Restriction map for
pFN21AA0381
(Circular) (Six-base) MAPSORT of: pFN21AA0381.seq Check: 8121 from: 1 to: 7909
pFN21AA0381 7909 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3777 5719 278
Size: 53 83 186 936 2241 1942 2468
Fragments arranged by size:
2468 2241 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2929 5252 1937
Size: 992 2323 4594
Fragments arranged by size:
4594 2323 992
Acc65I G'GTAC_C
Cuts at: 2802 5230 2802
Size: 2428 5481
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 3125 3839 3996 4569 4753 5009 5972
Size: 791 702 714 157 573 184 256 963
Cuts at: 5972 7356 7624 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 963 791 714 702 573 268
256 184 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7712
AflIII A'CryG_T
Cuts at: 1203 2352 2581 6808 7785 1203
Size: 1149 229 4227 977 1327
Fragments arranged by size:
4227 1327 1149 977 229
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 4498 5083 6638 4498
Size: 585 1555 5769
Fragments arranged by size:
5769 1555 585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3755 1184
Size: 2571 5338
AlwNI CAG_nnn'CTG
Cuts at: 2347 2770 3205 3493 3557 4279 4610 5167 5652
Size: 423 435 288 64 722 331 557 485
Cuts at: 5652 7224 2347
Size: 1572 3032
Fragments arranged by size:
3032 1572 722 557 485 435 423 331
288 64
ApaI G_GGCC'C
Cuts at: 3587 4039 3587
Size: 452 7457
ApaLI G'TGCA_C
Cuts at: 5966 7122 5966
Size: 1156 6753
ApoI r'AATT_y
Cuts at: 1493 2479 2912 4304 4871 5218 5444 5596 7705
Size: 986 433 1392 567 347 226 152 2109
Cuts at: 7705 1493
Size: 1697
Fragments arranged by size:
2109 1697 1392 986 567 433 347 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6463 160
Size: 872 5431 1606
Fragments arranged by size:
5431 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 3477 3504 3689 4362 5178 5234 1870
Size: 73 1534 27 185 673 816 56 4545
Fragments arranged by size:
4545 1534 816 673 185 73 56 27
BamHI G'GATC_C
Cuts at: 3903 5239 5606 3903
Size: 1336 367 6206
Fragments arranged by size:
6206 1336 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 3713 3863 3951 4451
Size: 325 217 627 1015 911 150 88 500
Cuts at: 4451 4701 5230 6685 618
Size: 250 529 1455 1842
Fragments arranged by size:
1842 1455 1015 911 627 529 500 325
250 217 150 88
BanII G_rGCy'C
Cuts at: 1391 2148 3587 3971 4039 4719 5228 1391
Size: 757 1439 384 68 680 509 4072
Fragments arranged by size:
4072 1439 757 680 509 384 68
BbeI G_GCGC'C
Cuts at: 4455 4705 4455
Size: 250 7659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 2411 3591 3984 5101 928
Size: 971 504 8 1180 393 1117 3736
Fragments arranged by size:
3736 1180 1117 971 504 393 8
BbvCI CC'TCA_GC
Cuts at: 4323 4323
Size: 7909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3881 3915 5031 5065 6100 6134 1903
Size: 34 1944 34 1116 34 1035 34 3678
Fragments arranged by size:
3678 1944 1116 1035 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5801 7011 5801
Size: 1210 6699
BclI T'GATC_A
Cuts at: 1548 4539 1548
Size: 2991 4918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2031 6520 136
Size: 107 122 71 631 605 359 4489 1525
Fragments arranged by size:
4489 1525 631 605 359 122 107 71
BglII A'GATC_T
Cuts at: 2241 7904 2241
Size: 5663 2246
BlpI GC'TnA_GC
Cuts at: 5326 5326
Size: 7909
Bme1580I G_kGCm'C
Cuts at: 1790 2581 3587 3718 4039 5970 7126 1790
Size: 791 1006 131 321 1931 1156 2573
Fragments arranged by size:
2573 1931 1156 1006 791 321 131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3841 4713 5640
Size: 416 53 83 399 24 2424 872 927
Cuts at: 5640 6598 442
Size: 958 1753
Fragments arranged by size:
2424 1753 958 927 872 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3032 1056
Size: 1976 5933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3985 3985
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2104 2477 2684 2886 2892 2906 3044 3203
Size: 865 373 207 202 6 14 138 159
Cuts at: 3203 3218 3410 4163 4211 4571 4655 4709 4805
Size: 15 192 753 48 360 84 54 96
Cuts at: 4805 5075 6569 1239
Size: 270 1494 2579
Fragments arranged by size:
2579 1494 865 753 373 360 270 207
202 192 159 138 96 84 54 48
15 14 6
Bpu10I CC'TnA_GC
Cuts at: 4131 4323 4131
Size: 192 7717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4139 5117 6034 6899 7197 4139
Size: 978 917 865 298 4851
Fragments arranged by size:
4851 978 917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3333 6572 882
Size: 617 1834 3239 2219
Fragments arranged by size:
3239 2219 1834 617
BsaAI yAC'GTr
Cuts at: 493 1204 5700 493
Size: 711 4496 2702
Fragments arranged by size:
4496 2702 711
BsaBI GATnn'nnATC
Cuts at: 4544 7903 4544
Size: 3359 4550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3774 4452 4702 5716
Size: 53 83 186 936 2241 678 250 1014
Cuts at: 5716 6098 6733 275
Size: 382 635 1451
Fragments arranged by size:
2241 1451 1014 936 678 635 382 250
186 83 53
BsaWI w'CCGG_w
Cuts at: 2415 2487 3357 6342 6740 7014 7161 7572 2415
Size: 72 870 2985 398 274 147 411 2752
Fragments arranged by size:
2985 2752 870 411 398 274 147 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4676 4706 1178
Size: 30 165 30 3273 30 4381
Fragments arranged by size:
4381 3273 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2156 2378 2381 3627 3630 3863 4097
Size: 849 121 222 3 1246 3 233 234
Cuts at: 4097 4142 4208 4954 4957 4967 4970 4973 5072
Size: 45 66 746 3 10 3 3 99
Cuts at: 5072 5079 1186
Size: 7 4016
Fragments arranged by size:
4016 1246 849 746 234 233 222 121
99 66 45 10 7 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2923 3531 3764 3801 3964 7112 7608 2923
Size: 608 233 37 163 3148 496 3224
Fragments arranged by size:
3224 3148 608 496 233 163 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2711 3128 1163
Size: 573 975 417 5944
Fragments arranged by size:
5944 975 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 5359 6120 7148 1540
Size: 275 186 316 3042 761 1028 2301
Fragments arranged by size:
3042 2301 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 4200 5970 6055 7126 2148
Size: 2052 1770 85 1071 2931
Fragments arranged by size:
2931 2052 1770 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 5420 5513 3413
Size: 2007 93 5809
Fragments arranged by size:
5809 2007 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2363 2363
Size: 7909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2581 3587 3718 3971 4039 4200
Size: 399 358 433 1006 131 253 68 161
Cuts at: 4200 4719 5228 5970 6055 7126 1391
Size: 519 509 742 85 1071 2174
Fragments arranged by size:
2174 1071 1006 742 519 509 433 399
358 253 161 131 85 68
BspEI T'CCGG_A
Cuts at: 2415 2487 3357 7572 2415
Size: 72 870 4215 2752
Fragments arranged by size:
4215 2752 870 72
BspHI T'CATG_A
Cuts at: 4428 4542 4740 5798 7528 4428
Size: 114 198 1058 1730 4809
Fragments arranged by size:
4809 1730 1058 198 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3630 3883 5264 844
Size: 2786 253 1381 3489
Fragments arranged by size:
3489 2786 1381 253
BsrBI CCG'CTC
Cuts at: 1394 1823 3244 3403 4216 4315 4673 4963 5011
Size: 429 1421 159 813 99 358 290 48
Cuts at: 5011 5185 5796 1394
Size: 174 611 3507
Fragments arranged by size:
3507 1421 813 611 429 358 290 174
159 99 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 6404 6578 65
Size: 1183 5156 174 1396
Fragments arranged by size:
5156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2799 5196 6553 6740 1700
Size: 110 989 2397 1357 187 2869
Fragments arranged by size:
2869 2397 1357 989 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7909
BssHII G'CGCG_C
Cuts at: 1925 4970 1925
Size: 3045 4864
BssSI C'ACGA_G
Cuts at: 2687 5969 6981 2687
Size: 3282 1012 3615
Fragments arranged by size:
3615 3282 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2722 4279 794
Size: 1928 1557 4424
Fragments arranged by size:
4424 1928 1557
BstBI TT'CG_AA
Cuts at: 6713 6713
Size: 7909
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 4354 2284
Size: 2070 5839
BstYI r'GATC_y
Cuts at: 1972 2182 2241 2667 3903 4086 4924 5239 5606
Size: 210 59 426 1236 183 838 315 367
Cuts at: 5606 5991 6008 7460 7546 7558 7904 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1236 838 426 385 367 346
315 210 183 86 59 17 12
BstZ17I GTA'TAC
Cuts at: 2930 2930
Size: 7909
Bsu36I CC'TnA_GG
Cuts at: 2598 3750 4655 2598
Size: 1152 905 5852
Fragments arranged by size:
5852 1152 905
BtgI C'CryG_G
Cuts at: 513 1065 2004 2029 2328 513
Size: 552 939 25 299 6094
Fragments arranged by size:
6094 939 552 299 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2351 487
Size: 683 56 1125 6045
Fragments arranged by size:
6045 1125 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 2216 3148 3200 3775 5425 6219 6239 2068
Size: 148 932 52 575 1650 794 20 3738
Fragments arranged by size:
3738 1650 932 794 575 148 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3576 3611 529
Size: 35 3012 35 4827
Fragments arranged by size:
4827 3012 35 35
DraI TTT'AAA
Cuts at: 5213 5213
Size: 7909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4730 6916 809
Size: 507 3414 2186 1802
Fragments arranged by size:
3414 2186 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2698 4027 4470
Size: 54 1425 50 275 18 868 1329 443
Cuts at: 4470 5356 6245 7874 8
Size: 886 889 1629 43
Fragments arranged by size:
1629 1425 1329 889 886 868 443 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5356 1537
Size: 275 3544 4090
Fragments arranged by size:
4090 3544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3429 4166 4295 5839 1353
Size: 2076 737 129 1544 3423
Fragments arranged by size:
3423 2076 1544 737 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2882 6496 6870 7016 387
Size: 1064 138 1293 3614 374 146 1280
Fragments arranged by size:
3614 1293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 7909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2423 2477 2684 2886 2892 2906
Size: 393 472 319 54 207 202 6 14
Cuts at: 2906 3044 3125 3203 3218 3410 3839 3996 4163
Size: 138 81 78 15 192 429 157 167
Cuts at: 4163 4211 4569 4571 4655 4709 4753 4805 5009
Size: 48 358 2 84 54 44 52 204
Cuts at: 5009 5075 5972 6569 7356 7624 1239
Size: 66 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 472 429 393 358
319 268 207 204 202 192 167 157
138 84 81 78 66 54 54 52
48 44 15 14 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3749 3946 4130 4190 3749
Size: 197 184 60 7468
Fragments arranged by size:
7468 197 184 60
EcoO109I rG'GnC_Cy
Cuts at: 2272 3554 3583 3695 3754 4035 4123 4663 5032
Size: 1282 29 112 59 281 88 540 369
Cuts at: 5032 5164 2272
Size: 132 5017
Fragments arranged by size:
5017 1282 540 369 281 132 112 88
59 29
EcoRI G'AATT_C
Cuts at: 2912 4871 5218 5596 2912
Size: 1959 347 378 5225
Fragments arranged by size:
5225 1959 378 347
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 4455 4705 7056 1678
Size: 2777 250 2351 2531
Fragments arranged by size:
2777 2531 2351 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2329 2361 4484 4516 5069
Size: 32 135 32 757 32 2123 32 553
Cuts at: 5069 5101 6550 6582 6624 6656 1373
Size: 32 1449 32 42 32 2626
Fragments arranged by size:
2626 2123 1449 757 553 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3301 4621 5253 5499 669
Size: 988 281 1363 1320 632 246 3079
Fragments arranged by size:
3079 1363 1320 988 632 281 246
HindIII A'AGCT_T
Cuts at: 748 4070 748
Size: 3322 4587
HpaI GTT'AAC
Cuts at: 5499 5499
Size: 7909
KasI G'GCGC_C
Cuts at: 4451 4701 4451
Size: 250 7659
KpnI G_GTAC'C
Cuts at: 2806 5234 2806
Size: 2428 5481
MfeI C'AATT_G
Cuts at: 5508 5508
Size: 7909
MluI A'CGCG_T
Cuts at: 2352 7785 2352
Size: 5433 2476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7023 7207 1305
Size: 5718 184 2007
Fragments arranged by size:
5718 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2700 4472 10
Size: 54 1425 343 868 1772 3447
Fragments arranged by size:
3447 1772 1425 868 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 4203 4757 5867 6226 6385 518
Size: 648 1798 1239 554 1110 359 159 2042
Fragments arranged by size:
2042 1798 1239 1110 648 554 359 159
MspA1I CmG'CkG
Cuts at: 1606 2295 5020 5026 5325 6002 7150 7395 1606
Size: 689 2725 6 299 677 1148 245 2120
Fragments arranged by size:
2725 2120 1148 689 677 299 245 6
NarI GG'CG_CC
Cuts at: 4452 4702 4452
Size: 250 7659
NcoI C'CATG_G
Cuts at: 513 1065 2004 2029 2328 513
Size: 552 939 25 299 6094
Fragments arranged by size:
6094 939 552 299 25
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7909
NheI G'CTAG_C
Cuts at: 1052 3028 1052
Size: 1976 5933
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NspI r_CATG'y
Cuts at: 1132 1229 2585 3307 3892 4141 4582 5267 6812
Size: 97 1356 722 585 249 441 685 1545
Cuts at: 6812 1132
Size: 2229
Fragments arranged by size:
2229 1545 1356 722 685 585 441 249
97
PasI CC'CwG_GG
Cuts at: 1348 2569 3578 1348
Size: 1221 1009 5679
Fragments arranged by size:
5679 1221 1009
PciI A'CATG_T
Cuts at: 2581 6808 2581
Size: 4227 3682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2035 2392 3578 5167 1108
Size: 927 357 1186 1589 3850
Fragments arranged by size:
3850 1589 1186 927 357
PfoI T'CCnGG_A
Cuts at: 2085 2973 3204 3234 3906 4188 4308 4548 2085
Size: 888 231 30 672 282 120 240 5446
Fragments arranged by size:
5446 888 672 282 240 231 120 30
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5978 6010 7491 7523 5978
Size: 32 1481 32 6364
Fragments arranged by size:
6364 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 3695 3754 4123 5032 2272
Size: 1423 59 369 909 5149
Fragments arranged by size:
5149 1423 909 369 59
PshAI GACnn'nnGTC
Cuts at: 1613 2790 1613
Size: 1177 6732
PsiI TTA'TAA
Cuts at: 5479 5479
Size: 7909
PspOMI G'GGCC_C
Cuts at: 3583 4035 3583
Size: 452 7457
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 7877
PstI C_TGCA'G
Cuts at: 830 2106 2252 2718 3159 5044 5261 830
Size: 1276 146 466 441 1885 217 3478
Fragments arranged by size:
3478 1885 1276 466 441 217 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
PvuII CAG'CTG
Cuts at: 2295 2295
Size: 7909
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 7909
SalI G'TCGA_C
Cuts at: 1936 5251 1936
Size: 3315 4594
SanDI GG'GwC_CC
Cuts at: 3695 3695
Size: 7909
SbfI CC_TGCA'GG
Cuts at: 5261 5261
Size: 7909
ScaI AGT'ACT
Cuts at: 6157 7869 6157
Size: 1712 6197
SexAI A'CCwGG_T
Cuts at: 2733 5079 2733
Size: 2346 5563
SfcI C'TryA_G
Cuts at: 826 1045 2102 2248 2714 3155 5040 5257 6392
Size: 219 1057 146 466 441 1885 217 1135
Cuts at: 6392 7073 7264 826
Size: 681 191 1471
Fragments arranged by size:
1885 1471 1135 1057 681 466 441 219
217 191 146
SfoI GGC'GCC
Cuts at: 4453 4703 4453
Size: 250 7659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 3479 3691 4364 5180 5236 1872
Size: 1607 212 673 816 56 4545
Fragments arranged by size:
4545 1607 816 673 212 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4154 5132 6013 6914 7176 820
Size: 197 926 2211 978 881 901 262 1553
Fragments arranged by size:
2211 1553 978 926 901 881 262 197
SnaBI TAC'GTA
Cuts at: 493 5700 493
Size: 5207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 5267 1132
Size: 4135 3774
SspI AAT'ATT
Cuts at: 5 52 5833 5
Size: 47 5781 2081
Fragments arranged by size:
5781 2081 47
StuI AGG'CCT
Cuts at: 1517 3119 4826 4904 1517
Size: 1602 1707 78 4522
Fragments arranged by size:
4522 1707 1602 78
StyI C'CwwG_G
Cuts at: 513 1065 2004 2029 2328 2649 3270 4821 5348
Size: 552 939 25 299 321 621 1551 527
Cuts at: 5348 513
Size: 3074
Fragments arranged by size:
3074 1551 939 621 552 527 321 299
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 2802 3823 3828 4006 5936 5953
Size: 1255 1083 139 1021 5 178 1930 17
Cuts at: 5953 6106 6291 6736 325
Size: 153 185 445 1498
Fragments arranged by size:
1930 1498 1255 1083 1021 445 185 178
153 139 17 5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3108 6155 7867
Size: 274 80 33 51 1443 1131 3047 1712
Cuts at: 7867 96
Size: 138
Fragments arranged by size:
3047 1712 1443 1131 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3778 3810 4210 4242 7869 7901 3778
Size: 32 400 32 3627 32 3786
Fragments arranged by size:
3786 3627 400 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3047 1316
Size: 1731 6178
XbaI T'CTAG_A
Cuts at: 4927 5245 4927
Size: 318 7591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4105 4105
Size: 7909
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7909
XmaI C'CCGG_G
Cuts at: 1870 3477 3689 4362 5178 5234 1870
Size: 1607 212 673 816 56 4545
Fragments arranged by size:
4545 1607 816 673 212 56
XmnI GAAnn'nnTTC
Cuts at: 3824 4304 6038 3824
Size: 480 1734 5695
Fragments arranged by size:
5695 1734 480
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3775 5717 276
Size: 53 83 186 936 2241 1942 2468
Fragments arranged by size:
2468 2241 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacII SapI SfiI
SrfI SwaI