Restriction map for
pFN21AA0377
(Circular) (Six-base) MAPSORT of: pFN21AA0377.seq Check: 921 from: 1 to: 8923
pFN21AA0377 8923 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6733 278
Size: 53 83 186 936 5197 2468
Fragments arranged by size:
5197 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 4101 6266 1937
Size: 849 1315 2165 4594
Fragments arranged by size:
4594 2165 1315 849
Acc65I G'GTAC_C
Cuts at: 6244 6244
Size: 8923
AclI AA'CG_TT
Cuts at: 1563 7050 7423 1563
Size: 5487 373 3063
Fragments arranged by size:
5487 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2835 3443 3587 4702 5488 5559 6060 6986
Size: 1203 608 144 1115 786 71 501 926
Cuts at: 6986 8370 8638 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1203 1115 926 786 608 501
268 144 71
AfeI AGC'GCT
Cuts at: 5282 5282
Size: 8923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8726
AflIII A'CryG_T
Cuts at: 1203 4496 5530 7822 8799 1203
Size: 3293 1034 2292 977 1327
Fragments arranged by size:
3293 2292 1327 1034 977
AgeI A'CCGG_T
Cuts at: 7754 7754
Size: 8923
AhdI GACnn_n'nnGTC
Cuts at: 4093 7652 4093
Size: 3559 5364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8923
AlwNI CAG_nnn'CTG
Cuts at: 6666 8238 6666
Size: 1572 7351
ApaI G_GGCC'C
Cuts at: 4904 4904
Size: 8923
ApaLI G'TGCA_C
Cuts at: 6980 8136 6980
Size: 1156 7767
ApoI r'AATT_y
Cuts at: 1493 5132 5524 6232 6458 6610 8719 1493
Size: 3639 392 708 226 152 2109 1697
Fragments arranged by size:
3639 2109 1697 708 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7477 160
Size: 872 6445 1606
Fragments arranged by size:
6445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8923
AvaI C'yCGr_G
Cuts at: 1870 1943 4461 4981 6248 1870
Size: 73 2518 520 1267 4545
Fragments arranged by size:
4545 2518 1267 520 73
AvrII C'CTAG_G
Cuts at: 5047 5047
Size: 8923
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3554 3587 3554
Size: 33 8890
BamHI G'GATC_C
Cuts at: 2454 4678 6253 6620 2454
Size: 2224 1575 367 4757
Fragments arranged by size:
4757 2224 1575 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 5071 5647 6244 7699
Size: 325 217 627 294 2990 576 597 1455
Cuts at: 7699 618
Size: 1842
Fragments arranged by size:
2990 1842 1455 627 597 576 325 294
217
BanII G_rGCy'C
Cuts at: 1391 2127 4904 5346 5598 6242 1391
Size: 736 2777 442 252 644 4072
Fragments arranged by size:
4072 2777 736 644 442 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 2619 3407 4370 4537 928
Size: 971 654 66 788 963 167 5314
Fragments arranged by size:
5314 971 963 788 654 167 66
BbvCI CC'TCA_GC
Cuts at: 3129 3609 3129
Size: 480 8443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3032 3066 7114 7148 1903
Size: 34 1095 34 4048 34 3678
Fragments arranged by size:
4048 3678 1095 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6815 8025 6815
Size: 1210 7713
BclI T'GATC_A
Cuts at: 1548 4030 5509 1548
Size: 2482 1479 4962
Fragments arranged by size:
4962 2482 1479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5325 7534 136
Size: 107 122 71 631 605 3653 2209 1525
Fragments arranged by size:
3653 2209 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3723 8918 3723
Size: 5195 3728
BlpI GC'TnA_GC
Cuts at: 2644 5747 6340 2644
Size: 3103 593 5227
Fragments arranged by size:
5227 3103 593
Bme1580I G_kGCm'C
Cuts at: 1790 2084 4759 4904 6984 8140 1790
Size: 294 2675 145 2080 1156 2573
Fragments arranged by size:
2675 2573 2080 1156 294 145
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6654 7612 442
Size: 416 53 83 399 24 5237 958 1753
Fragments arranged by size:
5237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3555 4487 3555
Size: 932 7991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2670 2814 3311 3389 3455 3666 4181
Size: 846 585 144 497 78 66 211 515
Cuts at: 4181 5258 5549 5930 7583 1239
Size: 1077 291 381 1653 2579
Fragments arranged by size:
2579 1653 1077 846 585 515 497 381
291 211 144 78 66
Bpu10I CC'TnA_GC
Cuts at: 3129 3318 3609 3129
Size: 189 291 8443
Fragments arranged by size:
8443 291 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 3293 3704 3895 5158 5338 7048 7913 8211
Size: 1143 411 191 1263 180 1710 865 298
Cuts at: 8211 2150
Size: 2862
Fragments arranged by size:
2862 1710 1263 1143 865 411 298 191
180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 5564 5953 7586 882
Size: 617 3066 999 389 1633 2219
Fragments arranged by size:
3066 2219 1633 999 617 389
BsaAI yAC'GTr
Cuts at: 493 1204 5246 5533 6714 493
Size: 711 4042 287 1181 2702
Fragments arranged by size:
4042 2702 1181 711 287
BsaBI GATnn'nnATC
Cuts at: 4649 8917 4649
Size: 4268 4655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6730 7112 7747 275
Size: 53 83 186 936 5197 382 635 1451
Fragments arranged by size:
5197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2451 3465 7356 7754 8028 8175 8586 2451
Size: 1014 3891 398 274 147 411 2788
Fragments arranged by size:
3891 2788 1014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2265 2295 4820 4850 1178
Size: 30 165 30 862 30 2525 30 5251
Fragments arranged by size:
5251 2525 862 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2667 2762 3410 3626 4361 4814 4930
Size: 967 514 95 648 216 735 453 116
Cuts at: 4930 5693 5936 6068 6194 6227 1186
Size: 763 243 132 126 33 3882
Fragments arranged by size:
3882 967 763 735 648 514 453 243
216 132 126 116 95 33
BseYI C'CCAG_C
Cuts at: 2077 2639 3616 4887 5387 5415 6034 8126 8622
Size: 562 977 1271 500 28 619 2092 496
Cuts at: 8622 2077
Size: 2378
Fragments arranged by size:
2378 2092 1271 977 619 562 500 496
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 3893 4262 1163
Size: 573 892 1265 369 5824
Fragments arranged by size:
5824 1265 892 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6373 7134 8162 1540
Size: 275 186 4372 761 1028 2301
Fragments arranged by size:
4372 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2499 4473 5346 5590 5598 6984 7069 8140
Size: 372 1974 873 244 8 1386 85 1071
Cuts at: 8140 2127
Size: 2910
Fragments arranged by size:
2910 1974 1386 1071 873 372 244 85
8
BsmI GAATG_Cn'
Cuts at: 2531 4086 6434 6527 2531
Size: 1555 2348 93 4927
Fragments arranged by size:
4927 2348 1555 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 3698 4295 4505 2672
Size: 1026 597 210 7090
Fragments arranged by size:
7090 1026 597 210
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2127 2499 4473 4759 4904 5346
Size: 399 294 43 372 1974 286 145 442
Cuts at: 5346 5590 5598 6242 6984 7069 8140 1391
Size: 244 8 644 742 85 1071 2174
Fragments arranged by size:
2174 1974 1071 742 644 442 399 372
294 286 244 145 85 43 8
BspEI T'CCGG_A
Cuts at: 8586 8586
Size: 8923
BspHI T'CATG_A
Cuts at: 6812 8542 6812
Size: 1730 7193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4113 4416 6278 844
Size: 3269 303 1862 3489
Fragments arranged by size:
3489 3269 1862 303
BsrBI CCG'CTC
Cuts at: 1394 1823 6810 1394
Size: 429 4987 3507
Fragments arranged by size:
4987 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3186 4871 5792 7418 7592 65
Size: 1183 1938 1685 921 1626 174 1396
Fragments arranged by size:
1938 1685 1626 1396 1183 921 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4998 7567 7754 1700
Size: 110 3188 2569 187 2869
Fragments arranged by size:
3188 2869 2569 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8923
BssSI C'ACGA_G
Cuts at: 4421 6983 7995 4421
Size: 2562 1012 5349
Fragments arranged by size:
5349 2562 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8923
BstBI TT'CG_AA
Cuts at: 2386 4492 7727 2386
Size: 2106 3235 3582
Fragments arranged by size:
3582 3235 2106
BstEII G'GTnAC_C
Cuts at: 1184 5810 1184
Size: 4626 4297
BstXI CCAn_nnnn'nTGG
Cuts at: 3061 5387 5807 3061
Size: 2326 420 6177
Fragments arranged by size:
6177 2326 420
BstYI r'GATC_y
Cuts at: 1972 2454 3523 3723 4068 4206 4678 6045 6253
Size: 482 1069 200 345 138 472 1367 208
Cuts at: 6253 6620 7005 7022 8474 8560 8572 8918 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1367 1069 482 472 385 367
346 345 208 200 138 86 17 12
BstZ17I GTA'TAC
Cuts at: 2787 2787
Size: 8923
Bsu36I CC'TnA_GG
Cuts at: 2520 3945 4896 6051 6177 6210 2520
Size: 1425 951 1155 126 33 5233
Fragments arranged by size:
5233 1425 1155 951 126 33
BtgI C'CryG_G
Cuts at: 513 1065 2040 2086 2901 3107 3120 4953 5067
Size: 552 975 46 815 206 13 1833 114
Cuts at: 5067 513
Size: 4369
Fragments arranged by size:
4369 1833 975 815 552 206 114 46
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3876 487
Size: 683 56 2650 5534
Fragments arranged by size:
5534 2650 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2701 4759 5096 5434 6439 7233 7253 2320
Size: 381 2058 337 338 1005 794 20 3990
Fragments arranged by size:
3990 2058 1005 794 381 338 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8888
DraI TTT'AAA
Cuts at: 6227 6227
Size: 8923
DraIII CAC_nnn'GTG
Cuts at: 3895 3895
Size: 8923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7930 809
Size: 507 6614 1802
Fragments arranged by size:
6614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2309 2412
Size: 54 1425 50 275 18 189 290 103
Cuts at: 2412 2916 3595 5018 5326 6370 7259 8888 8
Size: 504 679 1423 308 1044 889 1629 43
Fragments arranged by size:
1629 1425 1423 1044 889 679 504 308
290 275 189 103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6370 1537
Size: 275 4558 4090
Fragments arranged by size:
4558 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2460 3824 4175 4792 5256 6036 6853
Size: 750 357 1364 351 617 464 780 817
Cuts at: 6853 1353
Size: 3423
Fragments arranged by size:
3423 1364 817 780 750 617 464 357
351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3698 7510 7884 8030 387
Size: 1064 138 2109 3812 374 146 1280
Fragments arranged by size:
3812 2109 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 5344 5596 2125
Size: 3219 252 5452
Fragments arranged by size:
5452 3219 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2670 2814 2835 3311 3389 3443
Size: 393 453 585 144 21 476 78 54
Cuts at: 3443 3455 3587 3666 4181 4702 5258 5488 5549
Size: 12 132 79 515 521 556 230 61
Cuts at: 5549 5559 5930 6060 6986 7583 8370 8638 1239
Size: 10 371 130 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 597 585 556 521 515
476 453 393 371 268 230 144 132
130 79 78 61 54 21 12 10
EcoO109I rG'GnC_Cy
Cuts at: 3778 4087 4900 4901 5704 5767 5830 6205 3778
Size: 309 813 1 803 63 63 375 6496
Fragments arranged by size:
6496 813 803 375 309 63 63 1
EcoRI G'AATT_C
Cuts at: 5132 6232 6610 5132
Size: 1100 378 7445
Fragments arranged by size:
7445 1100 378
EcoRV GAT'ATC
Cuts at: 2882 3343 4198 2882
Size: 461 855 7607
Fragments arranged by size:
7607 855 461
FspI TGC'GCA
Cuts at: 7429 7429
Size: 8923
HaeII r_GCGC'y
Cuts at: 1678 4192 5158 5284 5747 8070 1678
Size: 2514 966 126 463 2323 2531
Fragments arranged by size:
2531 2514 2323 966 463 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2866 2871 2898 2903 3430
Size: 32 135 32 1294 5 27 5 527
Cuts at: 3430 3462 4079 4111 4415 4447 4670 4702 4911
Size: 32 617 32 304 32 223 32 209
Cuts at: 4911 4943 4961 4993 5474 5501 5506 5533 6146
Size: 32 18 32 481 27 5 27 613
Cuts at: 6146 6178 7564 7596 7638 7670 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 1294 617 613 527 481 304
223 209 135 42 32 32 32 32
32 32 32 32 32 32 32 27
27 27 18 5 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4438 6267 6513 669
Size: 988 281 2500 1829 246 3079
Fragments arranged by size:
3079 2500 1829 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2381 3504 748
Size: 1633 1123 6167
Fragments arranged by size:
6167 1633 1123
HpaI GTT'AAC
Cuts at: 6513 6513
Size: 8923
KpnI G_GTAC'C
Cuts at: 6248 6248
Size: 8923
MfeI C'AATT_G
Cuts at: 3476 6522 3476
Size: 3046 5877
MluI A'CGCG_T
Cuts at: 4496 8799 4496
Size: 4303 4620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4130 8037 8221 1305
Size: 2825 3907 184 2007
Fragments arranged by size:
3907 2825 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2311 2414 2918 5328 10
Size: 54 1425 343 479 103 504 2410 3605
Fragments arranged by size:
3605 2410 1425 504 479 343 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 3167 3815 4425 5030 5163 5805
Size: 648 1908 93 648 610 605 133 642
Cuts at: 5805 6881 7240 7399 518
Size: 1076 359 159 2042
Fragments arranged by size:
2042 1908 1076 648 648 642 610 605
359 159 133 93
MspA1I CmG'CkG
Cuts at: 1606 2648 3421 3882 3961 6038 6339 7016 8164
Size: 1042 773 461 79 2077 301 677 1148
Cuts at: 8164 8409 1606
Size: 245 2120
Fragments arranged by size:
2120 2077 1148 1042 773 677 461 301
245 79
NcoI C'CATG_G
Cuts at: 513 1065 2901 3107 4953 5067 513
Size: 552 1836 206 1846 114 4369
Fragments arranged by size:
4369 1846 1836 552 206 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8923
NotI GC'GGCC_GC
Cuts at: 6370 6370
Size: 8923
NspI r_CATG'y
Cuts at: 1132 1229 3640 5823 6281 7826 1132
Size: 97 2411 2183 458 1545 2229
Fragments arranged by size:
2411 2229 2183 1545 458 97
PasI CC'CwG_GG
Cuts at: 1348 5576 1348
Size: 4228 4695
PciI A'CATG_T
Cuts at: 7822 7822
Size: 8923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2765 1108
Size: 1657 7266
PfoI T'CCnGG_A
Cuts at: 3327 4071 4840 5565 6006 3327
Size: 744 769 725 441 6244
Fragments arranged by size:
6244 769 744 725 441
PmeI GTTT'AAAC
Cuts at: 6227 6227
Size: 8923
PmlI CAC'GTG
Cuts at: 5533 5533
Size: 8923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6992 7024 8505 8537 4682
Size: 32 2278 32 1481 32 5068
Fragments arranged by size:
5068 2278 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4087 5704 5767 5830 6205 4087
Size: 1617 63 63 375 6805
Fragments arranged by size:
6805 1617 375 63 63
PshAI GACnn'nnGTC
Cuts at: 1613 2261 1613
Size: 648 8275
PsiI TTA'TAA
Cuts at: 6493 6493
Size: 8923
PspOMI G'GGCC_C
Cuts at: 4900 4900
Size: 8923
PspXI vC'TCGA_Gb
Cuts at: 4981 4981
Size: 8923
PstI C_TGCA'G
Cuts at: 830 2243 2463 4110 4413 5188 6195 6275 830
Size: 1413 220 1647 303 775 1007 80 3478
Fragments arranged by size:
3478 1647 1413 1007 775 303 220 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8923
PvuII CAG'CTG
Cuts at: 2648 3421 3882 3961 6038 2648
Size: 773 461 79 2077 5533
Fragments arranged by size:
5533 2077 773 461 79
SacI G_AGCT'C
Cuts at: 2127 5346 5598 2127
Size: 3219 252 5452
Fragments arranged by size:
5452 3219 252
SalI G'TCGA_C
Cuts at: 1936 6265 1936
Size: 4329 4594
SbfI CC_TGCA'GG
Cuts at: 2463 6275 2463
Size: 3812 5111
ScaI AGT'ACT
Cuts at: 3979 7171 8883 3979
Size: 3192 1712 4019
Fragments arranged by size:
4019 3192 1712
SexAI A'CCwGG_T
Cuts at: 3379 3379
Size: 8923
SfcI C'TryA_G
Cuts at: 826 1045 2239 2459 3920 4098 4106 4142 4409
Size: 219 1194 220 1461 178 8 36 267
Cuts at: 4409 5184 5475 6191 6271 7406 8087 8278 826
Size: 775 291 716 80 1135 681 191 1471
Fragments arranged by size:
1471 1461 1194 1135 775 716 681 291
267 220 219 191 178 80 36 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8923
SmaI CCC'GGG
Cuts at: 1872 6250 1872
Size: 4378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3308 3719 3874 4981 5137
Size: 197 926 186 1179 411 155 1107 156
Cuts at: 5137 5353 7027 7928 8190 820
Size: 216 1674 901 262 1553
Fragments arranged by size:
1674 1553 1179 1107 926 901 411 262
216 197 186 156 155
SnaBI TAC'GTA
Cuts at: 493 6714 493
Size: 6221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8923
SphI G_CATG'C
Cuts at: 1132 6281 1132
Size: 5149 3774
SspI AAT'ATT
Cuts at: 5 52 6847 5
Size: 47 6795 2081
Fragments arranged by size:
6795 2081 47
StuI AGG'CCT
Cuts at: 1517 4987 5219 5553 1517
Size: 3470 232 334 4887
Fragments arranged by size:
4887 3470 334 232
StyI C'CwwG_G
Cuts at: 513 1065 2056 2901 3107 3251 3774 4816 4953
Size: 552 991 845 206 144 523 1042 137
Cuts at: 4953 5047 5067 5297 5554 6362 513
Size: 94 20 230 257 808 3074
Fragments arranged by size:
3074 1042 991 845 808 552 523 257
230 206 144 137 94 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3264 3823 4076 5038 5429 6950 6967
Size: 1255 1684 559 253 962 391 1521 17
Cuts at: 6967 7120 7305 7750 325
Size: 153 185 445 1498
Fragments arranged by size:
1684 1521 1498 1255 962 559 445 391
253 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3430 3977 4455
Size: 274 80 33 51 1443 1453 547 478
Cuts at: 4455 7169 8881 96
Size: 2714 1712 138
Fragments arranged by size:
2714 1712 1453 1443 547 478 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4663 4695 4820 4852 8883 8915 4663
Size: 32 125 32 4031 32 4671
Fragments arranged by size:
4671 4031 125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2731 4226 1316
Size: 1415 1495 6013
Fragments arranged by size:
6013 1495 1415
XbaI T'CTAG_A
Cuts at: 3790 6259 3790
Size: 2469 6454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2587 2908 3781 5086 5573 5990 2054
Size: 533 321 873 1305 487 417 4987
Fragments arranged by size:
4987 1305 873 533 487 417 321
XhoI C'TCGA_G
Cuts at: 1943 4981 1943
Size: 3038 5885
XmaI C'CCGG_G
Cuts at: 1870 6248 1870
Size: 4378 4545
XmnI GAAnn'nnTTC
Cuts at: 3979 7052 3979
Size: 3073 5850
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6731 276
Size: 53 83 186 936 5197 2468
Fragments arranged by size:
5197 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BbeI BfrBI BsiWI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI