Restriction map for
pFN21AA0371
(Circular) (Six-base) MAPSORT of: pFN21AA0371.seq Check: 1137 from: 1 to: 8299
pFN21AA0371 8299 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2374 5308 2374
Size: 2934 5365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6109 278
Size: 53 83 186 936 4573 2468
Fragments arranged by size:
4573 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5642 1937
Size: 3705 4594
Acc65I G'GTAC_C
Cuts at: 4466 5620 4466
Size: 1154 7145
AclI AA'CG_TT
Cuts at: 1563 3418 5598 6426 6799 1563
Size: 1855 2180 828 373 3063
Fragments arranged by size:
3063 2180 1855 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2357 2886 3446 4641 5180 5322 6362
Size: 446 279 529 560 1195 539 142 1040
Cuts at: 6362 7746 8014 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1195 1040 560 539 529 446
279 268 142
AfeI AGC'GCT
Cuts at: 3189 3189
Size: 8299
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8102
AflIII A'CryG_T
Cuts at: 1203 3602 4545 4736 7198 8175 1203
Size: 2399 943 191 2462 977 1327
Fragments arranged by size:
2462 2399 1327 977 943 191
AgeI A'CCGG_T
Cuts at: 3378 7130 3378
Size: 3752 4547
AhdI GACnn_n'nnGTC
Cuts at: 7028 7028
Size: 8299
AleI CACnn'nnGTG
Cuts at: 1166 3758 4016 1166
Size: 2592 258 5449
Fragments arranged by size:
5449 2592 258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3125 1184
Size: 1941 6358
AlwNI CAG_nnn'CTG
Cuts at: 2646 2972 3088 4805 5095 5158 5185 6042 7614
Size: 326 116 1717 290 63 27 857 1572
Cuts at: 7614 2646
Size: 3331
Fragments arranged by size:
3331 1717 1572 857 326 290 116 63
27
ApaI G_GGCC'C
Cuts at: 4584 4584
Size: 8299
ApaLI G'TGCA_C
Cuts at: 3100 3774 6356 7512 3100
Size: 674 2582 1156 3887
Fragments arranged by size:
3887 2582 1156 674
ApoI r'AATT_y
Cuts at: 1493 3368 5608 5834 5986 8095 1493
Size: 1875 2240 226 152 2109 1697
Fragments arranged by size:
2240 2109 1875 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6853 160
Size: 872 5821 1606
Fragments arranged by size:
5821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
AvaI C'yCGr_G
Cuts at: 1870 1943 2838 5624 1870
Size: 73 895 2786 4545
Fragments arranged by size:
4545 2786 895 73
AvrII C'CTAG_G
Cuts at: 4000 4000
Size: 8299
BamHI G'GATC_C
Cuts at: 3979 5629 5996 3979
Size: 1650 367 6282
Fragments arranged by size:
6282 1650 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2676 3794 4028 4466 4793
Size: 325 217 627 889 1118 234 438 327
Cuts at: 4793 5620 7075 618
Size: 827 1455 1842
Fragments arranged by size:
1842 1455 1118 889 827 627 438 327
325 234 217
BanII G_rGCy'C
Cuts at: 1391 2589 4320 4584 5618 1391
Size: 1198 1731 264 1034 4072
Fragments arranged by size:
4072 1731 1198 1034 264
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4199 928
Size: 971 2300 5028
Fragments arranged by size:
5028 2300 971
BbvCI CC'TCA_GC
Cuts at: 4059 4207 4059
Size: 148 8151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3755 3789 6490 6524 1903
Size: 34 1818 34 2701 34 3678
Fragments arranged by size:
3678 2701 1818 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6191 7401 6191
Size: 1210 7089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3966 4130 6910
Size: 107 122 71 631 605 2294 164 2780
Cuts at: 6910 136
Size: 1525
Fragments arranged by size:
2780 2294 1525 631 605 164 122 107
71
BglII A'GATC_T
Cuts at: 2052 2380 4598 8294 2052
Size: 328 2218 3696 2057
Fragments arranged by size:
3696 2218 2057 328
BlpI GC'TnA_GC
Cuts at: 3285 3953 5716 3285
Size: 668 1763 5868
Fragments arranged by size:
5868 1763 668
Bme1580I G_kGCm'C
Cuts at: 1790 3104 3778 4584 4798 6360 7516 1790
Size: 1314 674 806 214 1562 1156 2573
Fragments arranged by size:
2573 1562 1314 1156 806 674 214
BmgBI CAC'GTC
Cuts at: 1318 2872 1318
Size: 1554 6745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3419 4247 4436
Size: 416 53 83 399 24 2002 828 189
Cuts at: 4436 5499 6030 6988 442
Size: 1063 531 958 1753
Fragments arranged by size:
2002 1753 1063 958 828 531 416 399
189 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4296 5314 4296
Size: 1018 7281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2551 3993 4325 4391 4832 5192 5437 5536
Size: 1312 1442 332 66 441 360 245 99
Cuts at: 5536 6959 1239
Size: 1423 2579
Fragments arranged by size:
2579 1442 1423 1312 441 360 332 245
99 66
Bpu10I CC'TnA_GC
Cuts at: 3818 4059 4207 3818
Size: 241 148 7910
Fragments arranged by size:
7910 241 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2565 4945 6424 7289 7587 2051
Size: 514 2380 1479 865 298 2763
Fragments arranged by size:
2763 2380 1479 865 514 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2442 2853 4505 6962 882
Size: 617 943 411 1652 2457 2219
Fragments arranged by size:
2457 2219 1652 943 617 411
BsaAI yAC'GTr
Cuts at: 493 1204 6090 493
Size: 711 4886 2702
Fragments arranged by size:
4886 2702 711
BsaBI GATnn'nnATC
Cuts at: 2088 2757 3216 8293 2088
Size: 669 459 5077 2094
Fragments arranged by size:
5077 2094 669 459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6106 6488 7123 275
Size: 53 83 186 936 4573 382 635 1451
Fragments arranged by size:
4573 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3378 6732 7130 7404 7551 7962 3378
Size: 3354 398 274 147 411 3715
Fragments arranged by size:
3715 3354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5165 5195 1178
Size: 30 165 30 3762 30 4282
Fragments arranged by size:
4282 3762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3009 3088 4115 4270 4319 1186
Size: 1823 79 1027 155 49 5166
Fragments arranged by size:
5166 1823 1027 155 79 49
BseYI C'CCAG_C
Cuts at: 3252 3784 3908 4649 5310 5483 7502 7998 3252
Size: 532 124 741 661 173 2019 496 3553
Fragments arranged by size:
3553 2019 741 661 532 496 173 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2476 3539 3744 1163
Size: 573 740 1063 205 5718
Fragments arranged by size:
5718 1063 740 573 205
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2804 3227 5749 6510 7538 1540
Size: 275 186 803 423 2522 761 1028 2301
Fragments arranged by size:
2522 2301 1028 803 761 423 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2589 3104 3778 3936 6360 6445 7516 2129
Size: 460 515 674 158 2424 85 1071 2912
Fragments arranged by size:
2912 2424 1071 674 515 460 158 85
BsmI GAATG_Cn'
Cuts at: 2249 3011 3500 5810 5903 2249
Size: 762 489 2310 93 4645
Fragments arranged by size:
4645 2310 762 489 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2589 3104 3778 3936 4320 4584
Size: 399 339 460 515 674 158 384 264
Cuts at: 4584 4798 5618 6360 6445 7516 1391
Size: 214 820 742 85 1071 2174
Fragments arranged by size:
2174 1071 820 742 674 515 460 399
384 339 264 214 158 85
BspEI T'CCGG_A
Cuts at: 7962 7962
Size: 8299
BspHI T'CATG_A
Cuts at: 6188 7918 6188
Size: 1730 6569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2374 3741 4129 5308 5654 844
Size: 1530 1367 388 1179 346 3489
Fragments arranged by size:
3489 1530 1367 1179 388 346
BsrBI CCG'CTC
Cuts at: 1394 1823 6186 1394
Size: 429 4363 3507
Fragments arranged by size:
4363 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2160 2692 2697 4795 4873 5580 6794
Size: 1183 912 532 5 2098 78 707 1214
Cuts at: 6794 6968 65
Size: 174 1396
Fragments arranged by size:
2098 1396 1214 1183 912 707 532 174
78 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 3226 3378 3822 3891 3936 6943 7130
Size: 110 1416 152 444 69 45 3007 187
Cuts at: 7130 1700
Size: 2869
Fragments arranged by size:
3007 2869 1416 444 187 152 110 69
45
BsrGI T'GTAC_A
Cuts at: 96 5070 96
Size: 4974 3325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8299
BssSI C'ACGA_G
Cuts at: 3335 6359 7371 3335
Size: 3024 1012 4263
Fragments arranged by size:
4263 3024 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2283 2402 5523 794
Size: 1489 119 3121 3570
Fragments arranged by size:
3570 3121 1489 119
BstBI TT'CG_AA
Cuts at: 4393 7103 4393
Size: 2710 5589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8299
BstXI CCAn_nnnn'nTGG
Cuts at: 2687 3760 2687
Size: 1073 7226
BstYI r'GATC_y
Cuts at: 1972 2052 2380 3115 3979 4598 5629 5996 6381
Size: 80 328 735 864 619 1031 367 385
Cuts at: 6381 6398 7850 7936 7948 8294 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1031 864 735 619 385 367
346 328 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 4200 4200
Size: 8299
BtgI C'CryG_G
Cuts at: 513 1065 2004 4641 513
Size: 552 939 2637 4171
Fragments arranged by size:
4171 2637 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7560
Fragments arranged by size:
7560 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 3238 3443 3732 5374 5422 5815 6609 6629
Size: 911 205 289 1642 48 393 794 20
Cuts at: 6629 2327
Size: 3997
Fragments arranged by size:
3997 1642 911 794 393 289 205 48
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2548 2583 529
Size: 35 1984 35 6245
Fragments arranged by size:
6245 1984 35 35
DraI TTT'AAA
Cuts at: 2488 5603 2488
Size: 3115 5184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7306 809
Size: 507 5990 1802
Fragments arranged by size:
5990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3224 3361 3885
Size: 54 1425 50 275 18 1394 137 524
Cuts at: 3885 5367 5746 6635 8264 8
Size: 1482 379 889 1629 43
Fragments arranged by size:
1629 1482 1425 1394 889 524 379 275
137 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3224 5746 1537
Size: 275 1412 2522 4090
Fragments arranged by size:
4090 2522 1412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2333 2390 4395 5156 6229 1353
Size: 654 326 57 2005 761 1073 3423
Fragments arranged by size:
3423 2005 1073 761 654 326 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6886 7260 7406 387
Size: 1064 138 5297 374 146 1280
Fragments arranged by size:
5297 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2587 2587
Size: 8299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2357 2551 2886 3446 3993 4325
Size: 393 446 279 194 335 560 547 332
Cuts at: 4325 4391 4641 4832 5180 5192 5322 5437 5536
Size: 66 250 191 348 12 130 115 99
Cuts at: 5536 6362 6959 7746 8014 1239
Size: 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 560 547 446 393
348 335 332 279 268 250 194 191
130 115 99 66 12
EcoO109I rG'GnC_Cy
Cuts at: 2643 3331 4162 4345 4375 4581 4680 4815 2643
Size: 688 831 183 30 206 99 135 6127
Fragments arranged by size:
6127 831 688 206 183 135 99 30
EcoRI G'AATT_C
Cuts at: 5608 5986 5608
Size: 378 7921
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3521 4448 4796 3521
Size: 927 348 7024
Fragments arranged by size:
7024 927 348
FspI TGC'GCA
Cuts at: 6805 6805
Size: 8299
HaeII r_GCGC'y
Cuts at: 1678 3191 3898 3943 5095 7446 1678
Size: 1513 707 45 1152 2351 2531
Fragments arranged by size:
2531 2351 1513 1152 707 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2172 2204 2372 2404 2786
Size: 32 135 32 600 32 168 32 382
Cuts at: 2786 2818 2942 2974 3100 3132 4246 4278 5048
Size: 32 124 32 126 32 1114 32 770
Cuts at: 5048 5080 6940 6972 7014 7046 1373
Size: 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1860 1114 770 600 382 168 135
126 124 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 5643 5889 669
Size: 988 281 885 2820 246 3079
Fragments arranged by size:
3079 2820 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 2781 2936 748
Size: 2033 155 6111
Fragments arranged by size:
6111 2033 155
HpaI GTT'AAC
Cuts at: 5889 5889
Size: 8299
KpnI G_GTAC'C
Cuts at: 4470 5624 4470
Size: 1154 7145
MfeI C'AATT_G
Cuts at: 5898 5898
Size: 8299
MluI A'CGCG_T
Cuts at: 8175 8175
Size: 8299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2383 2565 2950 5110 5619 7413 7597 1305
Size: 1078 182 385 2160 509 1794 184 2007
Fragments arranged by size:
2160 2007 1794 1078 509 385 184 182
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3363 3887 10
Size: 54 1425 343 1531 524 4422
Fragments arranged by size:
4422 1531 1425 524 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3107 3553 3758 4016 4477 4550 5066
Size: 648 1941 446 205 258 461 73 516
Cuts at: 5066 5205 5339 5379 6257 6616 6775 518
Size: 139 134 40 878 359 159 2042
Fragments arranged by size:
2042 1941 878 648 516 461 446 359
258 205 159 139 134 73 40
MspA1I CmG'CkG
Cuts at: 1606 2071 2706 2969 3223 3771 4924 5310 5715
Size: 465 635 263 254 548 1153 386 405
Cuts at: 5715 6392 7540 7785 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1153 1148 677 635 548 465 405
386 263 254 245
NaeI GCC'GGC
Cuts at: 3824 3893 3824
Size: 69 8230
NcoI C'CATG_G
Cuts at: 513 1065 2004 4641 513
Size: 552 939 2637 4171
Fragments arranged by size:
4171 2637 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8299
NgoMIV G'CCGG_C
Cuts at: 3822 3891 3822
Size: 69 8230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8299
NotI GC'GGCC_GC
Cuts at: 5746 5746
Size: 8299
NspI r_CATG'y
Cuts at: 1132 1229 2474 3206 3606 4549 5065 5657 7202
Size: 97 1245 732 400 943 516 592 1545
Cuts at: 7202 1132
Size: 2229
Fragments arranged by size:
2229 1545 1245 943 732 592 516 400
97
PasI CC'CwG_GG
Cuts at: 1348 4353 1348
Size: 3005 5294
PciI A'CATG_T
Cuts at: 3602 4545 7198 3602
Size: 943 2653 4703
Fragments arranged by size:
4703 2653 943
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3274 1108
Size: 2166 6133
PfoI T'CCnGG_A
Cuts at: 2076 2645 3118 5151 2076
Size: 569 473 2033 5224
Fragments arranged by size:
5224 2033 569 473
PmeI GTTT'AAAC
Cuts at: 5603 5603
Size: 8299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6368 6400 7881 7913 6368
Size: 32 1481 32 6754
Fragments arranged by size:
6754 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 3331 4162 4345 4375 2643
Size: 688 831 183 30 6567
Fragments arranged by size:
6567 831 688 183 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8299
PsiI TTA'TAA
Cuts at: 5869 5869
Size: 8299
PspOMI G'GGCC_C
Cuts at: 4580 4580
Size: 8299
PstI C_TGCA'G
Cuts at: 830 3438 3902 4943 5285 5429 5651 830
Size: 2608 464 1041 342 144 222 3478
Fragments arranged by size:
3478 2608 1041 464 342 222 144
PvuI CG_AT'CG
Cuts at: 2001 2804 2001
Size: 803 7496
PvuII CAG'CTG
Cuts at: 2071 2969 3771 4924 5310 2071
Size: 898 802 1153 386 5060
Fragments arranged by size:
5060 1153 898 802 386
SacI G_AGCT'C
Cuts at: 2589 2589
Size: 8299
SalI G'TCGA_C
Cuts at: 1936 5641 1936
Size: 3705 4594
SbfI CC_TGCA'GG
Cuts at: 5651 5651
Size: 8299
ScaI AGT'ACT
Cuts at: 6547 8259 6547
Size: 1712 6587
SfcI C'TryA_G
Cuts at: 826 1045 2671 3434 3898 4539 4939 5281 5425
Size: 219 1626 763 464 641 400 342 144
Cuts at: 5425 5527 5647 6782 7463 7654 826
Size: 102 120 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 763 681 641 464 400
342 219 191 144 120 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
SgrAI Cr'CCGG_yG
Cuts at: 1700 3936 1700
Size: 2236 6063
SmaI CCC'GGG
Cuts at: 1872 5626 1872
Size: 3754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2580 2838 4960 6403 7304
Size: 197 926 87 550 258 2122 1443 901
Cuts at: 7304 7566 820
Size: 262 1553
Fragments arranged by size:
2122 1553 1443 926 901 550 262 258
197 87
SnaBI TAC'GTA
Cuts at: 493 6090 493
Size: 5597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8299
SphI G_CATG'C
Cuts at: 1132 3206 5657 1132
Size: 2074 2451 3774
Fragments arranged by size:
3774 2451 2074
SspI AAT'ATT
Cuts at: 5 52 6223 5
Size: 47 6171 2081
Fragments arranged by size:
6171 2081 47
StuI AGG'CCT
Cuts at: 1517 4205 1517
Size: 2688 5611
StyI C'CwwG_G
Cuts at: 513 1065 2004 3567 4000 4585 4641 4714 5738
Size: 552 939 1563 433 585 56 73 1024
Cuts at: 5738 513
Size: 3074
Fragments arranged by size:
3074 1563 1024 939 585 552 433 73
56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4229 4879 6326 6343 6496 6681 7126
Size: 1255 2649 650 1447 17 153 185 445
Cuts at: 7126 325
Size: 1498
Fragments arranged by size:
2649 1498 1447 1255 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 3235 5070
Size: 274 80 33 51 1443 579 679 1835
Cuts at: 5070 6545 8257 96
Size: 1475 1712 138
Fragments arranged by size:
1835 1712 1475 1443 679 579 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 4512 4544 8259 8291 3332
Size: 32 1148 32 3715 32 3340
Fragments arranged by size:
3715 3340 1148 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8299
XbaI T'CTAG_A
Cuts at: 5635 5635
Size: 8299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2724 2724
Size: 8299
XhoI C'TCGA_G
Cuts at: 1943 2838 1943
Size: 895 7404
XmaI C'CCGG_G
Cuts at: 1870 5624 1870
Size: 3754 4545
XmnI GAAnn'nnTTC
Cuts at: 4428 5444 6428 4428
Size: 1016 984 6299
Fragments arranged by size:
6299 1016 984
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6107 276
Size: 53 83 186 936 4573 2468
Fragments arranged by size:
4573 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI