Restriction map for
pFN21AA0386
(Circular) (Six-base) MAPSORT of: pFN21AA0386.seq Check: 5282 from: 1 to: 7909
pFN21AA0386 7909 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5719 278
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2235 2901 4482 5252 1937
Size: 298 666 1581 770 4594
Fragments arranged by size:
4594 1581 770 666 298
Acc65I G'GTAC_C
Cuts at: 2918 3041 5230 2918
Size: 123 2189 5597
Fragments arranged by size:
5597 2189 123
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2996 3163 3286 3524 3804 4179 5088
Size: 548 816 167 123 238 280 375 909
Cuts at: 5088 5211 5972 7356 7624 1632
Size: 123 761 1384 268 1917
Fragments arranged by size:
1917 1384 909 816 761 548 375 280
268 238 167 123 123
AfeI AGC'GCT
Cuts at: 4152 4152
Size: 7909
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7712
AflIII A'CryG_T
Cuts at: 1203 6808 7785 1203
Size: 5605 977 1327
Fragments arranged by size:
5605 1327 977
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 5052 6638 5052
Size: 1586 6323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5054 1184
Size: 3870 4039
AlwNI CAG_nnn'CTG
Cuts at: 2990 3581 3886 4088 4750 5652 7224 2990
Size: 591 305 202 662 902 1572 3675
Fragments arranged by size:
3675 1572 902 662 591 305 202
ApaI G_GGCC'C
Cuts at: 2043 2043
Size: 7909
ApaLI G'TGCA_C
Cuts at: 2575 5966 7122 2575
Size: 3391 1156 3362
Fragments arranged by size:
3391 3362 1156
ApoI r'AATT_y
Cuts at: 1493 2125 2612 2676 3904 4421 4596 5147 5218
Size: 632 487 64 1228 517 175 551 71
Cuts at: 5218 5444 5596 7705 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1228 632 551 517 487 226
175 152 71 64
AseI AT'TA_AT
Cuts at: 160 1032 6463 160
Size: 872 5431 1606
Fragments arranged by size:
5431 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 2520 5154 5234 1870
Size: 73 577 2634 80 4545
Fragments arranged by size:
4545 2634 577 80 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2902 2935 2902
Size: 33 7876
BamHI G'GATC_C
Cuts at: 2015 5239 5606 2015
Size: 3224 367 4318
Fragments arranged by size:
4318 3224 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 2918 3041 3730 4245
Size: 325 217 627 681 450 123 689 515
Cuts at: 4245 5230 6685 618
Size: 985 1455 1842
Fragments arranged by size:
1842 1455 985 689 681 627 515 450
325 217 123
BanII G_rGCy'C
Cuts at: 1391 2043 2814 3228 3528 3657 5228 1391
Size: 652 771 414 300 129 1571 4072
Fragments arranged by size:
4072 1571 771 652 414 300 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3519 3945 4167 928
Size: 971 1620 426 222 4670
Fragments arranged by size:
4670 1620 971 426 222
BbvCI CC'TCA_GC
Cuts at: 2211 3407 2211
Size: 1196 6713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 6100 6134 1903
Size: 34 2312 34 1817 34 3678
Fragments arranged by size:
3678 2312 1817 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2456 2961 5801 7011 2456
Size: 505 2840 1210 3354
Fragments arranged by size:
3354 2840 1210 505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 3115 6520
Size: 107 122 71 631 605 366 1077 3405
Cuts at: 6520 136
Size: 1525
Fragments arranged by size:
3405 1525 1077 631 605 366 122 107
71
BglII A'GATC_T
Cuts at: 7904 7904
Size: 7909
BlpI GC'TnA_GC
Cuts at: 3296 3451 3588 4611 5326 3296
Size: 155 137 1023 715 5879
Fragments arranged by size:
5879 1023 715 155 137
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2579 5970 7126 1790
Size: 253 536 3391 1156 2573
Fragments arranged by size:
3391 2573 1156 536 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2014 4565 5640
Size: 416 53 83 399 24 597 2551 1075
Cuts at: 5640 6598 442
Size: 958 1753
Fragments arranged by size:
2551 1753 1075 958 597 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2825 1056
Size: 1769 6140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2072 2291 2420 2444 2682 4235 4538
Size: 816 17 219 129 24 238 1553 303
Cuts at: 4538 4612 4721 4961 4967 6569 1239
Size: 74 109 240 6 1602 2579
Fragments arranged by size:
2579 1602 1553 816 303 240 238 219
129 109 74 24 17 6
Bpu10I CC'TnA_GC
Cuts at: 2211 3407 4712 2211
Size: 1196 1305 5408
Fragments arranged by size:
5408 1305 1196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2123 3148 3623 3641 4273 5036 6034 6899 7197
Size: 1025 475 18 632 763 998 865 298
Cuts at: 7197 2123
Size: 2835
Fragments arranged by size:
2835 1025 998 865 763 632 475 298
18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2852 6572 882
Size: 617 1353 3720 2219
Fragments arranged by size:
3720 2219 1353 617
BsaAI yAC'GTr
Cuts at: 493 1204 5700 493
Size: 711 4496 2702
Fragments arranged by size:
4496 2702 711
BsaBI GATnn'nnATC
Cuts at: 2265 7903 2265
Size: 5638 2271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2395 2455 4689 5716
Size: 53 83 186 936 862 60 2234 1027
Cuts at: 5716 6098 6733 275
Size: 382 635 1451
Fragments arranged by size:
2234 1451 1027 936 862 635 382 186
83 60 53
BsaWI w'CCGG_w
Cuts at: 6342 6740 7014 7161 7572 6342
Size: 398 274 147 411 6679
Fragments arranged by size:
6679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3176 3206 1178
Size: 30 165 30 1773 30 5881
Fragments arranged by size:
5881 1773 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3031 3290 3536 3656 4040 4043 1186
Size: 1845 259 246 120 384 3 5052
Fragments arranged by size:
5052 1845 384 259 246 120 3
BseYI C'CCAG_C
Cuts at: 2605 2745 2990 3581 4492 4802 4818 7112 7608
Size: 140 245 591 911 310 16 2294 496
Cuts at: 7608 2605
Size: 2906
Fragments arranged by size:
2906 2294 911 591 496 310 245 140
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3146 4243 4292 1163
Size: 573 1410 1097 49 4780
Fragments arranged by size:
4780 1410 1097 573 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5359 6120 7148 1540
Size: 275 186 3358 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2579 2814 3228 3594 3657 5970 6055 7126 2579
Size: 235 414 366 63 2313 85 1071 3362
Fragments arranged by size:
3362 2313 1071 414 366 235 85 63
BsmI GAATG_Cn'
Cuts at: 5420 5513 5420
Size: 93 7816
BsmBI CGTCTCn'nnnn_
Cuts at: 2386 2386
Size: 7909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2579 2814 3228 3528 3594 3657
Size: 399 253 536 235 414 300 66 63
Cuts at: 3657 5228 5970 6055 7126 1391
Size: 1571 742 85 1071 2174
Fragments arranged by size:
2174 1571 1071 742 536 414 399 300
253 235 85 66 63
BspEI T'CCGG_A
Cuts at: 7572 7572
Size: 7909
BspHI T'CATG_A
Cuts at: 2682 3900 5798 7528 2682
Size: 1218 1898 1730 3063
Fragments arranged by size:
3063 1898 1730 1218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3195 3318 3544 3939 5264 844
Size: 2351 123 226 395 1325 3489
Fragments arranged by size:
3489 2351 1325 395 226 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2135 3174 5796 1394
Size: 429 312 1039 2622 3507
Fragments arranged by size:
3507 2622 1039 429 312
BsrDI GCAATG_nn'
Cuts at: 65 1248 6404 6578 65
Size: 1183 5156 174 1396
Fragments arranged by size:
5156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2694 6553 6740 1700
Size: 110 884 3859 187 2869
Fragments arranged by size:
3859 2869 884 187 110
BsrGI T'GTAC_A
Cuts at: 96 3030 96
Size: 2934 4975
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7909
BssSI C'ACGA_G
Cuts at: 4268 5969 6981 4268
Size: 1701 1012 5196
Fragments arranged by size:
5196 1701 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7909
BstBI TT'CG_AA
Cuts at: 4579 6713 4579
Size: 2134 5775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 7909
BstYI r'GATC_y
Cuts at: 1972 2015 3794 3883 4341 5239 5606 5991 6008
Size: 43 1779 89 458 898 367 385 17
Cuts at: 6008 7460 7546 7558 7904 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1779 1452 898 458 385 367 346
89 86 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 2148 2886 2983 3197 3574 2148
Size: 738 97 214 377 6483
Fragments arranged by size:
6483 738 377 214 97
BtgI C'CryG_G
Cuts at: 513 1065 4684 4737 513
Size: 552 3619 53 3685
Fragments arranged by size:
3685 3619 552 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7170
Fragments arranged by size:
7170 683 56
BtsI GCAGTG_nn'
Cuts at: 5425 6219 6239 5425
Size: 794 20 7095
Fragments arranged by size:
7095 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5188 5223 529
Size: 35 4624 35 3215
Fragments arranged by size:
4624 3215 35 35
DraI TTT'AAA
Cuts at: 5213 5213
Size: 7909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6916 809
Size: 507 5600 1802
Fragments arranged by size:
5600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2692 3738 4722
Size: 54 1425 50 275 18 862 1046 984
Cuts at: 4722 5356 6245 7874 8
Size: 634 889 1629 43
Fragments arranged by size:
1629 1425 1046 984 889 862 634 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5356 1537
Size: 275 3544 4090
Fragments arranged by size:
4090 3544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5839 1353
Size: 4486 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4074 6496 6870 7016 387
Size: 1064 138 2485 2422 374 146 1280
Fragments arranged by size:
2485 2422 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 3226 3655 2812
Size: 414 429 7066
Fragments arranged by size:
7066 429 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2072 2180 2291 2420 2444 2682
Size: 393 423 17 108 111 129 24 238
Cuts at: 2682 2996 3163 3286 3524 3804 4179 4235 4538
Size: 314 167 123 238 280 375 56 303
Cuts at: 4538 4612 4721 4961 4967 5088 5211 5972 6569
Size: 74 109 240 6 121 123 761 597
Cuts at: 6569 7356 7624 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 761 597 423 393 375 314
303 280 268 240 238 238 167 129
123 123 121 111 109 108 74 56
24 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3079 3196 3506 3079
Size: 117 310 7482
Fragments arranged by size:
7482 310 117
EcoO109I rG'GnC_Cy
Cuts at: 2039 2241 2039
Size: 202 7707
EcoRI G'AATT_C
Cuts at: 2612 5218 5596 2612
Size: 2606 378 4925
Fragments arranged by size:
4925 2606 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 7909
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 2991 4154 7056 1678
Size: 1313 1163 2902 2531
Fragments arranged by size:
2902 2531 1313 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 3176 3208 3434
Size: 32 135 32 1045 32 527 32 226
Cuts at: 3434 3466 3479 3511 4828 4860 5038 5070 6550
Size: 32 13 32 1317 32 178 32 1480
Cuts at: 6550 6582 6624 6656 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1480 1317 1045 527 226 178 135
42 32 32 32 32 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2301 2902 4543 5253 5499 669
Size: 988 281 363 601 1641 710 246 3079
Fragments arranged by size:
3079 1641 988 710 601 363 281 246
HindIII A'AGCT_T
Cuts at: 748 2436 3811 3991 4328 748
Size: 1688 1375 180 337 4329
Fragments arranged by size:
4329 1688 1375 337 180
HpaI GTT'AAC
Cuts at: 5499 5499
Size: 7909
KpnI G_GTAC'C
Cuts at: 2922 3045 5234 2922
Size: 123 2189 5597
Fragments arranged by size:
5597 2189 123
MfeI C'AATT_G
Cuts at: 2376 5138 5508 2376
Size: 2762 370 4777
Fragments arranged by size:
4777 2762 370
MluI A'CGCG_T
Cuts at: 7785 7785
Size: 7909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3384 5152 7023 7207 1305
Size: 2079 1768 1871 184 2007
Fragments arranged by size:
2079 2007 1871 1768 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3740 4724 10
Size: 54 1425 343 1908 984 3195
Fragments arranged by size:
3195 1908 1425 984 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2624 4143 5036 5867 6226 6385
Size: 648 1419 39 1519 893 831 359 159
Cuts at: 6385 518
Size: 2042
Fragments arranged by size:
2042 1519 1419 893 831 648 359 159
39
MspA1I CmG'CkG
Cuts at: 1606 2082 2701 3703 4367 4496 4516 4720 5325
Size: 476 619 1002 664 129 20 204 605
Cuts at: 5325 6002 7150 7395 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1002 677 664 619 605 476
245 204 129 20
NaeI GCC'GGC
Cuts at: 2696 2696
Size: 7909
NcoI C'CATG_G
Cuts at: 513 1065 4737 513
Size: 552 3672 3685
Fragments arranged by size:
3685 3672 552
NdeI CA'TA_TG
Cuts at: 387 5131 387
Size: 4744 3165
NgoMIV G'CCGG_C
Cuts at: 2694 2694
Size: 7909
NheI G'CTAG_C
Cuts at: 1052 2821 1052
Size: 1769 6140
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NspI r_CATG'y
Cuts at: 1132 1229 5267 6812 1132
Size: 97 4038 1545 2229
Fragments arranged by size:
4038 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3472 1348
Size: 2124 5785
PciI A'CATG_T
Cuts at: 6808 6808
Size: 7909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4351 4600 1108
Size: 3243 249 4417
Fragments arranged by size:
4417 3243 249
PfoI T'CCnGG_A
Cuts at: 2088 2460 2659 3461 4555 2088
Size: 372 199 802 1094 5442
Fragments arranged by size:
5442 1094 802 372 199
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5978 6010 7491 7523 3581
Size: 32 2365 32 1481 32 3967
Fragments arranged by size:
3967 2365 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 5479 5479
Size: 7909
PspOMI G'GGCC_C
Cuts at: 2039 2039
Size: 7909
PstI C_TGCA'G
Cuts at: 830 3727 3794 4469 5261 830
Size: 2897 67 675 792 3478
Fragments arranged by size:
3478 2897 792 675 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
PvuII CAG'CTG
Cuts at: 3703 4367 4516 4720 3703
Size: 664 149 204 6892
Fragments arranged by size:
6892 664 204 149
SacI G_AGCT'C
Cuts at: 2814 3228 3657 2814
Size: 414 429 7066
Fragments arranged by size:
7066 429 414
SalI G'TCGA_C
Cuts at: 1936 2900 5251 1936
Size: 964 2351 4594
Fragments arranged by size:
4594 2351 964
SbfI CC_TGCA'GG
Cuts at: 5261 5261
Size: 7909
ScaI AGT'ACT
Cuts at: 4256 4830 6157 7869 4256
Size: 574 1327 1712 4296
Fragments arranged by size:
4296 1712 1327 574
SexAI A'CCwGG_T
Cuts at: 2945 2945
Size: 7909
SfcI C'TryA_G
Cuts at: 826 1045 2236 3723 3790 4465 5257 6392 7073
Size: 219 1191 1487 67 675 792 1135 681
Cuts at: 7073 7264 826
Size: 191 1471
Fragments arranged by size:
1487 1471 1191 1135 792 681 675 219
191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 2522 5236 1872
Size: 650 2714 4545
Fragments arranged by size:
4545 2714 650
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 3127 3602 3656 4288 5015
Size: 197 926 195 989 475 54 632 727
Cuts at: 5015 5154 6013 6914 7176 820
Size: 139 859 901 262 1553
Fragments arranged by size:
1553 989 926 901 859 727 632 475
262 197 195 139 54
SnaBI TAC'GTA
Cuts at: 493 5700 493
Size: 5207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 5267 1132
Size: 4135 3774
SspI AAT'ATT
Cuts at: 5 52 5833 5
Size: 47 5781 2081
Fragments arranged by size:
5781 2081 47
StuI AGG'CCT
Cuts at: 1517 3647 4937 1517
Size: 2130 1290 4489
Fragments arranged by size:
4489 2130 1290
StyI C'CwwG_G
Cuts at: 513 1065 2914 3210 4737 5348 513
Size: 552 1849 296 1527 611 3074
Fragments arranged by size:
3074 1849 1527 611 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 4137 4277 5936 5953 6106 6291
Size: 1255 750 1807 140 1659 17 153 185
Cuts at: 6291 6736 325
Size: 445 1498
Fragments arranged by size:
1807 1659 1498 1255 750 445 185 153
140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 2559 3030
Size: 274 80 33 51 1443 367 215 471
Cuts at: 3030 4254 4828 6155 7867 96
Size: 1224 574 1327 1712 138
Fragments arranged by size:
1712 1443 1327 1224 574 471 367 274
215 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2935 2967 7869 7901 2935
Size: 32 4902 32 2943
Fragments arranged by size:
4902 2943 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7909
XbaI T'CTAG_A
Cuts at: 3802 5245 3802
Size: 1443 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3469 4810 3469
Size: 1341 6568
XhoI C'TCGA_G
Cuts at: 1943 5154 1943
Size: 3211 4698
XmaI C'CCGG_G
Cuts at: 1870 2520 5234 1870
Size: 650 2714 4545
Fragments arranged by size:
4545 2714 650
XmnI GAAnn'nnTTC
Cuts at: 3624 6038 3624
Size: 2414 5495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5717 276
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI