Restriction map for
pFN21AA0395
(Circular) (Six-base) MAPSORT of: pFN21AA0395.seq Check: 8225 from: 1 to: 7573
pFN21AA0395 7573 bp
With 182 enzymes: SgfI *
March 28, 2012 12:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 5383 278
Size: 53 83 186 936 3322 525 2468
Fragments arranged by size:
3322 2468 936 525 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 4916 1937
Size: 723 2256 4594
Fragments arranged by size:
4594 2256 723
Acc65I G'GTAC_C
Cuts at: 4894 4894
Size: 7573
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2535 2607 2981 3095 4211 4230 4481
Size: 495 408 72 374 114 1116 19 251
Cuts at: 4481 5636 7020 7288 1632
Size: 1155 1384 268 1917
Fragments arranged by size:
1917 1384 1155 1116 495 408 374 268
251 114 72 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 2393 6472 7449 1203
Size: 1190 4079 977 1327
Fragments arranged by size:
4079 1327 1190 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 3281 6302 3281
Size: 3021 4552
AleI CACnn'nnGTG
Cuts at: 1166 3590 1166
Size: 2424 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4668 1184
Size: 3484 4089
AlwNI CAG_nnn'CTG
Cuts at: 2125 2872 5316 6888 2125
Size: 747 2444 1572 2810
Fragments arranged by size:
2810 2444 1572 747
ApaI G_GGCC'C
Cuts at: 2772 4715 2772
Size: 1943 5630
ApaLI G'TGCA_C
Cuts at: 5630 6786 5630
Size: 1156 6417
ApoI r'AATT_y
Cuts at: 1493 2598 4882 5108 5260 7369 1493
Size: 1105 2284 226 152 2109 1697
Fragments arranged by size:
2284 2109 1697 1105 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 4898 1870
Size: 73 2955 4545
Fragments arranged by size:
4545 2955 73
BamHI G'GATC_C
Cuts at: 4903 5270 4903
Size: 367 7206
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 3819 4244 4894 6349
Size: 325 217 627 1586 446 425 650 1455
Cuts at: 6349 618
Size: 1842
Fragments arranged by size:
1842 1586 1455 650 627 446 425 325
217
BanII G_rGCy'C
Cuts at: 1391 2508 2772 3006 3668 3865 3883 4551 4631
Size: 1117 264 234 662 197 18 668 80
Cuts at: 4631 4715 4731 4800 4827 4892 1391
Size: 84 16 69 27 65 4072
Fragments arranged by size:
4072 1117 668 662 264 234 197 84
80 69 65 27 18 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 4494 928
Size: 971 158 2437 4007
Fragments arranged by size:
4007 2437 971 158
BbvCI CC'TCA_GC
Cuts at: 3156 4011 4029 4065 3156
Size: 855 18 36 6664
Fragments arranged by size:
6664 855 36 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5764 5798 1903
Size: 34 3827 34 3678
Fragments arranged by size:
3827 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3042 5465 6675 3042
Size: 2423 1210 3940
Fragments arranged by size:
3940 2423 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7573
BfrBI ATG'CAT
Cuts at: 2037 2037
Size: 7573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6184 136
Size: 107 122 71 631 605 4512 1525
Fragments arranged by size:
4512 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3051 7568 3051
Size: 4517 3056
BlpI GC'TnA_GC
Cuts at: 4990 4990
Size: 7573
Bme1580I G_kGCm'C
Cuts at: 1790 2772 4715 5634 6790 1790
Size: 982 1943 919 1156 2573
Fragments arranged by size:
2573 1943 1156 982 919
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 2502 2834
Size: 416 53 83 399 24 622 463 332
Cuts at: 2834 3427 5304 6262 442
Size: 593 1877 958 1753
Fragments arranged by size:
1877 1753 958 622 593 463 416 399
332 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3489 1056
Size: 2433 5140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4834 4057
Size: 777 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 2810 3652 3705 3905 6233 1239
Size: 1446 125 842 53 200 2328 2579
Fragments arranged by size:
2579 2328 1446 842 200 125 53
Bpu10I CC'TnA_GC
Cuts at: 2912 3156 3867 4011 4029 4065 4103 2912
Size: 244 711 144 18 36 38 6382
Fragments arranged by size:
6382 711 244 144 38 36 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 3032 5698 6563 6861 2594
Size: 438 2666 865 298 3306
Fragments arranged by size:
3306 2666 865 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 4010 4114 4667 6236 882
Size: 617 591 1920 104 553 1569 2219
Fragments arranged by size:
2219 1920 1569 617 591 553 104
BsaAI yAC'GTr
Cuts at: 493 1204 5364 493
Size: 711 4160 2702
Fragments arranged by size:
4160 2702 711
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4855 5380 5762 6397
Size: 53 83 186 936 3322 525 382 635
Cuts at: 6397 275
Size: 1451
Fragments arranged by size:
3322 1451 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6006 6404 6678 6825 7236 6006
Size: 398 274 147 411 6343
Fragments arranged by size:
6343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2470 2500 2539 2569 3529
Size: 30 165 30 1067 30 39 30 960
Cuts at: 3529 3559 4373 4403 1178
Size: 30 814 30 4348
Fragments arranged by size:
4348 1067 960 814 165 39 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3116 3431 4028 4046 4070 4685 1186
Size: 1930 315 597 18 24 615 4074
Fragments arranged by size:
4074 1930 615 597 315 24 18
BseYI C'CCAG_C
Cuts at: 2085 2115 2171 3455 4139 4240 4323 4632 6776
Size: 30 56 1284 684 101 83 309 2144
Cuts at: 6776 7272 2085
Size: 496 2386
Fragments arranged by size:
2386 2144 1284 684 496 309 101 83
56 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 1163
Size: 573 629 6371
Fragments arranged by size:
6371 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5023 5784 6812 1540
Size: 275 186 3022 761 1028 2301
Fragments arranged by size:
3022 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2304 2480 3006 4551 4631 4731 5634 5719
Size: 88 176 526 1545 80 100 903 85
Cuts at: 5719 6790 2216
Size: 1071 2999
Fragments arranged by size:
2999 1545 1071 903 526 176 100 88
85 80
BsmI GAATG_Cn'
Cuts at: 2919 3765 4012 4042 5084 5177 2919
Size: 846 247 30 1042 93 5315
Fragments arranged by size:
5315 1042 846 247 93 30
BsmBI CGTCTCn'nnnn_
Cuts at: 3985 3985
Size: 7573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2304 2480 2508 2772 3006 3668
Size: 399 426 88 176 28 264 234 662
Cuts at: 3668 3865 3883 4551 4631 4715 4731 4800 4827
Size: 197 18 668 80 84 16 69 27
Cuts at: 4827 4892 5634 5719 6790 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 662 426 399 264
234 197 176 88 85 84 80 69
65 28 27 18 16
BspEI T'CCGG_A
Cuts at: 7236 7236
Size: 7573
BspHI T'CATG_A
Cuts at: 2562 3511 5462 7192 2562
Size: 949 1951 1730 2943
Fragments arranged by size:
2943 1951 1730 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3770 4440 4605 4627 4928 844
Size: 2926 670 165 22 301 3489
Fragments arranged by size:
3489 2926 670 301 165 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3230 5460 1394
Size: 429 1407 2230 3507
Fragments arranged by size:
3507 2230 1407 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3211 4527 6068 6242 65
Size: 1183 1963 1316 1541 174 1396
Fragments arranged by size:
1963 1541 1396 1316 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6217 6404 1700
Size: 110 4407 187 2869
Fragments arranged by size:
4407 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 5633 6645 5633
Size: 1012 6561
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 2144 2343 4339 794
Size: 1331 19 199 1996 4028
Fragments arranged by size:
4028 1996 1331 199 19
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 3022 3757 1184
Size: 1838 735 5000
Fragments arranged by size:
5000 1838 735
BstXI CCAn_nnnn'nTGG
Cuts at: 4684 4715 4684
Size: 31 7542
BstYI r'GATC_y
Cuts at: 1972 2122 2258 3051 4903 5270 5655 5672 7124
Size: 150 136 793 1852 367 385 17 1452
Cuts at: 7124 7210 7222 7568 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1852 1452 793 385 367 346 150
136 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2367 3099 3753 4199 4257 4809 2367
Size: 732 654 446 58 552 5131
Fragments arranged by size:
5131 732 654 552 446 58
BtgI C'CryG_G
Cuts at: 513 1065 2004 2877 2940 4415 4683 513
Size: 552 939 873 63 1475 268 3403
Fragments arranged by size:
3403 1475 939 873 552 268 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3689 487
Size: 683 56 2463 4371
Fragments arranged by size:
4371 2463 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2344 2870 3646 4652 4705 5089 5883 5903
Size: 186 526 776 1006 53 384 794 20
Cuts at: 5903 2158
Size: 3828
Fragments arranged by size:
3828 1006 794 776 526 384 186 53
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 4877 4877
Size: 7573
DraIII CAC_nnn'GTG
Cuts at: 3247 3390 3247
Size: 143 7430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2196 2430
Size: 54 1425 50 275 18 177 189 234
Cuts at: 2430 2880 3300 4092 4367 4418 4468 5020 5909
Size: 450 420 792 275 51 50 552 889
Cuts at: 5909 7538 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 792 552 450 420 275
275 234 189 177 54 51 50 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5020 1537
Size: 275 3208 4090
Fragments arranged by size:
4090 3208 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3237 3915 4047 4077 4584 5503 1353
Size: 1884 678 132 30 507 919 3423
Fragments arranged by size:
3423 1884 919 678 507 132 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6160 6534 6680 387
Size: 1064 138 4571 374 146 1280
Fragments arranged by size:
4571 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3004 4549 4629 4729 3004
Size: 1545 80 100 5848
Fragments arranged by size:
5848 1545 100 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2535 2607 2685 2810 2981 3095
Size: 393 495 408 72 78 125 171 114
Cuts at: 3095 3652 3705 3905 4211 4230 4481 5636 6233
Size: 557 53 200 306 19 251 1155 597
Cuts at: 6233 7020 7288 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1155 787 597 557 495 408 393
306 268 251 200 171 125 114 78
72 53 19
EcoO109I rG'GnC_Cy
Cuts at: 2111 2769 2887 3748 4594 4711 4712 2111
Size: 658 118 861 846 117 1 4972
Fragments arranged by size:
4972 861 846 658 118 117 1
EcoRI G'AATT_C
Cuts at: 4882 5260 4882
Size: 378 7195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 3494 2986
Size: 508 7065
FspI TGC'GCA
Cuts at: 6079 6079
Size: 7573
HaeII r_GCGC'y
Cuts at: 1678 6720 1678
Size: 5042 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 3016 3048 3267
Size: 32 135 32 671 32 741 32 219
Cuts at: 3267 3299 3529 3561 4253 4285 4373 4405 4845
Size: 32 230 32 692 32 88 32 440
Cuts at: 4845 4877 6214 6246 6288 6320 1373
Size: 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1337 741 692 671 440 230 219
135 88 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 4917 5163 669
Size: 988 281 723 2256 246 3079
Fragments arranged by size:
3079 2256 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KpnI G_GTAC'C
Cuts at: 4898 4898
Size: 7573
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2948 3527 3794 6687 6871 1305
Size: 1643 579 267 2893 184 2007
Fragments arranged by size:
2893 2007 1643 579 267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2198 2432 3302 4094
Size: 54 1425 343 177 189 234 870 792
Cuts at: 4094 4369 10
Size: 275 3214
Fragments arranged by size:
3214 1425 870 792 343 275 234 189
177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 5531 5890 6049 518
Size: 648 2424 1941 359 159 2042
Fragments arranged by size:
2424 2042 1941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2801 3090 3410 3520 4330 4989 5666
Size: 487 708 289 320 110 810 659 677
Cuts at: 5666 6814 7059 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 810 708 677 659 487 320
289 245 110
NcoI C'CATG_G
Cuts at: 513 1065 2004 2940 513
Size: 552 939 936 5146
Fragments arranged by size:
5146 939 936 552
NdeI CA'TA_TG
Cuts at: 387 2289 387
Size: 1902 5671
NheI G'CTAG_C
Cuts at: 1052 3485 1052
Size: 2433 5140
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NsiI A_TGCA'T
Cuts at: 2039 2039
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2397 2849 4931 6476 1132
Size: 97 1168 452 2082 1545 2229
Fragments arranged by size:
2229 2082 1545 1168 452 97
PasI CC'CwG_GG
Cuts at: 1348 3560 1348
Size: 2212 5361
PciI A'CATG_T
Cuts at: 2393 6472 2393
Size: 4079 3494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 2635 4153 1108
Size: 1173 354 1518 4528
Fragments arranged by size:
4528 1518 1173 354
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 3748 4594 2111
Size: 1637 846 5090
Fragments arranged by size:
5090 1637 846
PshAI GACnn'nnGTC
Cuts at: 1613 3350 1613
Size: 1737 5836
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 2768 4711 2768
Size: 1943 5630
PstI C_TGCA'G
Cuts at: 830 4557 4602 4925 830
Size: 3727 45 323 3478
Fragments arranged by size:
3727 3478 323 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 2801 3090 3410 3520 2801
Size: 289 320 110 6854
Fragments arranged by size:
6854 320 289 110
SacI G_AGCT'C
Cuts at: 3006 4551 4631 4731 3006
Size: 1545 80 100 5848
Fragments arranged by size:
5848 1545 100 80
SalI G'TCGA_C
Cuts at: 1936 2659 4915 1936
Size: 723 2256 4594
Fragments arranged by size:
4594 2256 723
SapI GCTCTTCn'nnn_
Cuts at: 3237 3237
Size: 7573
SbfI CC_TGCA'GG
Cuts at: 4557 4925 4557
Size: 368 7205
ScaI AGT'ACT
Cuts at: 2179 5821 7533 2179
Size: 3642 1712 2219
Fragments arranged by size:
3642 2219 1712
SfcI C'TryA_G
Cuts at: 826 1045 4553 4598 4921 6056 6737 6928 826
Size: 219 3508 45 323 1135 681 191 1471
Fragments arranged by size:
3508 1471 1135 681 323 219 191 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 4900 1872
Size: 3028 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 3047 5677 6578 6840 820
Size: 197 926 666 438 2630 901 262 1553
Fragments arranged by size:
2630 1553 926 901 666 438 262 197
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 2849 4931 1132
Size: 1717 2082 3774
Fragments arranged by size:
3774 2082 1717
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 1517
Size: 1128 6445
StyI C'CwwG_G
Cuts at: 513 1065 2004 2883 2940 3744 5012 513
Size: 552 939 879 57 804 1268 3074
Fragments arranged by size:
3074 1268 939 879 804 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2331 2627 3391 4232 4742 5600 5617
Size: 1255 751 296 764 841 510 858 17
Cuts at: 5617 5770 5955 6400 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 858 841 764 751 510 445
296 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2177 5819 7531
Size: 274 80 33 51 1443 200 3642 1712
Cuts at: 7531 96
Size: 138
Fragments arranged by size:
3642 1712 1443 274 200 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2470 2502 4825 4857 7533 7565 2470
Size: 32 2323 32 2676 32 2478
Fragments arranged by size:
2676 2478 2323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 4898 1870
Size: 3028 4545
XmnI GAAnn'nnTTC
Cuts at: 2969 3554 3626 5702 2969
Size: 585 72 2076 4840
Fragments arranged by size:
4840 2076 585 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 5381 276
Size: 53 83 186 936 3322 525 2468
Fragments arranged by size:
3322 2468 936 525 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI