Restriction map for
pFN21AA0391
(Circular) (Six-base) MAPSORT of: pFN21AA0391.seq Check: 1192 from: 1 to: 6406
pFN21AA0391 6406 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2325 2601 4469 5853 6121 1632
Size: 693 276 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 693 276 268
AflII C'TTAA_G
Cuts at: 820 1017 2384 3059 3128 820
Size: 197 1367 675 69 4098
Fragments arranged by size:
4098 1367 675 197 69
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 3278 5135 3278
Size: 1857 4549
AleI CACnn'nnGTG
Cuts at: 1166 3599 1166
Size: 2433 3973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 4149 5721 4149
Size: 1572 4834
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2293 2362 2797 3715 3941 4093 6202 1493
Size: 800 69 435 918 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 800 435 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3731 1870
Size: 73 1788 4545
Fragments arranged by size:
4545 1788 73
AvrII C'CTAG_G
Cuts at: 3283 3283
Size: 6406
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 3727 5182 618
Size: 325 217 627 533 1407 1455 1842
Fragments arranged by size:
1842 1455 1407 627 533 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2767 3725 1391
Size: 666 710 958 4072
Fragments arranged by size:
4072 958 710 666
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2639 3648 928
Size: 971 740 1009 3686
Fragments arranged by size:
3686 1009 971 740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2765 2955 4298 5508 2765
Size: 190 1343 1210 3663
Fragments arranged by size:
3663 1343 1210 190
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2082 4467 5623 1790
Size: 292 2385 1156 2573
Fragments arranged by size:
2573 2385 1156 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3310 4137 5095
Size: 416 53 83 399 24 1893 827 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1893 1753 958 827 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 3633 5066 1239
Size: 1047 1347 1433 2579
Fragments arranged by size:
2579 1433 1347 1047
Bpu10I CC'TnA_GC
Cuts at: 2274 2274
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3191 3444 5069 882
Size: 617 1692 253 1625 2219
Fragments arranged by size:
2219 1692 1625 617 253
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2408 4839 5237 5511 5658 6069 2408
Size: 2431 398 274 147 411 2745
Fragments arranged by size:
2745 2431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2143 2173 1178
Size: 30 165 30 740 30 5411
Fragments arranged by size:
5411 740 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 1186
Size: 1198 5208
BseYI C'CCAG_C
Cuts at: 5609 6105 5609
Size: 496 5910
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3401 1163
Size: 573 1665 4168
Fragments arranged by size:
4168 1665 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2767 4467 4552 5623 2767
Size: 1700 85 1071 3550
Fragments arranged by size:
3550 1700 1071 85
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 3250 3250
Size: 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2082 2767 3725 4467 4552 5623
Size: 399 267 25 685 958 742 85 1071
Cuts at: 5623 1391
Size: 2174
Fragments arranged by size:
2174 1071 958 742 685 399 267 85
25
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 2821 3072 4295 6025 2821
Size: 251 1223 1730 3202
Fragments arranged by size:
3202 1730 1223 251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3426 3761 844
Size: 2582 335 3489
Fragments arranged by size:
3489 2582 335
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5050 5237 1700
Size: 110 3240 187 2869
Fragments arranged by size:
3240 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 3619 4466 5478 3619
Size: 847 1012 4547
Fragments arranged by size:
4547 1012 847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 2029 2424 5210 2029
Size: 395 2786 3225
Fragments arranged by size:
3225 2786 395
BstEII G'GTnAC_C
Cuts at: 1184 3095 1184
Size: 1911 4495
BstXI CCAn_nnnn'nTGG
Cuts at: 3681 3681
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401
Size: 1764 367 385 17 1452 86 12 346
Cuts at: 6401 1972
Size: 1977
Fragments arranged by size:
1977 1764 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3120 3120
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 3413 3430 3922 4716 4736 3413
Size: 17 492 794 20 5083
Fragments arranged by size:
5083 794 492 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 2397 2850 3710 2397
Size: 453 860 5093
Fragments arranged by size:
5093 860 453
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3853 4742
Size: 54 1425 50 275 18 1162 861 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1162 889 861 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3333 4336 1353
Size: 692 1288 1003 3423
Fragments arranged by size:
3423 1288 1003 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2765 2765
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2325 2601 3633 4469 5066 5853
Size: 393 654 39 276 1032 836 597 787
Cuts at: 5853 6121 1239
Size: 268 1524
Fragments arranged by size:
1524 1032 836 787 654 597 393 276
268 39
EcoRI G'AATT_C
Cuts at: 2293 2362 3715 4093 2293
Size: 69 1353 378 4606
Fragments arranged by size:
4606 1353 378 69
EcoRV GAT'ATC
Cuts at: 2204 2204
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 3103 3135 3264
Size: 32 135 32 709 32 790 32 129
Cuts at: 3264 3296 3365 3397 5047 5079 5121 5153 1373
Size: 32 69 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 790 709 135 129 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2032 2042 2853 748
Size: 1284 10 811 4301
Fragments arranged by size:
4301 1284 811 10
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 3537 4005 3537
Size: 468 5938
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2411 2820 5520 5704 1305
Size: 1106 409 2700 184 2007
Fragments arranged by size:
2700 2007 1106 409 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2994 10
Size: 54 1425 343 1162 3422
Fragments arranged by size:
3422 1425 1162 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2778 3599 4364 4723 4882 518
Size: 648 919 693 821 765 359 159 2042
Fragments arranged by size:
2042 919 821 765 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2941 3034 3360 3532 3822 4499 5647
Size: 506 829 93 326 172 290 677 1148
Cuts at: 5647 5892 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 829 677 506 326 290 245
172 93
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 2568 3634 387
Size: 2181 1066 3159
Fragments arranged by size:
3159 2181 1066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3301 3764 5309 1132
Size: 97 2072 463 1545 2229
Fragments arranged by size:
2229 2072 1545 463 97
PasI CC'CwG_GG
Cuts at: 1348 2075 1348
Size: 727 5679
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2063 1108
Size: 955 5451
PfoI T'CCnGG_A
Cuts at: 3422 3553 3422
Size: 131 6275
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 2723 3976 2723
Size: 1253 5153
PstI C_TGCA'G
Cuts at: 830 3437 3758 830
Size: 2607 321 3478
Fragments arranged by size:
3478 2607 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2941 3034 3360 3532 2941
Size: 93 326 172 5815
Fragments arranged by size:
5815 326 172 93
SacI G_AGCT'C
Cuts at: 2767 2767
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2466 3433 3754 4889 5570 5761 826
Size: 219 1421 967 321 1135 681 191 1471
Fragments arranged by size:
1471 1421 1135 967 681 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3059 3128 4510 5411 5673
Size: 197 926 441 675 69 1382 901 262
Cuts at: 5673 820
Size: 1553
Fragments arranged by size:
1553 1382 926 901 675 441 262 197
69
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 3473 1517
Size: 1956 4450
StyI C'CwwG_G
Cuts at: 513 1065 2062 2193 2316 3283 3845 513
Size: 552 997 131 123 967 562 3074
Fragments arranged by size:
3074 997 967 562 552 131 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4433 4450 4603 4788 5233 325
Size: 1255 2853 17 153 185 445 1498
Fragments arranged by size:
2853 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3102 4652 6364
Size: 274 80 33 51 1443 1125 1550 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
1712 1550 1443 1125 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI