at: 5027 5027
Size: 7723
DraIII CAC_nnn'GTG
Cuts at: 4999 4999
Size: 7723
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3553 6730 809
Size: 507 2237 3177 1802
Fragments arranged by size:
3177 2237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3274 3745 5170
Size: 54 1425 50 275 18 1444 471 1425
Cuts at: 5170 6059 7688 8
Size: 889 1629 43
Fragments arranged by size:
1629 1444 1425 1425 889 471 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5170 1537
Size: 275 3358 4090
Fragments arranged by size:
4090 3358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 3473 4194 4592 5653 1353
Size: 1176 944 721 398 1061 3423
Fragments arranged by size:
3423 1176 1061 944 721 398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 6310 6684 6830 387
Size: 1064 138 876 3845 374 146 1280
Fragments arranged by size:
3845 1280 1064 876 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2897 2953 3125 3371 3379 3692 4038
Size: 393 1265 56 172 246 8 313 346
Cuts at: 4038 4095 4213 4328 4567 5786 6383 7170 7438
Size: 57 118 115 239 1219 597 787 268
Cuts at: 7438 1239
Size: 1524
Fragments arranged by size:
1524 1265 1219 787 597 393 346 313
268 246 239 172 118 115 57 56
8
EcoNI CCTnn'n_nnAGG
Cuts at: 3620 4175 4868 3620
Size: 555 693 6475
Fragments arranged by size:
6475 693 555
EcoO109I rG'GnC_Cy
Cuts at: 2326 2359 2534 3603 4012 4052 4984 2326
Size: 33 175 1069 409 40 932 5065
Fragments arranged by size:
5065 1069 932 409 175 40 33
EcoRI G'AATT_C
Cuts at: 5032 5410 5032
Size: 378 7345
FspI TGC'GCA
Cuts at: 2195 6229 2195
Size: 4034 3689
HaeII r_GCGC'y
Cuts at: 1678 2411 2665 3563 3937 4538 6870 1678
Size: 733 254 898 374 601 2332 2531
Fragments arranged by size:
2531 2332 898 733 601 374 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3625 3657 4872
Size: 32 135 32 985 32 1036 32 1215
Cuts at: 4872 4904 6364 6396 6438 6470 1373
Size: 32 1460 32 42 32 2626
Fragments arranged by size:
2626 1460 1215 1036 985 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5067 5313 669
Size: 988 281 3129 246 3079
Fragments arranged by size:
3129 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7723
HpaI GTT'AAC
Cuts at: 5313 5313
Size: 7723
KasI G'GCGC_C
Cuts at: 4534 4534
Size: 7723
KpnI G_GTAC'C
Cuts at: 3814 5048 3814
Size: 1234 6489
MfeI C'AATT_G
Cuts at: 5322 5322
Size: 7723
MluI A'CGCG_T
Cuts at: 7599 7599
Size: 7723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 6837 7021 1305
Size: 1383 4149 184 2007
Fragments arranged by size:
4149 2007 1383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3747 10
Size: 54 1425 343 1915 3986
Fragments arranged by size:
3986 1915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 3020 4073 4707 4953 5681 6040
Size: 648 1059 795 1053 634 246 728 359
Cuts at: 6040 6199 518
Size: 159 2042
Fragments arranged by size:
2042 1059 1053 795 728 648 634 359
246 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2183 2352 2614 3058 3145 3611 3862
Size: 561 16 169 262 444 87 466 251
Cuts at: 3862 4567 4578 5139 5816 6964 7209 1606
Size: 705 11 561 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 561 561 466 444
262 251 245 169 87 16 11
NarI GG'CG_CC
Cuts at: 4535 4535
Size: 7723
NcoI C'CATG_G
Cuts at: 513 1065 2004 4632 513
Size: 552 939 2628 3604
Fragments arranged by size:
3604 2628 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7723
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7723
NotI GC'GGCC_GC
Cuts at: 5170 5170
Size: 7723
NsiI A_TGCA'T
Cuts at: 4704 4704
Size: 7723
NspI r_CATG'y
Cuts at: 1132 1229 3413 4522 4657 5081 6626 1132
Size: 97 2184 1109 135 424 1545 2229
Fragments arranged by size:
2229 2184 1545 1109 424 135 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 6400
PciI A'CATG_T
Cuts at: 6622 6622
Size: 7723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2800 4675 1108
Size: 1692 1875 4156
Fragments arranged by size:
4156 1875 1692
PfoI T'CCnGG_A
Cuts at: 4674 4674
Size: 7723
PmeI GTTT'AAAC
Cuts at: 5027 5027
Size: 7723
PmlI CAC'GTG
Cuts at: 2797 3928 2797
Size: 1131 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5792 5824 7305 7337 5792
Size: 32 1481 32 6178
Fragments arranged by size:
6178 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2534 4012 4052 4984 2534
Size: 1478 40 932 5273
Fragments arranged by size:
5273 1478 932 40
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7723
PsiI TTA'TAA
Cuts at: 5293 5293
Size: 7723
PspOMI G'GGCC_C
Cuts at: 3326 4235 3326
Size: 909 6814
PstI C_TGCA'G
Cuts at: 830 2283 2462 2863 4249 5075 830
Size: 1453 179 401 1386 826 3478
Fragments arranged by size:
3478 1453 1386 826 401 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7723
PvuII CAG'CTG
Cuts at: 2167 4567 4578 2167
Size: 2400 11 5312
Fragments arranged by size:
5312 2400 11
SacII CC_GC'GG
Cuts at: 3612 3612
Size: 7723
SalI G'TCGA_C
Cuts at: 1936 5065 1936
Size: 3129 4594
SanDI GG'GwC_CC
Cuts at: 2534 2534
Size: 7723
SbfI CC_TGCA'GG
Cuts at: 5075 5075
Size: 7723
ScaI AGT'ACT
Cuts at: 2468 5971 7683 2468
Size: 3503 1712 2508
Fragments arranged by size:
3503 2508 1712
SfcI C'TryA_G
Cuts at: 826 1045 2279 2458 2859 3427 4245 4353 4573
Size: 219 1234 179 401 568 818 108 220
Cuts at: 4573 5071 6206 6887 7078 826
Size: 498 1135 681 191 1471
Fragments arranged by size:
1471 1234 1135 818 681 568 498 401
220 219 191 179 108
SfoI GGC'GCC
Cuts at: 4536 4536
Size: 7723
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7723
SmaI CCC'GGG
Cuts at: 1872 3497 3600 4057 4982 5050 1872
Size: 1625 103 457 925 68 4545
Fragments arranged by size:
4545 1625 925 457 103 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 2692 4451 5827 6728 6990
Size: 197 926 618 131 1759 1376 901 262
Cuts at: 6990 820
Size: 1553
Fragments arranged by size:
1759 1553 1376 926 901 618 262 197
131
SnaBI TAC'GTA
Cuts at: 493 5514 493
Size: 5021 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7723
SphI G_CATG'C
Cuts at: 1132 5081 1132
Size: 3949 3774
SspI AAT'ATT
Cuts at: 5 52 5647 5
Size: 47 5595 2081
Fragments arranged by size:
5595 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 3839 4874 1517
Size: 864 1458 1035 4366
Fragments arranged by size:
4366 1458 1035 864
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 2544 3621 4632 5162 513
Size: 552 939 219 321 1077 1011 530 3074
Fragments arranged by size:
3074 1077 1011 939 552 530 321 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3894 4655 4736 5750 5767 5920 6105
Size: 1255 2314 761 81 1014 17 153 185
Cuts at: 6105 6550 325
Size: 445 1498
Fragments arranged by size:
2314 1498 1255 1014 761 445 185 153
81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 5969 7681
Size: 274 80 33 51 1443 489 3503 1712
Cuts at: 7681 96
Size: 138
Fragments arranged by size:
3503 1712 1443 489 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3071 3103 3344 3376 3935 3967 7683 7715 3071
Size: 32 241 32 559 32 3716 32 3079
Fragments arranged by size:
3716 3079 559 241 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 2432 4988 1316
Size: 708 408 2556 4051
Fragments arranged by size:
4051 2556 708 408
XbaI T'CTAG_A
Cuts at: 5059 5059
Size: 7723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2900 2900
Size: 7723
XhoI C'TCGA_G
Cuts at: 1943 4451 1943
Size: 2508 5215
XmaI C'CCGG_G
Cuts at: 1870 3495 3598 4055 4980 5048 1870
Size: 1625 103 457 925 68 4545
Fragments arranged by size:
4545 1625 925 457 103 68
XmnI GAAnn'nnTTC
Cuts at: 3173 5852 3173
Size: 2679 5044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3727 4468 5531 276
Size: 53 83 186 936 2193 741 1063 2468
Fragments arranged by size:
2468 2193 1063 936 741 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstXI BstZ17I
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PspXI PsrI RsrII SacI SapI SexAI SfiI
SrfI SwaI